KR100650508B1 - Drinking water constituent for domestic animals and its preparation method - Google Patents

Abstract

A drinking water constituent for domestic animals and a method for manufacturing the same are provided to improve disease resistance and prevent diseases by using components of artemisia capillaris and matters eluted from microorganisms, and improve the quality of meat while stably managing breeding. Gathered artemisia capillaris is cleaned and dried. The dried artemisia capillaris is crushed into certain sizes. Water is added to the artemisia capillaris. The water-added artemisia capillaris is double-boiled to obtain double-boiling extracted water. 4.5 % of a mixture of thee kinds of lactobacilli, lactobacillus acidophilus, lactobacillus casei, lactobacillus platarum, which are not affected by artemisia capillaris in rearing, and 0.5% of rhodopseudomonas spp. are added to the double-boiling extracted water. The mixture is anaerobically fermented in a constant temperature room at 30 degrees for two days. The mixture is stayed at a normal temperature for one day to stabilize microorganisms. The mixture is wrapped in a sterilized container to prevent secondary pollution.

Description

Drinking water constituent for domestic animals and its preparation method

1 to 9 are photographs showing the culture results of the microorganisms to obtain a microorganism used in the present invention,

10 is a manufacturing process chart of drinking water for livestock according to the present invention,

11 is an essential oil gas chromatogram of mugwort extract and ethanol extract of wormwood,

12 to 16 are photographs showing the results of growth effects of mugwort extract on E. coli, Salmonella, Staphylococcus aureus, B. subtilis and B. lichenifomis .

The present invention relates to drinking water for livestock and a method of manufacturing the same, and more specifically, after washing the collected mugwort to prevent the inflow of foreign substances during the water bath process of lion foot wormwood and dried so that the moisture content is 10 to 20%, After crushing to a certain size in order to extract the useful substances of the wormwood more easily, put water 10 times the weight of lion foot wormwood by the hot water extraction method, and heated for 24 hours to obtain the hot water water extract, and by the wormwood in the water extract 4.5% of Lactobacillus acidophilus , L. casei , L. platarum lactobacillus and Lhodopseudomonas spp. Unaffected by growth And 0.5% of the mixture was added to the fermentative anaerobic fermentation in a constant temperature room at 30 ° C. for 2 days, and then allowed to stand at room temperature for 1 day to stabilize microorganisms. Preparation method of animal drinking water formulations, characterized in that packed in sterilized containers to prevent the salt and a process for the preparation of drinking water for livestock production in the above-described way.

Mugwort is a perennial plant belonging to the Asteraceae family, and it is widely distributed in Korea, Japan, and China. Mugwort is known to be effective against dozens of diseases by pressing white bottle and controlling all instruments. Because of its taste, it has been used as a material for rice cakes and rice cakes such as songpyeon and slices. In particular, wormwood clears blood and helps digestion, and it is also effective for skin beauty. Attempts have been made to add to a variety of foods.

The unique scent of mugwort is due to the essential oil component of cineol, which stimulates the secretion of digestive juices to help digestion after meals, and the bitter taste of bittersweet is a glutinous ingredient.

In particular, Ganghwa lion foot wormwood grows in the foot of 100m above sea level of Ganghwa Beach, which is blessed with oily land, sea breeze and sea breeze, and the effect of Ganghwa lion foot wormwood is described in the Dangun myths of Samguk Yusa. Mugwort ”, Heo-Jun's consent includes nutrients (outpost-carotene, leaf-vitamin A, B, C, root-arteus) that have different effects on leaves, roots, and stems to be effective as various medicines for women's diseases. It is included in the world of oriental medicine and is recognized as one of the world's best. In Dongguk Yeoseungseung and Herbology, it is recorded as a special product of Ganghwa Island.

Mugwort is not poisonous, it has been known to ward off the bad energy and customs of the five intestines, and it is good to use it as a folk remedy for colds as well as to bleeding or bleeding. In addition to the wormwood has been used a lot as a hemostatic drug such as nosebleeds, uterine bleeding in oriental medicine, it is effective in digestion, pain in the lower abdomen, elimination of hookworms and odors, and is effective in gastrointestinal diseases, constipation, neuralgia, cold sickness, women's diseases and asthma It has been drinking as a mugwort tea.

Mugwort is rich in amino acids such as threonine, tryptophan, valine, alanine, and leucine, which can be used as food resources.Alkali is the main ingredient. , Essential oils, various minerals, vitamins, etc. are known, the main nutritional ingredients of mugwort are 5.2g protein, carbohydrate 10.6g, calcium 93mg, phosphorus 55mg, iron 10.9mg, vitamin A 7940I.U. Vitamin C is 20mg, which is rich in minerals and vitamins. Vitamin A is known to contain twice as much carrot as it is.

Calories (Kcal) moisture (%) Protein (g) Lipid (g) Carbohydrate (g) Fiber (g) Ash (g) Ca (mg) Fe (mg) Vt.A (I.U) Vt.B (mg) Vt.C (mg) 56 81.4 5.2 0.8 4.0 3.7 2.0 93 10.9 7940 0.44 20

Essential oil is a mixture containing a large number of fragrance substances in a certain ratio, a single component has a low pharmacological effect, but a mixture of more than 65 kinds of compounds showing great efficacy by mutual inhibition and edification of several components, lion foot mugwort The essential oils contained in it are cineol, tricyclene, bornol, borneol, thujone, terpnene, terpine, etc. Aureus ( Staphylococcus aureus), a Streptococcus Sangu devices cause of dental caries (Streptococcus sanguis ), Streptococcus mutans (S. mutans), Escherichia coli (E. coli), In recent years, many studies have shown that there is an antimicrobial effect of inhibiting pathogenic microorganisms such as Salmonella , as well as pharmacological characteristics such as increased immunity, nervous system stability, anticancer effect, anti-aging and antibacterial activity against skin diseases. It has been.

Artemisia Capillarisin and 6,7-dimethylesculetin contained in capillaris have the effect of promoting the secretion of bile acids, lipolytic enzymes secreted by the liver, and in the dimethyl contained in buds. Sureretin has been shown to protect the liver, and water-soluble extracts have been reported to improve glucose metabolism.

Mugwort extracts were found to have vasorelaxant effects on blood pressure using rats and rabbits, and the effects of mugwort, dermis, and larvae on lipid metabolism in rats with hyperlipidemia induced by high fat diet. It was known that the total lipid concentration in blood showed a tendency to decrease in mugwort and dermis treatments compared to the control and larvae treatments. Also, Artemisia iwayomogi Kitamura ) The effects of ethanol extracts on rat liver, serum cholesterol and lipid metabolism decreased serum cholesterol and triglycerides, suggesting that wormwood extract promotes lipid metabolism in liver. The extract has the effect of activating the tumor necrosis factor, and has been reported to have an anti-tumor effect by direct cytotoxicity, and in the experiments using rats, cineol is known to have a chemotherapy effect on breast cancer.

Mugwort with a variety of efficacy as described above is used only for a specific use, the situation is not to use a large number of native wormwood is left unattended, the development of drinking water for livestock has not been attempted.

Therefore, an object of the present invention is to improve the anti-history and disease prevention effect by the effects of substances eluted by microorganisms and microorganisms in addition to the essential oil components of mugwort, and to facilitate the production of volatile fatty acids in the rumen, the energy utilization of feed intake It is possible to improve oil quality even though it is weak by vitamin A which is contained in very small amount, and it is possible to manage stable breeding by the function of wormwood and the effect of useful microorganisms, and diarrhea or hariation due to pollution in poor environment after birth. To provide a drinking water preparation for livestock that can be prevented.

In other words, by drinking water-reinforced lion foot wormwood to increase the utilization of useful functional substances contained in the mugwort, and by adding useful microorganisms and fermentation to increase the effect, not only the effects of functional substances, but also the action of intestinal action and harmful bacteria It is an object of the present invention to provide a drinking water preparation for livestock, which is capable of improving weight gain and improving meat quality by enhancing inhibition and digestive absorption rate.

Another object of the present invention is to provide a method for producing a drinking water preparation for livestock of the above object.

In order to achieve the above objects as well as other objects that can be easily expressed in the present invention, after washing the collected mugwort in order to prevent the inflow of foreign substances during the water treatment process of lion foot wormwood and dried so that the moisture content is 10 to 20% After crushing to a certain size in order to extract the useful substances of the wormwood more easily, put water 10 times the weight of lion foot wormwood by the hot water extraction method, and heat the water for 24 hours to obtain the hot water water extract. Lactobacillus acidophilus , L. casei , L. platarum lactic acid bacteria and Lhodopseudomonas spp. 4.5, which are not affected by growth, % And 0.5% were added, and the fermented anaerobic fermentation was carried out for 2 days in a constant temperature room at 30 ° C. In order to prevent secondary contamination, the preparation of drinking water preparations for livestock is carried out by packing them in sterilized containers and diluting them with water for drinking to livestock, so that the substances eluted by microorganisms in addition to the essential oil components of wormwood and the microorganisms themselves. It is possible to improve the anti-medical history and disease prevention effect, to facilitate the production of volatile fatty acids in the rumen, improve the energy utilization of feed intake, and to improve the oil quality of the small amount by the very small amount of vitamin A. Due to its functionality and useful microbial effects, stable breeding management was possible, and diarrhea and hariation caused by contamination in poor environment after birth were prevented.

The present invention is described in more detail as follows.

Method for producing a drinking water preparation for livestock according to the present invention, after washing the collected mugwort to prevent the inflow of foreign substances during the water bath of lion foot wormwood, dried to 10 to 20% water content, and then easy to use the useful material of the wormwood After crushing to a certain size in order to extract the water, add 10 times the weight of lion foot wormwood weight by the method of water extraction and heat the water for 24 hours to obtain the water extraction, and lactose which is not affected by the growth of the water by wormwood in the water extraction. Lactobacillus acidophilus , L. casei , L. platarum lactobacillus 3 liquid mixture and photosynthetic bacteria ( Rhodopseudomonas spp. ) Were added to 4.5% and 0.5% After anaerobic fermentation for 2 days in a constant temperature room at 30 ℃, it was allowed to stand at room temperature for 1 day for stabilization of microorganisms and then sterilized to prevent secondary contamination. It characterized in that the packaging.

In the present invention, three types of raw mugwort, one year dried mugwort, and three years dried mugwort were used as the mugwort as the potted lion's foot, and the mugwort was the lion's foot of the mugwort collected from October 12, 2005 in Ganghwa-gun. ㎝ was dried and stored in the shade for 1 day after washing, and dried one-year and three-year old wormwood was purchased from phosphate foods in Ganghwa-gun.

The general components of each type of lion foot wormwood were analyzed by the Science and Technology Analysis Center, and the results are shown in Table 2.

Microorganisms and fungi rDNA isolated from MRS agar, Nutrient agar and YM agar for use in the present invention The nucleotide sequences were analyzed, and the analyzed nucleotide sequences were compared for similarity by NCBI blast search, and the research institute was conducted at the Korea Institute of Science and Technology.

As a result of identifying and identifying some of the most visible microorganisms and fungi isolated from mugwort extract, seven microorganisms and three fungi were identified. That is, as can be seen from Table 3, among the seven microorganisms, there were five kinds of lactic acid bacteria (Table 3; No. 1 to 7), two Bacillus species (Table 3; No. 8 to 9), and in the soil As a result, one of the microorganisms (Table 3; No. 9) was found, and about 4 types of fungi were detected by extracting mugwort extract and fungi that may occur when drying mugwort. , No. of Table 3 It confirmed that it was 10-12.

Mugwort-derived microorganisms and fungi  No Label Simil. top BLAST Sim One AJ2 Lactobacillus sakei  97% 687/690 2 AJ3 Lactobacillus sakei  99% 777/778 3 AJ6 Lactobacillus plantarum  99% 777/778 4 AJ8 Lactobacillus plantarum 100% 758/758 5 AJ7 Weissella cibaria 100% 749/749 6 AJ9 Pediococcus pentosaceus  99% 773/774 7 AJ5 Serratia marcescens  99% 776/777 8 AJ1 Bacillus pumilus  99% 773/776 9 AJ4 Bacillus cereus  99% 782/784 10 F1 Eupenicillium tropicum 100% 255/255 11 F3 Penicillium sumatrense 97% 521/534 12 F4 Neurospora intermedia 98% 552/558

On the other hand, whether the microorganism is affected by the growth by the antimicrobial effect of the previously known mugwort, and the microorganisms are not affected by the growth was used in the present invention.

In other words, the extract water used in the experiment was a stock solution that was not sterilized with a bath water extract or a compressed extract water, and sterilized water at 85 ° C. for 30 minutes, and sterilized water at 121 ° C. for 15 minutes. , After spreading the microorganisms in agar plate medium, the paper disk (paper disk) was raised and loaded with the respective extracts (loading) and dried, and then cultured for 2-3 days and observed. The microorganisms listed in Table 4 are Lactobacillus acidophilus KCTC 3164, L. casei KCTC 2180, L. platarum KCTC 3108 as yeast, and known strains. Mises S. cerevisiae KCTC 7286, B. subtilis KCTC 3560, Rhodopseudomonas capsulata KCTC 2583 as photosynthetic bacteria, Streptomyces griceus as actinomycetes KCTC 1072 was used.

Microorganisms and Culture Media microbe badge Lactobacillus Lactobacillus acidophilus MRS  L. plantarum 〃  L. casei 〃 Enterococci Enterococcus faecium 〃 leaven Saccharomyces cerevisae 〃 photosynthesis Rhodopseudomonas palustris R ₂ A Bacillus Bacillus subtilis Nutrient  B. lichenifomis 〃 Actinomycetes Streptomyces griceus R ₂ A

In most cases, lactic acid bacteria, yeast, enterococci, photosynthetic bacteria, etc. did not appear to have a significant effect on growth, but actinomycetes and Bacillus had a slight effect on the overall growth, which is inferred from the inhibition of growth by essential oils. Actinomycetes were affected, but not only added a very small amount in the production of drinking water for livestock, but the added amount also plays a sufficient role.In case of Bacillus, the influence of Bacillus spp. As a result of the experiment, subtilis was affected very finely, and Rikenimoform was slightly affected (FIGS. 1-9).

In the present invention, the extraction of the reinforced lion foot wormwood was used a hot water extraction method or compression extraction method. That is, the boiling water extraction method is a double heating form, the temperature of the external water is 100 ℃, the temperature of the internal water is about 85 ℃ and the heating method for 24 hours, the extraction water has a bitter scent like the traditional herbal medicine, Taste can also feel bitter taste, can obtain a water extract, which is a relatively clear dark brown liquid without debris, the compression extraction method by the pressure of both teeth to crush the thick stem and leaves at once It has the advantage of extracting water, and it has a rich mugwort scent like the bath water extract, but the obtained extract water is a turbid dark green extract water in the form of a lot of suspended solids and must be mixed uniformly during the preparation or use of the preparation for drinking water. And, there is a drawback that the first pollution occurs by the microorganisms that were together with the wormwood residues.

On the other hand, various lactic acid produced by lactic acid bacteria (lactic acid, acetic acid, benzoic acid) acidify the pH to neutralize pathogenic bacteria, restore the intestinal flora to normal to prevent diarrhea and enteritis, and hydrogen peroxide produced by lactic acid bacteria to the cells Bacteriocin, an antibiotic produced by lactic acid bacteria, inhibits the growth of decay-producing bacteria. Therefore, lactic acid bacteria maintain the normal bacterial flora in the intestine, inhibit pathogenic microorganisms, and in particular, by preventing the enteritis and hari the effect of improving the animal's growth rate and feed efficiency, according to the present invention three different lactic acid bacteria Lactobacillus ash Lactobacillus acidophilus ), Lactobacillus catase ( L. casei ), and Lactobacillus platarum ( L. platarum ) were used.

In addition, the photosynthetic bacteria used in the present invention are Gram-negative bacteria, and 65% of the cell components are composed of proteins, and contain a high content of essential amino acids and vitamins, with high value as a nutrient and hydrogen under anaerobic conditions. It has the property of using and carbon to reduce the methane gas produced during ruminant digestion.

In general, the inoculation amount during inoculation of microorganisms should not exceed 2 ~ 5% of the total dose. The present invention also inoculated and cultured with lactic acid bacteria and photosynthesis to be 3%, 5%, 10% of the total mugwort juice, all were maintained at 10 ⁷ cfu / ml or more in the number of microorganisms. Although it was not the original culture medium in cultivating microorganisms, it was possible to grow microorganisms smoothly because the microorganisms made full use of large amounts of nutrients contained in wormwood, but more than 35% of soluble sugars contained in wormwood existed. The inoculation amount was determined to be 5% in consideration of the microbial titration medium.

Drinking water formulations for livestock of the present invention are prepared by the following method.

That is, since foreign matter may be introduced during the lion's foot wormwood treatment, it is preferable to dry the collected wormwood to 15% moisture content after washing, and to crush it to a certain size in order to extract the useful substance of the wormwood more easily.

Then, add water 10 times the weight of lion foot wormwood by the water extraction method and heat it for 24 hours.

The hot water extraction method has the advantage that it can be used immediately without a separate sterilization process for the preparation of the preparation for drinking water in the state of the first sterilization by heating, there is an effect of reducing the cost of manufacturing the preparation for drinking water than the compression extraction method. In addition, it is possible to use the extracted water in a uniform state without sediment, so that the bath extraction method is adopted to minimize the problems that may occur during manufacturing or product use. In the case of the compression extraction method, the supply and demand of raw materials is not smooth due to seasonal factors, and storage and storage are not easy even when they are compressed and extracted in large quantities. Therefore, it was selected as a hot water extraction method that can store raw materials in a dry state.

4.5% lactic acid bacteria and 4.5% and photosynthetic bacteria were added to the extracted water obtained by the method using water bath so that 4.5% and 0.5% of lactic acid bacteria were added. Keep it stagnant. It is desirable to package in sterilized containers to prevent secondary contamination after manufacture.
The drinking water preparation for livestock prepared by the above method is added to 1 to 3% by weight to the water used as drinking water for ordinary livestock and used as drinking water for livestock.

The following Examples, Comparative Examples, and Experimental Examples illustrate the present invention in more detail, but do not limit the scope of the present invention.

Example  One

For the development of drinking water for livestock using mugwort extract, the compressed extract was fermented. Put the prepared microorganism in sterilized or unsterilized mugwort extract without putting anything in the hardened mountain lion foot wormwood extract. The microorganisms were Lactobacillus acidophilus , L. casei , L. platarum lactic acid bacteria 4.5% mixture, photosynthetic bacteria ( Rhodopseudomonas) spp . ) 0.5% and ferment for 3 days in constant temperature room (30 ℃). Mugwort extract water was used in the experiment by sterilized extract stock solution (Table 5, # 1), 85 ℃ 30 minutes sterilization (Table 5, # 2), 121 ℃ 15 minutes sterilization (Table 5, # 3), inoculation The microorganisms used at maintained at 10 ⁷ cfu / ml. After fermentation for 2 days, taken out, through the stabilization step for one day, the pH and fermentation analysis, microbial number was confirmed on the fifth day and one month, and are shown in Table 5. The control is sterile mugwort extract without microorganisms (Table 5). The medium used to observe the microorganisms is MRS agar with bromcresol purple, Nutrient agar, and R ₂ A agar media.

As can be seen from Table 5, in the control group without the microorganisms, it was confirmed that after a time, other microorganisms that grew variously with the flavor modified in the original mugwort extract water. This shows that the microorganisms with the original wormwood were grown or contaminated microorganisms remained during the compression extraction process of the extract, and the deformation may occur if the extract is stored for a long time.

In the case of extract water fermented with microorganisms, the rich aroma of mugwort was softened, and it was found to be changed into a sweet aroma due to lactic acid bacteria. The sterilized # 1 extract was not fermented on day 5, but the wormwood was fermented well, but changed over time. Sterilized at 121 ℃, # 3 extract had the strongest color after sterilization and had the strong bitter scent that can be handled by herbal medicines. It was confirmed that the reduction to about half. The best results were # 2 sterilized at 85 ° C for 30 minutes and then fermented extract water showed a steady condition after one month in the fragrance and microbial counts. Therefore, in the case of using the compressed extraction water, it was most effective to sterilize at 85 ° C. for 30 minutes.

Example  2

The sterilization was carried out in the same manner as in Example 1 except that the water extract was used, but the sterilization was not carried out. Therefore, the control was an extract without microorganisms and fermented at 30 ° C. for 2 days. One experiment was observed and the results are shown in Table 6.

Experimental Example  One

The essential oil components of the water extract and the fermented extract were extracted and analyzed to compare and analyze the substances produced or increased after fermentation by gas chromatograph (GC) and GC-Mass spectroscopy (GC-MS).

The essential oil component extraction from the extract water was carried out in the following manner.

Add 1/2 vol. Of dichloromethane to the water extract 525 ml, and shake it for 15 hours at 30 ° C. Centrifuge the mixed samples for 15 minutes at 14,000 rpm, and recover only the lower layer of dichloromethane using a separatory funnel. The supernatant is discarded. 200 g of NaSO ₄ is added and left for 24 hours to completely remove any remaining moisture, and then NaSO ₄ is separated by vacuum filtration. The remaining dichloromethane is removed using a rotary vacuum evaporator to obtain 0.03 g of an essential oil component, the extracted essential oil component is dissolved in 4 ml of dichloromethane, and the prepared sample is subjected to gas chromatography for analysis. The analysis conditions are as follows.

Hewlett-Packard (HP) 5890 GC

DB-Wax bonded phase fused silica capillary column: 30m × 0.25mm (d = 0.25)

Injector and flame ionization detector: 250 °

Temperature program: Increase to 40 ° C in 2 minutes and 200 ° C to 2 ° C / min

Linear velocity He carrier gas: 30 cm / s

Data output: HP Chemstation

The result of comparing the essential oil components obtained from the extracted water and the fermented extract water is shown in Figure 11, the upper graph is a bath water extract and the lower graph is the extract water after fermentation. The decrease or disappearance of the concentration after fermentation is indicated by a red line, whereas some substances that appear or increase in fermentation extract water are indicated by a green line. As a result of the analysis of GC-MS with four substances found both in the stock solution and after fermentation, eucalyptol, camphor and borneol, which are detected in essential oils of mugwort, were also observed. As a substance with decreasing concentration, No. 1, 2, 5, 6, 18, 11 substances produced or increased after fermentation. 4, 7, 13, 22, and 23, most of them were antioxidants, antimicrobial, and immunosuppressive substances were found. Eucalyptol decreased significantly after fermentation, while cis-Jasmone and Salvialane increased relatively.

GC-MS Analysis of Essential Oils from the Extracts of Boiled Water and Fermented Water No . RT ( min ) Compound formula Mol wt One 11.5  Eucalyptol C ₁₀ H ₁₈ O 154 Improving Immunity to Bronchial Asthma 2 24.8  1-Octen-3-ol C ₈ H ₁₆ O 128 3 27.6  Camphor C ₁₀ H ₁₆ O 152 Very effective antioxidant 4 37.0  (+) alpha-Terpineol C ₁₀ H ₁₈ O Antioxidant, antibacterial effect 5 38.3  Borneol C ₁₀ H ₁₈ O 154 Antioxidant, antibacterial effect 6 38.7  Alpha-Terpineol C ₁₀ H ₁₈ O 154 7 40.1  Gamma-Campholenol C ₁₀ H ₁₈ O 154 13 50.7  cis-Jasmone // 2-Cyclopenten-1-one C ₁₁ H ₁₆ O 164 Vegetable pest control 18 68.5  Methyl jasmonate C ₁₃ H ₂₀ O ₃ 224 22 81.3  Salvialane C ₁₅ H ₂₈ 208 23 81.8  Pyrocatechol // 1,2-Benzenediol C ₆ H ₆ O ₂ 110 Antioxidant Activity and Fat Stabilization with Catechin Derivatives

Experimental Example  2

Representative effects of vitamin A include decreased mastitis, decreased somatic cell count and improved fertility rate. Proper administration of vitamin A and beta-carotene to cows increases the resistance to bacteria and prevents mastitis, whereas deficiency reduces the ability of keratin and immunoglobulins to make bacteria easier to multiply after invasion. Bacteria are known to be able to invade the mammary gland and develop full-scale mastitis.

Recently, Hwa beef farmers are trying to improve the meat quality due to vitamin A deficiency. The vitamin A level in the body changes depending on the growth stage. Vitamin A deficiency until 30 months of age is good but the body weight is poor and the symptoms are likely to appear deficiency. Therefore, in order to simultaneously improve weight gain and meat quality, it is recommended that vitamin A be fully supplied to the growing and finishing seasons (25 to 30 months of age) and deficient in finishing meats.

As such, the level of vitamin supplementation should be adjusted according to the growth stage. In the growing stage, vitamin A should be sufficiently supplied in advance in order to develop the rumen in preparation for the adverse environmental conditions such as multi-dense rich feed in the growing season. However, it is important to note that overdose increases the amount of accumulation in the body, which can make vitamin A deficiency difficult during periods of strong intramuscular fat accumulation. During the finishing period, vitamin A additives are excluded, and supplementation with beta-carotin and low vitamin A content is recommended, and at the end of the finishing period, vitamin A preparations are orally administered or added to feed.

Vitamin A supplementation by growth stage is administered orally or intramuscularly three times (2 to 3 million IU / once) once every 9, 11 and 13 months of age in the growing season (9 to 15 months of age). Only IU should be added to feed. At the age of 20 to 25 months, 2,000 IU should be added to the feed daily, and at 25 to 30 months of age, 5,000 IU should be added to the feed daily, or 1 million IU orally or twice a day at 25 and 27 months of age. Is reported.

Therefore, by checking the content of vitamin A known to be contained in a large amount in mugwort, it was confirmed whether there is a function for the supply of vitamin A of drinking preparations for domestic animals.

Β-carotin content of fermented mugwort sample β- carotin  (Μg / 100g)  Fermented Wormwood  55.57  Fermented Extract Extract  461.17  Fermented Water Extract  3.96

Vitamin A 1IU multiplied by beta carotene (β-carotin: ㎍) 0.3 times the amount of vitamin A in fermented mugwort 16.67 IU, 138.35 IU, 1.19 IU, the highest amount in the pressurized extract water. The water extract of the water bath is a process of extracting water from the water bath, and vitamin A, a fat-soluble vitamin, was not extracted as much as expected.

During the growing season, the rumen develops and the body growth is active. Therefore, vitamin A must be supplied enough. Therefore, the calf is fed at the time of calf introduction, and the feed with high vitamin A content is maintained to maintain the plasma concentration above 100 IU / dl. It is preferable to make it good, and the vitamin A content of the drinking water for livestock of the present invention is very difficult to expect the effect of vitamin A in a small amount, but if the calf feeds up to foster electricity, it can help to improve the quality of the meat.

Experimental Example  3

Carbohydrates in the rumen is and is fermented by microorganisms producing volatile fatty acids (VFA), the main volatile fatty acids see it listed a number of unique acetate, propionate (propionate), butyrate (butyrate), isobutyl butyrate (iso - butyrate), Bali rate (valerate), isopropyl Bali rate (iso - valerate) and the other a small amount of acid. These volatile fatty acids are absorbed into the ruminant's body, providing 80% of their energy needs.

Acetate accounts for 50-60% of total volatile fatty acids and is produced when fiber is fermented, and is absorbed into the body to be used for fatty acid synthesis and fat synthesis in adipose tissue. To supply.

Propionate accounts for 18-20% of total volatile fatty acids, and the production rate increases with increasing feed. After absorption, it is converted into glucose in the liver and used as an energy source or for the synthesis of lactose in milk.

Butyrate accounts for 12-18% of total volatile fatty acids and is mainly converted to ketones (beta-hydroxybutyrate (β-HBA)) in fatty tissues and breasts in the primary epithelial cells during absorption. Used for the synthesis of

The fermentation state in the first phase is also evaluated by the production rate of acetate and propionate. In general, under optimal fermentation, the ratio of A to P should be maintained at 2.1: 1.

The results of the comparison of the volatile fatty acid contents of the water extract and the water extract after fermentation are shown in Table 9. The high ratio of acetate was due to the high content of cellulose, and it was confirmed that the amount of aceteite decreased after a fermentation in all items, including 66%. It was confirmed that the volatile fatty acid content of the extract water was reduced by the added microorganisms, and that the acetate content was the most advantageous and convenient for the growth of microorganisms. This stabilizes the ratio of acetate: propionate: butyrate to reduce the energy wasted as methane in the rumen.

Volatile Fatty Acid Content of Mugwort Extract Volatile Fatty Acid Water Extract Fermented Water Extract  acetate 113 74.7  propionate    5.2  4.2  butyrate    1.0  0.9 iso - butyrate    3.9  3.5 iso - valerate   19.2 16.4 valerate    0.7  0.6

Experimental Example  4

Mugwort extracts and fermented extracts obtained by using the water bath were tested for antimicrobial activity against pathogenic microorganisms.

The microorganism tested was Salmonella enterica subsp. enterica ATCC 43971), Staphylococcus aureus subsp. aureus ATCC 25923) and Escherichia coli ATCC 9637 were distributed from Korea Cell Line Bank, and microbial growth inhibition was observed using a disk method, and the results are shown in FIGS. 12 to 14.

The effects of mugwort extracts on three pathogenic microorganisms showed that neither the stock extracts of the mugwort nor the fermented extracts showed antimicrobial activity. Although it showed inferior antimicrobial effect only against staphylococcus. There was no significant result, and this is the antibacterial effect of essential oil contained in mugwort, and it is difficult to extract essential oils containing 0.5 ~ 1.4% by water extraction method.

Experimental Example  5

In order to determine the production rate and dilution ratio of the drinking water for livestock of the present invention to be prepared, the feed amount was determined using pH analysis and the degree of effect of bacillus, pathogenic microbial growth. The microorganisms tested were Bacillus ( B. subtilis , B. lichenifomis ), Salmonella ( S. enterica ), Staphylococcus ( S. aureus ), E. coli ( E. coli ) were performed.

As can be seen from Figures 15 to 16, diluting the stock solution of wormwood extract and fermented extract water to determine the growth inhibition of Bacillus and the growth inhibition of pathogenic microorganisms, Bacillus richenifomis ( B. lichenifomis ) And staphylococci only inhibited growth, but did not affect other microorganisms. In Bacillus licheniformis was more affected by the fermented extract than the stock extract, it was confirmed that the growth is inhibited at 10% dilution of the fermented extract. Staphylococcus aureus was slightly affected by extract water and 25% dilution after fermentation, but had no antimicrobial effect.

In addition, fermented mugwort extract has a low pH of 3.8. However, the best pH of digestion in the rumen is known as 5.8-6.3, and it is most preferable to dilute it with water (pH 6.8-7.2) in the ratio of 1 to 3%.

In addition, TMR feed (completely mixed feed) was fed to farmers in the Ganghwa area to evaluate palatability. As a result of investigating the intake behavior by adding 2%, 3%, and 5% of the preparation for drinking water to the negative reservoir of Korean cattle farms, they showed a strong curiosity about mugwort flavor. In the 5% addition group, the negative ingested cows were found to be rubbing.

As described above, in the present invention, extract water was prepared by the method of extracting the potted lion foot wormwood from the water bath, pH 3 through anaerobic fermentation by combining lactic acid bacteria and photosynthetic microorganisms that inhibit pathogenic microorganisms and help prevent enteritis and hari. In the production of drinking water for livestock of 8, the antibacterial effect was insignificant, but in the essential oils, the antimicrobial and antioxidative effects of alpha terpineol ((+) alpha-Terpineol), gamma-Campholenol, cis Functional substances such as cis-Jasmone were increased, and Campo was detected both before and after fermentation.Eucalyptol, Borneol, methyl jasmonate, etc. It was found that after fermentation, acetate in the VFA content was very high compared to propionate and butyrate, and the ratio was stable after fermentation. In the case of vitamin A, the content of vitamin A in mugwort is generally known to be about 7900 IU, but the vitamin A content of fermented drinking water for livestock fermented after fermentation of the medicinal herbs from Fortified Lion's foot is not significantly reduced. The results of the analysis show that, if the feeding period is fed from the calf to the breeding mechanism, the effect of improving the meat quality can be expressed. It improves the energy utilization of feed intake by facilitating the production of volatile fatty acids in the rumen, and it is possible to improve the oil quality by the very small amount of vitamin A, and by the function of wormwood and useful microorganisms Stable breeding management, poor environment after birth It could prevent diarrhea caused by contaminated or Hari standing.

Claims (3)

After washing and drying the collected lion foot wormwood, crushed to a certain size, put water in the lion foot wormwood by the water extraction method and heated it for 24 hours to obtain the water extract, and the growth of water by the wormwood in the water extract Lactobacillus acidophilus ( Lactobacillus acidophilus ), Lactobacillus catase ( L. casei ), Lactobacillus platarum ( L. platarum ) three kinds of lactic acid bacteria and photosynthetic bacteria ( Rhodopseudomonas spp. ) To be 4.5% and 0.5% Preparation of a drinking water preparation for livestock, characterized in that for two days of anaerobic fermentation in a constant temperature room at 30 ℃ and then to stand at room temperature for stabilization of microorganisms, and then packaged in a sterile container to prevent secondary contamination Way. The method of claim 1, wherein the drying of the lion foot wormwood is a method of producing a drinking water preparation for livestock, characterized in that the water content is 10 to 20%. Drinking water for livestock drinking, characterized in that the drinking water preparation for livestock prepared by the method of claim 1 or 2 was diluted with water to a ratio of 1 to 3% by weight.

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