KR100638055B1 - Cosmetic compositions containing belamcanda extracts - Google Patents

Abstract

The present invention relates to a cosmetic composition that inhibits the production of inducible nitric oxide synthase (iNOS), thereby inhibiting the production of nitric oxide (NO) and thus maintaining clean skin. The cosmetic composition according to the present invention contains the liver extract. The liver extract is an extract obtained by adding a mixed solvent of alcohol and water to the liver, and preferably, an organic solvent such as ethyl acetate, chloroform or dichloromethane and water are added to the extract obtained by adding a mixed solvent of alcohol and water to the liver. It is a partition extract obtained by separating the organic solvent fractions under reduced pressure and separating into a phase.

Liver extract, cosmetic composition

Description

Cosmetic composition containing beech extract {Cosmetic compositions containing belamcanda extracts}

Figure 1 is a graph showing the results of measuring the NO production inhibition of 80% ethanol hot water extracts and their distribution extracts between the dead.

The present invention relates to a cosmetic composition containing a liver extract, and more particularly, by inhibiting the expression of inducible nitric oxide synthase (iNOS) by various stimuli in skin cells (NO) It relates to a cosmetic composition containing a liver extract that inhibits the production of) to enable the maintenance of clean skin.

The skin of the human body is induced by various stimuli, i.e., parasites such as bacteria, physical stress such as cold, or mental stress, iNOS enzyme is produced and NO is produced by this enzyme, which leads to vasodilation. It has a negative effect on cell death and differentiation. Over-produced NO stimulates keratinocytes to cause inflammation, and also stimulates pigment cells (melanocytes) to overproduce melanin, making the skin black. In addition, NO also weakens the immune activity of Langerhans cells, which are immune to skin cells, and cause various diseases.

Meanwhile, Belancanda chinensis is a medicinal plant that has been used in combination with other medicines for various diseases in humans since ancient times. Has been reported, but no report has been reported on NO production inhibition.

An object of the present invention is to provide a cosmetic composition that can keep the skin clean by inhibiting NO production by inhibiting the expression of iNOS enzyme in skin cells.

The present inventors have completed the present invention by finding that the liver extract inhibits the production of NO, and their NO production inhibitory ability is due to the inhibition of iNOS enzyme expression.

The cosmetic composition according to the present invention contains the liver extract.

In addition to the known biological activity, soybean extract has a function of inhibiting NO production by iNOS enzyme (inducible nitric oxide synthase), which has recently attracted much attention. Do.

The liver extract in the cosmetic composition according to the present invention is an extract obtained by adding a mixed solvent of 95% to 10% of alcohol and 5% to 90% of water to the liver and heating at 85 ° C to 95 ° C for 3 to 5 hours.

In addition, the liver extract in the cosmetic composition according to the present invention is dried by evaporating and drying the extract obtained by adding a mixed solvent of alcohol and water to the liver, and then again by adding an organic solvent such as ethyl acetate, chloroform or dichloromethane and water. It is preferable to use a partition extract obtained by separating the organic solvent fractions under reduced pressure and then separating them into phases.

The partition extract of the soybean extract exhibited the inhibition of NO production by higher iNOS enzyme.

In the cosmetic composition according to the present invention, the amount of the liver extract is 0.05% by weight to 10.0% by weight based on the weight of the cosmetic, and in the case of the distribution extract, it is 0.005% by weight to 5.0% by weight based on the weight of the cosmetic.

The cosmetic composition according to the present invention may further include 0.025 wt% to 5.0 wt% of vitamin C or 0.025 wt% to 5.0 wt% of vitamin E with respect to the cosmetic weight.

In addition, the cosmetic composition according to the present invention may further include 0.025% to 5.0% by weight of vitamin C and 0.025% to 5.0% by weight of vitamin E with respect to the weight of the cosmetic.

Vitamin C and vitamin E are commonly used antioxidants in cosmetic compositions, where vitamin C is a water soluble antioxidant and vitamin E is a useful antioxidant. They act synergistically on the NO production inhibitory activity of the soybean extract.

Cosmetics containing the liver extract of the present invention has a formulation such as softening water, nourishing cosmetics, nutrition cream, massage cream, essence and pack.

Hereinafter, the present invention is described in more detail by some preferred embodiments. However, the present invention is not limited to the following examples.

Example 1

500 g of 80% ethanol was added to 100 g of barn, heated at 90 ° C. for 3 hours, and left at room temperature to obtain 80% ethanol hot water extract of barn. The extract was dried under reduced pressure to give 26.5 g of dried product.

Example 2

500 g of 80% ethanol was added to 100 g of bark, heated at 90 ° C. for 3 hours, and then left at 50 ° C. for 3 hours to obtain 80% ethanol hot water extract from bark. The extract was dried under reduced pressure to give 24.0 g of dried product.

Example 3

26.5 g of each of the ethanol hot water extracts obtained in the same manner as in Example 1 was partitioned into water, ethyl acetate, water and chloroform, water and dichloromethane, and dried under reduced pressure to obtain the following fractions.

Ethyl Acetate Fraction 6.2g-Water Fraction 15.8g

Chloroform fraction 4.8 g-Water fraction 14.2 g

Dichloromethane Fraction 2.9g-Water Fraction 16.2g

Example 4

24.0 g of each of the ethanol hot water extracts obtained in the same manner as in Example 2 was partitioned into water and ethyl acetate, water and chloroform, water and dichloromethane, and dried under reduced pressure to obtain the following fractions.

Ethyl Acetate Fraction 9.2g-Water Fraction 12.6g

Chloroform Fraction 8.0g-Water Fraction 10.2g

Dichloromethane fraction 2.2g-water fraction 18.0g

Example 5

RAW 264.7 cell lines were purchased from ATCC and cultured in the presence of 10% FCS in DMEM medium. 1 μg / ml of LPS was treated for 24 hours to express iNOS enzyme to produce NO. Thereafter, NO production was measured using a Griess reagent. As a result, the NO production amount of 2.8 ± 0.1 μM increased to 58.0 ± 4.5 μM (n = 3).

Example 6

In order to determine the NO production inhibitory ability of the extracts obtained in Examples 1 to 4, the same operation as in Example 5 was performed. The results are shown in FIG.

As can be seen in Figure 1, the liver extract inhibited NO production, among which the activity of the ethyl acetate fraction was high. In addition, when ethyl acetate fraction: vitamin C or E = 1: 1 or more, or ethyl acetate fraction: vitamin C: vitamin E = 2: 1: 1 or more, vitamin C or E was added to the ethyl acetate fraction. .

Example 7

Using ICR mice, the acute toxicity upon skin application and oral administration of the cosmetic composition according to the present invention was measured. As a result, the cosmetic compositions according to the present invention did not show acute toxicity up to 100 mg / cm ² in skin application and up to 1 g / kg in oral administration.

Prescription Example 1

A prescription example of the flexible cosmetics (skin) among the cosmetics containing the liver extract is as follows. Herein, the liver extract refers to Example 2.

ingredient Content (unit: weight%) Deadwood Extract 0.5 1,3-butylene glycol 5.0 glycerin 5.0 ethanol 10.0 Oleyl alcohol 0.1 Poly Sorbate 20 0.5 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount system 100

Prescription Example 2

A prescription example of nutrient lotion (lotion) in cosmetics containing soy extract is as follows. Herein, the liver extract refers to that of Example 2.

ingredient Content (unit: weight%) Deadwood Extract 0.5 1,3-butylene glycol 5.0 glycerin 5.0 Triethanolamine 1.2 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Solbitan Sesqui Olate 1.0 Carboxy Vinyl Polymer 1.0 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount system 100

Prescription Example 3

Prescription examples of nutritional cream among cosmetics containing soy extract are as follows. Herein, the liver extract refers to that of Example 2.

ingredient Content (unit: weight%) Deadwood Extract 1.0 Propylene glycol 5.0 glycerin 5.0 Triethanolamine 0.5 Liquid paraffin 10.0 Squalane 3.0 vaseline 7.0 Beeswax 2.0 Polysorbate 60 2.0 Solbitan Sesqui Olate 2.5 Carboxy Vinyl Polymer 0.5 antiseptic a very small amount incense a very small amount Pigment a very small amount Purified water Remaining amount system 100

As described above, the liver extract contained in the cosmetic composition according to the present invention inhibits the production of NO by iNOS enzyme, which is applied in the same way to skin cells. Therefore, the cosmetic composition according to the present invention inhibits the expression of iNOS enzymes by various stimuli in skin cells, particularly keratinocytes and fibroblasts, thereby inhibiting NO production and keeping the skin clean.                     

Claims (9)

Cosmetic composition containing the liver extract which is an iNOS enzyme expression inhibitor. The cosmetic composition according to claim 1, wherein the liver extract is an extract obtained by adding a mixed solvent of alcohol and water to the liver. delete delete The cosmetic composition according to claim 1, wherein the amount of liver extract is 0.05% by weight to 10.0% by weight based on the weight of the cosmetic. delete The cosmetic composition according to claim 5, further comprising 0.025% to 5.0% by weight of vitamin C based on the weight of the cosmetic. The cosmetic composition according to claim 5, further comprising 0.025% to 5.0% by weight of vitamin E based on the weight of the cosmetic. delete

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