KR100512028B1 - Preparation of functional beverage containing chlorophylls and carotine from seaweed - Google Patents

Preparation of functional beverage containing chlorophylls and carotine from seaweed Download PDF

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KR100512028B1
KR100512028B1 KR10-2002-0018571A KR20020018571A KR100512028B1 KR 100512028 B1 KR100512028 B1 KR 100512028B1 KR 20020018571 A KR20020018571 A KR 20020018571A KR 100512028 B1 KR100512028 B1 KR 100512028B1
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algae
pigment
weight
extract
seaweed
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KR20030079506A (en
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김인철
순 택 정
유정욱
강형동
박수미
이현희
이홍열
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학교법인 봉석학원 동아인재대학
순 택 정
김인철
이홍열
정홍술
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/58Colouring agents
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/60Sweeteners
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52Adding ingredients
    • A23L2/68Acidifying substances
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/40Colouring or decolouring of foods
    • A23L5/42Addition of dyes or pigments, e.g. in combination with optical brighteners
    • A23L5/46Addition of dyes or pigments, e.g. in combination with optical brighteners using dyes or pigments of microbial or algal origin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2200/00Function of food ingredients
    • A23V2200/04Colour
    • A23V2200/044Colouring

Abstract

본 발명은 기능성 음료에 관한 것으로, 더욱 상세하게는 설탕, 아스파탐, 프락토올리고당, 구연산, 과일농축액를 함유하는 음료 조성물에 해조류로부터 추출하여 농축된 농축액인 색소 추출물을 더 혼합하여 만들어지며, The present invention relates to a functional beverage, and more particularly, to a beverage composition containing sugar, aspartame, fructooligosaccharide, citric acid, and fruit concentrate is made by further mixing a pigment extract, which is a concentrated concentrate extracted from seaweed,

해조류를 채취하고 채취된 해조류를 맑은 물에 세척한 후 건조하는 건조 과정(S1)과 ; 건조된 해조류를 분쇄하는 분쇄 과정(S2)과 ; 분쇄된 해조류에 아세톤은 가한 후 교반하여 해조류에 함유된 색소를 추출하는 색소 추출 과정(S3)과 ; 색소가 추출된 해조류를 글라스 필터를 이용해 걸러낸 후 감압 농축하여 아세톤을 분리해내는 여과 과정(S4)과 ; 아세톤이 분리된 색소를 농축하는 농축 과정(S5)과 ; 농축된 색소 추출물에 설탕, 아스파탐, 솔비톨, 구연산 및 과일농축액을 혼합하는 배합 과정(S6)으로 만들어지며, 항암작용, 항궤양작용, 항콜레스테롤 작용, 조혈작용, 혈압강하작용, 항박테리아, 항바이러스 효과, 간장과 쓸개 및 간경화 세포 부활작용, 지혈작용, 강심작용, 말초혈관 확장작용, 상처치유 촉진 작용, 항알레르기 작용 등의 다양한 생리작용에 많은 효과가 있다. Collecting the algae, washing the collected algae in clear water and drying the dried process (S1); Grinding step (S2) of grinding the dried algae; Acetone is added to the ground algae, followed by stirring to extract a pigment contained in the algae (S3); Filtration process (S4) to filter out the algae extracted from the pigment using a glass filter and then concentrated under reduced pressure to separate acetone; A concentration step (S5) of concentrating the dye from which acetone has been separated; It is a compounding process (S6) in which sugar, aspartame, sorbitol, citric acid and fruit concentrate are mixed with concentrated pigment extract, and it is anti-cancer, anti-ulcer, anti-cholesterol, hematopoietic, blood pressure lowering, antibacterial, antiviral Effects, hepatic and gallbladder and liver cirrhosis cell reactivation, hemostatic action, cardiac action, peripheral vascular expansion action, wound healing promotion action, anti-allergic action and a variety of effects are many effects.

Description

해조류의 색소 추출물을 함유하는 기능성 건강 음료 및 그 제조방법{Preparation of functional beverage containing chlorophylls and carotine from seaweed}Functional health beverage containing pigment extract of seaweed and preparation method thereof {Preparation of functional beverage containing chlorophylls and carotine from seaweed}

본 발명은 기능성 음료 및 그 제조에 관한 것으로 보다 상세하게는 해양 녹조류의 색소 추출물을 함유한 기능성 음료 및 그의 제조 방법에 관한 것이다. The present invention relates to a functional beverage and its preparation, and more particularly, to a functional beverage containing a pigment extract of marine green algae and a method for producing the same.

더욱 상세하게는 항암작용, 항궤양작용, 항콜레스테롤작용, 조혈작용, 혈압강하작용, 항박테리아, 항바이러스 효과, 간장, 쓸개 및 간경화 세포 부활작용, 지혈작용, 강심작용, 말초혈관 확장작용, 상처 치유 촉진 작용, 항알레르기 작용, 숙취해소, 천식치유 등의 다양한 기능성이 밝혀지면서 각광을 받고 있는 클로로필(엽록소), 특히 해조 녹조류로부터 추출한 클로로필과 베타-카로틴 등을 주성분으로 하는 기능성음료의 제조방법에 관한 것이다. More specifically, anti-cancer, anti-ulcer, anti-cholesterol, hematopoietic, hypotensive, antibacterial, antiviral, hepatic, gallbladder and liver cirrhosis cell activation, hemostasis, cardiac, peripheral vasodilation, wound In the manufacturing method of functional drinks mainly containing chlorophyll (chlorophyll), which has been spotlighted as various functionalities such as healing promoting action, anti-allergic action, hangover relief and asthma healing, especially chlorophyll and beta-carotene extracted from seaweed green algae It is about.

일반적으로 해조류는 파래, 청각 등과 같은 녹색을 띄는 녹조류, 모자반과 다시마와 같이 갈색을 띄는 갈조류, 김과 우뭇가사리 등과 같이 붉은 색을 띄는 홍조류로 대별된다. 그 중에서 녹조류에는 인체의 혈색소 중 헤모글로빈과 구조가 거의 흡사한 엽록소를 많이 함유하고 있다. In general, algae are divided into green algae such as green and blue, green brown algae such as maban and kelp, and red algae such as laver and sea urchin. Among them, green algae contain a large amount of chlorophyll, which is almost similar in structure to hemoglobin in the human hemoglobin.

엽록소 중에서도 클로로필 a와 b를 함유하고 있다. 세계에 약 1만 종이 알려져 있으며, 그 중 약 90 %가 담수산이고 10 %가 해수산이다. 색소체가 다량의 엽록소를 함유하고 있어 녹색을 띈다. 엽록소 a 및 b를 고등식물과 거의 같은 비율로 함유하고 있으며, 그밖에 고등식물과 같은 카로티노이드 색소를 가진다. 녹조류의 엽상체는 매우 투명한 녹색을 지니고 있는데 색소체로는 엽록체, 베타카로틴, 크산토필(xanthophyll)계의 루틴(lutein)이라는 색소를 지니고 있다. Chlorophyll also contains chlorophyll a and b. About 10,000 species are known in the world, of which about 90% are freshwater and 10% are seawater. The plastid contains a large amount of chlorophyll and is green. Chlorophyll a and b are contained in about the same proportion as higher plants, and other carotenoid pigments are the same as higher plants. The fronds of green algae have a very transparent green color. The chromosome has a pigment called chloroplast, beta carotene, and xanthophyll type lutein.

대표적인 녹조류로는 파래를 예로 들 수 있다. 파래는 시각적으로 시원하고, 미각적으로 신선한 느낌을 준다. 파래는 김에 버금갈 만큼 풍부하고 다양한 영양가를 지니고 있다. 3대 영양소 중에서 탄수화물과 단백질이 매우 풍부하여 체구성 물질로서 뿐만 아니라 열량 면에서도 대단히 우수한 식품이다. 파래에는 비타민 A가 다량으로 포함되어 있을 뿐만 아니라 비타민 B1, 리보플라빈, 나이아신도 상당량 들어 있다. 미네랄로는 칼슘과 철분이 많이 들어 있어 건강식품으로서 최상의 위치에 있다고 할 수 있다. Representative green algae may be scallop. The green is visually cool and tastes fresh. Seaweed is as rich as it is and rich in nutritional value. Carbohydrates and protein among the three major nutrients is very rich in food as well as body composition and calories. The greens not only contain large amounts of vitamin A, but also contain significant amounts of vitamin B1, riboflavin and niacin. Minerals contain a lot of calcium and iron and can be said to be in the best position as a health food.

또한, 파래에는 대장의 연동운동을 돕는 식물성 섬유질이 풍부하게 함유되어 있어 배변을 원활하게 하는 효과가 있다. 오늘날의 음식문화는 양질의 섬유소를 충분하게 함유하지 않은 기름진 단백질류나 인스턴트 식품을 상식하는 경향이 있다. 파래같은 식이섬유를 섭식하는 것은 건강 유지에 큰 도움이 된다. 파래와 영양적으로 유사한 녹로류로 청각이 있다.In addition, the greens are rich in vegetable fiber to help peristalsis of the large intestine has the effect of smoothing bowel movements. Today's food culture tends to be common for oily proteins and instant foods that do not contain enough fiber. Eating dietary fiber, such as greens, can help you stay healthy. This is a nutrient that is nutritionally similar to green onions.

엽록소는 식물이 광합성작용에 의해 스스로 영양분을 생산하는 녹색공장이다. 천연의 유기 반도체로서 태양전지의 역할을 담당해 지구상의 자연 태양광을 지표의 화학에너지로 전환하는 작용을 계속하며, 생식물의 녹색 색소 성분으로 태양 에너지 전도, 유기체의 합성에 관여한다. 식물에 있어서 생명을 만드는 아주 중요한 성분인 것이다. 이것의 특징은 20만배 전자 현미경으로 겨우 존재 확인되는 초미립자이고 머리 부분인 포피린(porphyrin)은 친수성으로 피롤(pyrrole)과 착염(chelate form)으로 연결된 Mg 원자가 존재하며 클로로필린(chlorophyllin)을 구성한다. 꼬리 부분인 피톨(pythol)은 친유성으로 인해 체세포의 단백질 막이 쉽게 부착하며, 복합상태로 존재한다. 또한 촉매효과를 내며 베타-카로틴이 이들 배열이 움직이지 않도록 지지하고 있고 또한 이는 옆에 있는 다른 엽록소도 동시에 지지하는 합리적인 배열을 하고 있다. Chlorophyll is a green plant in which plants produce their own nutrients through photosynthesis. As a natural organic semiconductor, it plays the role of a solar cell and continues to convert natural solar light into earth's chemical energy. It is involved in conducting solar energy and synthesizing organisms as a green pigment component of plants. It is a very important ingredient that makes life in plants. It is characterized by ultrafine particles barely identified by a 200,000-fold electron microscope, and porphyrin, the head part, is hydrophilic and contains Mg atoms linked by pyrrole and chelate form, and constitutes chlorophyllin. Pythol, the tail, is easily attached to the protein membrane of somatic cells due to lipophilic properties, and exists in a complex state. It also catalyzes beta-carotene to keep these arrangements from moving, and it also has a reasonable arrangement to support the other chlorophyll next to it.

이와 같은 식물의 생명을 대표하는 엽록소는 동물의 생명을 대표하는 혈액의 구성성분인 혈색소는 화학구조식이 아주 비슷하여, 포피린(phorphyrin) 구조를 가지고 있는데 단지 다른 것은 엽록소는 가운데 핵 금속원소로 마그네슘(Mg)을 혈색소는 철(Fe)을 함유하고 있는 점이다. 이런 엽록소가 인체에 미치는 효능을 살펴보면 항암작용, 항궤양작용, 항콜레스테롤 작용, 조혈작용, 혈압강하작용, 항박테리아, 항바이러스 효과, 간장과 쓸개 및 간경화 세포 부활작용, 지혈작용, 강심 말초혈관 확장작용, 상처치유 촉진 작용, 항알레르기 작용 등의 특별한 생리작용, 소염작용, 기의 활성화, 간염에 의한 황달 증상, 신장염에 의한 뇨단백, 신경계의 장해에 의한 손, 다리 저림, 정신 안정, 뇌파의 정상화 당뇨병에 의한 눈의 이상, 음주에 의한 악쥐, 성적 쇠퇴의 회복, 숙취해소, 천식치유 등의 효능이 있다. Chlorophyll, which represents the life of such plants, has a chemical structure of hemoglobin, a component of blood that represents the life of animals, and has a phorphyrin structure. Mg) hemoglobin contains iron (Fe). The effects of chlorophyll on the human body include anti-cancer, anti-ulcer, anti-cholesterol, hematopoietic, hypotensive, antibacterial, antiviral effects, hepatic and gallbladder and hepatic cirrhosis, hemostasis, and cardiac peripheral blood vessel expansion. Special physiological actions such as action, wound healing promoting action, anti-allergic action, anti-inflammatory action, activation of the group, symptoms of jaundice caused by hepatitis, urinary protein caused by nephritis, hand, leg numbness due to nervous system disorders, normalization of brain waves Eye problems caused by diabetes, drinking rats, recovery from sexual decline, hangover relief, asthma healing, etc. are effective.

본 발명은 상기한 바와 같은 해양 녹조류에 함유되어 있는 유용물질 중 클로로필과 베타-카로틴 등 색소를 유기 용매를 사용하여 추출한 후 이를 이용하여 음료를 제조함으로서 전기와 같은 효능을 갖는 기능성 음료를 제공함을 목적으로 한다. An object of the present invention is to provide a functional beverage having the same efficacy as the above by extracting a pigment such as chlorophyll and beta-carotene among the useful substances contained in the marine green alga as described above using an organic solvent and preparing a beverage using the same. It is done.

상기와 같은 목적을 갖는 본 발명은 통상의 음료에 있어서, 상기 음료의 음료 조성물에 해조류로부터 추출된 색조 추출물을 더 혼합하여 이루어짐을 특징으로 하는 해조류의 색소 추출물을 함유하는 기능성 건강 음료에 의해 이루어진다. The present invention having the above object is made by a functional health beverage containing a pigment extract of seaweeds, characterized in that it is made by further mixing the color tone extract extracted from seaweeds in the beverage composition of the beverage.

또한 본 발명의 목적은 해조류를 채취하고 채취된 해조류를 맑은 물에 세척한 후 건조하는 건조 과정(S1)과 ; 건조된 해조류를 분쇄하는 분쇄 과정(S2)과 ; 분쇄된 해조류에 아세톤은 가한 후 교반하여 해조류에 함유된 색소를 추출하는 색소 추출 과정(S3)과 ; 색소가 추출된 해조류를 글라스 필터를 이용해 걸러낸 후 감압 농축하여 아세톤을 분리해내는 여과 과정(S4)과 ; 아세톤이 분리된 색소를 농축하는 농축 과정(S5)과 ; 농축된 색소 추출물에 설탕, 아스파탐, 솔비톨, 구연산 및 과일농축액을 혼합하는 배합 과정(S6)으로 이루어짐을 특징으로 하는 해조류의 색소 추출물을 함유하는 기능성 건강음료의 제조 방법에 의해 이루어진다.In addition, an object of the present invention is the drying step of collecting the seaweed and washing the collected seaweed in clear water and then drying (S1); Grinding step (S2) of grinding the dried algae; Acetone is added to the ground algae, followed by stirring to extract a pigment contained in the algae (S3); Filtration process (S4) to filter out the algae extracted from the pigment using a glass filter and then concentrated under reduced pressure to separate acetone; A concentration step (S5) of concentrating the dye from which acetone has been separated; It is made by a method for producing a functional health beverage containing a pigment extract of seaweed, characterized in that the mixture consisting of a mixture (S6) of sugar, aspartame, sorbitol, citric acid and fruit concentrate in the concentrated pigment extract.

더욱이 본 발명의 목적은 상기 음료 조성물은 설탕 0.5∼5.0중량부, 아스파탐 0.01∼0.05중량부, 프락토올리고당 1∼10중량부, 구연산 0.05∼0.5중량부, 과일농축액 0.1∼1중량부로 하고, 상기 해조류의 색소 추출물은 해조류로부터 추출하여 농축된 농축액으로 0.1∼1.0중량부를 함유하게 하며, 상기 해조류는 녹조류로서 파래, 청각 중의 어느 하나를 사용함에 의해 이루어질 수 있다.Furthermore, an object of the present invention is the beverage composition is 0.5 to 5.0 parts by weight of sugar, 0.01 to 0.05 parts by weight of aspartame, 1 to 10 parts by weight of fructooligosaccharide, 0.05 to 0.5 parts by weight of citric acid, 0.1 to 1 parts by weight of fruit concentrate, The pigment extract of seaweed is contained from 0.1 to 1.0 parts by weight as a concentrated extract extracted from the seaweed, the seaweed can be made by using any one of green, auditory as green algae.

이하 본 발명에 따른 해조류의 색소 추출물을 함유하는 기능성 건강 음료 및 그 제조방법의 일예를 상세하게 설명한다. 그러나, 본 발명이 이들 실시 예에 국한되는 것은 아니다.Hereinafter will be described in detail an example of a functional health drink containing a pigment extract of seaweed according to the present invention and a method for producing the same. However, the present invention is not limited to these examples.

도 1은 해조류로부터 클로로필과 베타-카로틴의 추출 및 농축 이를 이용한 기능성 음료의 제조 공정을 도시한 공정도이고, 도 2는 해양 녹조류로부터 추출한 색소의 박층크로마토그래피이다.1 is a flow chart illustrating a process for preparing a functional beverage using the extraction and concentration of chlorophyll and beta-carotene from seaweed, Figure 2 is a thin layer chromatography of the pigment extracted from marine algae.

본 발명에 따른 해조류의 색소 추출물을 함유하는 기능성 건강 음료는 해조류의 색조 추출물을 함유하고 있다. The functional health drink containing the pigment extract of the seaweed according to the present invention contains the color extract of the seaweed.

상기 해조류로는 녹조류가 바람직하며, 특히 파래와 청각 등이 사용되며, 이들로부터 색조 추출물을 추출해 낸다. As the algae, green algae are preferable, and green seaweed and hearing are used, and a color tone extract is extracted from them.

색조 추출물을 추출하는 방법은 다음과 같다. How to extract the color tone extract is as follows.

해양 녹조류인 파래와 청각을 채취 또는 시중에서 구입하여 세척하고, 약 55℃내지 65℃의 온도에서 건조하는 건조 과정(S1)을 통하여 건조하고, 건조된 해조류를 분쇄 과정(S2)에서 약 45내지 55메쉬로 분쇄하며, 분쇄된 해조류와 아세톤을 1 : 20중량비로 혼합하고 약 15내지 25℃의 온도에서 약 24시간 교반하여 색소를 추출하하는 색소 추출 과정(S3)을 통하여 색소를 추출한다. Sea green and algae, marine green algae are collected or purchased on the market, washed, dried through a drying process (S1) which is dried at a temperature of about 55 ° C to 65 ° C, and dried seaweeds are about 45 to about 45 in a grinding process (S2). The mixture is pulverized to 55 mesh, and the pulverized seaweed and acetone are mixed in a 1:20 weight ratio, and the pigment is extracted through a pigment extraction process (S3) for extracting a pigment by stirring at a temperature of about 15 to 25 ° C. for about 24 hours.

상기 색소 추출 과정(S3)에서 추출된 색소 추출물은 농도가 낮음으로 이를 발효 주정을 이용하여 10내지 15배로 농축하는 농축 과정(S5)을 통하여 농축하여 사용한다. The pigment extract extracted in the pigment extraction process (S3) is used to concentrate through a concentration process (S5) to concentrate it 10 to 15 times using a fermentation alcohol as the concentration is low.

상기와 같이 농축된 색소 추출물에 음료 조성물을 혼합하여 기능성 음료가 만들어진다. Functional drinks are made by mixing the beverage composition with the concentrated pigment extract as described above.

상기의 과정에서 색소 추출물을 추출하는 원재료인 해조류의 종류에 따른 추출 색소의 박층크로마토그래피 결과는 도 2와 같으며, 색소 함량은 표 1과 같다.In the above process, the thin layer chromatography results of the extracted pigments according to the type of seaweed, which is a raw material for extracting the pigment extract, are shown in FIG. 2, and the pigment content is shown in Table 1 below.

상기와 같이 추출된 해양 녹조류의 색소를 이용한 기능성 음료는 해양 녹조류인 파래와 청각으로부터 추출한 색소 추출물에 감미료로 설탕, 아스파탐, 이소말토올리고당, 프락토올리고당, 솔비톨을 첨가하였고, 산미료는 구연산을 사용하였다. 농축과즙으로는 사과농축액과 또는 유자농축액을 사용하였고, 기타 첨가물로는 베타-사이클로덱스트린을 사용하였다. 상기에서 사과나 유자 농축액을 대신하여 다른 과일의 농축액을 사용할 수 있음은 본 발명이 속하는 분야에 종사하는 당업자에게는 쉽게 이해될 수 있는 것으로 이러한 일예가 본 발명의 청구범위를 제한하는 것은 아니다.The functional beverage using the pigments of marine green algae extracted as described above was added sugar, aspartame, isomaltoligosaccharides, fructooligosaccharides, and sorbitol as sweeteners to the pigment extracts extracted from marine green algae, green and green. . Apple concentrate and citron concentrate were used as concentrated juices, and beta-cyclodextrin was used as other additives. The use of concentrates of other fruits in place of apples or citron concentrate may be readily understood by those skilled in the art to which the present invention pertains, and one example thereof does not limit the scope of the present invention.

관능검사의 평가는 7점 평가를 하였으며, 평가된 결과는 ANOVA에 의해 분석하였고 유의성 검정은 Duncan's Multiple Range Test를 사용하였다.The sensory evaluation was evaluated by 7 points. The results were analyzed by ANOVA, and the significance test was performed by Duncan's Multiple Range Test.

당의 조합에 따른 기호도 조사Investigation of preference according to sugar combination

파래와 청각의 색소 추출물을 이용한 기능성 음료 제조를 위한 당의 최적 조합비를 결정하기 위한 조합비는 표 3과 같으며, 이의 관능평가결과는 표 4와 같다. Combination ratios for determining the optimal combination ratio of sugars for the production of functional beverages using the extract of green and blue pigments are shown in Table 3, the sensory evaluation results are shown in Table 4.

추출되어진 색소의 희석농도에 따른 기호도 조사Investigation of preference according to dilution concentration of extracted pigment

음료를 제조하기 전에 추출되어진 색소를 어느 정도 희석하였을 때 기호도가 가장 좋은지 알아보기 위하여 음료수 제조에 사용되는 물에 추출액을 각각 0.05, 0.10, 0.25, 0.50, 1.00% 씩 첨가하여 관능평가를 실시한 결과 파래, 청각 모두 0.25% 농도가 가장 좋은 기호도를 보였다. 그래서 음료수를 제조할 때 추출액을 0.25% 첨가하기로 결정하였다. In order to find out the best preference when diluting the extracted pigment prior to preparing the beverage, sensory evaluation was carried out by adding 0.05, 0.10, 0.25, 0.50, and 1.00% of the extract to the water used to prepare the beverage, respectively. For both hearing and hearing, 0.25% concentration showed the best preference. So it was decided to add 0.25% of the extract when preparing the beverage.

해양 녹조류인 파래와 청각의 색소추출물을 이용한 기능성 건강음료에 첨가되는 음료 조성물의 배합비는 색소 추출물 0.1∼1.0중량부에 설탕 0.5∼5.0중량부, 아스파탐 0.01∼0.05중량부, 프락토올리고당 1∼10중량부, 구연산 0.05∼0.5중량부, 사과농축액 0.1∼1중량부를 첨가함이 바람직하나 다음의 관능 평가 결과를 감안할 때 최종 제조 조건을 결정하기 위한 배합비는 표과 같으며, 관능평가 결과는 표 8에 나타냈다. The blending ratio of the beverage composition added to the functional health beverage using green and green pigment extracts of marine green algae is 0.1-1.0 parts by weight of pigment extract, 0.5-5.0 parts by weight of sugar, 0.01-0.05 parts by weight of aspartame, and 1-10 of fructooligosaccharide. It is preferred to add parts by weight, 0.05 to 0.5 parts by weight of citric acid, and 0.1 to 1 parts by weight of apple concentrate, but considering the following sensory evaluation results, the blending ratio for determining the final manufacturing conditions is shown in the table. Indicated.

관능 평가 결과 파래에서 추출한 색소추출물을 이용한 음료의 경우 그 배합비가 색소 추출물 0.25 중량부, 설탕 1.0 중량부, 아스파탐 0.2 중량부, 솔비톨 5.0 중량부, 구연산 0.1 그리고 사과농축액 0.4 중량부로 하여 제조한 음료가 관능적으로 가장 우수하였다. As a result of sensory evaluation, the beverage prepared with the extract extracted from the green tea was prepared with a blending ratio of 0.25 parts by weight of pigment extract, 1.0 parts by weight of sugar, 0.2 parts by weight of aspartame, 5.0 parts by weight of sorbitol, 0.1 parts of citric acid and 0.4 parts by weight of apple concentrate. It was the best sensory.

또한, 청각으로부터 추출된 색소 추출물을 이용한 경우는 설탕을 첨가하지 않고 색소 추출물 0.25 중량부, 아스파탐 0.2 중량부, 솔비톨 5.0 중량부, 구연산 0.1 중량부 그리고 사과농축액 0.4 중량부로 혼합하여 제조한 음료가 관능적으로 가장 우수하였다.In addition, in the case of using the pigment extract extracted from the hearing, the beverage prepared by mixing the sugar extract 0.25 parts by weight, aspartame 0.2 parts by weight, sorbitol 5.0 parts by weight, citric acid 0.1 parts by weight and apple concentrate 0.4 parts by weight Was the best.

상기와 같이 본 발명에 따른 해조류 특히, 녹조류인 파래 또는 청각의 색조 추출물을 이용한 건강 음료는 해조류의 색소 추출물의 다양한 기능성에 의한 국민건강 증진 효과, 특히 성인병 예방에 기여할 수 있으며 저가의 중국산에 의해 수출에 어려움을 겪고 있는 국내산 해조류 소비촉진 및 지속적인 생산기반 확보, 그리고 어민의 소득증대에 기여할 수 있을 것이다. As described above, the seaweeds according to the present invention, in particular, the health drink using the green or green algae extract extract of the green algae can contribute to the improvement of national health by the various functionalities of the seaweed's pigment extract, in particular the prevention of adult diseases and export by low-cost Chinese It will contribute to the promotion of consumption of domestic algae, which is struggling, and to the continuous production base and increase of income of fishermen.

도 1은 해양 녹조류로부터 클로로필과 베타-카로틴의 추출 및 농축 이를 이용한 기능성 음료의 제조 공정을 도시한 공정도이고, 1 is a flowchart illustrating a process for preparing a functional beverage using the extraction and concentration of chlorophyll and beta-carotene from marine algae,

도 2는 해양 녹조류로부터 추출한 색소의 박층크로마토그래피2 is a thin layer chromatography of pigments extracted from marine green algae.

Claims (6)

삭제delete 음료 조성물에 해조류로부터 추출된 색조 추출물을 더 혼합하여 이루어진 통상의 음료에 있어서, 상기 음료 조성물은 아스파탐 0.01∼0.05중량부, 프락토올리고당 1∼10중량부, 구연산 0.05∼0.5중량부, 과일농축액 0.1∼1중량부이고, 상기 해조류의 색소 추출물은 해조류로부터 추출하여 농축된 농축액으로 0.1∼1.0중량부를 함유하며, 설탕 0.5∼5.0중량부를 선택적으로 혼합하여 이루어짐을 특징으로 하는 해조류의 색소 추출물을 함유하는 기능성 음료.In a conventional beverage made by further mixing the color tone extract extracted from seaweed in the beverage composition, the beverage composition is 0.01 to 0.05 parts by weight of aspartame, 1 to 10 parts by weight of fructooligosaccharide, 0.05 to 0.5 parts by weight of citric acid, fruit concentrate 0.1 It is-1 part by weight, the pigment extract of the seaweed contains 0.1-1.0 parts by weight of the concentrated extract extracted from the seaweed, containing 0.5-5.0 parts by weight of sugar, containing the pigment extract of seaweeds Functional drink. 해조류를 채취하고 채취된 해조류를 맑은 물에 세척한 후 건조하는 건조 과정(S1)과 Drying process (S1) and collecting the algae and washing the collected algae in clear water and drying 건조된 해조류를 분쇄하는 분쇄 과정(S2)과 ; Grinding step (S2) of grinding the dried algae; 분쇄된 해조류에 소정량의 아세톤을 가한 후 교반하여 해조류에 함유된 색소를 추출하는 색소 추출 과정(S3)과 ; A dye extraction step (S3) of adding a predetermined amount of acetone to the ground algae, followed by stirring to extract the pigment contained in the algae; 색소가 추출된 해조류를 글라스 필터를 이용해 걸러낸 후 감압 농축하여 아세톤을 분리해내는 여과 과정(S4)과 ; Filtration process (S4) to filter out the algae extracted from the pigment using a glass filter and then concentrated under reduced pressure to separate acetone; 아세톤이 분리된 색소를 농축하는 농축 과정(S5)과 ; A concentration step (S5) of concentrating the dye from which acetone has been separated; 농축된 색소 추출물에 설탕, 아스파탐, 솔비톨, 구연산 및 과일농축액을 혼합하는 배합 과정(S6)으로 이루어짐을 특징으로 하는 해조류의 색소 추출물을 함유하는 기능성 음료의 제조 방법.A method for producing a functional beverage containing a pigment extract of seaweed, characterized by consisting of a blending process (S6) of mixing the concentrated pigment extract with sugar, aspartame, sorbitol, citric acid and fruit concentrate. 제3항에 있어서, The method of claim 3, 상기 건조 과정(S1)은 약 55℃내지 65℃의 온도에서 이루어지고, 상기 분쇄 과정(S2)은 건조된 해조류를 약 45내지 55메쉬로 분쇄함에 의하여 이루어지며, 상기 색소 추출 과정(S3)은 분쇄된 해조류와 아세톤의 비율이 1 : 20중량비로 혼합되고 약 15내지 25℃의 온도에서 교반하여 색소를 추출하여 이루어지고, 상기 농축 과정(S5)은 발효 주정을 이용하여 10내지 15배로 농축하여 이루어지며, 상기 배합 과정(S6)은 색소 농축액 0.1∼1.0중량부에 설탕 0.5∼5.0중량부, 아스파탐 0.01∼0.05중량부, 프락토올리고당 1∼10중량부, 구연산 0.05∼0.5중량부, 사과농축액 0.1∼1중량부를 첨가하여 이루어짐을 특징으로 하는 해조류의 색소 추출물을 함유하는 기능성 음료의 제조 방법.The drying process (S1) is made at a temperature of about 55 ℃ to 65 ℃, the grinding process (S2) is made by grinding the dried seaweed to about 45 to 55 mesh, the pigment extraction process (S3) is The ratio of pulverized seaweed and acetone is mixed at a weight ratio of 1:20 and stirred at a temperature of about 15 to 25 ° C. to extract pigments. The concentration process (S5) is concentrated to 10 to 15 times using fermentation alcohol. Wherein, the compounding process (S6) is 0.5 to 5.0 parts by weight of sugar, 0.1 to 1.0 parts by weight of pigment concentrate, 0.01 to 0.05 parts by weight of aspartame, 1 to 10 parts by weight of fructooligosaccharide, 0.05 to 0.5 parts by weight of citric acid, apple concentrate A method for producing a functional beverage containing a pigment extract of seaweed, comprising 0.1 to 1 part by weight. 제2항 내지 제4항 중 어느 한 항에 있어서, The method according to any one of claims 2 to 4, 상기 해조류는 녹조류로서 파래, 청각 중의 어느 하나임을 특징으로 하는 해조류의 색소 추출물을 함유하는 기능성 음료.The seaweed is a functional beverage containing a pigment extract of the seaweed, characterized in that the green algae of any one of the green, blue. 제2항 내지 제4항중 어느 한 항에 있어서, The method according to any one of claims 2 to 4, 상기 색조추출물은 클로로필과 베타카로틴임을 특징으로하는 해조류의 색소추출물을 함유하는 기능성음료.The color extract is a functional drink containing a pigment extract of seaweed, characterized in that chlorophyll and beta carotene.
KR10-2002-0018571A 2002-04-04 2002-04-04 Preparation of functional beverage containing chlorophylls and carotine from seaweed KR100512028B1 (en)

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KR940003495A (en) * 1992-08-19 1994-03-12 박정윤 Manufacturing method of beverage using seaweed
KR950030875A (en) * 1994-05-30 1995-12-18 전종한 Extraction method of natural antioxidants
JPH083468A (en) * 1994-06-21 1996-01-09 New Nitsupo:Kk Production of colorant, production of colored seaweed extract, and production of seaweed food material
JPH11169137A (en) * 1997-12-17 1999-06-29 Riken Shokuhin Kk Low-salt/low-odor sea alga, its production and food/drink using the same
KR20020031289A (en) * 2000-10-23 2002-05-01 윤천기 the processing method of chlorophyll drink

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR940003495A (en) * 1992-08-19 1994-03-12 박정윤 Manufacturing method of beverage using seaweed
KR950030875A (en) * 1994-05-30 1995-12-18 전종한 Extraction method of natural antioxidants
JPH083468A (en) * 1994-06-21 1996-01-09 New Nitsupo:Kk Production of colorant, production of colored seaweed extract, and production of seaweed food material
JPH11169137A (en) * 1997-12-17 1999-06-29 Riken Shokuhin Kk Low-salt/low-odor sea alga, its production and food/drink using the same
KR20020031289A (en) * 2000-10-23 2002-05-01 윤천기 the processing method of chlorophyll drink

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