JPH02238829A - Raising seedling of dalmatian pyrethrum - Google Patents

Abstract

PURPOSE:To accomplish mass production of Dalmatian pyrethrum by making a culture of thin fruits or petals of red-flowered Dalmatian pyrethrum in a specific medium for plant regeneration and by proliferation of the resulting regenerated plant to enable the plant to be directly regenerated from the thin fruits or petals. CONSTITUTION:Thin fruits or petals of red-flowered Dalmatian pyrethrum are put to culture, under irradiation (pref. at 500-20000 lux), in a medium for plant regeneration containing an auxin and cytokinin to directly regenerate plants which are then put to culture in a proliferative medium to effect prolifera tion of numerous plants. It is preferable that said medium for regeneration contain 10mg/l of the auxin and 0.02-10mg/l of the cytokinin and said medium for proliferation contain 0.02-10mg/l of the cytokinin.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Application Field) The present invention relates to a method for raising seedlings of red-flowering pyrethrum, particularly to a method for obtaining a large amount of plants by culturing petals or achenes. (Prior art) Pyrethrum continues to be cultivated mainly in Africa because its fruit contains pyrethrin, an active insecticidal ingredient. Attempts have been made to produce pyrethrin through tissue culture of pyrethrum, but the amount produced is extremely small and industrialization has not yet been achieved, and cultivation continues in the field. For this reason,
It is necessary to raise seedlings of superior varieties and mass-produce seedlings, and methods such as dividing the plant, culturing the upper part of the plant, and regenerating the plant from callus are used, but it is not possible to directly obtain seedlings from petals or achenes. unknown. (Problem B to be Solved by the Invention) An object of the present invention is to provide a method for obtaining a large amount of pyrethrum seedlings in a short period of time using the petals or achenes of pyrethrum. (Means for Solving the Problems) The present invention relates to red flowering pyrethrum (Chrysanthenus +
cocci-neum) in a plant regeneration medium containing auxins and cytokinins under light irradiation to directly regenerate the plant; This invention relates to a method for raising red-flowering pyrethrum seedlings, which includes the steps of culturing and propagating a large number of plants. According to the present invention, the petals or achenes of red-flowered pyrethrum are first sterilized, cultured in a plant regeneration medium under light irradiation, and the plants are regenerated directly from the petal sections or the walls of the achenes without inducing callus. ．． As the plant regeneration medium, Murashige-Skoog (MS, Table 1) medium, White's medium, etc., which are commonly used for plant tissue nourishment, are used, and auxins and auxins are added to this to promote plant regeneration. Contains cytokinins. Table 1 Components of Murashige-Skoog medium Auxins include naphthalene acetic acid (NAA),
Examples include 2.4-dichlorophenoxyacetic acid (2.4-D) indoleacetic acid (IAA). Examples of cytokinins include penzyladenine (BA), kinetin (K
n) etc. Auxins are usually 10 Ilg/l or less, preferably 0.02 to 10 mg/j! The proportion of cytokinins is usually 0.02 to 10 mg/11, preferably 0.2 to 2.0 mg/l, particularly preferably 0.2 to 2.0 mg/l.
It is contained in the medium at a ratio of 2 to 10 g/β of steel. If the amount of these plant hormones is too low, it will be difficult to induce the plant body. Cultivation is carried out aerobically, with a culture temperature of 15-3
5°C, preferably 20-30°C, culture pH 4-7.
5 and around 5.7 is desirable. 500 to 2 as light illuminance
0,001ux, preferably 1.000 to 3,0
When culturing is carried out under a cycle of 16 hours of irradiation and 8 hours of darkness at 001 ux, plants are formed on the cut ends of petals or on the surfaces of achenes after 1 to 4 weeks. As the plant growth medium, the above-mentioned plant regeneration medium can be commonly used. To promote proliferation, cytokinins,
For example, BASKn is added. These concentrations are usually 0.02 to 10B/j! , preferably 2.0 to 10 m
g/j! Used in. After rooting, the seedlings grown on a solid medium can be transplanted into regular soil and grown as normal plants.
In addition, this regenerated plant contains the same level of insecticidal active ingredients as the parent plant. (Examples) Hereinafter, the present invention will be explained in more detail with reference to Examples. Example 1 Petals or achenes of red-flowering pyrethrum were immersed in 70% ethanol for 20 seconds, further immersed in an aqueous antiformin solution with an effective chlorine concentration of 3% for 20 minutes, and then sterilized by washing thoroughly with sterilized water. B A2. O+mg/l, N A A
Placed on a medium containing 0.2 mg/l and solidified with 1% agar, irradiated with light (20001 ux) at 24°C for 16 hours,
The cells were incubated in the dark for 8 hours. Plants are regenerated directly from the cut end of the petal or the surface of the achenes within one to four weeks. When the regenerated seedlings are separated and cultured on an agar medium prepared by adding only 2.0 mg of BA to the MS medium having the composition shown in Table 1, a larger number of plants appear. Furthermore, when these seedlings are cultured on hormone-free MS medium, they will root and become complete plants. (Effects of the Invention) According to the present invention, a plant body can be directly regenerated from the petals or achenes of red-flowering pyrethrum, and young plants of pyrethrum can be mass-produced. Patent applicant: Pesticide Biotechnology Development Technology Research Association

Claims (1)

[Claims] 1. Red-flowered pyrethrum (Chrysanthemumcocc)
ineum) under light irradiation using a plant regeneration medium containing auxins and cytokinins to directly regenerate the plant; cultivating the regenerated plant using a growth medium; A method for raising red-flowering pyrethrum seedlings, which includes the steps of: and propagating a large number of plants. 2. The method according to item 1, wherein the plant regeneration medium contains auxins at a ratio of 10 mg/l or less and cytokinins at a ratio of 0.02 to 10 mg/l. 3. The growth medium contains 0.02 to 10 m of cytokinins.
The method according to paragraph 1, containing g/l. 4. The method according to item 1, wherein the irradiation light is 500 to 20,000 lux.