KR100699333B1 - Antimicrobial composition containing the root extract of R. coreanus MIQ or R. crataegifolius - Google Patents

Antimicrobial composition containing the root extract of R. coreanus MIQ or R. crataegifolius Download PDF

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KR100699333B1
KR100699333B1 KR1020050005059A KR20050005059A KR100699333B1 KR 100699333 B1 KR100699333 B1 KR 100699333B1 KR 1020050005059 A KR1020050005059 A KR 1020050005059A KR 20050005059 A KR20050005059 A KR 20050005059A KR 100699333 B1 KR100699333 B1 KR 100699333B1
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genus
composition
bacteria
rubus
root extract
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KR20060084263A (en
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김원용
김재섭
김현아
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김원용
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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61FFILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F9/00Methods or devices for treatment of the eyes; Devices for putting-in contact lenses; Devices to correct squinting; Apparatus to guide the blind; Protective devices for the eyes, carried on the body or in the hand
    • A61F9/08Devices or methods enabling eye-patients to replace direct visual perception by another kind of perception
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61HPHYSICAL THERAPY APPARATUS, e.g. DEVICES FOR LOCATING OR STIMULATING REFLEX POINTS IN THE BODY; ARTIFICIAL RESPIRATION; MASSAGE; BATHING DEVICES FOR SPECIAL THERAPEUTIC OR HYGIENIC PURPOSES OR SPECIFIC PARTS OF THE BODY
    • A61H3/00Appliances for aiding patients or disabled persons to walk about
    • A61H3/06Walking aids for blind persons
    • A61H3/061Walking aids for blind persons with electronic detecting or guiding means
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61HPHYSICAL THERAPY APPARATUS, e.g. DEVICES FOR LOCATING OR STIMULATING REFLEX POINTS IN THE BODY; ARTIFICIAL RESPIRATION; MASSAGE; BATHING DEVICES FOR SPECIAL THERAPEUTIC OR HYGIENIC PURPOSES OR SPECIFIC PARTS OF THE BODY
    • A61H3/00Appliances for aiding patients or disabled persons to walk about
    • A61H3/06Walking aids for blind persons
    • A61H3/066Installations on the floor, e.g. special surfaces, to guide blind persons
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61HPHYSICAL THERAPY APPARATUS, e.g. DEVICES FOR LOCATING OR STIMULATING REFLEX POINTS IN THE BODY; ARTIFICIAL RESPIRATION; MASSAGE; BATHING DEVICES FOR SPECIAL THERAPEUTIC OR HYGIENIC PURPOSES OR SPECIFIC PARTS OF THE BODY
    • A61H3/00Appliances for aiding patients or disabled persons to walk about
    • A61H3/06Walking aids for blind persons
    • A61H3/068Sticks for blind persons
    • EFIXED CONSTRUCTIONS
    • E01CONSTRUCTION OF ROADS, RAILWAYS, OR BRIDGES
    • E01CCONSTRUCTION OF, OR SURFACES FOR, ROADS, SPORTS GROUNDS, OR THE LIKE; MACHINES OR AUXILIARY TOOLS FOR CONSTRUCTION OR REPAIR
    • E01C23/00Auxiliary devices or arrangements for constructing, repairing, reconditioning, or taking-up road or like surfaces
    • E01C23/16Devices for marking-out, applying, or forming traffic or like markings on finished paving; Protecting fresh markings
    • E01C23/20Devices for marking-out, applying, or forming traffic or like markings on finished paving; Protecting fresh markings for forming markings in situ
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61HPHYSICAL THERAPY APPARATUS, e.g. DEVICES FOR LOCATING OR STIMULATING REFLEX POINTS IN THE BODY; ARTIFICIAL RESPIRATION; MASSAGE; BATHING DEVICES FOR SPECIAL THERAPEUTIC OR HYGIENIC PURPOSES OR SPECIFIC PARTS OF THE BODY
    • A61H3/00Appliances for aiding patients or disabled persons to walk about
    • A61H3/06Walking aids for blind persons
    • A61H3/061Walking aids for blind persons with electronic detecting or guiding means
    • A61H2003/063Walking aids for blind persons with electronic detecting or guiding means with tactile perception
    • A61H2003/065Walking aids for blind persons with electronic detecting or guiding means with tactile perception in the form of braille
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection
    • Y02A50/30Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
    • Y02A50/46Medical treatment of waterborne diseases characterized by the agent
    • Y02A50/468The waterborne disease being caused by a bacteria
    • Y02A50/475The waterborne disease being caused by a bacteria of the genus Shigella, i.e. Dysentery

Abstract

The present invention relates to a root extract of Rubus sp. Plants having an antimicrobial activity and a composition comprising the same. More specifically, the present invention relates to a root extract of a plant of the genus Rubus having antibacterial activity and an antimicrobial composition comprising the extract.
Root extract of the plant of the genus Lubus according to the present invention is antibacterial against skin diseases, purulent diseases, surgical wound infections, urinary tract-inducing bacteria and food poisoning bacteria, anthrax, pests, caries, acne, methicillin-resistant staphylococci, dysentery and athlete's foot Because of its activity, purulent diseases such as skin infections, purulent, otitis media, cystitis, peritonitis, urinary tract infections, food poisoning, anthrax, plague, caries, acne, methicillin-resistant staphylococcal infections, bacterial dysentery and pathogenic fungi It can be very useful for the prevention or treatment of athlete's foot. In addition, the root extract of the plant of the genus Rubus of the present invention can be prepared in the form of cosmetics, disinfectants, sterile detergents and food preservatives.
Rubus genus, roots, antimicrobial activity

Description

Antimicrobial composition containing the root extract of bokbunja or raspberry {Antimicrobial composition containing the root extract of R. coreanus MIQ or R. crataegifolius}

1a to 1c is a photograph showing the antimicrobial activity of the ethanol extract of the root of bokbunja strawberry ( Rubus coreanus MIQ) according to the present invention against pathogenic bacteria.

Figure 2a to 2c is a photograph showing the antimicrobial activity against pathogenic bacteria and fungi of the ethanol extract of raspberry ( Rubus crataegifolius ) root according to the present invention.

The present invention relates to a root extract of Rubus sp. Plants having antimicrobial activity and a composition comprising the same. More specifically, the present invention relates to an antimicrobial composition comprising the extract and an extract of the plant root of the genus Rubus antibacterial activity.

Rubus sp. Is a deciduous tree belonging to the Rosaceae family and is classified into 200 to 300 species depending on scholars due to its diverse differentiation. Among them, medicinal examples include bokbunja strawberry ( R. coreanus MIQ), raspberry ( R. crataegifolius ) and locust strawberry ( R. parvifolius Linne var. Tiphyllus ). Fruits of the genus Rubus have been used for medicinal fruits, roots and leaves, in particular, Rubi fructus , a fruit of the genus Rubus, has been used in the folk medicine for cooling, cooling, jigging, tonic and diabetes. In addition, the leaves are effective for asthma and its roots are known to be effective for hemostasis, hematopoiesis, infertility, menstrual irregularities. Therefore, since the plant of the genus Rubus has a different pharmacological action depending on the site, it is predicted that there will be different components for each site. On the other hand, in relation to the pharmacological action of the roots of the plant of the genus Lubus , it has been reported in the literature that the alcohol extract of the roots of R. crataegifolius has an anti-inflammatory effect (Cao Y. et al., Zhongguo Zhong Yao Za Zhi , 21). (11): 687-8, 1996), crude extracts of raspberry roots have been reported to have anti-cancer effects (Lee JH et al., Arch Pharm Res ., 23 (4): 338-43 , 2000). However, the antimicrobial effect of plant roots of the genus Rubus has not been reported so far.

Most antimicrobial agents currently used are manufactured through chemical synthesis, which is expensive and has many limitations such as causing side effects. Therefore, in recent years, research has been actively conducted to separate new antimicrobial substances from natural products. The separation of new antimicrobial substances from natural products should take into account that the antimicrobial spectrum is broad and safe for long term administration without side effects.

Therefore, the present inventors completed the present invention by confirming that the root extract of the plant of Rubus genus has antibacterial activity against various pathogenic bacteria and fungi while researching for many years to separate new antimicrobial substances from natural products.

Accordingly, it is an object of the present invention to provide a root extract of a plant of the genus Rubus having antibacterial activity.

In addition, another object of the present invention to provide an antimicrobial composition comprising the extract.

In addition, another object of the present invention to provide an antimicrobial formulation comprising the extract.

In order to achieve the above object, the present invention provides a root extract of the plant of the genus Rubus antibacterial activity.

The present invention also provides an antimicrobial composition comprising the extract.

The present invention also provides an antimicrobial preparation comprising the extract.

Hereinafter, the present invention will be described in detail.

The root extract of Rubus sp. Plants according to the present invention is characterized by having antimicrobial activity against various pathogenic bacteria and fungi.

Preferably, the root extract of the plant of the genus Rubus of the present invention is Staphylococcus sp., Streptococcus sp., Bacillus sp., Yersinia sp. ), Propionibacterium sp., Shigella sp. Bacteria and Trichophyton sp. More preferably, the extract of the present invention is Staphylococcus aureus ( S. aureus ), including Methicillin Resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis , Staphylococcus bacteria such as S. intermedius and S. saprophyticus , Streptococcus bacteria such as Streptococcus mutans , Bacillus anthracis Bacillus bacteria such as B. anthracis , Yersian bacteria such as Y. pestis , Propionibacterium acnes and Propionibacterium escidopropionis (P. acidipropionici) and propionyl sludge tumefaciens bacteria belonging to the genus, sH Gela Prex Neri (Shigella flexneri) and Shh Gela in bacteria, such as tricot python men Tag It has a tricot python antimicrobial activity against fungi such as pies in test (Trichophyton mentagrophytes).

The Staphylococcus aureus is one of the bacteria that is widely distributed in the natural world, invading the skin, respiratory organs and genitourinary system, and is one of the representative causes of purulent diseases such as purulent, otitis media and cystitis as well as food poisoning. In particular, the bacteria are often infected with people with weak immunity, such as surgical patients, chronic bronchitis, cold patients. In particular, methicillin resistant Staphylococcus aureus is a strain that has lost sensitivity to methicillin and has a high level of resistance or resistance. In addition to the methicillin, the strain is widely used in various types of antibiotics such as penicillin antibiotics such as oxacillin, β-lactam antibiotics, aminoglycosides and macrolides. It is known to be resistant and is the leading cause of infection in the hospital.

The Staphylococcus epidermidis is a non-pathogenic bacterium that is commonly present in the skin or the nose. However, when the fluid is supplied through the vein, blood is collected, parasites in the catheter used in the surgical patient cause infection of the wound, skin tissue infection and peritonitis.

The Staphylococcus intermedius infects the skin or urinary tract of animals, especially dogs, causing cystitis or purulent dermatitis.

The Staphylococcus saprophyteus is known as a main cause of urinary tract infection in normal bacteria present in the human body or in women.

The Streptococcus mutans is a major causative agent of tooth decay, while in the oral cavity, it breaks down sugar and converts it into lactic acid, causing erosion of the tooth, which is the main component of lime.

Bacillus anthracis is a causative agent of anthrax, a common infectious disease. The anthrax is infected with most vertebrates and is known to have a high incidence of cattle or goats. In humans, most are infected when exposed to infected animals or their by-products. Infection with anthrax causes more than 95% of deaths if antibiotics are not given within the initial 24-48 hours after infection.

The Yersinian pestis is a bacterium that causes a plague that is an acute recessive epidemic as a bacillus or an interval bacterium. Plasma mortality is very high and humans are commonly known to be infected by contact with rat fleas, infected wildlife and plague patients. When the bacteria are infected, the lymph nodes spread to the lymph nodes, and the lymph nodes become very swelled, and the infection of the lungs causes fatal pulmonary edema.

The propionibacterium acnes and propionibacterium esidopropionis are anaerobic bacteria that reside in sebaceous glands and pores, causing sebum to break down into free fatty acids, causing acne.

The Shigella Prexeri is a small Gram-negative bacillus with no motility and is highly infectious, causing bacterial dysentery, which is mainly caused by high fever, nausea, convulsive abdominal pain, and discomfort after defecation in poor countries.

The tricophyton mentagrophytes is known as a causative bacterium that causes skin fungal infections such as athlete's foot as skin filamentous fungi.

In the present invention, as the root of the plant of the genus Rubus as medicinal and edible, for example, bokbunja strawberry ( R. coreanus MIQ), raspberry ( R. crataegifolius ) and locust ( R. parvifolius Linne var. Tiphyllus ) Strawberry ( R. coreanus for. Concolor T. Lee ), Gooseberry ( R. phoenicolasius Maximowicz ), Strawberries ( R. hirsutus Thunberg ), Surrey berries ( R. corchorifoius L Fil ), Strawberries ( R. ribesioideus Maisumira ), Winter Roots of strawberries ( R. buergeri Miquel ) and raspberries ( R. oldhamil Maximowicz ) are included. In an embodiment of the present invention, bokbunja strawberry ( R. coreanus MIQ) and raspberry ( R. crataegifolius ) roots are illustrated.

Roots of the plant in the rubus can be obtained from nature or commercially available in the herbal medicine. Root of the plant of the genus Rubus used in the preparation of the extract of the present invention is preferably to use the dry, it can be used to crushed with a grinder to increase the extraction efficiency. As a method of drying the roots of the plant in Lubus, both dry, shade, hot air drying, freeze drying and natural drying methods can be used. Preferably hot air drying and lyophilization methods can be used.

In addition, the root extract of the plant of the genus Rubus of the present invention can be prepared using a method known in the art. That is, the extract of the present invention may be prepared by cutting the raw material into a predetermined size and extracting, filtering, and concentrating with an extracting solvent, or by adding and extracting an extractant, warming it for at least 2 hours, and then filtering and concentrating. As the extraction solvent, water or an alcohol of C 1 to C 5 may be used. Preferably, ethanol may be used as the extraction solvent for preparing the extract of the present invention. The extract prepared by the above method may be prepared in a powder form by vacuum drying.

Most preferably, 50 to 5,000 g of the roots of the plant of Rubus in 50 to 10,000 ml of ethanol are mixed and left for 12 to 72 hours at 22 to 25 ° C. for extraction, filtered and concentrated using a concentrator to remove ethanol. To prepare an ethanol extract or by mixing 50 ~ 5,000g of the roots of the plant of Rubus in distilled water 50 ~ 10,000ml and left for 12-72 hours at 22-25 ℃ to extract and then filtered and concentrated to extract the water extract Can be.

In an embodiment of the present invention, 100 g of bokbunja strawberry roots were added to 80% ethanol, and extracted by standing at 24 ° C. for 24 hours, filtered through a filter paper, and concentrated four times with a concentrator to prepare an ethanol extract of bokbunja strawberry roots (Example 1).

Also, in another embodiment of the present invention, 100 g of bokbunja strawberry root was added to 1000 ml of distilled water, and the mixture was extracted by leaving it at 24 ° C., filtered through a filter paper, and concentrated four times at a temperature of 95 ° C. to prepare a water extract of the raspberry root ( See Example 2).

In addition, in another embodiment of the present invention, 100 g of raspberry root was added to 80% ethanol, left at 24 ° C. for 24 hours, extracted, filtered through a filter paper, and concentrated four times with a concentrator to prepare an ethanol extract of raspberry root (Example 3).

In order to investigate the antimicrobial activity of the ethanol extract, water extract and ethanol extract of raspberry root of bokbunja strawberry root according to the present invention prepared by the above method in the experimental example of Staphylococcus bacteria, Streptococcus bacteria The antimicrobial activity of the root extracts of the Rubus plant against pathogenic fungi, such as Bacillus bacteria, Yersinia bacteria, Propionibacterium, Shigella and Trichophyton, was investigated. 1 and 2). As a result, the extract according to the present invention is Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus intermedius, Staphylococcus sapropiticus, Streptococcus Mutans, Bacillus antirasis, Yersinian pestis, propionibacterium acnes, propionibacterium escidopropionis, It was confirmed that it has antimicrobial activity against Shigella Prexnery and tricophyton Mentagrophytes (see Table 1, Table 2, Figures 1a to 2c).

Therefore, the present invention can provide an antimicrobial composition comprising the root extract of the plant of the genus Rubus. More specifically, the antimicrobial composition may be used to inhibit or kill the growth of pathogenic bacteria and fungi. Preferably, due to pathogenic bacteria skin infections, purulent, otitis media, cystitis, peritonitis, purulent diseases, urinary tract infections, food poisoning, anthrax, plague, caries, acne, methicillin-resistant staphylococcal infections, bacterial dysentery and pathogenic fungi It can be used for the prevention or treatment of athlete's foot.

The composition may comprise the root extract of the plant of the genus Rubus alone or may further comprise one or more pharmacologically acceptable carriers, excipients and diluents. As used herein, `` pharmacologically acceptable '' refers to a composition that is physiologically acceptable and does not normally cause an allergic or similar reaction when administered to a human.

The antimicrobial composition according to the present invention may be formulated using a method known in the art as a powder, granules, tablets, pills, dragees, capsules, solutions, gels, syrups, slurries, suspensions and injections. have.

Furthermore, the root extract of the plant of the genus Rubus according to the present invention can be formulated in various forms. Accordingly, the present invention provides an antimicrobial preparation comprising the root extract of the plant of the genus Rubus. Such formulations include, but are not limited to, cosmetics, disinfectants, sterile detergents and food preservatives.

In the above cosmetics can be easily prepared according to known methods in the art, including one or more excipients and additives generally used in the field of preparation of cosmetic compositions with the root extract of the plant of the genus Lubus of the present invention. More specifically, the cosmetics, such as face lotion, cream, essence, cleansing foam and cleansing water, basic cosmetics such as packs, body oil, etc., color cosmetics such as foundation, lipstick, mascara and makeup base, shampoo, rinse, hair conditioner And hair products such as hair gels, soaps, and the like. Preferably, the cosmetic composition comprising the root extract of the plant of the genus Rubus of the present invention can be used to treat or prevent acne. Therefore, the cosmetic composition of the present invention further comprises a skin exfoliating agent such as plant-derived protease and microorganism-derived protease, such as papain and bromelain, which can improve the therapeutic effect of acne. Can be added. In particular, substances, such as salicylic acid and triclosan, can be added further.

The disinfectant may be prepared by using the root extract of the plant of the genus Rubus of the present invention as it is or by diluting with a dermatologically and pharmacologically acceptable diluent or solvent to an appropriate concentration. The disinfectant may be used on the surface of the organism, preferably on the skin of mammals and most preferably on human skin. The disinfectant may also be used on inanimate surfaces, such as wood, metal, glass, ceramics, plastics, paper and cloth. The disinfectant may be treated on the surface of the living or non-living object using a method including dipping, swab, spraying and brushing. Preferably, the disinfectant may be used in hospitals and homes for the purpose of wound surface, pre-surgical skin disinfection, surgical instrument disinfection, conduit disinfection and the like.

In the case of the sterile detergent, it may be easily prepared according to a method known in the art, including one or more excipients and additives generally used in the preparation of detergents in addition to the root extract of the plant of the genus Lubus according to the present invention. The disinfectant cleaner may be used for kitchen or food use, and the disinfectant cleaner may further include anionic surfactants, nonionic surfactants, amphoteric surfactants, and the like, in addition to the root extract of plants of the genus Rubus. Examples of anionic surfactants include alkylbenzenesulfonates, alphaolefin sulfates, sodium or ammonium lauryl ether sulfates, and examples of nonionic surfactants include fatty acid amides, alkylpolyglucosides, ethoxylated fatty alcohols, and ethoxylations. Nonyl phenol type etc. are mentioned. Examples of amphoteric surfactants include betaine-based surfactants, amine oxides, and the like. In addition to the above surfactants, diluents such as flavors, pigments and water may be optionally added.

In the case of a food disinfectant detergent, it may be prepared by further adding a food acceptable solvent or diluent to the root extract of the plant of the genus Lubus according to the present invention and diluting to an appropriate concentration. The food sterilizing detergent may be used for sterilization and washing of fruits, fish and meat, for example.

Food preservatives may be prepared by further adding a food acceptable solvent or diluent to the root extract of the plant of the genus Rubus according to the present invention for the purpose of enhancing the preservation of foods. The food preservative of the present invention may be added together with the raw materials in the manufacturing process of the food or prepared into a separate water-soluble suspension. In addition, the food preservative according to the present invention may be used to immerse the target food or spray the food preservative of the present invention onto the target food.

Hereinafter, the specific method of the present invention will be described in detail with reference to Examples, but the scope of the present invention is not limited only to these Examples.

<Example 1>

Bokbunja Strawberry ( Rubus coreanus ) Ethanol Extract of Roots

After drying in 1000ml of 80% ethanol, 100g of bokbunja strawberry root (Gyeongdong Market medicinal herb), which was chopped in the form of medicinal herbs, was mixed and extracted by standing at 24 ° C for 24 hours. The extract was then separated into sludge and solution using Whatman filter paper. To remove the ethanol from the separated solution it was concentrated 4 times at 38 ℃ using a concentrator (Evaporator, Heidolph vv 2000).

<Example 2>

Bokbunja Strawberry ( Rubus coreanus ) Preparation of Root Extract

100 g of bokbunja strawberry roots were mixed with 1000 ml of first distilled water, and then extracted by standing at 24 ° C. for 24 hours. The extract was then separated into sludge and solution using Whatman filter paper. The separated solution was concentrated four times by boiling water at a temperature of 95 ℃.

Experimental Example 1

Bokbunja strawberry according to the present invention ( Rubus coreanus ) Antimicrobial Activity of Root Extracts

The antimicrobial activity of the bokbunja strawberry root extract prepared in Examples 1 and 2 was measured. As the strains used, various pathogenic bacteria such as Staphylococcus bacteria, Streptococcus bacteria, Bacillus bacteria, Yersinia bacteria, Propionibacterium bacteria and Shigella genus were distributed from known deposit institutions. (Table 1).

A single colony of each test strain was inoculated into a 200 ml flask containing 10 ml of Nutrient broth and incubated for 12 hours with stirring at 37 ° C. The culture was passaged for 7 hours by inoculating an initial OD of 0.1 in a 200 ml flask containing 10 ml of nutrient medium. Each test strain culture activated in the above manner was adjusted to 2 × 10 8 in the number of bacteria per plate, and then poured into 1000mm Petri dishes with 13 ml of nutrient agar medium at 60 ° C and hardened well. Then, a 10 mm paper disk (Toyo Roshi Kaisha, Ltd, Japan), dried for 24 hours in a 56 ° C. incubator, completely dehumidified, was placed on the plate, and then the ethanol extract of the bokbunja strawberry root of Example 1 on each disk or 100 μl each of the water extract of the bokbunja strawberry root of Example 2 was injected. The plate was incubated for 12 hours in a 37 ° C. incubator, and then the growth stop of the resulting bacteria, namely, the clear zone, was determined, and the diameter of the plate was measured using a veneer calipers.

As a result, it was confirmed that the ethanol extract of bokbunja strawberry root has an antimicrobial effect in all the test strains. In particular, the antibacterial effect of the ethanol extract of the bokbunja strawberry root was the highest against the acne bacterium Propionibacterium acnes , followed by the pest pest Yersinia pestis The antimicrobial activity against was high. In addition, the antimicrobial activity of the ethanol extract of the bokbunja strawberry root of the present invention was high for the cavities Streptococcus mutans , Bacillus anthracis and the heterozygo Shigella flexneri . . In particular, the ethanol extract of bokbunja strawberry root according to the present invention was shown to have antibacterial activity against all four anthrax strains. In addition, the ethanol extract of the bokbunja strawberry root has been shown to have an antimicrobial activity against bacteria and methicillin resistant strains of Staphylococcus which is a cause of purulent disease, urinary tract infection, skin disease, food poisoning. On the other hand, the water extract of bokbunja strawberry root was found to have antibacterial activity against bacteria and anthrax in the Staphylococcus (Table 1 and Figures 1a to 1c).

Antimicrobial Activity of Bokbunja Strawberry Root Extract against Various Pathogenic Bacteria Test microorganism Gram Dyeing Strain number Where to get Transparent ring diameter (mm) Ethanol extract Water extract One Staphylococcus aureus + KCTC 1621 Korea KCTC 16 - 2 Staphylococcus aureus skin isolate + CAU-HY90 Chung-Ang Medical School 28 - 3 Staphylococcus aureus methicillin resistant strain + ChDC Sa1 Chosun University 17 - 4 Staphylococcus aureus methicillin resistant strain + ChDC Sa2 Chosun University 18 - 5 Staphylococcus aureus methicillin resistant strain + ChDC Sa3 Chosun University 13 - 6 Staphylococcus epidermis + KCTC 1917 Korea KCTC 16 11.5 7 Staphylococcus epidermidis skin isolate + CAU-HY19 Korea KCTC 20 - 8 Staphylococcus intermedius + KCTC 3344 Korea KCTC 17 11.5 9 Staphylococcus sapropitus + KCTC 3345 Korea KCTC 18 - 10 Streptococcus mutans + ATCC 25175 US ATCC 30 - 11 Bacillus anthracis + ATCC 14578 US ATCC 21 - 12 Bacillus anthracis + ATCC 14185 US ATCC 24 11.5 13 Bacillus anthracis + ATCC 14186 US ATCC 24 12 14 Bacillus anthracis + Pasteur No.2 Army US Army Medical Research Institute 21 - 15 Yersinian Festival + NCTC 5923 UK NCTC 39 - 16 Propionibacterium Acnes + B715 Chosun University 40 - 17 Shigella Prexnery - KCTC 2008 Korea KCTC 19

<Example 3>

raspberry( Rubus crataeifolius ) Ethanol Extract of Roots

100 g of raspberry root (obtained from Hongcheon-gun, Yacheon-gun, Gangwon-do) was dried in 1000 ml of 80% ethanol and mixed with 100 g of ethanol to prepare an ethanol extract of the raspberry root in the same manner as in Example 1.

Experimental Example 2

Raspberries according to the present invention ( Rubus crataeifolius ) Antimicrobial Activity of Root Extracts

The antimicrobial activity of the ethanol extract of raspberry roots prepared in Example 3 was measured. Examples of strains used include trichophytons and various pathogenic bacteria, such as Staphylococcus bacteria, Streptococcus bacteria, Bacillus bacteria, Yersinia bacteria, Propionibacterium and Shigella bacteria, which are distributed from known deposit institutions. Mold on the inside It was used (Table 2), and the antimicrobial activity was measured by the same method as Experimental Example 1.

As a result of the experiment, the ethanol extract of raspberry root was confirmed to have an antimicrobial effect in all test strains in the same manner as the ethanol extract of bokbunja strawberry root prepared in Example 1, and the antimicrobial activity against tricophyton mentagrophytes, which is athlete's foot Was found (Table 2 and FIGS. 2A-2C).

Antimicrobial Activity of Raspberry Root Extract against Various Pathogenic Bacteria and Fungi Test microorganism Gram Dyeing Strain number Where to get Transparent ring diameter (mm) Ethanol Extract One Staphylococcus aureus + KCTC 1621 Korea KCTC 23 2 Staphylococcus aureus skin isolate + CAU-HY90 Chung-Ang Medical School 19.5 3 Staphylococcus aureus methicillin resistant strain + ChDC Sa1 Chosun University 24 4 Staphylococcus aureus methicillin resistant strain + ChDC Sa2 Chosun University 16 5 Staphylococcus aureus methicillin resistant strain + ChDC Sa3 Chosun University 23 6 Staphylococcus epidermis + KCTC1917 Korea KCTC 23 7 Staphylococcus epidermidis skin isolate + CAU-HY19 Korea KCTC 25 8 Staphylococcus intermedius + KCTC 3344 Korea KCTC 25 9 Staphylococcus sapropitus + KCTC 3345 Korea KCTC 24 10 Streptococcus mutans + ATCC 25175 US ATCC 32 11 Bacillus anthracis + ATCC 14578 US ATCC 20 12 Bacillus anthracis + ATCC 14185 US ATCC 22 13 Bacillus anthracis + ATCC 14186 US ATCC 23 14 Bacillus anthracis + Pasteur No.2 Army US Army Medical Research Institute 25 15 Yersinian Festival + NCTC 5923 UK NCTC 34 16 Propionibacterium Acnes + B715 Chosun University 42 17 Propionibacterium Acnes + KCTC 3314 Korea KCTC 37 18 Propionibacterium Ecido Propionis + KCTC 5020 Korea KCTC 39 19 Shigella Prexnery - KCTC 2008 Korea KCTC 19 20 Tricophyton Mentagrophytes                                              + KCTC 6077 Korea KCTC 24

<Manufacture example 1>

Preparation of skin cleanser comprising root extract of bokbunja strawberry or raspberry according to the present invention

Root extract of raspberry or raspberry of Bokbunja strawberry prepared in Examples 1 to 3 in 12 g of glycerin 6 g, monoalkyl phosphate 2.0 g, sodium hydroxide aqueous solution 0.5 g, myristic acid 1.5 g, and a fragrance trace amount 0.5 wt% of the extract was mixed, stirred in a homo mixer, heated at 60 ° C. for 3 minutes, degassed, and cooled to 37 ° C. to prepare a face wash composition.

<Manufacture example 2>

Preparation of a soap comprising the root extract of bokbunja strawberry or raspberry according to the present invention

Soap maker 99.5% by weight (including water) and 0.5% by weight of the root extract of bokbunja strawberry or root extract of wild strawberry prepared in Examples 1 to 3 prepared in Example 1 in a mixer Extruded, cut and molded to prepare a solid soap composition.

<Manufacture example 3>

Preparation of a kitchen detergent comprising the root extract of bokbunja strawberry or raspberry according to the present invention

20% by weight alkylethersulfonate, 4% by weight alkylpolyglucoside, 4% by weight lauryldimethylamine oxide, 7% by weight Cocamidopropyl Betaine, ethoxy Detergent by adding 5% by weight of lauryl alcohol, 0.3% by weight of EDTA, a small amount of pigment, 0.5% by weight of flavor, root extract of bokbunja strawberry or root extract of wild strawberry prepared in Examples 1 to 3 and the remaining amount of purified water Was prepared.

<Manufacture example 4>

Preparation of a tablet comprising the root extract of bokbunja strawberry or raspberry according to the present invention

250 mg of Root Extract of Raspberry or Raspberry of Bokbunja Strawberry prepared in Examples 1 to 3, 260 mg of lactose for excipients and 35 mg of Avicel (microcrystalline cellulose), 15 mg of sodium starch glyconate as a disintegration aid, L for Jitata as a binder -HPC (Low-hydroxyprophylcellulose) with 80mg mixed in a U-type mixer for 20 minutes. After mixing was completed, 10 mg of magnesium stearate was further added as a lubricant and mixed for 3 minutes. Tablets were prepared by tableting and film coating after a quantitative test and a humidity test.

Production Example 5

Preparation of syrup comprising root extract of bokbunja strawberry or wild strawberry according to the present invention

Dissolve an appropriate amount of sucrose in a certain amount of water, add 80 mg of paraoxymethyl benzoate and 16 mg of paraoxypropyl benzoate as a preservative, and extract the root extract of raspberry or raspberry of Bokbunja strawberry prepared in Examples 1 to 3. 4.5 g of each was added and maintained at 60 ° C., completely dissolved, cooled, and 150 ml of distilled water was added to prepare 3% syrup.

<Manufacture example 6>

Preparation of a capsule comprising a root extract of bokbunja strawberry or raspberry according to the present invention

A capsule was prepared by filling a hard gelatin capsule with 450 mg of the root extract of raspberry or wild strawberry root extract prepared in Examples 1 to 3 mixed with 50 mg of lactose.

Root extract of the plant of the genus Lubus according to the present invention is antibacterial against skin diseases, purulent diseases, surgical wound infections, urinary tract-inducing bacteria and food poisoning bacteria, anthrax, dysentery bacteria, pest bacteria, caries bacteria, acne bacteria, methicillin-resistant staphylococcus bacteria and athlete's foot fungi. Because it has activity, purulent diseases such as skin infections, purulent, otitis media, cystitis, peritonitis, urinary tract infections, food poisoning, anthrax, plague, caries, acne, methicillin-resistant staphylococcal infections caused by pathogenic bacteria. It can be very useful for the prevention or treatment of athlete's foot caused by bacterial dysentery and pathogenic fungi. In addition, the root extract of the plant of the genus Rubus of the present invention can be prepared in the form of cosmetics, disinfectants, sterile detergents and food preservatives.

Claims (12)

  1. Extracted with water or an alcohol of C 1 ~C 5 bokbunja strawberry (Rubus coreanus) or raspberry (Rubus crataegifolius) antimicrobial composition comprising a root extract of a plant.
  2. delete
  3. According to claim 1, wherein the composition is Staphylococcus sp., Streptococcus sp., Bacillus sp., Yersinia sp., Propionibacterium A composition characterized by exhibiting antimicrobial activity against fungi selected from the group consisting of the genus Propionibacterium sp. And Shigella sp.
  4. According to claim 3, wherein the bacteria of the genus Staphylococcus are Staphylococcus aureus , Staphylococcus epidermidis , Staphylococcus intermedius and S. aureus Philococcus saprophyticus ( S. saprophyticus ), characterized in that the composition selected from the group consisting of.
  5. The composition of claim 3, wherein the Streptococcus bacteria are Streptococcus mutans .
  6. The composition of claim 3, wherein the bacterium is Bacillus anthracis .
  7. The composition of claim 3, wherein the genus Bacteria is Yersinia pestis .
  8. The composition of claim 3, wherein the bacteria of the genus Propionibacterium are Propionibacterium acnes or Propionibacterium ecidopropionici .
  9. The composition of claim 3, wherein the genus Shigella is Shigella flexneri .
  10. 4. The composition of claim 3, wherein the genus Tricophyton fungus is Trichophyton mentagrophytes .
  11. delete
  12. Antimicrobial preparations containing the root extract of Bokbunja strawberry ( Rubus coreanus ) or Rubus crataegifolius plant.
KR1020050005059A 2005-01-19 2005-01-19 Antimicrobial composition containing the root extract of R. coreanus MIQ or R. crataegifolius KR100699333B1 (en)

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KR101348102B1 (en) 2012-11-12 2014-01-07 손기정 A composition comprising extracts of herbal mixture for treating or preventing interstitial cystitis
KR20140025239A (en) * 2012-08-22 2014-03-04 유씨엘 주식회사 Cosmetic composition containing active fractions of rubus coreanus miq root
KR20180060430A (en) 2016-11-29 2018-06-07 서울대학교산학협력단 Antibacterial active composition of strawberry leaf extract and method for controlling anthrax using the same
KR101929115B1 (en) * 2017-03-13 2018-12-13 국민대학교산학협력단 Composition for Preventing Biofilm Formation Containing Extract of Licorice and Extract of Rasberry
KR101929565B1 (en) * 2017-03-13 2018-12-14 국민대학교산학협력단 Composition for Preventing Biofilm Formation Containing Extract of Raspberry
KR20190099782A (en) * 2018-02-20 2019-08-28 충남대학교산학협력단 Antimicrobial composition against pathogenic bacteria containing Fragaria ananassa extract or its fraction as effective component

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KR101012687B1 (en) * 2010-12-09 2011-02-09 김정숙 Manufacturing method of the body rinse using natural material
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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20140025239A (en) * 2012-08-22 2014-03-04 유씨엘 주식회사 Cosmetic composition containing active fractions of rubus coreanus miq root
KR102062854B1 (en) * 2012-08-22 2020-01-07 유씨엘 주식회사 Cosmetic composition containing active fractions of Rubus coreanus MIQ root
KR101348102B1 (en) 2012-11-12 2014-01-07 손기정 A composition comprising extracts of herbal mixture for treating or preventing interstitial cystitis
KR20180060430A (en) 2016-11-29 2018-06-07 서울대학교산학협력단 Antibacterial active composition of strawberry leaf extract and method for controlling anthrax using the same
KR101929115B1 (en) * 2017-03-13 2018-12-13 국민대학교산학협력단 Composition for Preventing Biofilm Formation Containing Extract of Licorice and Extract of Rasberry
KR101929565B1 (en) * 2017-03-13 2018-12-14 국민대학교산학협력단 Composition for Preventing Biofilm Formation Containing Extract of Raspberry
KR20190099782A (en) * 2018-02-20 2019-08-28 충남대학교산학협력단 Antimicrobial composition against pathogenic bacteria containing Fragaria ananassa extract or its fraction as effective component
KR102050305B1 (en) 2018-02-20 2019-11-29 충남대학교 산학협력단 Antimicrobial composition against pathogenic bacteria containing Fragaria ananassa extract or its fraction as effective component

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