KR100425529B1 - Process for production of letters and characters using moss tissue culture - Google Patents
Process for production of letters and characters using moss tissue culture Download PDFInfo
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- KR100425529B1 KR100425529B1 KR10-2001-0042271A KR20010042271A KR100425529B1 KR 100425529 B1 KR100425529 B1 KR 100425529B1 KR 20010042271 A KR20010042271 A KR 20010042271A KR 100425529 B1 KR100425529 B1 KR 100425529B1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/22—Improving land use; Improving water use or availability; Controlling erosion
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- Developmental Biology & Embryology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
본 발명은 이끼의 조직배양을 이용한 문자 및 그림 캐릭터 생산방법에 관한 것으로, 이끼Physcomitrella patens를 멸균용기에서 조직배양하여 문자 및 그림 캐릭터를 생산하는 방법에 관한 것이다.The present invention relates to a method of producing a character and a picture character using tissue culture of moss, and a method of producing a character and a picture character by tissue culture of the moss Physcomitrella patens in a sterile container.
Description
본 발명은 이끼의 조직배양을 이용한 문자 및 그림 캐릭터 생산방법에 관한것으로, 더욱 상세하게는 이끼Physcomitrella patens를 조직배양한 다음 문자나 그림 캐릭터 형상으로 본뜨고, 이를 멸균용기에서 생장시켜 문자나 그림 캐릭터로 생산하는 방법에 관한 것이다.The present invention relates to a method of producing a character and a picture character using the tissue culture of moss, and more specifically, to moss Physcomitrella patens tissue culture and then modeled in the form of a character or a picture character, it is grown in a sterile container letter or a picture character To produce as.
문화수준이 높아짐에 따라서 꽃꽃이나, 화분 등의 식물 판매가 증대하고 있다. 식물의 시판은 카네이션, 장미, 안개꽃 등 생화를 절화하여서 판매하는 경우와 동양란과 양란 등 생화 형태로 판매가 되고 있다. 또한 조직배양된 식물들이 판매되는 경우가 있으나, 아직 식물체를 문자나 그림의 형태로 만들어서 시판되는 경우는 세계적으로 상용화된 바가 없다.As the level of culture increases, the sales of plants such as flowers and pots are increasing. Commercially available plants are sold in the form of cut flowers such as carnations, roses and gypsophila, and in the form of flowers such as oriental eggs and poached eggs. In addition, tissue-cultivated plants may be sold, but there are no commercially available plants in the world in the form of letters or pictures.
한편, 이끼는 기본영양배지에서 조직배양이 용이할 뿐만 아니라 생장의 속도도 극히 빠르며, 초기에는 얇은 잔디처럼 생장을 시작하나 후반에는 mini plant의 형태로 작은 잎사귀 모양으로 자란다.On the other hand, moss not only facilitates tissue culture in the basic nutrient medium, but also grows extremely fast, and grows like a thin grass in the beginning, but grows in the shape of a small leaf in the form of a mini plant in the second half.
본 발명자는 상기와 같은 점에 착안하여 이끼Physcomitrella patens를 조직배양한 다음 문자나 그림 캐릭터 형상으로 본뜨고, 이를 멸균용기에서 생장시킴으로써 식물을 재배하는 취미생활을 즐길 수 있도록 할 뿐만 아니라 실내에 장식적인 효과를 극대화 할 수 있는 문자 및 그림 캐릭터를 제조함으로써 본 발명을 완성하였다.The present inventors focus on the above points, and then culture the moss Physcomitrella patens and then emulate them in the form of letters or pictograms, and grow them in sterilized containers to enjoy the hobby of planting plants as well as to decorate them indoors. The present invention has been completed by manufacturing a character and a figure character that can maximize the effect.
따라서, 본 발명의 목적은 이끼의 조직배양을 이용한 문자 및 그림 캐릭터 생산방법을 제공함에 있다.Accordingly, it is an object of the present invention to provide a character and a figure character production method using the tissue culture of moss.
본 발명의 다른 목적은 상기방법에 의해 제조된 조직배양된 이끼로 구성되는 문자나 그림 캐릭터를 제공함에 있다.Another object of the present invention is to provide a character or a figure character consisting of tissue cultured moss prepared by the method.
본 발명의 상기 목적은 이끼Physcomitrella patens를 Knops 배지에서 조직배양한 다음 문자나 그림 캐릭터 형상의 모형판으로 찍어내고, 이를 멸균용기에서 생장시켜 문자나 그림 캐릭터로 생산함으로써 달성하였다.The above object of the present invention was achieved by tissue culture of moss Physcomitrella patens in Knops medium and then imprinted with a model plate of character or figure character shape, and grown in a sterile container to produce a character or figure character.
이하, 본 발명의 구성을 설명한다.Hereinafter, the configuration of the present invention will be described.
도 1은 이끼 조직배양 10일 후의 모습을 나타낸 사진이다.1 is a photograph showing a state after 10 days of cultured moss tissue.
도 2는 조직배양 후 4주 후의 이끼 모습을 나타낸 사진이다.Figure 2 is a photograph showing the state of moss after 4 weeks after tissue culture.
도 3은 이끼를 조직배양하여 생산하는 본 발명 그림 캐릭터의 일실시예를 나타낸 사시도이다.Figure 3 is a perspective view showing an embodiment of the figure character of the present invention produced by tissue culture of moss.
도 4는 조직배양한 이끼를 문자나 그림 모양으로 본뜬 다음 Knops 배지에 다시 옮겨 넣었을 때의 가상 형태를 나타낸 그림이다.4 is a diagram showing a virtual form when tissue cultured moss is modeled after a letter or a figure and then transferred to Knops medium again.
〈도면의 주요부분에 대한 부호의 설명><Explanation of symbols for main parts of the drawings>
10 : 멸균용기 20 : 이끼의 조직배양용 배지10: sterilization container 20: culture medium for moss
30 : 이끼 40 : 멸균용기의 뚜껑30: lichen 40: lid of the sterilization container
본 발명은 기내배양 이끼인physcomitrella patens를 유한락스 100㎖에 tween 20 2∼3방울 첨가한 희석한 유한락스 수용액에서 소독하고 멸균수로 세척하고, 상기 세척한 이끼에 멸균수를 넣어 blender로 마쇄하는 단계; 상기 마쇄된 이끼를 MgSO4-7H2O 250 mg/L, KH2PO4250 mg/L, KNO31g/L, FeSO4-7H20 12.5 mg/L, NH4tartrate 920 mg/L, CaCl2147.02 mg/L, Nicotinic acid 1 mg/L,P-aminobenzoic acid 250 ng/L, Thiamine HCl 0.5 mg/L이 함유된 Knops 고체 배지(pH 5.8)에 0.5㎝ 두께로 분주하여 10일 동안 배양하는 단계; 문자 또는 그림 캐릭터 모양의 본뜨는 형판으로 찍어낸 다음 2∼3㎝의 Knops 고체배지가 첨가되어 있는 멸균 용기 내에서 생장시키는 단계로 구성된다.The present invention is to disinfect physcomitrella patens , a cultured moss, in 100 ml of finite lacquer in diluted finite lacquer solution added with 2 to 3 drops of tween 20, washed with sterile water, and sterile water is put into the washed moss and ground with a blender. step; Wherein the grinding moss MgSO 4 -7H 2 O 250 mg / L, KH 2 PO 4 250 mg / L, KNO 3 1g / L, FeSO 4 -7H 2 0 12.5 mg / L, NH 4 tartrate 920 mg / L, Incubate for 10 days by dispensing 0.5cm thick in Knops solid medium (pH 5.8) containing CaCl 2 147.02 mg / L, Nicotinic acid 1 mg / L, P- aminobenzoic acid 250 ng / L, Thiamine HCl 0.5 mg / L Doing; The pattern of the character or figure character is stamped into a template and grown in a sterile container to which a 2-3 cm Knops solid medium is added.
본 발명에서 Knops 고체배지의 조성은 MgSO4-7H2O은 150∼350 mg/L 바람직하게는 250 mg/L이고, KH2PO4는 150∼350 mg/L 바람직하게는 250 mg/L이고, KNO3은0.1∼10g/L 바람직하게는 1g/L이고, FeSO4-7H20는 10∼20 mg/L, 바람직하게는 12.5 mg/L이고, NH4tartrate은 800∼1000 mg/L, 바람직하게는 920 mg/L이고, CaCl2은 100∼200 mg/L, 바람직하게는 147.02 mg/L이고, Nicotinic acid는 0.1∼10 mg/L, 바람직하게는 1 mg/L이고,P-aminobenzoic acid는 200∼300 mg/L 바람직하게는 250ng/L이고, Thiamine HCl은 0.1∼1 mg/L 바람직하게는 0.5 mg/L이며, pH는 5∼7, 바람직하게는 pH 5.8의 Knops 고체배지를 사용한다.In the present invention, the composition of the Knops solid medium is MgSO 4 -7H 2 O is 150 to 350 mg / L preferably 250 mg / L, KH 2 PO 4 is 150 ~ 350 mg / L preferably 250 mg / L , KNO 3 is 0.1~10g / L preferably from 1g / L, FeSO 4 -7H 2 0 is 10~20 mg / L, preferably 12.5 mg / L, NH 4 tartrate is 800~1000 mg / L , Preferably 920 mg / L, CaCl 2 is 100-200 mg / L, preferably 147.02 mg / L, Nicotinic acid is 0.1-10 mg / L, preferably 1 mg / L, P − Aminobenzoic acid is 200-300 mg / L preferably 250ng / L, Thiamine HCl is 0.1-1 mg / L preferably 0.5 mg / L, Knops solid medium at pH 5-7, preferably pH 5.8 Use
본 발명 이끼의 조직배양을 이용한 문자 및 그림 캐릭터 생산방법의 바람직한 실시예는 도 3에 도시한 바와 같이, 투명 멸균용기(10)상에 분주된 이끼의 조직배양용 배지(20)과 그 위에 배양되는 이끼(30) 및 멸균용기의 뚜껑(40)으로 구성됨을 특징으로 한다.According to a preferred embodiment of the method for producing a character and a figure character using the tissue culture of the moss of the present invention, as shown in Figure 3, the culture medium for culture of the moss dispensed on the transparent sterilization container 10 and cultured thereon Characterized by consisting of the lid 40 of the moss 30 and the sterilization container.
이하, 본 발명의 구체적인 방법을 실시예를 들어 상세히 설명하고자 하지만,본 발명의 권리범위는 이들 실시예에만 한정되는 것은 아니다.Hereinafter, the specific method of the present invention will be described in detail with reference to Examples, but the scope of the present invention is not limited only to these Examples.
실시예 : 이끼의 조직배양을 이용한 문자 및 그림 캐릭터 생산Example: Production of Characters and Pictographs Using Tissue Culture of Moss
기내배양 이끼인physcomitrella patens를 유한락스 100㎖에 tween 20 2∼3방울 첨가한 희석한 유한락스 수용액에서 소독하고 멸균수로 세척하고, 상기 세척한 이끼에 멸균수를 넣어 blender로 마쇄하였다. Physcomitrella patens , an in-flight cultured moss, was sterilized in a diluted aqueous solution of finite lacx in which tween 20 2-3 drops were added to 100 ml of finite lacquer , washed with sterile water, and sterile water was added to the washed moss and ground with a blender.
상기 마쇄한 이끼를 상기 마쇄된 이끼를 MgSO4-7H2O 250 mg/L, KH2PO4250 mg/L, KNO31g/L, FeSO4-7H20 12.5 mg/L, NH4tartrate 920 mg/L, CaCl2147.02mg/L, Nicotinic acid 1 mg/L,P-aminobenzoic acid 250 ng/L, Thiamine HCl 0.5 mg/L이 함유된 하기 표 1의 조성비를 갖는 Knops 고체 배지(pH 5.8)상에 분주한 후에 10 일 생장시켰다. 상기 생장된 이끼는 미리 준비된 문자 및 그림 모양의 본뜨는 형판으로 찍어내어서, 2∼3 cm의 Knops 고체 배지가 첨가되어 있는 멸균용기내에 넣어서 생장시켰다.The ground moss was used as MgSO 4 -7H 2 O 250 mg / L, KH 2 PO 4 250 mg / L, KNO 3 1g / L, FeSO 4 -7H 2 0 12.5 mg / L, NH 4 tartrate 920 mg / L, CaCl 2 147.02 mg / L, Knops solid medium (pH 5.8) having a composition ratio of Table 1 containing 1 mg / L Nicotinic acid, 250 ng / L P- aminobenzoic acid, 0.5 mg / L Thiamine HCl 10 days after dispensing). The grown moss was photographed with a template prepared in the form of letters and pictures prepared in advance, and grown in a sterile container to which Knops solid medium of 2-3 cm was added.
도 1은 이끼 조직배양 10일 후의 모습을 나타낸 사진도이며, 도 2는 조직배양 4주 후의 이끼 모습을 나타낸 사진도이다.1 is a photograph showing a state after 10 days of moss tissue culture, Figure 2 is a photograph showing a state of moss after 4 weeks of tissue culture.
이상, 상기 실시예를 통하여 설명한 바와 같이 본 발명은 이끼를 Knops 고체배지에서 조직배양함으로써, 식물을 재배하는 취미생활을 즐길 수 있을 뿐만 아니라, 다양한 문자나 그림 캐랙터 모양으로 장식할 수 있어 환경미화적 장식품으로써 효과가 있고, 별도의 관수장치가 없이도 간편하게 식물을 재배할 수 있어 식물재배산업상 매우 유용한 발명인 것이다.As described above, the present invention can not only enjoy the hobby of cultivating plants, but also decorate it in various letters or picture character shapes by tissue culture of moss in a Knops solid medium. It is effective as a decoration, it is a very useful invention in the plant cultivation industry because the plant can be grown easily without a separate watering device.
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KR100701978B1 (en) * | 2004-09-21 | 2007-03-30 | (주)엔피피 | Accessory Using Plant Itself, Root or Seed Thereof |
KR100720956B1 (en) * | 2005-04-18 | 2007-05-22 | (주)엔피피 | Fancy Product Using Plant |
CN106359092B (en) * | 2016-08-30 | 2018-12-14 | 中国科学院昆明植物研究所 | Small liwan moss protonema rapid propagation method |
KR102580907B1 (en) * | 2022-09-30 | 2023-09-21 | 주식회사 인자락 | Method And Apparatus For Stone Breeding Moss |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
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KR880010658A (en) * | 1987-03-13 | 1988-10-24 | 고갑천 | Test tube garden |
JPH02234902A (en) * | 1989-02-10 | 1990-09-18 | Maria L Fletcher | Decorative material and preparation thereof |
JPH05268843A (en) * | 1992-03-24 | 1993-10-19 | Hiraoka Kankyo Kagaku Kenkyusho | Cultured species of moss and method for cultivating mosses using the same |
JPH0766A (en) * | 1993-06-18 | 1995-01-06 | Hiraoka Kankyo Kagaku Kenkyusho | Moss ball and method for culturing the same |
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Publication number | Priority date | Publication date | Assignee | Title |
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KR880010658A (en) * | 1987-03-13 | 1988-10-24 | 고갑천 | Test tube garden |
JPH02234902A (en) * | 1989-02-10 | 1990-09-18 | Maria L Fletcher | Decorative material and preparation thereof |
JPH05268843A (en) * | 1992-03-24 | 1993-10-19 | Hiraoka Kankyo Kagaku Kenkyusho | Cultured species of moss and method for cultivating mosses using the same |
JPH0766A (en) * | 1993-06-18 | 1995-01-06 | Hiraoka Kankyo Kagaku Kenkyusho | Moss ball and method for culturing the same |
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