KR100462953B1 - The microbe fertilizer's producting method be used by microbe and humic acid - Google Patents

The microbe fertilizer's producting method be used by microbe and humic acid Download PDF

Info

Publication number
KR100462953B1
KR100462953B1 KR10-2002-0024054A KR20020024054A KR100462953B1 KR 100462953 B1 KR100462953 B1 KR 100462953B1 KR 20020024054 A KR20020024054 A KR 20020024054A KR 100462953 B1 KR100462953 B1 KR 100462953B1
Authority
KR
South Korea
Prior art keywords
humic acid
powder
soil
acid
microorganisms
Prior art date
Application number
KR10-2002-0024054A
Other languages
Korean (ko)
Other versions
KR20020036830A (en
Inventor
육영민
김현수
유대식
이인선
Original Assignee
학교법인 계명기독학원
김현수
육영민
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 학교법인 계명기독학원, 김현수, 육영민 filed Critical 학교법인 계명기독학원
Priority to KR10-2002-0024054A priority Critical patent/KR100462953B1/en
Publication of KR20020036830A publication Critical patent/KR20020036830A/en
Application granted granted Critical
Publication of KR100462953B1 publication Critical patent/KR100462953B1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00—Fertilisers characterised by their form
    • C05G5/20—Liquid fertilisers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Fertilizers (AREA)

Abstract

본 발명은 미생물과 부식산을 이용한 미생물 비료의 제조방법에 관한 것이고 토양의 개량과 식물의 증산 및 성장식물이 병해에서의 면역력을 높일 수 있도록 함에 목적을 둔 것이며 항진균성물질을 생산하는 미생물을 배양 정균시킨 배양액 또는 배양분말과 부식물질에서 추출한 부식산액 또는 분말 부식산을 혼합하여 제조하는 방법이다.The present invention relates to a method for producing microbial fertilizers using microorganisms and humic acid, and aims at improving soil and increasing plant immunity and growing plant immunity, and cultivating microorganisms producing antifungal substances. It is a method of preparing a mixture of the cultured culture medium or culture powder and the humic acid or powder humic acid extracted from the caustic.

이와 같은 본 발명의 비료를 토양 또는 식물에 엽면살포를 하게 되면 미생물이 생산하는 항진균성 물질은 식물의 성장과정에서 곰팡이 병해 발생시 곰팡이균의 생육을 억제 또는 사멸하게 되고, 부식산은 유기탄소원으로서 생장식물의 영양원으로 사용되어져 뿌리의 발육과 생육에 도움을 주고 자가 면역력을 높여주게 되며 토양속에 잔류하는 비료염과 흡착하여 연작장해를 완화시켜주고, 토양과의 혼합으로 알칼리화 되면서 완충작용을 하게 되는 것이다.When foliar spraying of the fertilizer of the present invention on the soil or plants, the antifungal substance produced by the microorganisms inhibits or kills the growth of the fungus when a fungal disease occurs during the growth of the plant, and the humic acid is a growth plant as an organic carbon source. It is used as a nutritional source of nutrients to help root development and growth and to increase autoimmunity, and to adsorb with fertilizer salts remaining in the soil to alleviate serial disturbances, and to be buffered by alkalizing by mixing with soil.

Description

미생물과 부식산을 이용한 미생물 비료의 제조방법{ The microbe fertilizer's producting method be used by microbe and humic acid }The microbe fertilizer's producting method be used by microbe and humic acid}

본 발명은 미생물과 부식산(Humic Acid)을 이용한 미생물 비료의 제조방법에 관한 것이며 부식원료(부엽토,초탄, 이탄등)에서 부식산을 추출하고 여기에 미생물을 배지에 접종 배양시켜서 정균시킨 미생물을 액상 또는 분말의 부식산과 혼합가공하여 무독성 생물비료를 제조하여 토양을 개량 연작장애를 해소하고, 재배식물의 생육을 촉진시키고 면역력을 키우고 재배식물의 품질을 높일 수 있도록 한 것이다.The present invention relates to a method for producing a microbial fertilizer using microorganisms and humic acid (Humic Acid) and extracts the humic acid from the corrosive raw materials (leafy, peat, peat, etc.) and inoculated and incubated in the medium to the microorganisms By mixing with liquid or powder humic acid to produce non-toxic biofertilizer to improve the soil, to solve the hindrance, improve the growth of cultivated plants, increase immunity and improve the quality of cultivated plants.

오늘날 농작물은 비닐하우스등 시설에 의한 촉성재배와 지역의 특화농업이 날로 증가되고 있다.Today, crops are being increased by faculty cultivation by facilities such as plastic houses and local specialty farming.

이와 같은 지역의 특화농업과 촉성재배는 연작재배로 이어져 연작으로 인한 토양의 염류집적으로 연작에 많은 문제가 발생되고 있다.Specialized farming and promoting cultivation in such regions lead to serial cultivation, which causes a lot of problems in serialization due to salt accumulation of the soil.

즉 동일 농작물의 반복재배로 인하여 토양에 염류집적이 발생되고 염류집적을 해소하기 위하여 옮겨가면서 재배하는 윤작을 하게 되나 농경지가 제한된 지역에는 윤작이 불가능하여 연작시 염류를 해소하는 수단으로 유기물(퇴비, 유기질 비료등)을 다량시비하여 염류의 집적을 줄이고 있으나 노동력의 부족으로 퇴비생산에한계성이 있고 유기질비료등의 경우에도 반복되는 연작에서의 염류해소에는 한계성을 가지게 되는 실정이다.In other words, due to the repeated cultivation of the same crops, salt accumulation occurs in the soil and rotation is carried out to remove salt accumulation. However, crop rotation is impossible in areas with limited cropland, and organic matter (compost, organic matter) is used as a means of eliminating salt during farming. Fertilizer, etc.) is used to reduce the accumulation of salts, but there is a limit to compost production due to lack of labor, and organic fertilizers, etc., have limitations in eliminating salt in repetitive series.

또한 윤작하더라도 농산물의 생산성을 높이기 위하여 화학비료의 과다사용으로 토양이 산성화 및 부영양화가 심해지고 이로 인한 작물의 생육이 원할하지 못하고 면역력이 떨어져 병해에 약하게 되고 농약의 과량살포로 인하여 생산된 농산물에는 농약이 잔류하면서 국민건강을 저해하게 되고 또 수확량이 절감되는 문제점으로 이어지고 있다.In addition, even if it is rotated, the soil is acidified and eutrophicated due to excessive use of chemical fertilizers to increase the productivity of agricultural products, resulting in undesired growth of crops, weakened immunity due to the lack of immunity, and pesticides in agricultural products produced by excessive spraying of pesticides. As this remains, it impedes public health and reduces yields.

이를 해소하는 수단으로 토양에 유기물을 다량 시비하여 염류집적과 토양의 산성화를 줄이거나 또는 윤작을 하게 되나 이는 임대농이 많고 또 농경지가 좁은 지역에서 특화농업으로 발달된 현실에서는 널리 이용되지 못하고 있다.As a means to solve this problem, large quantities of organic matter are applied to the soil to reduce salt accumulation and acidification of the soil, or to rotate it. However, this is not widely used in the realization of specialized agriculture in areas with a large number of rental farms and narrow agricultural land.

또 천연광석분말과 식물즙 및 뮤코라코서스등 각종 미생물을 혼합 제조되는 분말을 토양 또는 재배식물에 투여하는 비료가 개발된 바 있고 이는 작물의 수확을 늘이고 토양의 개량효과를 가져올 수 있도록 하고 있으나 관수시설에 사용하게 될 경우 용수에 용해되지 않고 비중이 커서 관수로의 노즐을 막거나 또는 관내에 잔류되어 분말 상태로 토양시비 또는 엽면시비로 하게 되는 사용상의 결점이 있고 또 광석분말의 경우에는 광석에서 함유하는 각종무기물과 다기공성, 원적외선등 물리적 기능으로 증수효과를 가지게 되는 것이고 또 흙에서 산도(pH)를 변화시키고 토양개량의 기능이 있다고는 하나 광석분말의 무기물이 토양에 분해되어 토양이 개량되고 흙의 산도변화에 이르는 양이 되기에는 많은양의 광석분말이 공급되어야 하므로 경제적이지 못한 결점을 안고 있는 것이다.In addition, fertilizers have been developed in which powders mixed with various microorganisms such as natural ore powders, plant juices and muco racosus are administered to soil or cultivated plants, which increase crop yield and bring about improvement of soil. When used in watering facilities, it does not dissolve in water and has a high specific gravity, so it blocks the nozzle in the waterway or remains in the pipe, so it is ground fertilization or foliar fertilization in powder form, and in the case of ore powder It has the effect of increasing water by physical functions such as various inorganic matters contained in, porosity, and far infrared rays. It also changes acidity (pH) in soil and improves soil, but minerals of ore powder are decomposed into soil and soil is improved. It is economical because a large amount of ore powder must be supplied to reach the change of acidity of soil. You have a fault.

이에 본 발명은 종래의 제반결점을 해소하고 부엽토, 부식토,초탄, 이탄등을 이용하여 추출한 부식산에 배양된 미생물 균주를 혼합하여 토양과 재배식물에 시비하여 농산물의 증수효과와 식물의 면역력을 증강시키고 토양의 개량효과를 가져올 수 있는 길항미생물과 부식산을 이용한 미생물비료의 제조방법을 제공함에 목적이 있다.Accordingly, the present invention solves the conventional defects and mixes the microbial strains cultured in humic acid extracted by using the foliar soil, humus soil, peat, peat, etc., and fertilize the soil and cultivated plants to enhance the effect of increasing the produce and plant immunity. The purpose of the present invention is to provide a method for producing microbial fertilizers using antagonistic microorganisms and humic acid, which can bring about an effect of improving soil.

항진균성 물질을 생산하는 미생물 균주를 배지에서 배양하고 이를 정균시켜 배양액 또는 배양분말을 조성하고 부식물질에서 추출한 부식산액 또는 분말 부식산을 조성하여 미생물과 부식산을 혼합하여 제조하는 것이다.A microbial strain producing an antifungal substance is cultured in a medium and bacteriostatic to prepare a culture medium or a culture powder, and to prepare a humic acid powder or a humic acid extracted from a corrosive substance to prepare a microorganism and a humic acid.

이와 같은 본 발명의 제조공정을 공정별로 상세히 설명하면 다음과 같다.The manufacturing process of the present invention will be described in detail for each process as follows.

[제1공정](미생물의 배양)[Step 1] (cultivation of microorganisms)

항진균성 물질을 생산하는 미생물 균주인 셀로모나스(Cellulomonas), 바실러스 (Bacillus), 스트립토마이세스(Streptomyces), 트레코델마(Trecodelma), 슈도모나스(Pseudomonas)등의 균주를 한종 또는 복수종을 디프코(diffco)사의 LB broth, Nutriunt broth에서 선택한 PDB 또는 머크(Merk)사의 PDA 배지 및 한국과학기술원미생물유전자은행(KCTC)에서 공시된 세균 및 곰팡이 생육배지에 접종시켜 25~40℃에서 2~3일간 미생물을 배양시킨다.Diffco strains of one or more strains, such as Cellulomonas, Bacillus, Streptomyces, Trecodelma, Pseudomonas, etc., which produce antifungal substances. (Diffco) 's LB broth, Nutriunt broth's selected PDB or Merck's PDA medium and KCTC published in bacteria and fungi growth medium published in the Korea Advanced Institute of Science and Technology (KCTC) 2-3 days at 25 ~ 40 ℃ Incubate the microorganisms.

[제2공정](정균공정)[Step 2] (Bacterial Process)

제1공정에서 미생물 배양공정이 완료되면 이를 원심분리기에서 2,500~4,000rpm에서 균체를 수집하고 사상균의 경우에는 막(membrean)필터 또는 여과지를 이용하여 균체를 정균 또는 수집하여 배양액을 제조하거나 또는 수집된 균체를 360메쉬 이상의 이산화규소(SiO2), 폴리아크릴, 제오라이트, 맥반석, 점토 중 한종을 선택하여 부피비율로 30:70으로 혼합교반하고 건조시켜 배양분말을 제조한다.When the microbial culture process is completed in the first step, the cells are collected at a centrifuge at 2,500 to 4,000 rpm, and in the case of filamentous fungi, bacteria are collected or collected by using a membrane filter or a filter paper to prepare a culture medium or collected. The cell is prepared by mixing and stirring at least one of silicon dioxide (SiO 2 ), polyacryl, zeolite, elvan, and clay in a volume ratio of 30:70 and drying to obtain a culture powder.

[제3공정](부식산의 제조공정)[Step 3] (Manufacturing Process of Corrosive Acid)

부엽토, 부식토, 초탄, 이탄 또는 음식물 쓰레기등을 부식시킨 부식물질에 수산화나트륨(NaoH), 수산화칼륨(KOH), 염화칼슘(CaCl2) 중 선택하여 이를 물에 녹여 pH12 이상의 강알칼리수를 조성하고 부식물질과 강알칼리수를 중량비율로 30:70의 비율로 혼합하여 1시간 이상 교반하고, 24시간 이상 침전시킨 다음 침전물은 건조시켜 250~360메쉬 상태의 분말을 조성하여 1차 분말 부식산을 조성하고, 용액을 360메쉬 이상의 필터로 여과시킨 후 여과용액에 염산, 질산등을 이용하여 pH1~2가 되도록 조성한 후 다시 침전물과 용액을 분리시켜 침전물은 건조시켜 2차 분말 부식산을 제조하고, 용액은 물에 녹여 pH8~9로 조성하여 액상부식산을 제조한다.Choose from sodium hydroxide (NaoH), potassium hydroxide (KOH), calcium chloride (CaCl 2 ), and dissolve it in water to form a strong alkaline water of pH 12 or higher. And strong alkaline water in a ratio of 30:70 by weight ratio, stirred for 1 hour or more, precipitated for 24 hours or more, the precipitate was dried to form a powder of 250 ~ 360 mesh state to form a primary powder humic acid, The solution was filtered with a filter of 360 mesh or more, and then the filtrate was prepared to have a pH of 1 to 2 using hydrochloric acid or nitric acid. The precipitate was then separated from the solution, and the precipitate was dried to prepare a secondary powder humic acid. Dissolve in and prepare a pH 8 ~ 9 to prepare a liquid corrosion acid.

제4공정(미생물 조균과 액상 부식산의 혼합제품화 공정)4th process (mixed production process of microorganisms and liquid humic acid)

제3공정에서 제조된 액상부식산, 질산, 인산, 칼리를 중량비율로75:4~6:6~4:2~4:1을 혼합하여 붕소, 아연, 철, 구리등으로 보정하여 조성한 용액과 제2공정의 미생물 배양액을 90~98:10~2의 중량비율로 혼합하여 정량 포장하여 제품화 한다.The solution is prepared by mixing liquid corrosive acid, nitric acid, phosphoric acid, and kali prepared in the third process with a weight ratio of 75: 4 to 6: 6 to 4: 2 to 4: 1 and correcting it with boron, zinc, iron, and copper. Microbial culture solution of the second step and the mixture in a weight ratio of 90 ~ 98: 10 ~ 2 is quantitatively packaged and commercialized.

이 때 미생물 균주는 pH8~9의 알칼리 상에서 온도에 별다른 영향없이 생육을 하지않고 포자상태로 존재하게 된다.At this time, the microbial strain is present in spores without growing on the alkaline pH 8-9 without any effect on the temperature.

[제5공정](미생물 정균과 분말 부식산의 혼합제품화 공정)[Step 5] (Mixed Product Process of Microbial Bacteria and Powder Humic Acid)

제3공정에서 제조된 1차분말 부식산과 2차분말 부식산, 제2공정의 미생물 배양분말을 중량비율로 62~70:30~20:8~10을 혼합 균질화시켜 정량 포장하여 제품화 한다.The primary powder humic acid prepared in the third step, the secondary powder humic acid, and the microbial culture powder of the second step by mixing and homogenizing 62 ~ 70:30 ~ 20: 8 ~ 10 by weight ratio to be packaged and commercialized.

이와 같이 제조되는 본 발명의 미생물비료를 이용 과채류를 재배할때의 실시예를 설명하면 다음과 같다.Referring to the embodiment when cultivating fruit vegetables using the microbial fertilizer of the present invention prepared as described above are as follows.

[실시예1](무우, 배추 재배)Example 1 (beet, Chinese cabbage cultivation)

대구광역시 달성군 다사읍 문양리 소재 농지에서 환경변화가 균일한 비닐하우스의 내에서 서울종묘사의 무우와 배추의 종자를 선택하여 파종하였다.Radish and Chinese cabbage seeds were sown and planted in a vinyl house in Munyang-ri, Dasa-eup, Dalseong-eup, Daegu, Korea.

밭 만들기 과정에서 고랑에 복합비료(남해화학제품)를 기비로 시비한 다음 무처리구역에는 통상 재배와 같이 4종 복합 식물영양제, 액상석회, 미량요소제재를 물과 함께 시비하고, 처리구역에는 본 발명의 액제 500ml를 물에 40배로 희석하여 파종 후 7일 간격으로 2회를 엽면 시비하였다.In the process of making the field, fertilize the fertilizer with fertilizer (Namhae Chemicals) at the base, and then fertilize four complex plant nutrients, liquid lime, and trace urea with water in the untreated area, as in normal cultivation. 500 ml of the solution was diluted 40 times with water, and two times at 7 days intervals were sown after cultivation.

여기에서 파종후 8일이 경과되면 무처리구역에서는 발아율이 고르지 못하였고 처리구역간에는 고른 발아율을 보였으며 21일이 경료되자 배추와 무우 모두 생육에 현저한 차이를 나타내었다.The germination rate was uneven in the untreated area after 8 days after sowing, and evenly in the untreated area. After 21 days, both cabbage and radish showed significant difference in growth.

파종후 21일(무처리구역,배추) 파종후 21일(처리구역,배추)21 days after sowing (no treatment area, cabbage) 21 days after sowing (treatment area, cabbage)

파종후 21일(무처리역,무우) 파종후 21일(처리구역,무우)21 days after sowing (no treatment area, radishes) 21 days after sowing (treatment area, radishes)

또 본 발명에서 미생물 비료를 액상을 물에 희석하는 방법과 분말상태를 시비하는 방법을 각각 시행하였으나 그 결과는 비슷한 결과를 나타내었고 수확기에는 무처리구역보다는 처리구역에서 50% 이상 증산효과를 얻을 수 있었다.In addition, in the present invention, the method of diluting the liquid fertilizer in water and the method of fertilizing the powder state were carried out, respectively, but the results showed similar results, and the harvesting time was more than 50% in the treatment zone rather than the untreated zone. .

[실시예2](참외의 흰가루병 방제 실험)Example 2 (Melon Powder Control Experiment of Melon)

경상북도 성주군 선남면 도성리 소재의 참외 재배단지의 비닐하우스에서 흰가루병이 발생하였고 동 흰가루병은 일반공시 약제로는 방제에 어려움이 있어 미생물 비료의 균주가 흰가루병의 방제기능 또는 길항력을 갖는지를 실험하였다.The powdery mildew occurred in the plastic house of the melon cultivation complex in Doseong-ri, Seongju-myeon, Seongju-gun, Gyeongsangbuk-do. The powdery mildew was difficult to control with general public medicines.

흰가루병 원균은 식물기생균주로서 기내배양이 되지 않는 것으로 문헌에 보고되었으나 배지(기주식물유래성분의 배지)를 달리하여 기내배양을 하였고 이를 본 발명의 미생물 비료를 투여한바 흰가루병에 탁월한 길항력을 확인하였다.The powdery mildew progeny has been reported in the literature as a plant parasite, but the culture was carried out by changing the medium (medium of host plant-derived components) and confirmed the excellent antagonism to powdery mildew by administering the microbial fertilizer of the present invention. .

흰가루병원균의 기내배양된 상태(7일간 배양)In-flight culture of white powdery pathogen (7 days culture)

흰가루병에서 미생물 비료의 투여 후 7일 경과상태7 days after administration of microbial fertilizer in powdery mildew

또 병해시작 후 4일 경과후에 액상 미생물 비료를 40배의 비율로 하여 방제한 다음 처리구역과 무처리구역을 10일 경과후 비교하였던 바 무처리구역에서는 줄기가 연한 녹색으로 변화되면서 생장이 중단되어 고사중이었으나 처리구역에서는 흰가루병이 사멸되었고 생장이 계속되었음을 확인할 수 있었다.In addition, after 4 days after the start of the disease, the liquid microbial fertilizer was controlled at a ratio of 40 times, and the treated area and the untreated area were compared after 10 days. In the untreated area, the stem turned pale green and the growth was stopped. In the treatment area, however, powdery mildew was killed and growth continued.

병해발생 14일 상태 병해발생 4일 경과후 방제하고 10일경과후 상태14 days after disease occurred 10 days after disease occurred

이와 같이 과채류에 곰팡이 병해의 발생시 식물병원균이 내성기작을 획득하여 같은 약제를 계속 사용할 경우 방제효과를 얻을 수 없어 2~3종의 약제를 돌려가면서 방제하게 되나, 본 발명의 미생물 비료는 식물에 엽면살포(시비)를 하게 되면 미생물에서 생산되는 항진균성 물질이 곰팡이균의 생육을 억제 또는 사멸하게 되었고 부식산은 생장식물의 영양원으로 하여 뿌리의 발육과 생육에 도움을 주고 자가 면역력을 갖게 됨을 확인하였으며, 또 부식산은 유기물로서 토양속에 잔류하여 식물의 생장에 피해를 주는 비료염과 흡착되면서 미생물의 균주가 토양에서 작용하여 염류와 불용성 물질을 중화시키게 된다.As such, when the fungal disease occurs in fruit and vegetables, the plant pathogen acquires a resistance mechanism, and if the same drug is used continuously, control effects cannot be obtained. However, the microbial fertilizer of the present invention is foliar to the plant. When spraying (fertilizing), the antifungal substance produced by microorganisms inhibited or killed the growth of fungi, and the humic acid was a nutrient source for growth plants, which helped the development and growth of roots and had autoimmunity. Humic acid is an organic substance that remains in the soil and is adsorbed with fertilizer salts that damage plant growth. Microorganism strains act in the soil to neutralize salts and insoluble substances.

또 본 발명에서 분말 상태의 부식산은 강알칼리의 분말제이므로 산성화 된 토양에 사용하게 될 경우 토양을 알칼리화하게 되면서 토양과의 혼합으로 완충작용을 하여 토양에서의 급격한 pH변화를 막아주게 된다.In addition, the humic acid in the powder state in the present invention is a powder of strong alkali, so when used in the acidified soil while alkalizing the soil to prevent the rapid pH change in the soil by mixing with the soil.

또한 본 발명은 액상 미생물 비료는 용수탱크에서 혼합하여 관수로 시비하게 되면 비중변화가 없고 용해가 쉬워 사용이 편리하고 또 분말의 경우 토양에 시비하거나 식물에 엽면시비가 편리하게 되는 것이다.In addition, the present invention is liquid microbial fertilizer is mixed in the water tank and fertilized by irrigation, there is no change in specific gravity and easy to dissolve, and in the case of powder fertilized in the soil or plant foliar fertilization is convenient.

따라서 이상과 같은 본 발명은 길항미생물을 배양 정균시켜 이를 액상 또는 분말상태의 부식산과 혼합하여 미생물 비료를 제조하여 각종 농산물의 식물 재배시에 사용하게 되므로서 증산과 토양개량 및 성장식물의 면역력을 높일 수 있는 효과가 있는 유용한 발명이다.Therefore, the present invention as described above is used to cultivate antagonistic microbial culture and mixed with humic acid in the liquid or powder state to produce a microbial fertilizer to use in the cultivation of various agricultural plants, so as to increase the transpiration and soil improvement and the immunity of the growing plant It is a useful invention with the effect.

Claims (4)

항진균성 물질을 생산하는 미생물을 배양정균시킨 배양액 또는 배양분말과 부식물질에서 추출한 부식산액 또는 분말 부식산을 혼합하여 제조함을 특징으로 한 미생물과 부식산을 이용한 미생물 비료의 제조방법Method for producing microbial fertilizer using microorganisms and humic acid, characterized in that the culture medium or culture powder cultured and cultured microorganisms producing antifungal substances and mixed humic acid or powder humic acid extracted from the corrosive material 제1항에 있어서,The method of claim 1, 부식물질에 pH를 12 이상의 강알칼리수를 중량비율로 30:70으로 혼합하여 1시간 이상 교반하고 24시간 이상 침전시킨 후 침전물은 건조시켜 1차 분말 부식산을 조성하고, 용액은 360메쉬 이상의 필터로 여과시킨 후 염산 또는 질산을 이용하여 pH1~2가 되게 하여 2차 침전물은 건조시켜 2차 분말 부식산을 조성하고, 용액은 물을 이용 pH8~9로 조성한 액상부식산을 미생물과 혼합 제조됨을 특징으로 한 미생물과 부식산을 이용한 미생물 비료의 제조방법Strong alkali water with pH of 12 or more is mixed in a weight ratio of 30:70, stirred for at least 1 hour, precipitated for at least 24 hours, and the precipitate is dried to form a primary powdered humic acid. After filtering, the solution was dried to pH 1 ~ 2 using hydrochloric acid or nitric acid to dry the secondary precipitate to form secondary powder humic acid, and the solution was prepared by mixing liquid corrosive acid with pH 8-9 using microorganisms. Of microbial fertilizer using microorganism and humic acid 제1항에 있어서,The method of claim 1, 액상부식산, 질소, 인산, 알칼리를 중량비율로 75:4~6:6~4:2~4로 혼합한 용액과 미생물 배양액을 90~98:10~2의 중량비율로 혼합함을 특징으로 한 미생물과 부식산을 이용한 미생물 비료의 제조방법It is characterized by mixing the solution of the liquid corrosion acid, nitrogen, phosphoric acid, alkali in the weight ratio of 75: 4-6: 6-4: 2-4 and the microbial culture at a weight ratio of 90-98: 10-2 Manufacturing method of microbial fertilizer using a microorganism and humic acid 제1항에 있어서,The method of claim 1, 1차분말 부식산과 2차분말부식산 및 미생물 배양분말을 중량비율로 62~70:30~20:8~10으로 혼합 균질화시켜 제조됨을 특징으로 한 미생물과 부식산을 이용한 미생물 비료의 제조방법Method for producing microbial fertilizer using microorganisms and humic acid, characterized in that the first powder is prepared by mixing and homogenizing humic acid, secondary powder corrosion acid and microbial culture powder in a weight ratio of 62 ~ 70:30 ~ 20: 8 ~ 10
KR10-2002-0024054A 2002-05-02 2002-05-02 The microbe fertilizer's producting method be used by microbe and humic acid KR100462953B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR10-2002-0024054A KR100462953B1 (en) 2002-05-02 2002-05-02 The microbe fertilizer's producting method be used by microbe and humic acid

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR10-2002-0024054A KR100462953B1 (en) 2002-05-02 2002-05-02 The microbe fertilizer's producting method be used by microbe and humic acid

Publications (2)

Publication Number Publication Date
KR20020036830A KR20020036830A (en) 2002-05-16
KR100462953B1 true KR100462953B1 (en) 2004-12-23

Family

ID=19720564

Family Applications (1)

Application Number Title Priority Date Filing Date
KR10-2002-0024054A KR100462953B1 (en) 2002-05-02 2002-05-02 The microbe fertilizer's producting method be used by microbe and humic acid

Country Status (1)

Country Link
KR (1) KR100462953B1 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170038453A (en) 2015-09-30 2017-04-07 효성오앤비 주식회사 Compound fertilizer composition
KR102372764B1 (en) 2020-12-11 2022-03-10 주식회사 원천환경기술 Manufacturing method of compost for urban using cow manure as the main raw material

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100638739B1 (en) * 2003-12-26 2006-10-25 한국전자통신연구원 Utility function prediction apparatus for real-time multimedia contents transcoding, and multimedia contents transcoding access system using it
RU2480224C2 (en) * 2011-01-12 2013-04-27 Государственное бюджетное образовательное учреждение высшего профессионального образования "Самарский государственный медицинский университет" Министерства здравоохранения и социального развития Российской Федерации (ГБОУ ВПО СамГМУ Минздравсоцразвития России) Method for preparing biologically active substances of humic acids of low-mineralised silt sulphide mud
CN104961538B (en) * 2015-05-08 2017-11-14 淮阴工学院 Recessed soil matrix immobilization complex micro organism fungicide for improveing capsicum continuous cropping obstacle and preparation method thereof
RU2678204C1 (en) * 2018-05-21 2019-01-24 Федеральное государственное бюджетное образовательное учреждение высшего образования "Самарский государственный медицинский университет" Министерства здравоохранения Российской Федерации Method of obtaining preparation with hepatoprotective activity

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58208191A (en) * 1982-05-27 1983-12-03 株式会社協立有機工業研究所 Soil activator
US4952229A (en) * 1983-09-27 1990-08-28 Hugh M. Muir Plant supplement and method for increasing plant productivity and quality
KR960010587A (en) * 1994-09-29 1996-04-20 이토 겐스케 Silver Sintered Product and Manufacturing Method Thereof
KR970006241A (en) * 1995-07-24 1997-02-19 쇼다 오사무 Manufacturing method of organic fertilizer
JPH1067590A (en) * 1996-08-23 1998-03-10 Nissan Chem Ind Ltd Material for growing plant
KR20020042013A (en) * 2000-11-29 2002-06-05 양재의 Method and apparatus for fluid fertilizer manufacture from Organic Industrial Wastes

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS58208191A (en) * 1982-05-27 1983-12-03 株式会社協立有機工業研究所 Soil activator
US4952229A (en) * 1983-09-27 1990-08-28 Hugh M. Muir Plant supplement and method for increasing plant productivity and quality
KR960010587A (en) * 1994-09-29 1996-04-20 이토 겐스케 Silver Sintered Product and Manufacturing Method Thereof
KR970006241A (en) * 1995-07-24 1997-02-19 쇼다 오사무 Manufacturing method of organic fertilizer
JPH1067590A (en) * 1996-08-23 1998-03-10 Nissan Chem Ind Ltd Material for growing plant
KR20020042013A (en) * 2000-11-29 2002-06-05 양재의 Method and apparatus for fluid fertilizer manufacture from Organic Industrial Wastes

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20170038453A (en) 2015-09-30 2017-04-07 효성오앤비 주식회사 Compound fertilizer composition
KR102372764B1 (en) 2020-12-11 2022-03-10 주식회사 원천환경기술 Manufacturing method of compost for urban using cow manure as the main raw material

Also Published As

Publication number Publication date
KR20020036830A (en) 2002-05-16

Similar Documents

Publication Publication Date Title
CN100579940C (en) Method for preparing biologic organic fertilizer for preventing and curing eelworm harm and use thereof
KR101669599B1 (en) Composition for eliminating odor and heavy metal comprising effective microorganism culture broth as effective component
CN101875571B (en) Method for preparing enhanced liquid microbial organic fertilizer
CN107285926A (en) A kind of compound organic and inorganic fertilizer of prevention and control soil-borne disease and preparation method thereof
CN110305671A (en) One kind is for facilities vegetable continuous cropping soil biological modification agent and its preparation and application method
CN106588424A (en) Controlled release fertilizer special for saline alkali soil improvement
CN107793267A (en) One kind is for sulfate alkaline land improving microbial-bacterial fertilizer and preparation method thereof
CN104788192A (en) Special fertilizer for rice seedling raising
CN105850262A (en) Method for planting crops on saline and alkaline land
CN104285533A (en) Method for using agricultural straw to improve saline-alkali soil
CN104782261A (en) Method for improving greenhouse strawberry continuous cropping soil
CN107382496A (en) A kind of artificial soil for strengthening crop anti-adversity and the method for strengthening crop anti-adversity
CN109496758A (en) A kind of disease is inoculated with reparation and the nursing method of tobacco field
CN108129238A (en) A kind of multi-functional enzyme liquid and preparation method thereof
CN103193534A (en) Production technology of dry raising strong rice seedling biological matrix
CN105418206A (en) Enzyme type dryland-raised rice seedling matrix and preparation method thereof
CN102276337A (en) Re-cultivation long-acting bio-organic fertilizer
KR100462953B1 (en) The microbe fertilizer's producting method be used by microbe and humic acid
CN109020651A (en) The fast well-fattened special fertilizer and the preparation method and application thereof of salinization of soil farmland tillaging layer soil
CN105175159A (en) Production method of rice seedling dry raising and strengthening biological medium and application thereof
US20220315504A1 (en) Regulating solution, fertilizer and soil conditioner for increasing vitamin c content of plants as well as preparation and application thereof
CN105565915A (en) Anti-grub soil conditioning biological fertilizer and production method thereof
KR100430761B1 (en) Method for producing high-density antagonistic microbes base material
CN108949635A (en) Bacillusmusilaginosiengineering, microbial-bacterial fertilizer, preparation method and its application
CN104557236A (en) Continuous cropping obstacle resisting agent for largehead atractylodes rhizome

Legal Events

Date Code Title Description
A201 Request for examination
E902 Notification of reason for refusal
E701 Decision to grant or registration of patent right
GRNT Written decision to grant
FPAY Annual fee payment

Payment date: 20121204

Year of fee payment: 9

FPAY Annual fee payment

Payment date: 20131121

Year of fee payment: 10

FPAY Annual fee payment

Payment date: 20150113

Year of fee payment: 11

FPAY Annual fee payment

Payment date: 20160113

Year of fee payment: 12

FPAY Annual fee payment

Payment date: 20170313

Year of fee payment: 13

FPAY Annual fee payment

Payment date: 20171213

Year of fee payment: 14

FPAY Annual fee payment

Payment date: 20181203

Year of fee payment: 15