KR20000044985A - Composition for prevention and treatment of dermatophytosis comprising extracts of belamcanda chinensis, aurantiilmmaturi pericapium, sanguisorbae radix, cnidii fructus and schisandra chinensis - Google Patents

Abstract

PURPOSE: A composition for prevention and treatment of dermatophytosis is provided which includes the plant extracts having antibiotic effects on trichophyton mentagro phytes and epidermophyton floccosum. CONSTITUTION: A composition includes 0.01-10 wt.% of more than one plant extract among Belamcanda chinensis, Aurantiilmmaturi Pericapium, Sanguisorbae radix, Cnidii fructus And Schisandra chinensis. The extracts is manufactured by the following steps: 1) grinding(10-200 mesh) dried plants, 2) extracting the grinded plants with one solvent among water, methanol, ethanol, propanol, butanol, glycerol, ethylene glycol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, benzene, hexane, diethyl ether, dichloromethane, and acetone, 3) filtering the extracts, 4) concentrating the extracts.

Description

Athlete's foot, eczema prevention and treatment composition

[Industrial use]

The present invention relates to a method for obtaining a plant extract from dead skin, green skin, fat milk, casualty, and Schisandra chinensis and a composition containing the extract. In particular, it inhibits the growth of trichophyton mentagrophytes and epidermophyton floccosum, which are known as the main causative agents of athlete's foot eczema, for preventing and treating athlete's foot eczema, which is effective in preventing and treating athlete's foot eczema. It relates to a composition.

[Prior art]

Athlete's foot is one of the most common symptoms of fungal infections. The causative agents of athlete's foot eczema are trichophyton rubrum, trichophyton metagrophyte and epidermophytone phlocosome, among the fungi (dermatophytes) having affinity for skin, and they penetrate keratin of skin and cause itching, redness, inflammation, etc. It is said to cause symptoms.

The exacerbation of these dermatophyte infections depends on the environment of the site of infection, the simultaneous infection of another opportunistic organism, and the individual's ability to immunize.

Closures by shoes, socks, etc. increase the heat and humidity of the affected area and promote the growth of dermatophyte.

According to Clin. Exp. Dermatol. 17, 1992, such athlete's foot eczema is more pronounced in adults than in young children, and 10 to 15% of the adult population of developed countries have athlete's foot eczema or have been infected.

In order to treat athlete's foot eczema, a method of killing the causative agent of athlete's foot eczema or inhibiting the growth of bacteria is considered. In this case, a keratin softener may be used together to remove stratum corneum where the athlete's foot fungus lives or to improve symptoms. Also, by washing and drying the affected area well, lowering the heat and humidity of the infected area to create an unfavorable condition for the growth of athlete's foot eczema may also help treat athlete's foot eczema.

As a treatment for athlete's foot eczema, antifungal agents and salicylic acid-prescribed external preparations, ointments, and sprays have already been developed and commercialized. However, athlete's foot is difficult to cure without recurring treatment without recurring symptoms.

Therefore, in order to completely treat athlete's foot eczema, it is necessary to continue treatment with antifungal agents even after symptom improvement to prevent re-infection.

Therefore, the object of the present invention has a specific antimicrobial activity against trichophyton mentagrophyte and epidermophytone procosome known as causative bacteria of athlete's foot eczema in consideration of the above problems and has a high safety for human body even after long-term use. Herbal medicines and plants were studied to find out that the liver, green skin, fat milk, casualty and schizandra extracts have excellent antimicrobial effects even at low concentrations against athlete's foot fungus. It is to provide a prophylactic and therapeutic composition.

[Means for solving the problem]

The present invention comprises 0.01 to 10% by weight of one or more plant extracts selected from the group consisting of dead liver, green skin, fat milk, casualties, and Schizandra chinensis to cause athlete's foot eczema tricophyton mentagrophyte and epidermomorph to achieve the above object. It provides a composition for preventing and treating athlete's foot eczema exhibiting an antimicrobial effect on ton flocosum.

Sagan, blue skin, fat milk, casualties, Schisandra chinensis extract is

a) pulverizing the plant medicament selected from the group consisting of dried loin, ground skin, fat milk, casualty and schizandra into powder of 10 to 200 mesh size with a grinder;

b) extracting the pulverized agent by cooling in a solvent for at least 48 hours;

c) filtering the extract; And

d) concentrating the filtered extract under reduced pressure

It is prepared by a method comprising a.

In addition, the extraction solvent is purified water, methanol, ethanol, propanol, butanol, glycerol, ethylene glycol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, benzene, hexane, diethyl ether, dichloromethane, acetone At least one selected from the group consisting of.

The invention is described in detail below.

The present invention relates to a mortar, blue skin, fat milk, casualty, Schizandra extract having an excellent antimicrobial activity against athlete's foot eczema causing bacteria and a composition for preventing and treating athlete's foot eczema containing these extracts.

Athlete's foot soap for preventing and treating athlete's foot according to the present invention can be applied to the drug at the same time as the washing of the infection site is easy to penetrate the drug in the infection site, and the hassle of the continuous drug application that is the most problematic when treating athlete's foot There is an advantage that can be solved.

The stem (belamcanda chinensis L.) used in the present invention is a root stem of an iris and a plant, which is a plant, and is dried in the sun by trimming fine roots. Known chemicals are belamcandine, iridine, tetolidine and the like, which have been used in the treatment of inflammation, tonsillitis and bad breath in Chinese and Korean medicine.

Citrus sp. Is the dried bark of the tangerine. Known chemicals are lemon acid, limonene, hesperidin and the like, and have been used in herbal medicine as an aromatic dry placebo and cough sputum medicine.

Fatty milk (sanguisorba officinalis L.) is a sun-dried root of the Root or Cucumber of the Rosaceae in spring or autumn. Known chemicals are tannins, acid sorbine, vitamin C, and the like, and have been used in the treatment of inflammation, oral inflammation and hemostasis in herbal and civil medicine.

Casualties (cnidiummonnier cusson or torilis japonica DC.) Are the fruit of the Asteraceae. Known chemical components are essential oils such as α-pinene and l-camphor, coumarin and the like, and have been used in the treatment of genital diseases and the like in Chinese medicine.

Schizandra chinensis baill is a sun-dried fruit of the Schizandra chinensis. Known chemicals are lemon acid, malic acid, and other organic acids, cisandrones, cisandrol, gomisin, and the like, which have been used in oriental medicine, bronchitis, and the like.

The liver, green skin, fat milk, casualty, Schizandra chinensis, which has been used in herbal medicine for a long time, is a plant whose human safety has already been confirmed by ingestion of the body, and is a safe plant having little irritation to skin and mucous membranes. Provided is a composition for preventing and treating athlete's foot eczema containing an extract thereof.

The composition of the present invention is a composition comprising the extract of the plants based on the soap base. If the content of the extract is less than 0.01% by weight, it does not show a significant effect, and when the content is more than 10% by weight, the increase of the antimicrobial effect is low compared to the increase of the economic efficiency.

Since the composition of the present invention contains the liver, green skin, fat milk, casualty, and Schisandra chinensis extract in a conventional base, skin exfoliants such as salicylic acid, alpha hydroxy acid, beta hydroxy acid or sulfur are used to enhance clinical effect. Can be added. In addition, flavors, pigments, fungicides, antioxidants, moisturizers and thickeners for improving physical properties, inorganic salts or synthetic polymer materials may be selected and added.

Compositions such as sagan, cheongpi, fat milk, casualty, Schisandra chinensis extract, soaps, creams, etc. composition was performed clinical trials to evaluate the bactericidal power and the therapeutic effect of athlete's foot eczema against causative bacteria of athlete's foot eczema, and as a result These compositions containing the liver, green skin, fat milk, casualty, and Schisandra chinensis extract did not show side effects such as skin redness, and also showed excellent therapeutic effect of athlete's foot eczema.

The composition of the present invention can be applied to any of the properties, such as liquid, cream, paste, and solid, which are different formulations, and can be easily applied to conventional manufacturing methods.

Hereinafter, the present invention will be described in more detail with reference to Examples and Comparative Examples. However, these Examples are only for illustrating the present invention and the present invention is not limited thereto.

EXAMPLE

Example 1

Preparation of Sandan, Cheongpi, Fat Milk, Casualties, and Schizandra chinensis Extracts

500 g of dried sand, green skin, fat milk, filamentous and schizandra chinensis were ground to a size of 10 to 200 mesh using a herbal medicine grinder.

250 milliliters of an 80% (v / v) ethanol aqueous solution was added to the ground medicinal herbs, and the mixture was cooled at room temperature for 48 hours, and then filtered using a Whatman # 2 filter paper and a vacuum filter paper.

Each filtered extract was concentrated under reduced pressure at 45 ° C. to obtain 100-105 g of extract, respectively.

Evaluation of Antimicrobial Activity against Athlete's Foot Eczema by Sagan, Cheongpi, Fat Milk, Casualties, and Schizandra chinensis Extracts

In order to determine the antimicrobial activity against athlete's foot fungi, paper disk test was carried out for each of the liver, green skin, fat milk, casualty and schizandra extracts prepared above.

5 days in medium of potato dextrose broth containing 1% corn oil to activate test strains trichophyton mentagrophytes and epidermophyton floccosum. Pre-culture.

The culture medium thus prepared was plated on a solid medium (potato dextrose broth + 1.5% agar) on and dried.

40 μl each of the liver, green skin, fat milk, filamentous and Omija extract 10% aqueous solution prepared above was dropped onto 8 mm paper discs, and then placed on the solid medium and incubated in a 30 ° C. incubator for 3 days.

The antimicrobial activity was evaluated by observing the bacterial growth inhibition region generated around the disc and measuring the size of the ring.

Table 1 shows the size of the bacterium growth inhibitory ring in 10% aqueous solution of liver, green skin, fat milk, filamentous and Schizandra chinensis extract.

TABLE 1

division Sagan extract Blue Skin Extract Fat milk extract Casualty extract Schizandra Extract Inhibition of growth and spore formation inhibition of test strain (bacterial growth ring size, mm) Trichophyton Mentagrophyte 23 25 22 31 24 Epidermophyton Floco Island 12 20 37 35 28

Examples 2-5, Comparative Examples 1-2

Manufacture of soap

Soap base (including moisture and various additives) and each of the plant extracts prepared in Example 1 were mixed well in a mixer in the composition ratio of Table 2 below, and then extruded, cut and molded in a soap maker to prepare a solid soap composition.

Each of the extracts could be prepared by including not only general soap, but also synthetic soap and composite soap by a conventional soap manufacturing method.

TABLE 2

Raw material (% by weight) Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Soap base 99.99 99.90 99 95 100 99.995 extract 0.01 0.10 One 5 0 0.005 Spices (extrapolation) Quantity Quantity Quantity Quantity Quantity Quantity Color (extrapolation) Quantity Quantity Quantity Quantity Quantity Quantity Antioxidant (Extrapolated) (EDTA) Quantity Quantity Quantity Quantity Quantity Quantity

Evaluation of antimicrobial activity against athlete's foot eczema

In order to confirm the prophylactic and therapeutic effect of athlete's foot eczema on the soap compositions prepared above, the antibacterial activity test for trichophyton mentagrophyte and epidermophytone flocosum, which are athlete's foot eczema, was carried out as follows.

After adding 5% aqueous solution of the soap composition prepared above to the fungal medium, the activated test strain was inoculated into each medium, and then cultured at 30 ° C. for 3 days, and the growth patterns of athlete's foot eczema in each medium were observed. . Here, each composition was tested with five media and the average value was taken.

Antibacterial activity against athlete's foot eczema was evaluated as the degree of inhibition of the growth of athlete's foot eczema. Here, the growth of the bacteria on the basis of the case where the growth of the fungus eczema causing bacteria is not inhibited as in the medium of the soap composition which does not include the extract as described above, 5 when the bacteria grow well and 0 when the bacteria do not grow at all. According to the relative score of 0, 1, 2, 3, 4, 5, it was evaluated as effective when the average score of five media per composition is 2 or less.

The results are shown in Table 3. The results in Table 3 are representative average values of the liver, green skin, fat milk, casualty, Schizandra extract prepared in Example 1 exhibited the same antimicrobial effect, respectively. In addition, soap compositions prepared using extracts selected by combining two or more of the above extracts showed the same results.

TABLE 3

division Example 2 Example 3 Example 4 Example 5 Comparative Example 1 Comparative Example 2 Growth degree in the medium of the test strain Trichophyton Mentagrophyte 1.8 One 0.2 0 5 4 Epidermophyton Floco Island 1.8 1.2 0.4 0 5 4.2

Examples 6-10, Comparative Example 3

Manufacture of cream

Combination of cream base oil component, aqueous component, surfactant and the like and each extract prepared in Example 1 was emulsified well in a mixer in the composition ratio of Table 4 below, degassed, filtered, cooled to prepare a cream composition. Preservatives, chelating agents, fragrances and pigments were added to the composition as an additive, and the formulation was prepared in oil / water so that a small amount of oily components were present.

In addition, each of the extracts prepared in Example 1 or mixed in a common oil / water cream base may be contained in the composition, and the fragrance, pigments, antioxidants, preservatives, etc. could be added to improve the physical properties. It could also be prepared by the addition of synthetic or natural materials.

TABLE 4

Raw material (% by weight) Example 6 Example 7 Example 8 Example 9 Example 10 Comparative Example 3 Stearic acid 8 8 8 8 8 8 Stearyl alcohol 4 4 4 4 4 4 Butyl stearate 6 6 6 6 6 6 Propylene glycol 5 5 5 5 5 5 Glycerin Monostearate 2 2 2 2 2 2 Potassium hydroxide 0.40 0.40 0.40 0.40 0.40 0.40 extract 0.01 0.10 One 5 10 0 Purified water 74.59 74.50 73.60 69.60 64.60 74.60 Spices (extrapolation) Quantity Quantity Quantity Quantity Quantity Quantity Color (extrapolation) Quantity Quantity Quantity Quantity Quantity Quantity Preservative (extrapolation) Quantity Quantity Quantity Quantity Quantity Quantity

Evaluation of antimicrobial activity against athlete's foot eczema

In order to confirm the prophylactic and therapeutic effects of athlete's foot eczema on the cream compositions prepared above, the antibacterial activity test on trichophyton mentagrophyte and epidermophytone phlocosome, which are athlete's foot eczema, Examples 2 to 5 and Comparative Example 1 , 2 was carried out as in.

The results are shown in Table 5. The results of Table 5 are representative average values of the liver, green skin, fat milk, casualty, Schizandra extract prepared in Example 1 each showed the same antimicrobial effect. In addition, cream compositions prepared using extracts selected by combining two or more of the above extracts showed the same result.

TABLE 5

division Example 6 Example 7 Example 8 Example 9 Example 10 Comparative Example 3 Growth degree in the medium of the test strain Trichophyton Mentagrophyte 1.6 1.2 0.2 0 0 5 Epidermophyton Floco Island 1.8 1.4 0.4 0 0 5

Composition comprising 0.01 ~ 10% by weight of the liver, green skin, fat milk, casualty, Schizandra chinensis extract of the present invention exhibits excellent antimicrobial effect against trichophyton mentagrophyte and epidermophytone phlocosome, the causative eczema causative agents, to prevent athlete's foot eczema and It can be used for treatment.

In particular, the soap composition can be applied to the drug at the same time to wash the infected area, thereby eliminating the hassle of continuous drug application.

Claims (3)

Trichophyton mentagrophytes and epidermophyton floccosum, which are one or more extracts selected from the group consisting of sand extract, green skin extract, fat milk extract, casualty extract, and schizandra extract, which cause athlete's foot eczema Athlete's foot eczema preventive and therapeutic composition exhibiting an antimicrobial effect against). The method of claim 1, The liver, green skin, fat milk, casualty or Schizandra extract is contained in the composition 0.01 to 10% by weight. The method of claim 1, The liver, green skin, fat milk, casualty or Schisandra chinensis extract a) pulverizing the plant medicament selected from the group consisting of dried loin, ground skin, fat milk, casualty and schizandra into powder of 10 to 200 mesh size with a grinder; b) The pulverized drug is purified water, methanol, ethanol, propanol, butanol, glycerol, ethylene glycol, propylene glycol, 1,3-butylene glycol, methyl acetate, ethyl acetate, benzene, hexane, diethyl ether, dichloro Extraction by chilling for 48 hours or more in an extraction solvent selected from the group consisting of methane and acetone; c) filtering the extract; And d) concentrating the filtered extract under reduced pressure Composition prepared by the method comprising a.