KR100663969B1 - The production method for distillate including Chrysanthemum, Pinanceae, Acanthopanax and Rhamnaceae and the distillate thereof - Google Patents

Abstract

The present invention relates to a method for producing a medicinal herb containing medicinal plant distillate extracted from wild chrysanthemum leaves, pine needles, thorny scabbard and bark leaf and the medicinal herb prepared by the above method.

According to the present invention, the leaves of pine chrysanthemum, pine needles, stems and leaves of thorny opiaceae, leaves of huts, hot water extraction, organic solvent extraction, residue extraction, and then the extracts are mixed, filtered and distilled to obtain medicinal plant distillate. By preparing and adding to purified water, there is an effect having resistance to E. coli and Pseudomonas aeruginosa.

In addition, the medicinal herbs of the present invention have a significant negative ions to increase the activity of the alpha (α) wave to relieve asthma and migraine headaches, serotonin (Serotonine) and free histamine (Free Histamine), a neurohormone that causes respiratory diseases such as asthma It has a suppressing effect.

Medicinal Plant Distillate, Wild Chrysanthemum, Pine Needle, Prickly Pear

Description

The production method for medicinal plants containing medicinal plant distillates, including wild chrysanthemum leaves, pine needles, thorns, and bark leaves and the medicinal herb prepared by the above method {The production method for distillate including Chrysanthemum, Pinanceae, Acanthopanax and Rhamnaceae and the distillate}

1 is a manufacturing process of the pharmacological plant distillate according to the present invention.

Figure 2 is a manufacturing process of the medicinal herbs containing pharmacological plant distillate according to the present invention.

3 is a test result of resistance to Pseudomonas aeruginosa of the pharmacological distillate of the present invention.

Figure 4 is a test result of the resistance to E. coli of the pharmacological distillate of the present invention.

The present invention relates to a method for producing a medicinal herb containing medicinal plant distillate, including wild chrysanthemum, pine needles, thorny stems, bark leaves, and medicinal herbs prepared by the above method.

In 2000, the topic of the beverage market was 'natural'. The so-called 'natural drinks' made of natural materials, such as fruit juices such as mango and citrus fruits and soy milk, have been loved by consumers thanks to the continuous trend of healthy drinks.

Negative growth in the 100% normal orange juice market means that the market for 100% general orange juice in addition to functional and refrigerated distribution juice is gradually being replaced by citrus juice and other juices.

Like oranges, citrus juices are loved a lot because, unlike oranges, they are familiar to us, and they are less sour and sweeter than oranges. In addition, it is rich in vitamin C, which is effective for skin beauty and cold prevention, and is rich in beta-carotene, the mother of vitamin A, to help prevent cancer.

As the trend shows, beverages are not only quenching thirst but special functionalities that affect human physiology are emphasized, and large conglomerates and small and medium-sized companies are competing in the market competition. Fruit drinks, functional drinks, traditional drinks, Market competition is intensifying due to the rapid increase of new companies' participation due to the creation of new markets such as bottled water, but bottled water is easily exposed to Escherichia coli and other pathogens, causing many problems.

An object of the present invention for solving the above-mentioned problems is to provide a method for producing a medicinal herb containing medicinal plant distillate, including wild chrysanthemum leaves, pine needles, thorny scabbard, bark leaf.

The present invention for solving the above technical problem is a method and preparation of medicinal herbs containing medicinal plant distillate, including wild chrysanthemum leaves ( Chrysanthemum ), pine needles ( Pinaceae ), prickly pear ( Araliaceae ), leaf of Hovenia Dulcis It is about.

The wild chrysanthemum leaves of Chrysanthemum are choline, stachydrin, adenine, vitamin A, vitamin B ₁ , apigenin, apigetrin, 16-β-hydroxy-3-fucedota esterol (16-β-Hydroxy-3-pseudotaraxasterol), Chlorogenic acid, Taxasterol, contains about 1.5% essential oils, and these components are detoxifying, antiviral, and tubular Artery and coronary vein expansion, blood flow increase, blood pressure lowering, antiviral and antibacterial, antipathogenic action.

In addition, pine needles of Pinaceae are ascorbic acid (Vitamin C), carotene (Carotene), vitamin K, vitamin B, flavonoids (Anthocyan), tannin (Tannin), carbohydrate uniper It contains 0.13% to 1.3% of terevin oil (essential oil), which is composed mainly of acid and P-nonaco acid.

In addition, the main component of the thorny stem of the Araliaceae family is eleutherosides, and seven major eleutherosides were identified, among which eleutherosides B and E. Is being studied intensively. Eleutheside B acts as sedation, insomnia, forgetfulness, gonadotropin, lowering blood pressure, autonomic nerve adjustment, improving glucose metabolism, preventing complications of diabetes, and improving liver function. Anti-stress, gonadotropin, learning improvement, endurance, stamina, concentration, antirheumatic, etc. In addition, the barb contains complex polysaccharides, so these components play an important role in improving immune function.

In addition, Hovenia Dulcis , which belongs to the sea buckthorn family, contains fruit, roots, branches, and leaves with glucose, malic acid and calcium, as well as beneficial components in the human body, such as furangurinine, hobenin, hovanoside, and habenic acid. Detoxification, fatigue recovery, diabetes treatment effect, "amperopsin and hovenitins" is an activating substance to protect the liver and 90% cancer cell eradication effect on various cancers (liver cancer, breast cancer, stomach cancer) .

Hereinafter, the configuration of the present invention will be described in detail.

Wild chrysanthemum leaves, pine needles, thorn stems and leaves, leaves of the barn tree first dried in the shade, slightly boiled in brine, dried in the shade and dried again in the sun to prepare a medicinal plant distillate as shown in FIG.

First step: undiluted solution manufacturing process

1 kg of dried wild chrysanthemum leaves, 1 kg of pine needles, 1 kg of stems and leaves of thorns, and 4 kg of leaves of 1 kg of walnut trees were pulverized with a grinder, and 20 liter of water was added to the extract in a heated extract and wild chrysanthemum leaves and pine needles. , Prickly vine leaves, bark leaves, thorny stem stems and extract the primary decompression 1 ~ 2 hours at 40 ~ 50 ℃, and extracted under reduced pressure 1 ~ 2 hours at 70 ~ 90 ℃ again to prepare a stock solution.

Second Process: Concentrate Manufacturing Process

30 l of ethyl alcohol was added to 1 kg of dried wild chrysanthemum leaves, 1 kg of pine needles, 1 kg of thorny stems and leaves, and 1 kg of leaves of hawthorn trees, and heated at 70 ° C. for 3 hours, and then repeated twice to extract pharmacological extracts. The pharmacological extract is concentrated under reduced pressure with a 70 cmHg pressure reduced pressure extractor, and sterilized at 80 to 100 ° C. for 20 to 40 seconds to prepare a concentrate.

Third step: extract manufacturing process

The residues of the first and second processes are again extracted with microwave to prepare an extract.

Fourth process; Filtration and Distillation Process

The stock solution of the first step, the concentrate of the second step, the extract of the third step, and three times the water of the undiluted solution were mixed firstly with Whatman No. 1 paper, followed by a 0.2 μm membrane. Second filter with filter). Steaming the filtrate is collected by distillation to collect the water vapor in a distillation tube, cooled by a cooler of 0 ~ 5 ℃ and condensation obtained by distilling the pharmacological condensate obtained by distillation of the pharmacological plant.

An example of the preparation ratio of the pharmacological plant distillate is shown in Table 1 below, and can be adjusted in the blending ratio shown in Table 1.

Example of the preparation ratio of pharmacological plant distillate (unit: weight%)  Dry wild chrysanthemum leaves  Dried pine needles Dried spiny stems and leaves  Dry barn leaves  sub Total 25.0 25.0 25.0 25.0 100

The manufacturing process of the medicinal herb containing the pharmacological plant distillate prepared as described above in detail with reference to FIG.

First step: mixing process

10.0 ~ 20.0% by weight of pharmacological plant distillate, 80.0 ~ 90.0% by weight of purified water are quantified and added to purified water and mixed.

Second Step: Stirring the Mixture

The mixed solution mixed by the first step is stirred with a rotary stirrer.

Third Step: Filtration Step of Stirring Solution

The stirring liquid stirred by the said 2nd process is filtered by the filtration filter for purification.

4th step: Packing process

The beverage containing the pharmacological plant distillate, which has been manufactured by the third process, is packed by washing and sterilizing containers such as bottles and cans.

Table 2 shows an example of the preparation ratio of the beverage containing the pharmacological distillate by the manufacturing process.

Formulation ratio of medicinal herb containing pharmacological distillate (unit: wt%) Example 1 Example 2 Example 3 Purified water 80.0 85.0 90.0 Pharmacological Plant Distillate 20.0 15.0 10.0

Based on the above Table 2, the configuration of the present invention will be described in more detail based on the embodiment. However, the scope of the present invention is not limited to the claims of the present invention by the embodiment.

Example 1. Preparation of medicinal herb containing pharmacological distillate (1)

1 kg of dried wild chrysanthemum leaves, 1 kg of pine needles, 1 kg of stems and leaves of thorns, and 4 kg of leaves of 1 kg of walnut trees were pulverized with a grinder, and 20 liter of water was added to the extract in a heated extract and wild chrysanthemum leaves and pine needles. , Prunus oak leaves, bark leaves, prickly ivy stems were extracted under reduced pressure at 45 ° C. for 2 hours and again under reduced pressure at 80 ° C. for 1 hour to prepare a stock solution. Separately, 1 kg of dried chrysanthemum leaves, 1 kg of pine needles, 1 kg of stems and leaves of thorns, 1 kg of leaves of huts were added 30 liters of ethyl alcohol and heated at 70 ° C. for 3 hours to extract the pharmacological extracts. The extract was concentrated under reduced pressure with a 70 cmHg pressure reducer and sterilized at 80 ° C. for 20 seconds to prepare a concentrate. The stock solution and the concentrate were prepared, and the remaining residue was extracted again with microwave to prepare an extract. The stock solution, the concentrate, the extract, and three times the water of the stock solution were mixed and filtered first with Whatman No. 1 paper, and secondly with a 0.2 μm membrane filter. The filtrate was heated and evaporated to collect vapor in a distillation tube, cooled by a cooler at 0-5 ° C., and then condensed to obtain the pharmacological condensate obtained by distillation. 20.0% by weight of the pharmacological plant distillate and 80.0% by weight of purified water were mixed, sterilized, stirred and filtered to prepare a herbal solution containing pharmacological distillate.

Example 2. Preparation of medicinal herb containing pharmacological distillate (2)

1 kg of dried wild chrysanthemum leaves, 1 kg of pine needles, 1 kg of stems and leaves of thorns, 1 kg of leaves of huts, 4 kg each were pulverized with a grinder, and 20 liters of water was added to the extract in a heated extract and wild chrysanthemum leaves and pine needles. , Prunus oak leaves, shed leaves and thorn stems were extracted at 45 ° C for 1 hour under reduced pressure, and again extracted under reduced pressure at 80 ° C for 1 hour to prepare a stock solution. Separately, 1 kg of dried chrysanthemum leaves, 1 kg of pine needles, 1 kg of stems and leaves of thorny oak, and 1 kg of leaves of huts were added 30 l of ethyl alcohol, and heated at 70 ° C. for 3 hours to extract the pharmacological extracts. The extract was concentrated under reduced pressure with a 70 cmHg pressure reducer and sterilized at 90 ° C. for 30 seconds to prepare a concentrate. The stock solution and the concentrate were prepared, and the remaining residue was extracted again with microwave to prepare an extract. The stock solution, the concentrate, the extract, and three times the water of the stock solution were mixed and filtered first with Whatman No. 1, and secondly with a 0.2 μm membrane filter. The filtrate was heated and evaporated with steam to collect in a distillation tube, cooled with a condenser of 2.5 ℃ and condensation obtained by distilling the pharmacological condensate obtained by distillation to prepare a pharmacological plant distillate. 15.0% by weight of the pharmacological plant distillate and 85.0% by weight of purified water were mixed, sterilized, stirred and filtered to prepare a herbal solution containing pharmacological distillate.

Example 3. Preparation of medicinal herb containing pharmacological distillate (3)

1 kg of dried wild chrysanthemum leaves, 1 kg of pine needles, 1 kg of stems and leaves of thorns, and 4 kg of leaves of 1 kg of walnut trees were pulverized with a grinder, and 20 liter of water was added to the extract in a heated extract and wild chrysanthemum leaves and pine needles. , Prunus oak leaves, shed leaves and thorn stems were extracted at 45 ° C for 1 hour under reduced pressure, and again extracted under reduced pressure at 80 ° C for 1 hour to prepare a stock solution. Separately, 1 kg of dried chrysanthemum leaves, 1 kg of pine needles, 1 kg of stems and leaves of thorny oak, and 1 kg of leaves of huts were added 30 liters of ethyl alcohol and heated at 70 ° C. for 3 hours to extract the pharmacological extracts. The extract was concentrated under reduced pressure with a 70 cmHg pressure reducer and sterilized at 100 ° C. for 40 seconds to prepare a concentrate. The stock solution and the concentrate were prepared, and the remaining residue was extracted again with microwave to prepare an extract. The stock solution, the concentrate, the extract, and three times the water of the stock solution were mixed and filtered first with Whatman No. 1, and secondly with a 0.2 μm membrane filter. The filtrate was heated and evaporated to collect steam in a distillation tube, cooled by condensation at 5 ° C., and condensed to obtain the pharmacological condensate obtained by distillation. 10.0% by weight of the pharmacological plant distillate and 90.0% by weight of purified water were mixed, sterilized, stirred and filtered to prepare a herbal solution containing pharmacological distillate.

In addition, the present invention is in addition to the wild chrysanthemum, bark, thorns, pine needles and pine needles, licorice, gojija, tofu, mountain liquids, bokbunja strawberry, squid, schisandra, violet, branch, scented oil, thistle, cheonma, nectar, dandelion, larch, mistletoe, sewage , Astragalus, Cinnamon, Golden, Gemini, Bellflower, Deodeok, Mugwort, Mugwort, Health, Angelica, Chinese quince, Cypress, Chariot, Fennel, Triticales, Emulsion, Sukjihwang, Dansam, Poisonous, Safflower, Safflower seed, Small head A medicinal herb containing pharmacological distillate can be prepared by the method prepared in Examples 1, 2 or 3 by adding a passerby.

In order to determine whether the pharmacological plant distillate prepared in Example 1 has antimicrobial activity, the antibacterial activity against E. coli and Pseudomonas aeruginosa and anion generation rate were tested.

Test Example 1. Examination of antimicrobial activity of pharmacological plant distillate

Escherichia coli (Escherichia coil ATCC No.25922) and Pseudomonas aeruginosa ATCC No. 15442 were used as test bacteria, and broth, agar medium, and Tryptone Glucose Extract agar medium were used as a medium.

In order to incubate the specimens, a 4 mm loop was used to transplant 10 ml of broth (5.1) from the small intestine and incubated at 37 ° C. for 24 hours. Strains in storage were stored in a slope incubator of agar medium and stored strains were stored at 5 ° C. and passaged once a month.

Juicy juice cultured for 24 hours so that the number of bacteria regenerated from the test group (treated with pharmacological plant distillate) or the control group (without pharmacological plant distillate) at the inoculation time “0” is 4-6 × 10 ^5. The culture microbial solution was diluted appropriately and 5 ml of this solution was used as the inoculum. At this time, the dilution of the culture bacteria was used saline solution.

5 mL pharmacological plant distillate was added to the test zone. When using E. coli, bacteria cultured for 24 hours were shaken and left standing for 15 minutes before preparing the inoculum. Using a pipette, 5 ml of inoculum (for each container) was carefully dropped to distribute evenly on the test and control.

The pharmacological plant distillate was expressed as the number of bacteria or the reduction rate, and was expressed by two significant figures.

The results according to Test Example 1 are shown in Table 3 below and FIGS. 3 and 4.

Results of Antimicrobial Activity of Escherichia Coli and Pseudomonas Aeruginosa in Pharmacological Plant Distillates Test Items Sample classification Initial concentration Concentration after 24 hours Bacteriostatic reduction (%) Antibacterial test by E. coli Control 1.8 × 10 ⁶ 5.2 × 10 ⁶ - Test <1.0 × 10 ⁴ 99.9 Antibacterial test by Pseudomonas aeruginosa Control 3.2 × 10 ² 9.1 × 10 ⁷ - Test 1.4 × 10 ⁶ 95.6

As shown in Figures 3 and 4, the experimental group treated with the pharmacological plant distillate of the present invention showed no growth of E. coli and Pseudomonas aeruginosa, but the control group significantly increased colonies of E. coli and Pseudomonas aeruginosa.

Therefore, wild chrysanthemum, pine, thorny oak bark, and barn tree, which are raw materials of the present invention, proved to have antibacterial properties.

Test Example 2 Measurement of Emissivity of Distillate of Pharmacological Plants

In this test example, the emissivity was measured at 40 ° C with the black body furnace as the contrast side, and the emissivity was measured using an infrared spectrometer with a wavelength range of 520 µm with haebsil waterproof mortar at the same temperature. Pharmacological plant distillate was taken as a sample to prepare a black body using a Fourier transform infrared spectrometer (FT-IR-Spectrometer model; FT / IR-4000 / 6000Series, manufacturer; Sungjin I & T Co., Ltd.) The results are shown in Table 4 below.

Far-infrared Incidence of Pharmacological Plant Distillates Emissivity (520㎛) Radiation energy (W / ㎡ · ㎛, 37 ℃) 0.902 3.48 × 10 ²

Far-infrared emissivity should be more than 0.89 ~ 0.90 to emit far infrared rays, the present invention showed an emissivity of 0.902. Black body (BLACK BODY) is based on the imaginary material 100% of far infrared rays, a virtual object that completely absorbs all radiation (light), the far-infrared emissivity of the distillate of pharmacological plants of the present invention is measured as 90.2% and converted to 0.902 Indicated.

Test Example 3 Measurement of Anion Generation in Pharmacological Plant Distillates

Anion released from 100 ml of pharmacological plant distillate of the present invention was tested at room temperature 25 ℃, humidity 43%, anion water 108 / 보 condition using a particle charge measuring instrument (model; PCD, Manufacture; Sambo Science) Was measured and expressed as the number of IONs per unit volume.

Anion Generation Rate of Pharmacological Plant Distillates Anion (ION / ㏄) Pharmacological plant distillate (100ml) 188

As shown in Table 5, the result of Test Example 2 generated 188 anions per 100 ml of pharmacological plant distillate. Negative ions are negatively charged ions, and Dr. Kruger of the University of California, Berkeley, and Dr. Sulman of the University of Hebrew, Jerusalem, reported that more than 1,000 anions per air. Enriched by the brain increases the activity of alpha (α) to relieve the worry and tension that causes asthma and migraines. It also has the effect of inhibiting serotonin and free histamine, which are neurohormones that cause respiratory diseases such as asthma.

In light of the above research results, the pharmacological distillate of the present invention could also be inferred to have an effect of inhibiting serotonin and free histamine.

Test Example 4 Sensory Evaluation of Beverages Containing Pharmacological Plant Distillates

Drinks containing the pharmacological plant distillates prepared in Examples 1, 2, and 3 were drinked and the degree of palatability was tested. The sensory test was carried out by a five-point evaluation method for a total of 80 people, the results of the sensory test are shown in Table 6 below.

Sensory Test Results of Beverages Containing Pharmacological Plant Distillates Example 1 Example 2 Example 3 color 4.5 4.6 4.7 flavor 4.8 4.7 4.8 incense 4.2 4.3 4.8 Overall preference 4.6 4.7 4.7 1: very dislike, 2: dislike, 3: normal, 4: good, 5: very good

The results of the sensory test according to Table 6 were generally good in terms of color, taste, aroma, and overall acceptability. Particularly, a good result in terms of color did not affect the color of the beverage even if pharmacological distillate was added. Because it did not.

As a result of the sensory test as described above, the present invention can be ingested alone, and even when added to a drink, the preference is high.

The present invention is not limited to the technical spirit of the contents described in the specific embodiments, test examples, diagrams or drawings described above, and the general knowledge in the technical field to which the present invention pertains without departing from the gist of the present invention as claimed in the claims. Of course, any person having a variety of modifications can be made, and such changes are within the scope of the claims.

As described above, the present invention relates to a method of preparing a medicinal herb containing medicinal plant distillates, including wild chrysanthemum leaves, pine needles, thorn leaves, and bark leaves, and the medicinal herb prepared by the above method. It is effective in resisting E. coli and Pseudomonas aeruginosa.

In addition, the present invention has the effect of eliminating the stress by radiation far infrared rays to improve the gastrointestinal state.

In addition, the present invention is an anion generation test results in a significant anion generation to increase the activity of alpha (α) waves to relieve asthma and migraine headaches, serotonin (Serotonine) and free histamine (neurohormones causing respiratory diseases such as asthma) Free Histamine) is effective.

Claims (2)

1 1 kg dried leaves of wild chrysanthemum, 1 kg of pine needles, 1 kg of stems and leaves of thorns, 1 kg of leaves of huts, 4 kg each were pulverized with a grinder, and 20 liters of water was added together with the pulverized powder in a wild extract. Preparing pine needles, thorny oak leaves, barley leaf, and thorny oak stems under reduced pressure for 1 to 2 hours at 40 to 50 ° C., and extracting for 2 hours at 70 to 90 ° C. for 2 hours. 30 1 liter of dried wild chrysanthemum leaves, 1 kg of pine needles, 1 kg of stems and leaves of thorny oak, and 1 kg of leaves of hawthorn, add 30 liters of ethyl alcohol, heat at 70 ° C. for 3 hours, and repeat twice. Extracting and concentrating the pharmacological extract with a reduced pressure extractor at a pressure of 70 cmHg and high temperature sterilization at 80 to 100 ° C. for 20 to 40 seconds to produce a concentrate; (B) extracting the residues of the first and second steps into microwaves to produce an extract; 원 the stock solution of the first step, the concentrated solution of the second step, the extract of the third step, and three times the water of the stock solution are mixed, filtered, heated at 70-95 ° C., cooled and condensed to obtain steam produced by heating. Distilling the cooling condensate to produce a pharmacological plant distillate; And 약 Pharmacological plant distillate containing wild chrysanthemum, pine needles, thorny stems, bark leaves, characterized in that the pharmacological plant distillate according to the fourth step is prepared by mixing, sterilizing, stirring, filtration and packaging with purified water Method of making a medicinal herb. Herbal medicine containing pharmacological plant distillate prepared by the method of claim 1.

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