KR101719261B1 - Sulfur-cabbage - Google Patents

Abstract

The present invention relates to a manufacturing method of a soil conditioner manufactured with sulfur to cultivate sulfur-cabbage, a cultivation method of sulfur-cabbage using the same, and the sulfur-cabbage, wherein the method manufactures a soil conditioner by mixing and fermenting expellant sulfur powder, manufactured by fermenting and decomposing toxic sulfur by adding an effective microorganism culture medium, rice bran, phyllite, and bentonite, implants a cabbage seeding in which a cabbage seed sown in a seed raising box is raised in a field for the manufactured soil conditioner to be foliage treated, and irrigation nutrient solution treating a liquefied treating agent in which the effective microorganism culture medium is diluted to the cabbage implanted in the field.

Description

{SULFUR-CABBAGE} A method for producing a soil conditioner using sulfur, a method for cultivating sulfur cabbage using a soil conditioner and soil conditioner prepared by the method,

The present invention relates to a method for producing a soil amendment agent produced using sulfur, a method for cultivating sulfuric Chinese cabbage using the soil amendment agent and soil amendment agent produced by the method, and a sulfur cabbage thereof, and more particularly, Produced from 1983 in Japan EM culture institute) was added to the fermentation broth to produce a soil remediation agent prepared by mixing fermented broth, fermented broth, rice bran, bentonite, fermented broth and sowing box The cabbage seedlings seeded with Chinese cabbage seeds were transplanted into the field, treated with the soil remediation agent prepared using the sulfur produced above, and treated with a liquid treating agent diluted with Effective Microorganisms A method for producing a soil amendment agent produced by using sulfur treated to grow sulfuric cabbage and a soil prepared by the method How to grow Chinese cabbage sulfur with amendments and soil conditioner and to its Cabbage sulfur.

Sulfur is a substance which is high in heat and toxic in Donguibogam, but it is a substance that releases the cold air in the body to help the lack of Yang, and regulates the accumulation and fraud of the heart and releases the erysipelas. In addition, according to Chinese classical literature, sulfur is the main ingredient of gold dust, which is known as the golden salt of the world, which defeats all kinds of illnesses, "It is known that the evaluation of herbal medicine is great, as it is delivered as a potion of rejuvenation. In addition to sulfur, it is not only a beneficial agent but also a heavy metal, pesticide and other chemicals which are becoming a cause of cancer and various incurable diseases. It is known as an excellent detoxifying substance.

Sulfur having the above effect is highly toxic to heat and can not be used as a medicine without legal process (legal process). In order to remove the toxicity of sulfur, the sulfur collected from nature is put into the poison container, Sulfur was used to reduce the toxicity by pulverizing it by heating. However, the sulfur produced by the method of removing the toxicity of the above sulfur is very small in the efficiency of reducing the toxicity, There was no.

Chinese cabbage is one of the two major vegetables in Korea. It is the main raw material of kimchi. It is very high in moisture content, rich in fiber, rich in nutrients such as calcium and vitamin C, and vitamins in cabbage are boiled or fried When compared to other vegetables, the loss is small, it plays a big role in nutrient supply.

Chinese cabbage is a cold-tolerant crop. The optimum temperature for growth is 15 ~ 20 ℃. The cultivation of Chinese cabbage seeds are seeded on seedlings, and they are planted on the mainland for about 20 to 30 days, and harvested about 60 to 70 days after planting. The date of sowing and harvesting depends on the breed, but it takes about 60 to 100 days.

Korean Patent No. 1276904 discloses a cultivation method of high-calcium Chinese cabbage, and Korean Patent Laid-Open Publication No. 2014-0065560 discloses a cultivation method of Chinese cabbage wherein a microbial compost using a mushroom waste medium is cultivated in a Chinese cabbage. However, there is no technique for cultivating Chinese cabbage using the virgin sulfur as in the present invention.

In order to solve the above-mentioned problems, the present invention relates to a method for preparing a fermentation broth by mixing fermentation broth prepared by adding fermentation broth with a useful microorganism (produced from EM Japan 1983) to toxic sulfur, And a method for making a soil improving agent produced by fermentation and using sulfur.

Another object of the present invention is to provide a method of cultivating sulfuric cabbage having excellent growth effect and enhanced mineral content such as calcium, magnesium and sulfur by using the soil amendment agent prepared by the above method, and to provide the sulfuric cabbage.

In order to achieve the above object, the present invention provides a method for cultivating sulfuric cabbage using a soil conditioner produced using sulfur according to the present invention, comprising fermenting a mixed medium comprising green tea leaves and cinnamon leaves and cultivating 15 kinds of useful microorganisms Lactobacillus parafarrhinis , Lactobacillus paracasei , Lactobacillus tolerans , Lactobacillus buchneri , Lactobacillus harbinensis , Lactobacillus spp ., Lactobacillus spp ., Lactobacillus spp . Lactobacillus perolens , Lactobacillus rhamnosus , Lactobacillus vaccinostercus , Acetobacter lovaniensis , and Acetobacter peroxydans have been found to be 2.2 x 10 < RTI ID = 0.0 > ⁸ cfu / ml, yeast Pichia pertansa fermentans), Candida ethanol Rica (Candida ethanolica) and Saccharomyces Rommie Cobb cis Scoring Enigma (Saccharomycopsis schoenii) is 2.4 × 10 ⁴ cfu / ㎖, bacteria, thio Bacillus thio oxy residence (Thiobacillus thiooxidans) and Bacillus Turing Gen System (Bacillus thuringiensis) Were each inoculated with 2.4 × 10 ⁴ cfu / ml) and incubated at 25 ° C. for 92-140 hours. Toxic sulfur powder was added to the resulting microorganism solution for primary fermentation, and then the fermentation lysate was dissolved in a wet colloid mill After crushing, the crushed primary fermentation product is subjected to solid-liquid separation, the fermentation broth of the useful microorganism is added to the separated precipitate, and the fermented product is subjected to second fermentation and decomposition. Then, the secondary fermented product is subjected to solid- (S200) preparing a soil conditioner prepared by using sulfur produced by mixing and fermenting the prepared virgin gasoline powder with rice bran, phyllite, and bentonite; A step (S210) of implanting a Chinese cabbage seedlings planted with seeds of Chinese cabbage seeds in a seedling box into a field; (S220) adding minerals to the step of transplanting the cabbage seedlings into a field; A step S230 of subjecting the soil remediation agent prepared using sulfur to a foliar treatment and adding a liquid treating agent diluted with the Lactobacillus paraparangiosis culture liquid to a Chinese cabbage grafted in a field, .

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In addition, after 25 to 35 days after implantation of the Chinese cabbage seedlings seeded with the Chinese cabbage seeds for 20 to 30 days in the seedling box, 0.05 to 0.15 kg of the soil amendment agent prepared using sulfur was sprayed at intervals of 10 to 15 days , And subjecting the liquid treating agent prepared by diluting the Lactobacillus paraparangiuric culture liquid to a Chinese cabbage grafted in a field at an interval of 3 to 5 days.

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Further, the minerals are characterized by being at least one or more minerals selected from the group consisting of calcium, magnesium and sulfur.

The sulfuric cabbage cultivated with the soil improver prepared by using the sulfur according to the present invention was prepared by fermenting a mixed medium consisting of green tea leaves and cinnamon leaves and adding 15 kinds of useful microorganisms such as Lactobacillus parafarraginis , , Lactobacillus paracasei , Lactobacillus tolerans , Lactobacillus buchneri , Lactobacillus harbinensis , Lactobacillus perolens , Lactobacillus spp ., Lactobacillus spp ., Lactobacillus spp . Lactobacillus rhamnosus , Lactobacillus vaccinostercus , Acetobacter lovaniensis and Acetobacter peroxydans contained 2.2 x 10 < ⁸ > cfu / ml, peomen Tansu (Pichia fermentans), Candida ethanol Rica (Candida ethanolica ) And Saccharomycopsis schoenii (2.4 × 10 ⁴ cfu / ml), and the bacterium Thiobacillus thiooxidans and Bacillus thuringiensis were 2.4 × 10 ⁴ cfu / ml And incubated at 25 DEG C for 92 to 140 hours. Toxic sulfur powder was added to the useful microorganism solution to perform primary fermentation, and then the fermentation broth was broken with a wet colloid mill. The pulverized primary fermentation product , And then the fermentation broth of the second fermentation product was subjected to solid-liquid separation of the second fermented sulfuric acid fermentation broth, and then the separated crude liquor was separated into dry granulated sulfur powder, , A plant improvement agent prepared by using sulfur produced by mixed fermentation of bentonite, a seedling of cabbage was transplanted into a field to add minerals, The soil conditioner is subjected to foliar treatment and the liquid treatment agent diluted with the Lactobacillus paraparangiosis culture liquid is cultivated by treating the cabbage transplanted in the field with a nutrient solution.

The present invention relates to a soil remediation agent prepared by mixing sulfuric acid powder prepared by fermentation with a useful microbial culture for toxic sulfur, rice bran, fennel and bentonite, fermented by using sulfur, Can be used to effectively remove toxic substances of sulfur by fermentation and decomposition by adding a microbial culture to a toxic sulfur, and using the soil amendment agent prepared by using the virgin sulfur powder and a useful microorganism (produced from EM Japan 1983) The treated liquid treatment agent is treated in the cultivation of Chinese cabbage to grow the Chinese cabbage, thereby increasing the growth of the Chinese cabbage and increasing the mineral content in the cabbage, thereby producing high quality Chinese cabbage.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a schematic view showing a method for producing a soil conditioner according to the present invention. FIG.
FIG. 2 is a schematic view showing a method of cultivating a sulfuric Chinese cabbage cultivated with a soil remediation agent produced using sulfur according to the present invention. FIG.
Fig. 3 is a photograph showing a planting of sulfur cabbage. Fig.
4 is a photograph of a sulfuric cabbage grown by the method of the present invention.
Figs. 5 and 6 are tables of mineral constituents of sulfur cabbages grown by the method of the present invention.

For a better understanding of the present invention, a preferred embodiment of the present invention will be described with reference to the accompanying drawings.

The embodiments of the present invention may be modified in various forms, and the scope of the present invention should not be construed as being limited to the embodiments described in detail below. The present embodiments are provided to explain the present invention more safely to those having ordinary skill in the art.

Therefore, the shapes and the like of the elements in the drawings can be exaggeratedly expressed to emphasize a clearer description. It should be noted that in the drawings, the same members are denoted by the same reference numerals.

FIG. 1 is a schematic view showing a method for producing a soil amendment agent according to the present invention, FIG. 2 is a schematic view showing a method of cultivating a sulfuric Chinese cabbage cultivated with a soil improving agent using sulfur according to the present invention, FIG. FIG. 4 is a photograph of a sulfuric cabbage cultivated by the method of the present invention. FIG.

The method for producing a soil conditioner using sulfur according to the present invention, the cultivation method of the sulfuric Chinese cabbage using the same, and the sulfuric Chinese cabbage will be described in detail with reference to examples shown in FIG. 1 to FIG.

The soil improving agent prepared by using the sulfur according to the present invention will now be described with reference to FIG.

[Example 1]

[Step S100 of fermenting the mixed medium]

Mixed media containing green tea leaves and camphor leaves as a carbon source is fermented.

The mixed medium containing green tea leaves and camphor leaves as a carbon source at a weight ratio of 6.5 to 7.5: 2.5 to 3.5 is fermented at 23 to 35 ° C for 43 to 48 days.

The mixed medium may be prepared by mixing 1.5 to 2.5 parts by weight of ionized mineral based on 100 parts by weight of a mineral mixture, 8 to 12 parts by weight of natural fruit juice, , 2.7 to 3.3 parts by weight of natural vinegar, 4.5 to 5.5 parts by weight of salt, 2.7 to 3.3 parts by weight of sweetening components and 74 to 84 parts by weight of molasses. But is not limited thereto.

[Step S110 of cultivating a useful microorganism by inoculation]

In the step of fermenting the mixed medium, a molasses dilution solution sterilized at high temperature is added to a mixed medium fermented, and the useful microorganisms are inoculated and cultured.

A 2 to 3% (w / v) molasses dilution is used for the molasses dilution.

As described above, the fermented mixed medium may be prepared by adding a molasses dilution solution sterilized at high temperature and then inoculating the useful microorganism and culturing the mixture at 23 to 35 ° C for 92 to 140 hours, but the present invention is not limited thereto.

The useful microorganism may preferably be a mixed microorganism consisting of lactic acid bacteria, yeast and bacteria, more preferably Lactobacillus parafarrhinis , Lactobacillus paracasei , Lactobacillus tolerans ), Lactobacillus buchneri , Lactobacillus harbinensis , Lactobacillus perolens , Lactobacillus rhamnosus , Lactobacillus vaccinostercus , Lactobacillus spp . Acetobacter lovaniens s), Acetobacter peroxydans , Pichia fermentans), Candida ethanol Rica (Candida ethanolica), Saccharomyces Rommie Cobb Scoring System Enigma (Saccharomycopsis schoenii ), thiobacillus thiooxidans , and Bacillus thuringiensis , but are not limited thereto. The preparation of the virgin sulfur powder using the above strains could be made into the most appropriate adduct sulfur powder for cabbage growth.

[First fermentation decomposition step (S120)]

It is preferable that the toxic sulfur powder is added to the useful microorganism culture solution in which the beneficial microorganism is inoculated and cultured and then primary fermentation is performed at 25 to 35 ° C for 22 to 26 hours.

To 1000 kg of the above-mentioned useful microorganism culture solution, 230-270 kg of toxic sulfur powder is added.

[Step S130 of crushing the primary fermentation broth with a wet colloid mill]

In the primary fermentation decomposition step, the primary fermentation broth is subjected to solid-liquid separation, and the separated precipitate is pulverized with a wet colloid mill.

The crushing of the primary fermentation broth makes the primary fermentation broth finer so as to increase the contact area between the sulfur powder and the useful microorganisms, thereby more effectively eliminating the toxicity of sulfur.

[Second fermentation decomposition step (S140)]

The step of crushing the primary fermentation broth with a wet colloid mill is carried out by solid-liquid separation of the pulverized primary fermentation broth, adding the useful microorganism culture liquid obtained by inoculating and culturing the useful microorganism into the separated precipitate, I will.

[Step S150 of fermenting the mixture by mixing the mixture with the virgin sulfur powder]

In the second fermentation and decomposition step, the second order hydrolyzed sulfuric acid hydrolyzate is subjected to solid-liquid separation, and the separated solid is dried to obtain a mixture of the virgin sulfur powder, the rice bran, the phyllite and the bentonite, Ferment.

The mixture weight of the virgin sulfur powder and the virgin sulfur powder as described above is preferably composed of 230 to 270 kg of virgin sulfur powder, 230 to 270 kg of crude steel, 230 to 270 kg of phyllite, and 230 to 270 kg of bentonite The mixture can be fermented at 22 to 28 ° C for 66 to 78 hours. More preferably, 250 kg of virgin sulfur powder, 250 kg of rice bran, 250 kg of phyllotamine and 250 kg of bentonite are mixed and then fermented at 25 ° C. for 72 hours Can be manufactured.

The cultivation of Chinese cabbage using the soil conditioner prepared under the above manufacturing conditions promoted the growth of Chinese cabbage and cultivated as a Chinese cabbage containing a large amount of functional ingredients.

[Example 2]

Manufacture of Admiral Sulfur Powder

1. Preparation of useful microorganism culture liquid

6 liters of a carbon source mixture containing green tea leaves and camphor leaves at a weight ratio of 7: 3 was added with 2% of ionized minerals, 10% of natural juice, 3% of natural vinegar, 5% of salt, 3% of sweet potatoes and 77% Min and a pH of 4.8-5 was prepared and fermented at 25 DEG C for 45 days.

The fermented mixed medium was added with a 2% to 3% (w / v) molasses dilution which had been sterilized at high temperature so as to have a ratio of 0.6% (v / v) and titrated to a pH of 3.2 to 3.7.

Then beneficial microorganisms 15 kinds of lactic acid bacteria is Lactobacillus parapa than Nice (Lactobacillus parafarraginis), Lactobacillus para casei (Lactobacillus paracasei), Lactobacillus tolreo Lance (Lactobacillus tolerans), Lactobacillus boots Tenerife (Lactobacillus buchneri), Lactobacillus Har binen Lactobacillus < / RTI > harbinensis , Lactobacillus perolens , Lactobacillus rhamnosus , Lactobacillus vaccinostercus , Acetobacter lovaniensis and Acetobacterperoxidans (Acetobacter peroxydans) is 2.2 × 10 ⁸ cfu / ㎖, yeast Pichia peomen Tansu (Pichia fermentans), Candida ethanol Rica (Candida ethanolica) and Saccharomyces Rommie Cobb cis Scoring Enigma (Saccharomycopsis schoenii) is 2.4 × 10 ⁴ cfu / ㎖ , bacteria of the Bacillus thio thio oxy residence (Thiobacillus thiooxidans) and Bacillus Harlingen sheath (Bacillus thuringiensis) is incubated for each 2.4 × 10 ⁴ cfu / ㎖ included in) the inoculated In 25 ℃ 92 ~ 140 hours.

2. Addition of Toxic Sulfur Powder, First and Second Admiral Fermentation with Useful Microorganism Culture Solution

After adding 300 kg of toxic sulfur powder to 1 ton of the useful microorganism culture, primary fermentation decomposition was carried out at 25 to 35 ° C for 24 hours.

The primary fermentation broth was disrupted with a wet colloid mill for 48 hours to produce 1000 mesh particles.

The crushed primary fermentation broth was subjected to solid-liquid separation, and 1 kg of a useful microorganism culture was added to 300 kg of the separated precipitate, followed by secondary fermentation decomposition at 25 to 35 ° C for 24 hours.

The second degummed sulfur fermentation broth was subjected to solid-liquid separation, and the separated solid was dried at 80 ° C for 10 hours to prepare an adducted sulfur powder.

The cultivation method of the sulfuric Chinese cabbage using the soil remediation agent prepared using the sulfur according to the present invention will now be described with reference to FIG. 2 through FIG. 4. FIG.

[Example 1]

[Step S200 of preparing a soil conditioner prepared using sulfur]

The mixed medium containing green tea leaves and camphor leaves is fermented with a carbon source.

The mixed medium containing green tea leaves and camphor leaves as a carbon source at a weight ratio of 6.5 to 7.5: 2.5 to 3.5 is fermented at 23 to 35 ° C for 43 to 48 days.

The mixed medium may be prepared by mixing 1.5 to 2.5 parts by weight of ionized mineral based on 100 parts by weight of a mineral mixture, 8 to 12 parts by weight of natural fruit juice, , 2.7 to 3.3 parts by weight of natural vinegar, 4.5 to 5.5 parts by weight of salt, 2.7 to 3.3 parts by weight of a sweetener component and 74 to 84 parts by weight of molasses was added to prepare a mixed medium of pH 4.8 to 5.2 prepared by adding a mineral mixture .

In the step of fermenting the mixed medium, a molasses dilution solution sterilized at high temperature is added to a mixed medium fermented, and the useful microorganisms are inoculated and cultured.

As described above, the fermented mixed medium is prepared by adding a molasses dilution solution sterilized at high temperature, inoculating the useful microorganisms, and culturing at 23 to 35 ° C for 92 to 140 hours.

The useful microorganism may preferably be a mixed microorganism consisting of lactic acid bacteria, yeast and bacteria, more preferably Lactobacillus paraparangiosis (Lactobacillus parafarraginis), Lactobacillus paracasei (Lactobacillus paracasei), Lactobacillus tollarans (Lactobacillus tolerance), Lactobacillus bootseri (Lactobacillus buchneri), Lactobacillus hirvinensis (Lactobacillus harbinensis), Lactobacillus ferulensis (Lactobacillus perolens), Lactobacillus lambatus (Lactobacillus rhamnosus), Lactobacillus buccinostercus (Lactobacillus vaccinostercus), Acetobacter lavanniensisAcetobacter lovaniensis), acetobarperoxidans (Acetobacter peroxydans), Pichia permentans (Pichia fermentans), Candida ethanolica (Candida ethanolica), Saccharomyces cisisconi (Saccharomycopsis schoenii), Thiobacillus thiooxidans (Thiobacillus thiooxidans) And Bacillus turgensis (Bacillus thuringiensis), But is not limited thereto. The preparation of the virgin sulfur powder using the above strains could be made into the most appropriate adduct sulfur powder for cabbage growth.

A step S120 of adding a toxic sulfur powder to the useful microorganism culture solution and then performing primary fermentation and decomposition in the step of inoculating and culturing the useful microorganism;

In addition, the toxic sulfur powder is added to the useful microorganism culture solution in which the useful microorganisms are inoculated and cultured, followed by primary fermentation decomposition for 22 to 26 hours at 25 to 35 ° C.

In the primary fermentation decomposition step, the primary fermentation lysate is disrupted with a wet colloid mill.

The step of crushing the primary fermentation broth with a wet colloid mill is carried out by solid-liquid separation of the pulverized primary fermentation broth, adding the useful microorganism culture liquid obtained by inoculating and culturing the useful microorganism into the separated precipitate, do.

In the second fermentation and decomposition step, the second order hydrolyzed sulfuric acid hydrolyzate is subjected to solid-liquid separation, and the separated solid is dried to obtain a mixture of the virgin sulfur powder, the rice bran, the phyllite and the bentonite, Fermented.

The mixture weight of the virgin sulfur powder and the virgin sulfur powder as described above is preferably composed of 230 to 270 kg of virgin sulfur powder, 230 to 270 kg of crude steel, 230 to 270 kg of phyllite, and 230 to 270 kg of bentonite The mixture can be fermented at 22 to 28 ° C for 66 to 78 hours. More preferably, 250 kg of virgin sulfur powder, 250 kg of rice bran, 250 kg of phyllotamine and 250 kg of bentonite are mixed and then fermented at 25 ° C. for 72 hours Can be manufactured. The cultivation of Chinese cabbage using the soil conditioner prepared under the above manufacturing conditions promoted the growth of Chinese cabbage and cultivated as a Chinese cabbage containing a large amount of functional ingredients.

[Transplanting the cabbage seedlings into the field (S210)]

I plant the cabbage seedlings that planted the seeds of cabbage seeds in the seedling box.

[Step S220 of adding minerals]

Minerals are added to the step of transplanting the cabbage seedlings into the field.

In addition, the minerals are composed of at least one mineral selected from the group consisting of calcium, magnesium and sulfur.

[Step S230 of processing the liquid nutrient solution to the Chinese cabbage transplanted in the field with the liquid processing agent diluted with the culture liquid]

In the step of adding the minerals, a soil conditioner prepared using sulfur is subjected to foliar treatment and a liquid treating agent diluted with a Lactobacillus paraparangiosis culture liquid is subjected to cross-contamination treatment with Chinese cabbage grafted in a field.

More preferably, the Chinese cabbage seeds sown in the seedling boxes are cultivated for 20 to 30 days, and after 25 to 35 days after the transplanting of the cabbage seedlings into the fields, the soil conditioner is applied at a rate of 0.05 to 0.15 kg per 3 to 4 times And the liquid treatment agent diluted with Lactobacillus paraparangiosis culture liquid can be treated with the liquid culture of the cabbage at intervals of 3 to 5 days, more preferably, the cabbage seeds sown in the cabbage box for 25 days After 30 days after transplanted Chinese cabbage seedlings into the field, 0.1 kg of soil amendment was treated with foliar treatment three to four times at intervals of 10 to 15 days, and the liquid treatment agent diluted with Lactobacillus paraparauginus culture was transferred into a field To be treated at the interval of 3 ~ 5 days. Cultivation of the Chinese cabbage under the above conditions promoted the growth of the Chinese cabbage, increased the mineral content in the cabbage, and cultivated the cabbage as an excellent Chinese cabbage.

More specifically, the method for cultivating the sulfuric cabbage comprises fermenting a mixed medium containing green tea leaves and camphor leaves at a weight ratio of 7: 3 as a carbon source at 25 ° C for 45 days, adding a molasses dilution solution which has been sterilized at high temperature to a fermented mixed medium Then, a useful microorganism is inoculated and cultured at 25 DEG C for 92 to 140 hours. Toxic sulfur powder is added to the useful microorganism culture solution, followed by primary fermentation decomposition for 24 hours at 25 to 35 DEG C, The fermentation broth was crushed by a colloid mill. The crushed primary fermentation broth was subjected to solid-liquid separation, and a useful microorganism culture solution was added to the separated precipitate, followed by secondary fermentation decomposition for 24 hours at 25 to 35 ° C, 250 kg of virgin sulfur powder prepared by drying the separated solid at 80 ° C, 250 kg of rice bran, 250 kg of phylloxerum and 250 kg of bentonite were mixed and fermented at 25 ° C for 72 hours, ; 30 days after transplanting the Chinese cabbage seedlings planted in the seedling box for 25 days, the soil amendment agent prepared by using sulfur was treated with 0.1 kg per hectare for 3-4 times at intervals of 10 ~ 15 days, And a step of subjecting the liquid treating agent prepared by diluting the Lactobacillus paraparangiaceous culture liquid to the Chinese cabbage grafted in the field at intervals of 3 to 5 days.

In the cultivation method of sulfuric Chinese cabbage according to the present invention, the virgin sulfur powder produced under the above-mentioned conditions can be easily and easily prepared with a relatively low cost by using the useful microorganism culture liquid, It is water-soluble and has a high water absorption rate, so that the effect is maximized even with a small amount of addition.

The sulfuric cabbage cultivated with the soil improver produced by using the sulfur according to the present invention is prepared by fermenting a mixed medium consisting of green tea leaves and camphor leaves and cultivating a useful microorganism in the mixed medium to produce a useful microorganism solution, , The fermentation lysate is disrupted with a wet colloid mill, the lysate of the primary fermentation product is subjected to solid-liquid separation, and the fermentation lysate is subjected to a secondary fermentation by adding a useful microorganism culture solution to the separated precipitate, And a soil remediation agent prepared by using sulfur produced by fermentation of mixed liquor, which is prepared by mixing solid crude liquor and crude liquor, and a mixture of rice bran, fennel, and bentonite.

Using the soil remediation agent described above, the seedling of the cabbage seedlings was transplanted into a field to add minerals, the soil remediation agent prepared using sulfur was subjected to foliar treatment, and the liquid treating agent diluted with the Lactobacillus paraparagneus culture was transplanted into the field A Chinese cabbage is cultivated by treating a Chinese cabbage with a nutrient solution.

Hereinafter, embodiments of the present invention will be described in detail. However, the following examples are for illustrative purposes only and are not intended to limit the scope of the present invention.

[Example 2]

Conditions for cultivation of sulfur cabbage using soil remediation agent using sulfur material Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4

soil
Improving agent Admiral sulfur powder 250 kg 1000 kg 250 kg 350 kg 150 kg Rice bran 250 kg - 480 kg 150 kg 350 kg Phyllite 250 kg - - 350 kg 150 kg Bentonite 250 kg - - 150 kg 350 kg Humic acid - - 20 kg - - Oyster shell - - 250 kg - - Liquid phase
Treatment agent Lactobacillus paraparaginis culture medium 810 L 810 L 810 L 810 L 810 L Admiral sulfur powder - - 250 kg - - Caustic soda - - 200 kg - - Abalone shell - - 10 kg - - Cortical shell - - 5 kg - - rock salt - - 15 kg - - Sodium silicate - - 20 L - -

Experimental Example 1: Comparison of mineral content according to cultivation conditions of sulfur cabbage using a soil amendment agent manufactured with sulfur

Table 1 shows the mineral contents of Chinese cabbage according to the cultivation conditions of sulfur cabbage as shown in Table 2 below. Comparative Example 5 means a cabbage cultivated by the method of Example 2, but cultivated without treating the soil conditioner and the liquid treating agent. As a result, iron (Fe) and zinc (Zn) did not show a large difference in all treatments, and sodium content was low in Comparative Example 1 and Comparative Example 5, 4 showed high contents. It was also confirmed that the content of calcium (Ca), magnesium (Mg) and sulfur (S) was the highest in the cabbage cultivated by the method of Example 2.

Example 2: Cultivation of sulfur Chinese cabbage

250 kg of the virgin sulfur powder prepared in Example 1, 250 kg of rice bran, 250 kg of fenugreek and 250 kg of bentonite were mixed and fermented at 25 ° C for 72 hours to prepare a soil conditioner. 30 days after transplanting the cabbage seedlings seeded with the Chinese cabbage seeds in the seedling box for 25 days, the prepared soil conditioner was subjected to foliar treatment at a rate of 0.1 to 4 kg per 10-15 days at intervals of 3 to 4 times, Water was added to the Lactobacillus parafarrhinis culture (2.2 × 10 ⁸ cfu / ml) and diluted to 1/500 (v / v) was added to the transplanted cabbage at about 4.05 L per hectare for 3-5 days The sulphurous cabbage was cultivated by treatment with the nutrient solution at intervals.

Comparison of mineral content according to growing conditions of sulfur cabbage (㎎ / 100 g) Types of minerals Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Ca 484.7 282.6 332.4 412.5 425.6 312.3 K 812.6 754.6 802.5 810.2 803.4 818.8 Fe 0.9 0.7 0.6 1.0 1.1 2.8 Mg 53.8 27.4 24.1 42.3 41.7 35.0 Na 26.7 6.8 24.6 25.7 26.8 5.8 Zn 1.1 0.9 0.3 1.0 1.1 1.1 S 191.7 161.8 154.6 146.5 142.3 71.3

Experimental Example 2: Growth characteristics of Chinese cabbage according to the cultivation conditions of sulfur cabbage

The growth of Chinese cabbage according to the cultivation conditions of sulfur cabbage was examined and the results are shown in Table 3 below. The harvested Chinese cabbage was cultivated for 60 days after harvesting, and the cabbage cultivated in accordance with the method of Example 2 was observed on weekdays, And the growth of leaf width was enhanced.

Studies on the Growth of Chinese Cabbage According to the Conditions of Sulfur Chinese Cabbage Cultivation division Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Weekday (g) 3,902 3,651 3,621 3,700 3,695 3,578 (G) 2,642 2,512 2,433 2,568 2,541 2,498 Leaf length (cm) 42.6 41.8 41.6 41.3 41.2 41.0 Leaf width (cm) 31.2 29.6 28.9 30.2 30.0 28.4 Number of leaves () 79.1 78.2 79.0 79.6 79.4 78.6

It will be apparent to those skilled in the art that various modifications and equivalent arrangements may be made therein without departing from the scope of the present invention. . It is therefore to be understood that the invention is not limited to the form set forth in the detailed description.

Accordingly, the true scope of the present invention should be determined by the technical idea of the appended claims. It is also to be understood that the invention includes all modifications, equivalents, and alternatives falling within the spirit and scope of the invention as defined by the appended claims.

S100: Step of fermenting the mixed medium
S110: step of inoculating and cultivating a useful microorganism
S120: step of primary fermentation decomposition
S130: a step of disrupting the primary fermentation broth with a wet colloid mill
S140: step of secondary fermentation decomposition
S150: Step of fermenting the mixture by mixing the mixture with the virgin sulfur powder
S200: Preparation of a soil conditioner prepared using sulfur
S210: Transplanting the cabbage seedlings into the field
S220: Step of adding minerals
S230: a step of cultivating the liquid processing agent diluted with the culture liquid to the Chinese cabbage implanted in the field,

Claims (10)

delete delete delete delete delete delete Green tea leaves and cinnamon leaves were fermented, and 15 kinds of useful microorganisms Lactobacillus parafarraginis , Lactobacillus paracasei , Lactobacillus tolerans ( Lactobacillus paracasei , ), Lactobacillus buchneri , Lactobacillus harbinensis , Lactobacillus perolens , Lactobacillus rhamnosus , Lactobacillus vaccinostercus , Lactobacillus spp . acetonitrile bakteo Rover it N-Sys (Acetobacter lovaniensis) and acetonitrile bakteo peroxy thiooxidans (Acetobacter peroxydans) is 2.2 × 10 ⁸ cfu / ㎖, yeast Pichia peomen Tansu (Pichia fermentans), Candida ethanol Rica (Candida ethanolica) and Saccharomyces Rommie Cobb Scoring system Enigma (Saccharomycopsis schoenii) is 2.4 × 10 ⁴ cfu / ㎖, three The thio Bacillus thio oxy residence (Thiobacillus thiooxidans) and Bacillus Turing Gen System (Bacillus thuringiensis) is a useful microorganism solution of each 2.4 × 10 ⁴ included in cfu / ㎖) inoculation to produce cultured at 25 ℃ for 92-140 hours The fermentation lysate was disrupted with a wet colloid mill, and the pulverized primary fermentation product was subjected to solid-liquid separation. Secondary fermentation was carried out by adding a useful microorganism culture solution to the separated precipitate, followed by 2 Preparing a soil remediation agent prepared by using sulfur produced by fermenting mixed liquor with dry liquor prepared with the separated liquor separated from the secondary fermented digestive sulfuric acid hydrolyzate by fermentation with a mixture of rice bran, phyllite and bentonite; (S200);
A step (S210) of implanting a Chinese cabbage seedlings planted with seeds of Chinese cabbage seeds in a seedling box into a field;
(S220) adding minerals to the step of transplanting the cabbage seedlings into a field;
A step S230 of subjecting the soil remediation agent prepared using sulfur to a foliar treatment and adding a liquid treating agent diluted with the Lactobacillus paraparangiosis culture liquid to a Chinese cabbage grafted in a field, A method of cultivating sulfur Chinese cabbage using soil improving agent using sulfur. 8. The method of claim 7,
After 25 to 35 days of transplanting the Chinese cabbage seedlings seeded with the cabbage seeds seeded in the seedling box for 20 ~ 30 days, 0.05 ~ 0.15 kg of the soil amendment agent prepared by using sulfur was added at intervals of 10 ~ 15 days And a step of culturing the Chinese cabbage transplanted in the field with a liquid treating agent prepared by diluting the Lactobacillus paraparangiuric acid culture liquid at a time interval of 3 to 5 days by using a soil remediation agent using sulfur How to grow sulfur cabbage. 8. The method of claim 7,
Wherein the mineral is one or more minerals selected from the group consisting of calcium, magnesium and sulfur. Green tea leaves and cinnamon leaves were fermented, and 15 kinds of useful microorganisms Lactobacillus parafarraginis , Lactobacillus paracasei , Lactobacillus tolerans ( Lactobacillus paracasei , ), Lactobacillus buchneri , Lactobacillus harbinensis , Lactobacillus perolens , Lactobacillus rhamnosus , Lactobacillus vaccinostercus , Lactobacillus spp . acetonitrile bakteo Rover it N-Sys (Acetobacter lovaniensis) and acetonitrile bakteo peroxy thiooxidans (Acetobacter peroxydans) is 2.2 × 10 ⁸ cfu / ㎖, yeast Pichia peomen Tansu (Pichia fermentans), Candida ethanol Rica (Candida ethanolica) and Saccharomyces Rommie Cobb Scoring system Enigma (Saccharomycopsis schoenii) is 2.4 × 10 ⁴ cfu / ㎖, three The thio Bacillus thio oxy residence (Thiobacillus thiooxidans) and Bacillus Turing Gen System (Bacillus thuringiensis) is a useful microorganism solution of each 2.4 × 10 ⁴ included in cfu / ㎖) inoculation to produce cultured at 25 ℃ for 92-140 hours The fermentation lysate was disrupted with a wet colloid mill, and the pulverized primary fermentation product was subjected to solid-liquid separation. Secondary fermentation was carried out by adding a useful microorganism culture solution to the separated precipitate, followed by 2 Secondary sediment sulfuric acid fermentation broth was separated from the second fermentation broth by fermentation, and the soil was treated with sulfuric acid, Cabbage seedlings were transplanted into a field in the box to add minerals, foliar treatment of the soil amendment agent prepared using sulfur, and cultivation of Lactobacillus paraparauginis culture Wherein the diluted liquid treating agent is cultivated by treating the cabbage transplanted in the field with a nutrient solution.

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