KR100671847B1 - Agent for reducing somatic cell of bulk milk - Google Patents

Abstract

The present invention relates to a somatic cell reducing agent of milking cows for reducing somatic cells in milk milked from cows and the like, and more particularly, stevia extract includes any one or more selected from chitin, chitin derivatives, chitosan and chitosan derivatives. It relates to a somatic cell reducing agent of milking cows and a method for producing the same.

The somatic cell decay agent of the milking cows can dramatically reduce the number of somatic cells when orally administered to cows to obtain crude milk of good quality, and can also prevent and treat mastitis of cows in advance, as well as continuously oral administration. Even if it does not produce resistance to various mastitis causative bacteria.

Dairy cow, mastitis, somatic cells

Description

AGENT FOR REDUCING SOMATIC CELL OF BULK MILK}

The present invention relates to a somatic cell reducing agent of milking cows for reducing somatic cells in milk milked from cows and the like, and more particularly, stevia extract includes any one or more selected from chitin, chitin derivatives, chitosan and chitosan derivatives. It relates to a somatic cell reducing agent of milking cows and a method for producing the same.

The somatic cell count in the milk milked from the cow is closely related to the health status of the cow's breast. When the pathogenic microorganisms invade and infect the cow's breast, the somatic cell count increases in the crude milk due to the inflammatory state of the mammary gland, and the somatic cell count decreases when the cow's breast state is healthy.

Expensive high-quality crude oil refers to crude oil that has fewer somatic cells, fewer bacteria, and does not mix antibiotics and other foreign substances. Mastitis in cows is one of the major factors that directly lowers dairy farmer's income by decreasing the quality of milk by increasing somatic cell count.

In order to milk higher quality crude milk from cows, various methods of preventing and treating cow mastitis have been proposed, among which antibiotics and chemotherapeutic agents have been widely used.

However, these antibiotics and chemotherapeutic agents have a problem in that the resistance to various mastitis causative bacteria is used when the cow is used continuously, and the drug is not exerted.

The present invention to solve this problem can dramatically reduce the number of somatic cells in milk milked from cows to improve the quality of crude milk and effectively prevent and treat mastitis of cows, and also resistant to various mastitis causing bacteria even if used continuously to cows It is an object of the present invention to provide a somatic cell decay agent of a milking cow that does not occur and a method of manufacturing the same.

The present invention devised to achieve the above object is to concentrate the extract extracted from stevia plant tissue, fermented and aged, and then adding any one or more selected from chitin, chitin derivatives, chitosan and chitosan derivatives to the mature concentrate. It provides a method for producing a somatic cell reducing agent of milking cows comprising the step.

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It provides a somatic cell reducing agent of the milking cow prepared by the method for producing a somatic cell reducing agent of the milking cow.

Hereinafter, the somatic cell reducing agent of the milking cow of the present invention and the manufacturing method thereof will be described in detail.

The somatic cell reducing agent of the milking cow of the present invention comprises stevia extract and chitosan.

The stevia extract is dried and pulverized stevia leaves and stems, soaked in the water and then heated to extract the active ingredient, the concentrated stevia concentrate, and the stevia concentrate is fermented and aged under a suitable environment from bacteria Beneficial ingredients include the resulting mature solution. Such stevia extract has a sugar content of 200 to 300 times that of sugar but 1/90 of the calories compared to sugar, and antioxidant activity is about 5 to 7 times that of green tea.

In more detail, the stevia concentrate comprises a drying step, such as a stevia leaf, a grinding step, an extraction step and a concentration step.

The drying process is a step of sufficiently growing the stevia plant, selecting and harvesting the leaves and stem portions of the stevia plant, and the natural leaves or forced drying of the collected leaves and stem portions. Although the leaves and stems may be naturally dried, forced drying using a dryer is recommended to quickly and uniformly dry the leaves and stems. It is usually dried at 75 ± 15 ° C for about 1 day.

Then, the stevia leaves and stems dried by the drying process are subjected to a pulverization process to grind to a predetermined size so that the effective ingredients contained in the stevia leaves and stems more effectively during the subsequent extraction process.

Next, the extraction process is a step of extracting the active ingredient from the stevia leaves and stems by heating the pulverized product pulverized in the crushing step into an extraction container containing water.

Stevia leaves and stems to be put into the extraction container may be extracted only the leaves or the active ingredient using only stevia stem, depending on the intended use, may also be extracted by mixing the appropriate amount of stevia leaves and stem.

In order to more effectively extract the active ingredient from the pulverized product, other solvents may be mixed with water, and the active ingredient may be extracted from the pulverized product at a pressure higher than atmospheric pressure.

When the active ingredient is extracted from the milled product under pressure above atmospheric pressure, the boiling point of the milled water and the like is increased, thereby effectively extracting the active ingredient contained in the milled product.

In addition, the concentration step is a step of concentrating the extract extracted from the stevia plant tissue, transfer the extract extracted in the extraction step to a concentration tank, and then heat the concentration tank to obtain a concentrate from the extract.

At this time, it is preferable to concentrate by adding heat to the concentration tank while stirring the extract of the concentration tank, which is to prevent the active ingredient of stevia to stick to the inner peripheral surface of the concentration tank.

And it is preferable to concentrate an extract liquid by applying heat to a concentration tank in the state which made the atmosphere in a concentration tank into the pressure below atmospheric pressure. This means that when the pressure in the concentration tank is below atmospheric pressure, the boiling point of the extract is relatively low compared to the boiling point at atmospheric pressure, so that less energy is consumed and not only maximizes energy efficiency but also greatly shortens the time required for concentration. There is an advantage to this.

In this way, a stevia concentrate is obtained.

And the stevia aging liquid refers to a solution obtained by fermenting the stevia concentrated solution after aging for a certain time.

In the method of obtaining the aging solution from the stevia concentrate, the concentrate is fermented at a temperature of 20 to 30 ° C., and then aged for a certain period of time. And the fermentation process can be carried out by the method of fermenting the concentrate by the yeasts present in the natural state, it is preferable to shorten the fermentation period by promoting the fermentation by treating the previously cultured yeasts in the concentrate.

In the fermentation process, it is preferable to inject oxygen into the concentrate at regular intervals. Periodically injecting oxygen into the concentrate as yeast bacteria involved in fermentation are mainly aerobic microorganisms, which promotes fermentation and proliferation of yeast bacteria involved in fermentation. Providing there is an advantage that can prevent the fermentation caused by the decay bacteria during fermentation.

At this time, it is preferable to inject oxygen into the low water level of the concentrate, and more preferably, to the bottom of the concentrate. This is because oxygen supplied to the bottom of the concentrate is increased by buoyancy so that not only the yeasts present on the surface but also the yeasts growing on the bottom are energized, and the decayed bacteria can grow relatively. This is to prevent corruption by providing a clean environment.

In addition, chitosan is a substance present in crustaceans such as crabs and shrimps. Such chitosan enhances immune enhancement, antibacterial action and calcium promoting action.

The chitosan herein includes all chitin, chitin derivatives, chitosan and chitosan derivatives, and among them, it is preferable to use low molecular weight chitosan and chitosan oligosaccharides that are well dissolved in stevia concentrate and mature solution, and have excellent absorption in the body.

The chitosan may be contained in the stevia concentrate and used, or may be contained in a stevia fermentation broth or a stevia aging solution. And when using the stevia aging liquid, the chitosan may be added after the concentration step, the fermentation step or after the aging step in the stevia aging liquid preparation step.

Hereinafter, the somatic cell reducing agent of the milking cow of the present invention will be described in detail with reference to Examples. The scope of the present invention is not limited to the following Examples.

Example 1

Stevia leaves and stems were dried, pulverized and mixed, and then placed in permeable heat-resistant sacks 3kg each. Into the extraction vessel containing water, two heat-resistant sacks containing crushed powder were added, and heated to a temperature of 110 ± 10 ℃ for 2.5 hours to extract the active ingredient contained in stevia. The extracted extract was placed in a concentration bath and heated to a temperature of 110 ± 10 ° C. under negative pressure for 13 to 15 hours while stirring to obtain a concentrate. The concentrated solution was put in a fermentation vessel and then fermented at 20 to 30 ° C. for 5 days while granular activated carbon was treated. After adding 2% of chitosan oligosaccharides to the fermented fermentation broth, approximately 150 at 20 to 30 ° C. Aged daily to complete the somatic cell decay agent of the milking cow.

Example 2

Unlike Example 1, Example 2 of the present invention, after adding 10% chitosan oligosaccharide to the fermentation broth, aged at 20 to 30 ° C. for about 150 days to complete the somatic cell reducing agent of the milking cow.

Example 3

And in Example 3, unlike in Example 1, 20% chitosan oligosaccharide was added to the fermentation broth, and then aged for 20 days at 20 to 30 ° C. to complete the somatic cell reducing agent of the milking cow.

The somatic cell decay agent of the milking cow of Example 1 was thus orally administered to 6 cows of 1 cow for 40 weeks every day for 6 weeks, and the somatic cell decay agent of the milking cow of Example 2 was 40cc of cows of 6 cows of 2 cows daily. Six weeks was given orally, and the somatic reduction agent of the milking cow of Example 3 was orally administered to the six cows of three cows for 40 weeks each day for six weeks.

After 6 weeks, the somatic cell decay agent of the milking cows of Examples 1 to 3 was measured and compared with the somatic cell count of the milk milked before oral administration of the somatic cell reducing agent of the milking milk, The results are shown in Table 1 below.

[Table 1] Changes in somatic cell number following oral administration of somatic cell depressants in milking cows

Kinds Administration head Dosage Average somatic cell count Before dosing After administration Example 1 6 heads 40 cc 840 thousand / ml 375 thousand / ml Example 2 6 heads 40 cc 876 thousand / ml 343 thousand / ml Example 3 6 heads 40 cc 893 thousand / ml 327 thousand / ml

As shown in Table 1, it can be confirmed that the somatic cell counts after the administration of the somatic cell reducing agents of the milking cows of Examples 1 to 3 were significantly reduced after administration to the cows.

Crude milk with fewer somatic cells refers to crude milk derived from healthy breasts of cows not infected with mastitis, and thus the present invention has excellent prophylactic and therapeutic effects against mastitis of cows.

As described above, the somatic cell decay agent of the milking cow of the present invention rapidly reduces somatic cell counts when orally administered to cows to obtain crude milk of high quality, and also prevents and treats mastitis of cows in advance. Repeated oral administration is effective in preventing resistance to various mastitis causative organisms.

In addition, by adding chitosan to the fermentation and aging of the stevia concentrate to prepare the somatic cell reducing agent of the milking cow, there is an effect that can be mass-produced somatic cell reducing agent of the milking cow by a simple manufacturing method.

In addition, by injecting air periodically during fermentation and maturation of stevia concentrate, it is possible to significantly shorten the manufacturing time of the somatic cell reducing agent of milking cows and to prevent corruption.

Claims (3)

Concentrating the extract extracted from the stevia plant tissue, and fermentation and fermentation, and the method of producing somatic cell decay agent of the milking cow comprising the step of adding chitosan oligosaccharides to the mature concentrate. The somatic cell-reducing agent of the milking cow characterized by the manufacturing method of the somatic cell-reducing agent of the milking cow of Claim 1. delete