KR20030015654A - Skin external compositions containing raspberry leaf or fruit extract for reducing irritation and inflammation in skin - Google Patents

Abstract

PURPOSE: Provided is a composition containing an extract of leaves or fruits of raspberry inhibiting IL-6, IL-8 and PGE2 being involved in skin irritation and inflammation production. Therefore, the composition can be used as a cosmetic composition having an effect for preventing skin irritation caused by UV light or cosmetic. CONSTITUTION: The skin composition for external application contains 0.00001 to 30% by weight of an extract of leaves or fruits of raspberry such as Rubus coreanum, Rubus idaeus and Rubus parvifolius. The composition inhibits IL-6, IL-8 and PGE2 by substance P and bradykinin, and consequently prevents skin irritation and inflammation.

Description

Skin external compositions containing raspberry leaf or fruit extract for reducing irritation and inflammation in skin}

The present invention relates to an external composition for skin having an effect of alleviating skin irritation and inflammation, and more particularly, consisting of raspberry (Rubus coreanum), Rubberry (Rubus idaeus), and Rubberberry (Rubus parvifolius) Prostaglandin-E2 by UV and Biosynthesis of IL-6 and IL-8 by Substance P or Bradykinin in Skin Cells by Containing Leaf or Fruit Extracts of One or More Plants Selected from the Group The present invention relates to an external preparation composition for skin that exhibits anti-inflammatory effect and skin irritation-reducing effect through inhibiting biosynthesis of (PGE2).

Skin is a part of the body that is directly exposed to the external environment, and when exposed to excessive ultraviolet rays or contaminants, skin irritation and inflammatory reactions such as erythema, edema, and itching are induced. Skin troubles caused by such stress sources are not only aesthetic problems, but the substances produced in the inflammatory process are known to cause pigmentation of the skin and promote the collapse of skin elastic fibers, thereby affecting the increase of skin wrinkles. have.

On the other hand, in modern society, many people, both young and old, use cosmetics. In some sensitive skin, the use of cosmetics may cause skin irritation and inflammation. In particular, retinoids or fruit acids used as raw materials for functional products for the purpose of exfoliation, skin wrinkle improvement and prevention, and surfactants added for formulation are the main substances causing troubles on the skin. There have been many limitations to the use of substances in concentrations that produce the maximum efficacy.

Therefore, in order to reduce skin irritation and inflammation caused by various stress sources, and to reduce side effects due to the use of cosmetics, it is urgently required to develop substances having anti-inflammatory and skin irritation efficacy.

Therefore, researches on how to reduce the skin irritation and the generation of inflammation are actively progressed. Previous studies have shown that various cytokinins in skin cells and immune cells, such as neuropeptides such as substance P secreted from nerve cells or kinins such as bradykinin activated by stress in tissues or plasma, can be produced. It is known that inflammation is formed by the secretion of (cytokine; in particular, IL-6 and IL-8) and PGE2, and accordingly, neuropeptide antagonists such as substance P, bradykinin antagonists, etc. have been proposed, There was no effect.

At this point we also effectively control skin irritation caused by UV and other environmental stresses that have a huge impact on skin irritation and inflammation production, to relieve skin irritation and to prevent or mitigate the formation of inflammation. We studied to find a raw material that modulates IL-6, IL-8 and PGE2 (prostaglandins produced by COX-2), which are increased by various stimulation mediators. As a result, 300 kinds of raw materials were searched for use as active ingredients. As a result, raspberry fruit and leaf extracts inhibit all of IL-6, IL-8 and PGE2 involved in skin irritation and inflammation. The present invention has been found to be effective in relieving skin irritation caused by retinol, lactic acid, SLS, and the like contained in cosmetics.

Accordingly, it is an object of the present invention to provide an external composition for skin having an excellent effect of improving skin irritation or preventing or alleviating skin inflammation.

In order to achieve the above object, the composition for external application for skin according to the present invention is selected from raspberry (Rubus coreanum), Rubberry (Rubus idaeus) and Rubber parsley (Rubus parvifolius), which is a kind of raspberry, together with other compositions formulated in the external preparation. It is characterized by containing the leaf or fruit extract of at least one plant in an amount of 0.00001 to 30% by weight.

Hereinafter, the present invention will be described in more detail.

Raspberry is a rosaceae, and among the plants belonging to it, in the present invention, bokbunja, bruises and daisies were used.

The present invention has found that the leaves or fruit extracts of these plants have the effect of antagonizing the action of bradykinin and substance P related to the induction of skin inflammation such as pain, erythema and itching. It has been found to inhibit the secretion of inflammatory mediators IL-6 and IL-8 secreted by bradykinin and substance P in cells and glial cell lines, and to inhibit the biosynthesis of PGE2, an inflammatory mediator increased by UV light. .

Retinol, which is used as a functional cosmetic raw material, also has side effects such as erythema, blister formation, edema, and itching on the skin, and the extracts effectively inhibit the biosynthesis of MCP-1, an inflammation mediator of retinol in skin cells. In animal models and human subjects, it was confirmed that retinol inhibits skin erythema. Moreover, raspberry extracts have the effect of relieving skin irritation caused by sodium lauryl sulfate (SLS) added to cosmetics as a surfactant and lactic acid, a functional ingredient, and skin induced by UV irradiation in clinical trials conducted on volunteers. It has been shown to inhibit erythema.

Therefore, it can be seen that the above extracts of Bokbunja, Blackberry and Blackberry have an effect by blocking the production of factors involved in skin irritation and inflammation.

The natural product extract used in the present invention is not limited to the terminology of the plant itself, and the leaching liquid obtained by leaching or leaching from the herbal medicine, or the concentrate obtained by partially or completely concentrating the leaching liquid, or drying the concentrate again. It contains the chemicals that have the main effect by being contained in the prepared by distillation, premises, regular, flow extract (hereinafter referred to as extract extract) and natural products.

The external preparation composition for skin of the present invention contains leaf or fruit extracts of at least one plant selected from the above bokbunja, blackberry and blackberry in an amount of 0.00001-30% by weight, preferably 0.001-10% by weight. If the content is less than 0.00001% by weight, it is difficult to obtain the desired effect in the present invention, and if it exceeds 30% by weight, there is a problem in formulation stability.

The external preparation composition of the present invention is used for the purpose of preventing or alleviating skin irritation and inflammation, and is not particularly limited in the formulation, for example, softening longevity, nourishing longevity, massage cream, nutrition cream, pack, gel Or a cosmetic composition having a formulation of a skin-adhesive cosmetic, and may also be a transdermal dosage form such as a lotion, ointment, gel, cream, patch or spray.

In addition, in each dosage form, components other than the essential components mentioned above can be suitably selected and blended by those skilled in the art without difficulty according to the kind or purpose of use of other external preparations.

Hereinafter, the configuration and effect of the present invention will be described in more detail with reference to Examples and Test Examples, but the present invention is not limited only to these examples.

Reference Example 1 Preparation of Bokbunja Extract

1 kg of bokbunja leaves and berries were washed with purified water and dried. The powdered bokbunja powder was placed in 5 l of propylene glycol, extracted in an extractor equipped with a cooling condenser for 3 days, and filtered twice using Whatman filter paper. . The solvent was then completely concentrated after evaporation using a distillation apparatus equipped with a cooling condenser.

REFERENCE EXAMPLE 2 Preparation of Waxberry Extract

Extracted by the same method as in Reference Example 1, using the leaves and fruit 1kg brussels.

REFERENCE EXAMPLE 3 Preparation of Dabberberry Extract

It was extracted in the same manner as in Reference Example 1 using 1kg of bilberry leaves and fruit.

[Test Example 1] Evaluation of the inhibitory effect of IL-6 biosynthesis by substance P stimulation in glial cell line, U373 MG of raspberry extract

Human glial cell line U373 MG was added to each well of a 96-well test plate with MEM (Minimum Essential Media, GIBCO BRL, Life Technologues) medium containing 10% fetal bovine serum (FBS). ^Four cells were attached and incubated for 24 hours. After removing the medium, washed once with 200 μl of phosphate buffered solution (phosphate buffered saline (PBS), and the extracts of Reference Examples 1 to 3 to be evaluated in 200 μl of MEM medium without FBS concentration shown in Table 1 below. As many treatments as incubated for 5 hours. 25 μl of the culture was taken to measure the effect of inhibiting IL-6 release by substance P through IL-6 ELISA (Pharmingen, OptEIA Human IL-6 set). The results are shown in Table 1 below.

ingredient Extract concentration (ppm) IL-6 (pg / 10 ⁵ cells) % Inhibition Rate * (-) Substance P + Vehicle 10.5 ± 32 - (+) SP (1μM) + Vehicle 981.4 ± 61 - (+) SP (1μM) + bokbunja extract (Reference Example 1) 100 ppm 505.7 ± 41 48.5 (+) SP (1μM) + Beeberry Extract (Reference Example 2) 100 ppm 602.7 ± 38 39.6 (+) SP (1μM) + Dubberry Strawberries Extract (Reference Example 3) 100 ppm 583.0 ± 21 41.0 * Calculated based on the difference in IL-6 production between U373 cells not treated with Substance P and U373 cells treated with Substance P.

From Table 1, it can be seen that the bokbunja extract, Brussels berry extract and Burberry strawberry extract has the effect of inhibiting the biosynthesis of IL-6, an inflammation mediator by substance P stimulation in human glial cells U373.

[Test Example 2] Evaluation of IL-6 and IL-8 Biosynthesis Inhibitory Effect of Raspberry Extract by Bradykinin Stimulation in Dermal Fibroblasts

Cultured fibroblasts were isolated from human epidermal tissue and 2 × 10 ⁴ cells were placed on 96-well test plates with DMEM (Dulbecco's Modified Eagle's Media, GIBCO BRL, Life Technologues) medium containing 10% FBS. Attached to each well and incubated for 24 hours. After culturing the cells for 24 hours with DMEM without FBS, the extracts of Reference Examples 1 to 3 to be evaluated by DMEM containing 1% fetal bovine serum were treated by the concentrations shown in Table 2 below, followed by 5 hours. After incubation, the culture medium was taken, and 25 μl of each culture solution was subjected to ELISA (using Pharmingen, OptEIA Human IL-6 / IL-8 set) for IL-6 and IL-8. 6 and IL-8 was evaluated for the biosynthesis inhibitory ability. The results are shown in Table 2 below.

ingredient Extract concentration (ppm) IL-6 (pg / 10 ⁵ cells) IL-8 (pg / 10 ⁵ cells) IL-6 Inhibition Rate * IL-8 Inhibition Rate * (-) Bradykinin + Vehicle 0.86 ± 0.27 2.93 ± 0.99 - - (+) BK (1μM) + Vehicle 2.63 ± 0.32 6.71 ± 1.37 - - (+) BK (1 μM) + bokbunja extract (Reference Example 1) 10 ppm 1.55 ± 0.11 5.63 ± 0.05 61.0 28.5 (+) BK (1 μM) + bokbunja extract (Reference Example 1) 50 ppm 1.48 ± 0.05 5.31 ± 0.57 65.0 37.0 (+) BK (1 μM) + Beeberry Extract (Reference Example 2) 10 ppm 1.60 ± 0.27 5.79 ± 0.27 58.2 24.3 (+) BK (1μM) + Dubberry Extract (Reference Example 3) 10 ppm 1.59 ± 0.74 5.34 ± 0.19 58.8 36.2 * Calculation is based on the difference in IL-6 or IL-8 production between the dermal fibroblasts treated with Bradykinin and the dermal fibroblasts treated with Bradykinin

From Table 2, it can be seen that the bokbunja extract, Brussels berry extract and Burberry strawberry extract has the inhibitory effect of biosynthesis of IL-6 and IL-8, which are inflammation mediators by bradykinin stimulation in skin fibroblasts.

[Test Example 3] Evaluation of the inhibitory effect of prostaglandin biosynthesis by UV stimulation on keratinocytes of raspberry extract

Keratinocytes isolated from human epidermal tissue (5 × 10 ⁴ ) were placed in 24-well test plates and attached for 24 hours. A FBS is replaced with a non-containing medium, and incubated for 18 hours and then was treated with aspirin (aspirin) in the keratinocytes of a final concentration of 500 μ M, prostaglandin biosynthetic enzymes present in keratinocytes (prostaglandin H2 synthetase, or cyclooxygenase, COX) was removed. After 2 hours of aspirin treatment, each well containing keratinocytes was washed twice with PBS, and 100 μl of PBS was added to each well. The keratinocytes were irradiated with UV 30mJ / cm 2 using an ultraviolet B lamp (Model: F15T8, UV B 15W, Sankyo Dennki, Japan), and then PBS was removed and each cell was cultured with keratinocyte culture ( keratinocyte growth media, Clonetics, BioWhittacker, MD, USA) 250 μl was added. The extracts of Reference Examples 1 to 3 to be evaluated here were treated by the concentrations shown in Table 3 below, followed by incubation for 16 hours. By taking an appropriate amount of the culture supernatant and quantifying biosynthesized PGE2 for 16 hours, the prostaglandin inhibitory effect of the raspberry extract was determined. The amount of PGE2 was quantified using an enzyme immunoassay, and the results are shown in Table 3 below.

Composition Extract concentration (ppm) PGE ₂ (pg / 10 ⁵ cells) % Inhibition Rate * (-) UV B + Vehicle 37.7 ± 9.2 - (+) UV B + Vehicle 287.5 ± 19.5 - (+) UV B + bokbunja extract (Reference Example 1) 10 ppm 80.4 ± 11.4 82.9 (+) UV B + bokbunja extract (Reference Example 1) 1 ppm 233.7 ± 41.2 21.5 (+) UV B + Beetroot Extract (Reference Example 2) 10 ppm 111.6 ± 9.7 70.4 (+) UV B + bilberry extract (Reference Example 3) 10 ppm 196.3 ± 28.1 36.5 * Calculation is based on the difference between PGE ₂ production amount of keratinocytes not irradiated with ultraviolet rays and keratinocytes irradiated with ultraviolet rays.

From Table 3, it can be seen that the Bokbunja extract, Brussels berry extract, and Brussels berry extract effectively inhibit the production of prostaglandins by UV at a concentration of 10 ppm. In particular, since the produced PGE2 is known to be mainly produced by the COX-2 enzyme, the test shows that each extract inhibits the induction or activity of the COX-2 enzyme.

Test Example 4 Evaluation of MCP-1 Biosynthesis Inhibitory Effect of Raspberry Extract by Retinol Stimulation in Dermal Fibroblasts

Cultured fibroblasts isolated from human epidermal tissue were cultured in D 96 (Dulbecco's Modified Eagle's Media, GIBCO BRL, LifeTechnologues, Inc.) containing 10% FBS, 2 × 10 ⁴ each in 96-well test plates. Attach to wells and incubate for 24 hours. After culturing the cells for 24 hours with DMEM without FBS, the extracts of Reference Examples 1 to 3 to be evaluated by DMEM containing 1% fetal bovine serum were treated by the concentrations shown in Table 4 below, followed by 5 hours. After incubation, the culture medium was taken, and ELISA (Pharmingen, OptEIA Human MCP-1 set) on MCP-1 was performed on 25 μl of each culture, to evaluate the biosynthesis inhibition of MCP-1 by retinol. The results are shown in Table 4 below.

Composition Extract concentration (ppm) MCP-1 (ng / 10 ⁵ cells) % Inhibition Rate * (-) Retinoic acid + Vehicle 2.4 ± 0.34 - (+) RA (10μM) + Vehicle 13.2 ± 1.14 - (+) RA (10 μM) + bokbunja extract (Reference Example 1) 10 ppm 8.7 ± 1.16 41.7 (+) RA (10 μM) + bokbunja extract (Reference Example 1) 50 ppm 7.4 ± 0.72 53.7 (+) RA (10μM) + Beeberry Extract (Reference Example 2) 10 ppm 10.9 ± 0.31 21.3 (+) RA (10μM) + Dumberberry Extract (Reference Example 3) 10 ppm 8.4 ± 1.01 44.4 * Calculation is based on the difference in MCP-1 production between skin fibroblasts not treated with retinoic acid and skin fibroblasts treated with Retinoic acid.

Test Example 5 Evaluation of Retinol Erythema Stimulation Efficacy Efficacy of Raspberry Extract by Animal Test

The composition shown in Table 6 was applied to the back skin of the New Zealand white rabbit eight times a day for two days at intervals. After application of the substance, the cumulative erythema and the skin formation score and the edema formation score were accumulated to determine the cumulative skin irritation index. The cumulative skin irritation index was evaluated according to the criteria shown in Table 5, and the results are shown in Table 6 below. The stimulus index shown in the results is based on the calculation method of commonly used Primary Irritation Index (Draize, J.H., Appraisal of the safety of chemical in foods, drugs and cosmetics).

Degree of skin irritation grade 1) No erythema and no erythema formation Very erythema (only visible to the naked eye) Clear erythema Strong and moderate erythema Deep erythema Strong erythema and crust formation 01234 2) No edema forming edema Very mild edema (only visible to the naked eye) Clear edema (distinguishable from clear) Moderate edema (if swollen up to 1 mm) Strong edema (swelling more than 1 mm and exposed area) Extended out) 01234 Primary irritation index (P.I.I.) = (Average of sum of erythema and edema ratings) / 4

Composition Extract concentration (ppm) Stimulation Relief (%) * (-) Retinol + Vehicle - - Retinol 3000 IU + Vihicle - - (+) Retinol 3000 IU + bokbunja extract (Reference Example 1) 100 ppm 25.9 (+) Retinol 3000 IU + Beetroot Extract (Reference Example 2) 100 ppm 38.5 (+) Retinol 3000 IU + Skyberry Extract (Reference Example 3) 100 ppm 32.1 * Calculation of stimulation relaxation rate based on the difference between cumulative stimulation index between the group not coated with retinol and the group coated

From Table 6, it can be seen that the bokbunja, brussels and locust berries extracts are effective in relieving skin irritation caused by retinol.

Test Example 6 Evaluation of Efficacy of Skin Irritant by Lactic Acid and SLS of Raspberry Extract-Containing Formulation through Animal Test

The New Zealand white rabbit's back skin was applied to the composition formulated as an emulsion (emulsion), as shown in Table 7 below, for a total of eight times for four days at twice daily intervals. After application of the material, the cumulative stimulation index and edema formation score values were accumulated based on the criteria of Table 5, and the cumulative irritation index was calculated. It is shown in Table 8 below.

ingredient Content (% by weight) Comparative Example 1 Example 1 Example 2 Example 3 Example 4 Example 5 Example 6 Bokbunja Extract (Reference Example 1) - 1.00 - - 1.00 1.00 1.00 Blackberry Extract (Reference Example 2) - - 1.00 - 1.00 - 1.00 Fruit Strawberry Extract (Reference Example 3) - - - 1.00 - 1.00 1.00 Lactic acid 20.00 20.00 20.00 20.00 20.00 20.00 20.00 SLS 0.30 0.30 0.30 0.30 0.30 0.30 0.30 Beeswax 4.00 4.00 4.00 4.00 4.00 4.00 4.00 Polysorbate 1.50 1.50 1.50 1.50 1.50 1.50 1.50 Sorbitan sesquioleate 0.70 0.70 0.70 0.70 0.70 0.70 0.70 Liquid paraffin 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Caprylic / Capric Triglycerides 5.00 5.00 5.00 5.00 5.00 5.00 5.00 glycerin 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Butylene glycol 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Propylene glycol 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Carboxy Vinyl Polymer 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Triethanolamine 0.20 0.20 0.20 0.20 0.20 0.20 0.20 antiseptic Quantity Quantity Quantity Quantity Quantity Quantity Quantity Pigment Quantity Quantity Quantity Quantity Quantity Quantity Quantity Spices Quantity Quantity Quantity Quantity Quantity Quantity Quantity Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100

Composition Stimulation Relief (%) * Example 1 40.5 Example 2 15.5 Example 3 29.8 Example 4 41.9 Example 5 35.1 Example 6 39.4 Comparative Example 1 -

From Table 8, it was confirmed that the cosmetic containing at least one extract of Bokbunja, Blackberry and Blackberry extract of the present invention has an effect of alleviating skin irritation caused by lactic acid and SLS.

Test Example 7 Evaluation of Efficacy of Skin Erythema by Retinol in Raspberry Extract-Containing Formulations by Human Test

A guideline for evaluating the safety of the Cosmetic Toiletry and Fragrance Association (CTFA) was to evaluate the effect of retinol-induced skin erythema-induced effects of raspberry extracts on healthy volunteers who had not taken nonsteroidal anti-inflammatory drugs and other steroid preparations for a month before entering the trial. (Safety Testing Guideline, 1981, p3). That is, the composition formulated with the emulsion as prescribed in Table 10 below in the forearm of 10 subjects was continuously closed patch for 24 hours. The number of patches was performed two to four times in consideration of the response level and skin condition of the subject, and the test was performed one hour after the removal of the patch each time, and the last test was performed up to eight days from the date of patch application. Evaluation was continued even after the end of the patch to reflect the degree of recovery of stimulation, and no score was given when erythema completely disappeared and only pigmentation remained. At each test point, the degree of stimulation was evaluated according to the determination method shown in Table 9 below, and the stimulation relaxation rate was calculated from Equation 1 from the sum and the results are shown in Table 11 below.

Degree of irritation of skin grade Mild erythema with intense erythema edema with intense erythema edema and erythema with bleeding (intense erythema with edema and vesicle) 1234

ingredient Content (% by weight) Comparative Example 2 Example 7 Example 8 Example 9 Example 10 Example 11 Example 12 Bokbunja Extract (Reference Example 1) - 1.00 - - 1.00 1.00 1.00 Blackberry Extract (Reference Example 2) - - 1.00 - 1.00 - 1.00 Fruit Strawberry Extract (Reference Example 3) - - - 1.00 - 1.00 1.00 Retinol 3000 IU 3000 IU 3000 IU 3000 IU 3000 IU 3000 IU 3000 IU Beeswax 4.00 4.00 4.00 4.00 4.00 4.00 4.00 Polysorbate 1.50 1.50 1.50 1.50 1.50 1.50 1.50 Sorbitan sesquioleate 0.70 0.70 0.70 0.70 0.70 0.70 0.70 Liquid paraffin 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Caprylic / Capric Triglycerides 5.00 5.00 5.00 5.00 5.00 5.00 5.00 glycerin 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Butylene glycol 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Propylene glycol 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Carboxy Vinyl Polymer 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Triethanolamine 0.20 0.20 0.20 0.20 0.20 0.20 0.20 antiseptic Quantity Quantity Quantity Quantity Quantity Quantity Quantity Pigment Quantity Quantity Quantity Quantity Quantity Quantity Quantity Spices Quantity Quantity Quantity Quantity Quantity Quantity Quantity Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100

Composition Stimulation Relief (%) * Example 7 26 Example 8 32 Example 9 35 Example 10 43 Example 11 28 Example 12 19 Comparative Example 2 -

From Table 11, it was confirmed that the formulation containing at least one extract of Bokbunja, Blackberry and Blackberry extract of the present invention has the effect of inhibiting erythema induced by retinol in human skin.

Test Example 8 Evaluation of Efficacy of Skin Erythema by UV Rays in a Raspberry Extract-Containing Formulation by Human Test

Healthy volunteers who had not taken nonsteroidal anti-inflammatory and other steroidal agents for a month prior to the trial were evaluated according to Kaidbey and Kurban's method for inhibiting the skin erythema induced by UVB of raspberry extract (UVB). Kaidbey & Kurban, 1976, J. Invest.Dermatol., Vol 66 pp 153-156). In other words, an opaque tape with six holes of 1.5 cm in diameter was attached to the upper forearm of 12 healthy males, and twice the amount of ultraviolet light (Minimun Erythema Dose, MED) of each subject was measured. UVB) was irradiated with SE lamp (wavelength 290 ~ 320nm, Toshiba) to induce erythema. Each emulsion formulation prepared according to the formulation of Table 12 was applied 1 hour before and immediately after UV irradiation. The erythema index after 6 hours and 24 hours after ultraviolet irradiation was evaluated, and the results are shown in Table 13 below.

ingredient Content (% by weight) Comparative Example 3 Example 13 Example 14 Example 15 Example 16 Example 17 Example 18 Bokbunja Extract (Reference Example 1) - 1.00 - - 1.00 1.00 1.00 Blackberry Extract (Reference Example 2) - - 1.00 - 1.00 - 1.00 Fruit Strawberry Extract (Reference Example 3) - - - 1.00 - 1.00 1.00 Beeswax 4.00 4.00 4.00 4.00 4.00 4.00 4.00 Polysorbate 1.50 1.50 1.50 1.50 1.50 1.50 1.50 Sorbitan sesquioleate 0.70 0.70 0.70 0.70 0.70 0.70 0.70 Liquid paraffin 5.00 5.00 5.00 5.00 5.00 5.00 5.00 Caprylic / Capric Triglycerides 5.00 5.00 5.00 5.00 5.00 5.00 5.00 glycerin 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Butylene glycol 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Propylene glycol 3.00 3.00 3.00 3.00 3.00 3.00 3.00 Carboxy Vinyl Polymer 0.10 0.10 0.10 0.10 0.10 0.10 0.10 Triethanolamine 0.20 0.20 0.20 0.20 0.20 0.20 0.20 antiseptic Quantity Quantity Quantity Quantity Quantity Quantity Quantity Pigment Quantity Quantity Quantity Quantity Quantity Quantity Quantity Spices Quantity Quantity Quantity Quantity Quantity Quantity Quantity Purified water to 100 to 100 to 100 to 100 to 100 to 100 to 100

Composition Erythema index 6 hours later 24 hours later Comparative Example 3 1.7 2.2 Example 13 1.0 1.6 Example 14 0.8 1.8 Example 15 1.2 1.8 Example 16 0.7 1.6 Example 17 0.9 1.7 Example 18 0.7 1.5

From Table 13, it can be seen that the formulation containing one or more extracts selected from Bokbunja extract, Brussels berry extract, and Brussels berry extract have the effect of inhibiting erythema induced by ultraviolet light in human skin.

From these test results, by containing at least one selected from the Bokbunja extract, Brussels berry extract, and Brussels berry extract of the present invention in the composition for external application of the skin, it is possible to suppress factors causing skin irritation and inflammation, through which various skin It can be seen that to reduce or improve the irritation and inflammation of skin cells due to the use of stress sources and cosmetics.

As described above, the composition containing raspberry extract, such as bokbunja, raspberry, or rapeberry extract, has the effect of inhibiting the production of endogenous inflammatory mediators and preventing skin irritation caused by ultraviolet rays or cosmetic use. It can be used as a cosmetic composition.

Claims (6)

A topical skin composition comprising raspberry extract in an amount of 0.00001 to 30% by weight. The composition for applying the external of the skin according to claim 1, wherein the extract is a leaf or fruit extract of at least one plant selected from Rubus coreanum, Rubus idaeus, and Rubus parvifolius. The composition for external application for skin according to claim 1, wherein the composition prevents skin irritation and inflammation by inhibiting production of IL-6 by substance P (substance P). The composition for external application for skin according to claim 1, wherein the composition inhibits the production of IL-6 and IL-8 by bradykinin, thereby preventing skin irritation and inflammation. The external preparation for skin according to claim 1, wherein the composition prevents skin irritation and inflammation by reducing the activity of cyclooxygenase-2 (COX-2) induced by ultraviolet rays to inhibit the production of PGE2. Composition The external preparation composition for skin according to claim 1, wherein when the external preparation is a cosmetic, it prevents skin irritation and inflammation caused by skin irritation components contained in the cosmetic.