KR100651703B1 - Tablet milk composition having immuno-modulating activity and preparation method thereof - Google Patents

Abstract

The present invention relates to a tablet-type functional milk composition and a method for preparing the same, which contain the extract of prickly pear extract, deodeok extract, mushroom mushroom extract and materi mushroom extract.

Description

Milk composition in the form of tablets to improve immune activity and a method for producing the same {TABLET MILK COMPOSITION HAVING IMMUNO-MODULATING ACTIVITY AND PREPARATION METHOD THEREOF}

The present invention relates to a functional milk composition in the form of a tablet containing a natural material which has excellent sensory properties, good palatability, and improves immune activity, and a method for producing the same.

As living standards improve, interest in health and longevity is increasing. However, due to fatigue, stress, poor eating habits, etc., the immunity is lowered, and adult diseases such as cancer are emerging as a new social problem.

According to the Western diet, various western foods are gradually increasing in Korea. Among them, the consumption of dairy products such as milk, cheese, and yogurt is gradually increasing due to the importance of children's health and development.

Domestic dairy-related industries are also expanding in line with the increasing consumption of milk products, but imports of cheap dairy products from foreign countries have also increased due to trade liberalization, which has resulted in the loss of international competitiveness of domestic dairy products and increased milk powder inventory. Under these conditions, there is a need to protect dairy farmers and milk processors by developing functional dairy products based on milk powder and increasing domestic crude oil consumption.

Until now, milk products are mainly liquid products such as milk, processed milk, or solid products such as cheese, but it is inconvenient to easily eat as cold storage and refrigeration distribution are required. Recently, there is a growing preference for convenience foods that consumers can easily and conveniently eat, and also, such as substitutes for long-term travel or climbing, armaments, nutritional supplements or snacks for those who skip meals or examinees in the morning, etc. There is a need for classes of class.

In addition, according to the recent trend of consumers who prefer natural foods having functional and palatability, there is a demand for products targeting various consumer groups having enhanced functionality and palatability. Therefore, the need to provide milk in tablet form suitable for children as well as adults is greatly increased. However, milk in tablet form using natural materials that enhance the immune activity of cells has not been studied or introduced yet.

Technical challenge

It is an object of the present invention to provide a natural material-containing tablet-type functional milk composition and a method for producing the same, having excellent sensory properties, good palatability, an ability to improve immune activity, and excellent shelf life and storage at room temperature.

Technical solution

An object of the present invention as described above provides a functional milk composition in the form of tablets, and the method of manufacturing a method of adding prickly ginseng extract, deodeok extract, mushroom mushroom extract and matari mushroom extract showing the ability to improve the immune activity of the cells in skim milk powder Is achieved.

Therefore, the purified milk composition according to the present invention is characterized by containing prickly pear extract, deodeok extract, mushroom mushroom extract, and matari mushroom extract as components for improving the immune activity of the cells.

The tablet milk composition according to the present invention may include mushroom mushroom powder instead of mushroom mushroom extract powder, and mushroom mushroom powder instead of mushroom mushroom extract powder.

Tablet milk composition according to the present invention, in addition to the above components to improve the immune activity, as other natural materials colostrum powder, ganoderma lucidum extract powder, situation mushroom extract powder, ginseng powder, lactoferrin, glutamine peptide, lactose, crystalline oligosaccharide and these It may further comprise one or more components selected from the group consisting of a combination of.

Colostrum powder contains about 20% of immunoglobulin, an immune component, which protects the body from harmful bacteria and viruses.

Ganoderma lucidum mushroom is known to be effective in preventing adult diseases and recovering from fatigue by strengthening the immune system of the human body.

Polysaccharides such as meshima of the situation mushrooms are used as anticancer and immunotherapy. The hydrothermal extracts of the situation mushrooms have been reported to have anti-cancer effects in the digestive system, and have been reported to have increased immune function when combined with chemotherapy after surgery for patients with liver cancer.

Ginseng is known to have a lot of efficacy, called a panacea, among which the tonic, anti-stress and anti-fatigue effect, hangover effect, anemia recovery, hematopoietic effect and radiation disorder defense effect, hypertension Preventive and therapeutic effects, anti-cancer effects and immune function effects, diabetes treatment effects, jeongjeong (强 精) action, anti-aging and the like.

Lactoferrin is a powerful antiviral and antimicrobial substance found only in cow's colostrum and human colostrum in nature. It is an iron-bound protein that plays an important role in combining and releasing iron. Specifically, by blocking the iron supply necessary for bacterial growth by binding with the absorbed iron to suppress bacterial reproduction, the combined iron serves as a catalyst in supplying oxygen to all cells in the body. It is known to have a prophylactic effect of disease infection, malignant tumor, AIDS, cancer.

Glutamine peptides are wheat protein hydrolysates, which are known to exert an immune effect by enhancing the epithelial cells in the intestinal tract, the body's initial defenses, which serve to protect against invasion of pathogens.

Lactose is a disaccharide consisting of glucose and galactose, which has a sweetness of 1/5 the level of sugar and acts as a binder in the manufacture of tablets.

Oligosaccharide is used by intestinal bacteria, including bifidus bacteria that reach the large intestine and inhabit the large intestine without being degraded by the intestinal digestive enzymes when ingested, and has the function of inhibiting the proliferation of harmful or pathogenic bacteria.

As can be seen in Table 1, prickly pear extract powder was found to enhance immune activity. In the present invention, as long as it is a form that can be allowed to be added to food, the extract may be any type of extract extracted using hot water or using an organic solvent.

Preferably, prickly pear extract is obtained by the following method. The stems of prickly thorns are cut, 5 to 10 times of water is added, and extracted at 90 to 100 ° C., preferably for 2 hours or more, more preferably for 2 to 3 hours. The obtained extract was filtered using a filter paper and mixed well at 60 ° C in a weight ratio of 60%: 40% of solids extract: dextrin in solids, followed by inlet temperature of 200-230 ° C, Spray dry at an outlet let temperature of 110 to 130 ° C. The amount of P. falciparum extract powder is preferably added in an amount of 3 to 7% by weight based on the total weight of the tablet milk composition, but is not limited thereto.

Deodeok is good for people who are tired of helping the liver easily, and it is known to strengthen the stomach and promote digestion. Since ancient times, Deodeok is not only a healthy food, but also famous for tonic food, and has been known as a food that strengthens lungs, spleen and kidneys. In the present invention, by adding the deodeok extract powder having such a function to the refined milk, it is possible to enhance the immune activity and also provide a function as a fatigue recovery agent. Deodeok extract powder may be added in 1 to 3% by weight, but is not necessarily limited thereto.

However, in the case where the added amount of P. falciparum extract and Deodeok extract powder is excessively large, the function of immune activity and the like can be greatly improved, but the taste and taste of tablet milk are deteriorated due to the strong taste and aroma of these ingredients. There may be. On the contrary, when the addition amount of these components is too small, a desired function may not be obtained. Therefore, the content ranges of the prickly tortoise extract powder and deodeok extract powder defined above correspond to a preferred range that can simultaneously satisfy both functionality and palatability.

Mushrooms are known to have excellent anti-cancer and antimicrobial effects. Especially, they contain an ingredient called lentinasine, which also acts as an antithrombotic effect. Matari mushrooms are known to have excellent dietary effect as they ship only the first harvested mushrooms by adding nutrients to cottonwood sawdust after selecting breeding varieties with excellent taste among 20 kinds of oyster mushrooms.

Tablet milk with improved immune activity according to the present invention is a mushroom extract powder (or mushroom mushroom powder) and mushroom mushroom extract powder (or mushroom mushroom powder) 1.0 to 3.0 weight each of the total weight of the tablet milk composition It contains in the amount of%.

Ingredients for enhancing immune activity, which are added to purified milk with improved immune activity according to the present invention, were selected as follows.

1. Selection of Immune Active Materials

Table 1 below shows the reactive nitrogen species secretion ability by macrophage (macrophage) cell line by material. As can be seen in Table 1, among the 16 kinds of natural materials, prickly pear hot water extract showed 91.93 μM, the highest nitrogen species secretion ability.

Table 1

Table 2 below shows the interleukin-1α secretion ability by macrophage cell line by material. As can be seen in Table 2, among the 16 natural materials, mushroom mushroom hot water extract showed the highest interleukin-1α secretion capacity as 203.13 pg / ml.

TABLE 2

Table 3 below shows the results of testing the immunogenic ability of the extract according to the concentration of the selected natural material extract. As can be seen in Table 3, the highest immunity was obtained when 1000 μg / ml of hydrothermal extract was added.

TABLE 3

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2. Pretreatment of Immune Active Material

Finely cut the stems of prickly pears, add water at a weight ratio of 1: 5, filter the liquid obtained by heating and extracting at 100 ° C. for 2 hours through a filter paper, and filter the prickly pear extracts: dextrin in a blending tank by 60% of solids. At a weight ratio of 40%, the mixture was mixed well at 60 ° C, and then dried with a spray dryer at an inlet temperature of 200 to 230 ° C and an outlet temperature of 110 to 130 ° C.

Deodeok, ganoderma lucidum mushroom, situation mushrooms, mushroom mushrooms and materi mushrooms were finely chopped, and then extracted in the same manner as in the case of prickly pear and mixed with dextrin to prepare an extract powder.

In addition, when mushrooms and mushrooms are added in powder form, they are finely chopped, and then dried in a drier at 50 to 60 ° C. for 24 hours and powdered in a grinder. Matari mushrooms were further ground and pulverized with a ball mill for 12 hours.

3. Formulation of materials

Tablet milk composition according to the present invention, with respect to the total weight of the milk composition, 50 to 70% by weight skim milk powder, 3.0 to 7.0% by weight of prickly pear extract powder, 1.0 to 3.0% by weight deodeok extract powder, mushroom mushroom extract powder 1.0 to 3.0% by weight (or mushroom mushroom powder) and 1.0 to 3.0% by weight of mushroom mushroom extract powder (or mushroom mushroom powder).

As an additional component, when it contains one or more components selected from the group consisting of colostrum powder, ganoderma lucidum extract powder, ground mushroom extract powder, ginseng powder, lactoferrin, glutamine peptides, lactose, crystalline oligosaccharides and combinations thereof, The content is 0 to 10% by weight colostrum powder, 0.5 to 2.0% by weight ganoderma lucidum extract powder, 0.5 to 2.0% by weight situation mushroom extract powder, 3.0 to 7.0% by weight ginseng powder, 0.2 to 1.0% by weight lactoferrin, 5.0 to 15.0 glutamine peptide Wt%, lactose 1.0-5.0 wt%, crystal oligosaccharides 1.0-3.0 wt%.

4. Mixed

In preparing a tablet milk composition according to the present invention, colostrum powder, prickly pear extract powder, deodeok extract powder, mushroom mushroom extract powder (or mushroom mushroom powder), mata mushroom extract powder (or mata mushroom powder) , Ganoderma lucidum extract powder, situation mushroom extract powder, ginseng powder, lactoferrin, glutamine peptides, lactose and crystalline oligosaccharides are first mixed, and then skim milk powder is added and mixed.

5. Ripening

The raw materials mixed in the above manner are aged at 5 to 25 ° C. for 2 to 15 hours to achieve equilibrium with the water.

6. Tablet Manufacturing

The mixed raw materials are prepared in the form of tablets of 0.15 to 2 g using a tablet maker.

7. Coating

Prepared by the method described above by spraying a solution of hydroxy propyl methyl cellulose (HPMC) at a concentration of 3% in a solvent in which purified water and fermented alcohol are mixed at a volume ratio of 1.5: 8.5. Coated tablets. Such coatings are intended to improve shelf life by preventing the components contained in the tablets from direct contact with air. Therefore, the functional milk composition of the tablet form according to the present invention can be stored at room temperature.

8. Packing

Packages of low moisture permeability, such as containers made of PVDC, are packaged in certain quantities.

Favorable effect

According to the present invention, there is provided a natural material-containing tablet functional milk composition and a method for producing the same, having excellent organoleptic properties, palatability, ability to improve immune activity, and excellent shelf life and storage at room temperature.

As can be seen in Tables 4 and 5, the tablet milk composition according to the present invention was excellent in sensory properties and palatability, it was confirmed to improve the immune activity of the cells.

In addition, since the surface of the tablet-type functional milk composition of the present invention is coated with hydroxypropylmethyl cellulose, it is excellent in storage and can be stored at room temperature.

1 is a process chart for preparing a milk composition in the form of a tablet having an ability to enhance immune activity according to the present invention.

FIG. 2 shows a procedure for measuring interleukin-1α secretion by macrophage cell lines by enzyme immunoassay (ELISA).

Embodiment for Invention

Hereinafter, the present invention will be described in more detail through Examples and Test Examples. However, these examples are merely examples of the present invention, and the scope of the present invention is not limited thereto.

Example 1

Based on the total weight of the finally obtained tablet milk composition, 5.0% by weight of colostrum powder, 3.0% by weight of prickly pear extract powder, 1.5% by weight of Deodeok extract powder, 1.5% by weight mushroom extract powder, 1.5% by weight mushroom extract powder %, Ganoderma lucidum mushroom extract powder 1.0% by weight, green mushroom extract powder 1.0% by weight, ginseng powder 5.0% by weight, lactoferrin 0.5% by weight, glutamine peptide 10.0% by weight, lactose 3.0% by weight, crystal oligosaccharides 2.0% by weight at 15 ° C. Mix with a Hobart blender for minutes. 65% skim milk powder was added to this mixture, and then all the raw materials were mixed uniformly. This mixture was then aged for 3 hours at 10 ° C. and prepared in tablet form using a tablet machine. The resulting tablet was coated with a 3% solution of hydroxypropylmethylcellulose (HPMC) to prepare a tablet milk product.

Example 2

63% by weight skim milk powder, 5.0% by weight colostrum powder, 5.0% by weight prickly pear extract powder, 1.5% by weight deodeok extract powder, 1.5% by weight mushroom extract powder, 1.5% by weight mushroom extract powder, 1.0% by weight ganoderma lucidum extract powder %, Situation mushroom extract powder 1.0% by weight, ginseng powder 5.0% by weight, lactoferrin 0.5% by weight, glutamine peptide 10.0% by weight, lactose 3.0% by weight and crystal oligosaccharide 2.0% by weight, except that the same method as in Example 1 A tablet milk product was prepared.

Example 3

61% by weight skim milk powder, 5.0% by weight colostrum powder, 7.0% by weight prickly pear extract powder, 1.5% by weight deodeok extract powder, 1.5% by weight mushroom extract powder, 1.5% by weight mushroom extract powder, 1.0% by weight ganoderma lucidum extract powder %, Situation mushroom extract powder 1.0% by weight, ginseng powder 5.0% by weight, lactoferrin 0.5% by weight, glutamine peptide 10.0% by weight, lactose 3.0% by weight and crystallization sugar 2.0% by weight, except that In the same manner a tablet milk product was prepared.

Example 4

63% by weight skim milk powder, 5.0% by weight colostrum powder, 5.0% by weight prickly pear extract powder, 1.5% by weight deodeok extract powder, 1.5% by weight mushroom mushroom powder, 1.5% by weight mushroom mushroom powder, 1.0% by weight ganoderma lucidum mushroom extract powder, In the same manner as in Example 1, except that 1.0% by weight of the mushroom extract powder, 5.0% by weight of ginseng powder, 0.5% by weight of lactoferrin, 10.0% by weight of glutamine peptide, 3.0% by weight of lactose and 2.0% by weight of crystal oligosaccharides were used. A tablet milk product was prepared.

Test Example 1: Sensory Test

Thirteen people who worked for more than two years in the field related to milk were selected as sensory test agents, and sensory tests such as color, taste, texture and overall acceptability of the refined milk prepared in Examples 1 to 4 were evaluated using a 9-point grading method. The results are shown in Table 4 below.

Table 4

Test Example 2: Immune Activity Test

In vitro immune activity [reactive nitric species secretion, interleukin-1α (IL-1α) secretion and tumor necrosis of the purified milk prepared in Examples 1-4 according to the procedure described below factor, TNF) secretion capacity].

(1) cell culture

DMEM [Dulbecco modified Eagle's medium, Gibco with or without phenol red] was used as a medium for cell culture. Culture flasks, pipettes, microplates (96-well), etc. were used as sterilized products, instruments including the culture bottles were used after autoclaving for 15 minutes at 121 ℃, 15 1b in an autoclave. The medium was dissolved in tertiary distilled water and then sterilized by sterile filter (pore size 0.22 μm) and used by adding 10% fetal bovine serum and 1% streptomycin-penicillin. The temperature was maintained at 37 ° C. during cell wash and passivation.

When cells grew to adhere to the bottom of the culture flask, the attached cells were separated and used. Cells were cultured in a CO ₂ incubator while maintaining the conditions of 37 ° C. and 5% CO ₂ in a culture flask containing DMEM, respectively. For anchorage-dependent cells, the medium was removed and treated with trypsin-EDTA (0.05% trypsin, 0.53 mM EDTA.4Na) at 37 ° C. for 5 minutes. After separating these cells, centrifugation was performed at 1000 rpm for 5 minutes, the supernatant was removed, and the process was repeated three times. The cells thus obtained were dispersed in a medium and used at a constant cell number. Freezing medium was added to the cells and stored in a -70 ° C liquid nitrogen tank, and the cells were thawed and cultured immediately before use.

(2) reactive nitrogen species secretion test

Samples and strain RAW264.7 (2 × 10 ⁵ ) were placed in 96-well plates, and then incubated for 48 hours at 37 ° C. in a CO ₂ incubator. Nitrite concentration in the culture was measured using a microplate assay. First incubate for 48 hours, then take 50 μl of supernatant, and add 2.5 mL of the same volume of Greases reagent [1% sulfanilamide / 0.1% naphthylethylene diaminc dihydrochloride]. % H ₃ PO ₄ solution] was added and then reacted at room temperature for 10 minutes. A standard curve was obtained by measuring absorbance at 540 nm using an enzyme immunoassay (ELISA) reader (Molecular Device, USA) using sodium nitrite as a standard for nitrite, and using Examples 1 to The content of reactive nitrogen species secreted from the cells was determined due to the composition prepared in 4.

(3) interleukin-1α secretion ability

RAW264.7 (2 × 10 ⁵ / well) macrophage cell lines and samples were placed in 96-well plates and incubated for 48 hours at 37 ° C. in a CO ₂ incubator following the same procedure as for measuring reactive nitrogen species secretion. The supernatant was taken and macrophage secreted interleukin-1α (IL-1α) was measured using an enzyme-linked immunosorbent assay (ELISA) according to the procedure shown in FIG. Specifically, blocking reagents were added to prevent nonspecific reaction of the antibody to microtiter plates coated with anti-mIL-1α monoclonal antibody, followed by three washes with wash buffer. 50 μl of biotinylated anti-mIL-1α was added to the washed plate, and 50 μl of standard IL-1α or cultured sample solution was injected into a well, followed by 2 at 25 ° C. Time incubation. The cultured plate was washed three times again with a washing buffer, and 100 µl of horse reddish peroxidase, which was streptavidin-conjugated, was added thereto, and then reacted at 25 ° C. Wash three times with wash buffer. 100 μl of the substrate (3,3 ′, 5,5′-tetramethylbenzidine) was added thereto, followed by color development at 25 ° C. for 30 minutes, and then 100 μl of 1N sulfuric acid was added to stop the reaction. This was measured by absorbance at 450 nm using an enzyme immunoassay reader (ELISA reader, Molecular Device, USA) to obtain a standard curve, and from the cell due to the composition prepared in Examples 1 to 4 using this curve. The secreted IL-1α content was determined.

(4) tumor necrosis factor (TNF) secretion ability

To confirm whether the sample enhances tumor necrosis factor (TNF) production of macrophages, the same procedure as in IL-1α secretion assay was performed, and TNF secretion was analyzed using enzyme immunoassay (ELISA). Specifically, 50 μl of anti-mTNF attached with standard TNF or cultured sample solution and tin to the wells of the microtiter plate was biosynthesized and incubated at 25 ° C. for 2 hours. The cultured plates were washed five times with washing buffer, 100 μl of HRP-conjugated anti-mTNF was added, and then incubated at 25 ° C. for 30 minutes. Wash five times with wash buffer. 100 µl of the substrate (3,3 ', 5,5'-tetramethyl benzidine) was added thereto, followed by color reaction at 25 ° C for 30 minutes, and then 100 µl of 1N sulfuric acid was added to stop the reaction. The absorbance at 450 nm was measured using an enzyme immunoassay (ELISA) reader to obtain a standard curve, which was used to calculate the TNF content secreted from the cells due to the compositions prepared in Examples 1-4.

Table 5 below shows the immune activity of the milk composition of the tablet form prepared in Examples 1 to 4, determined according to the above procedure.

Table 5

Claims (3)

Regarding the total weight of the composition excluding the weight of the hydroxypropylmethylcellulose coating, from 50 to 70% by weight of skim milk powder, 3.0 to 7.0% by weight of prickly pear extract powder, 1.0 to 3.0% by weight of Deodeok extract powder, mushroom mushroom extract powder or mushroom 1.0 to 3.0% by weight of mushroom powder, 1.0 to 3.0% by weight of mushroom extract or mata mushroom powder, 0 to 10.0% by weight of colostrum powder, 0.5 to 2.0% by weight of ganoderma lucidum extract powder, 0.5 to 2.0% by weight of mushroom extract powder %, Ginseng powder 3.0-7.0 wt%, lactoferrin 0.2-1.0 wt%, glutamine peptide 5.0-15.0 wt%, lactose 1.0-5.0 wt% and crystal oligosaccharide 1.0-3.0 wt%, hydroxypropylmethylcellulose on the surface Milk composition in the form of a tablet having a coating to enhance immune activity. delete (1) Prickly Pear Extract Powder, Deodorant Extract Powder, Mushroom Mushroom Extract Powder or Mushroom Mushroom Powder and Matana Mushroom Extract Powder or Mater Mushroom Powder, Colostrum Powder, Ganoderma Lucidum Extract Powder, Situal Mushroom Extract Powder, Ginseng Powder, Lactoferrin Adding at least one component selected from the group consisting of glutamine peptides, lactose, crystalline oligosaccharides, and mixtures thereof, and mixing with a Hobart blender at 10 to 20 ° C. for 20 to 60 minutes, (2) adding 50 to 70% by weight of skim milk powder to the mixture obtained in step (1), based on the total weight of the composition excluding the weight of the hydroxypropylmethylcellulose coating, and mixing the mixture uniformly; (3) aging the mixture obtained in step (2) at 5 to 15 ° C. for 2 to 5 hours, (4) preparing the aged mixture obtained in step (3) in the form of a tablet (5) coating the tablet obtained in step (4) with a 3% solution of hydroxypropylmethylcellulose, A process for preparing a milk composition in the form of a tablet according to claim 1.

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