KR100701978B1 - Accessory Using Plant Itself, Root or Seed Thereof - Google Patents

Abstract

The present invention relates to an accessory using a plant, and more particularly, comprising a culture vessel including a cultured plant dwarfized by culture medium and tissue culture, and a sealing member for sealing the culture vessel in a sterile state. It relates to an accessory using a plant. The accessory of the present invention eliminates the hassle of supplying water or nutrients periodically by sterilely culturing the cultured plants, culture roots or culture seeds cultivated in a transparent container in a medium in which various colors are expressed, By varying the shape of the culture vessel, the pigment added to the medium has the advantage that can improve the visual value.

Tissue Culture, Plants, Media, Accessories

Description

Accessories Using Plant Itself, Root or Seed Thereof}

1 is a photograph of the accessories cultured culture plants of the present invention

Figure 2 is a photograph of the accessories cultured culture root of the present invention

Figure 3 is a photograph of the accessories incubated culture culture seed of the present invention

The present invention relates to an accessory using a plant, and more particularly, to improve visual and decorative value by using a cultured plant, a culture root or a culture seed that has been dwarfized through tissue culture, and a living plant, root or seed without special management. It relates to accessories using.

In general, plants need to be periodically supplied with moisture and nutrients necessary for growth by growing them in pots, so miniaturizing them and using them as accessories is very difficult and almost impossible.

In recent years, plant tissue culture technology has helped to cultivate in-vehicle cultures in medium in transparent containers, thereby eliminating the need to periodically supply water or other nutrients to plants, but it is inappropriate for accessory in terms of size and visual aspects. It was just a fancy plant cultivation.

Recently, however, a method for accelerating plants has been published by growing in-flight cultured plants in a transparent container medium through plant tissue culture technology (Korean Patent Publication No. 10-2004-0013593). Specifically, in the patent, the storage member is stored so that the seeds can germinate on one surface; A sealing member inserted and fixed to one surface of the storage member and provided with its own elastic force; And close to the sealing member discloses an accessory provided with a germination chamber, characterized in that consisting of a closure member is inserted and fixed to form a germination chamber in which the seeds germinate on one surface of the storage member, which is a living seed therein When germination meets germination conditions, the electronic energy of the human body, the magnetic field of the magnet member, and the gold material are composed electronically with the electronic organic matter of the sprout. It is characterized by having a circuit to transition to the human body. In addition, by using a transparent finishing member, the growth process of sprouts can be seen from the outside, so you can enjoy freshness from various angles, and in pure nature without any artificial elements to transfer the plant bioenergy radiating to the human body when sprouts germinate. The energy gained provides the effect of contributing to the human health and youth therapies the natural, friendly energy of the seed from which the seed is born. However, the patent eliminates the trouble of periodically supplying water or other nutrients to the plant, but the structure is complicated, such as a germination chamber, and it is inappropriate to be accessory in size and visual parts, and thus for cultivating ornamental plants. It was only a fancy.

The present invention has been made in order to solve the problems of the accessory using the conventional plants as described above and to satisfy the various needs of modern people pursuing well-being, the sterile culture plants, culture roots or culture seeds through tissue culture in a sterile state It is an object to provide an accessory containing a plant having the advantage of observing the growth process of the plant without any additional management while wearing by putting in a small transparent container and accessories such as a cell phone hanger, necklace, earrings.

In order to achieve the above object, the present invention provides an accessory using a plant comprising a culture vessel comprising a culture medium and a culture plant and a sealing member for sealing the culture vessel in a sterile state.

Hereinafter, the present invention will be described in detail.

In the present invention, the tissues of plants are cultured for 2 to 6 weeks under photoperiod conditions of 12 to 16 hours at a roughness of 2,000 to 2,500 lux using a culture medium to which sucrose and gelrite are added. By inducing shoots by using the plant characterized in that it comprises a dwarfed plant formed by subcultured under the conditions of cancer culture, a culture vessel containing the same and a sealing member for sealing the culture vessel in a sterile state. Provide accessories.

In the above, as the culture plants, dwarfized plants themselves, culture roots or culture seeds may be used. Dwarfed plants suitable for the present invention are not only capable of growing without supply of water, but also have a slow growth rate so that they can grow inside the main body of the culture vessel, and must be possible as accessories. Succulent plants are suitable as plants that satisfy these conditions. . Succulents are plants that store a large amount of moisture on the stems and leaves of the ground to grow naturally in low moisture areas such as deserts and high mountains and to withstand dryness, such as ballus, laurie, and African violet. Slow and adaptable to moisture and nutrient depletion, it is one of the plants suitable for this purpose. Since the seed should also be suitable for the above conditions, it is preferable to use seeds obtained from the open field, that is, seeds or somatic embryos obtained through culturing or tissue culture, and in the case of roots, the seed can be grown in the medium used in the present invention. Wild ginseng, ginseng, daily herb, Astragalus, jichi, hairy root or irregular root obtained through tissue culture such as Shiho is suitable.

In manufacturing the accessory of the present invention, the plant included in the accessory is characterized in that the dwarfized plant. The non-dwarfed general plants must be supplied with moisture for growth, and in general, the growth rate is too fast to grow in the container so that it cannot grow inside the container body. Cannot be used. Therefore, in order to be used in the accessories of the present invention, plants such as succulents, which have these characteristics inherently, or they must be used after transforming them into plants having the above characteristics through the process of dwarfization to ordinary plants. Any plant can be used as long as it meets the above conditions. In the present invention, by using the dwarfized plants as described above, the problems that the accessories using the conventional plants could not solve.

In preparing the accessory including the plant of the present invention, the plant leaf, the culture root or the culture seed is 2,000 to 2,500 lux in 12 to 16 hours photoperiod condition using MS culture medium to which sucrose and gellite are added. After induction of shoots by incubation for 2 to 6 weeks, it is subcultured to cancer culture conditions again to form dwarfed plants and then used for the preparation of accessories. At this time, the culture temperature is preferably about 25 ℃, the pH of the medium is preferably 5.8, it is preferable to use a medium to which auxin including 2,4-D is added when culturing the culture root. In a preferred embodiment of the present invention, MS culture medium added with 3% sucrose and 0.5% gelite was used.

As the culture medium, MS (Murashige and skoog) medium which is widely used in cultivating plant tissues may be used, and in addition to this, medium such as B5, white, white, N6, SH, and Anderson may be used. By adding various food coloring to the plant, it is possible to express various colors without harming the growth of plants, etc., to meet various tastes of consumers. The MS medium is a medium having a high total inorganic ion concentration designed for tobacco callus culture, and is a medium generally used in plant culture. The culture medium contains 2 to 4%, preferably 3%, of sucrose serving as a nutrient, and contains 0.2 to 0.6%, preferably 0.3%, of the support, gellite. By adding sucrose as a sugar, it is possible to supply nutrients necessary for the growth of cultured plants, roots and culture seeds inside the container body without additional external nutrient supply.In addition to sucrose, other types of sugar such as glucose and fructose Can be used as Gellite is used as a support.

The culture vessel body of the present invention includes a plant, a culture root or a culture seed cultured in-flight therein, and is made of a transparent material such as transparent glass, plastic, or crystal, thereby allowing the consumer to grow the living plant culture process. It is desirable to be able to directly observe, and through this, there is an advantage that can satisfy the needs of consumers seeking change and well-being. At this time, the size of the container is preferably 1-5 cm, but is not necessarily limited thereto.

The sealing member should be a material capable of blocking the infiltration of foreign substances or bacteria into the container body and at the same time the inflow of air necessary for the growth of plants and the discharge of harmful air generated during growth. It is not limited.

Hereinafter, the present invention will be described in detail by way of examples.

However, the following examples are only for illustrating the present invention, and the content of the present invention is not limited by the following examples.

Example 1 Preparation of Accessories Including Dwarfized Plants

<1-1> Preparation of Dwarfized Plant

The young leaves of Belarus were sterilized with 1% sodium hypochlorite and 2-3 drops tween20 for 10 minutes and washed 3 to 5 times with sterile water. Surface sterilized leaves were dented on MS basal medium (Ducefa or Sigma CO. LTD.) Added with 3% sucrose and 0.3% gelite. The medium was prepared by adjusting the pH to 5.8 before autoclaving and autoclaving at 121 ° C. for 15 minutes. When leaves were incubated for 4 weeks at 25 ° C. with a photoperiod of 16 hours at 2,000 to 2,500 lux, a large amount of shoots were generated, and the shoots were passaged in medium of the same composition to obtain dwarfed shoots for 3 weeks. .

<1-2> Preparation of accessories including the plant itself

The dwarfed cultured plants prepared in Example <1-1> were dentured in a 3 cm-sized transparent culture vessel in which MS medium to which pigments, 3% sucrose and 0.5% gelite were added was injected into a sterile work bench. Accessories including the plant itself as shown in 1 were prepared.

Example 2 Preparation of Accessories Containing Cultured Roots

The roots of 200 years old wild ginseng (purchased from Manmin Food Co., Ltd.) grown in the mountains were put in 1 to 2% hypochlorous acid and 2-3 drops of twin, sterilized for about 3 to 7 minutes, and washed with sterile water. The surface sterilized wild ginseng was cut 1 mm thick in a transverse MS medium containing 1 to 2 mg / L of 2,4-D (Duchfa or sigma CO. LTD), 3% sucrose and 0.3% gelite. It was healed. The medium was adjusted to pH 5.8 before adding the gellite and autoclaved at 121 ° C. for 15 minutes. Healed wild ginseng induced callus after cancer culture at 25 ° C. for about 4-6 weeks. Subsequently, the induced callus was propagated in a medium of the same composition, followed by subculture on 1 to 2 mg / l IBA (Indole Butyric acid, Duchefa or Sigma CO. LTD) added MS basic medium and cultured for 2 to 4 weeks. Thereby forming a root muscle. The formed root muscle was grown on an MS liquid medium to which 2 mg / L IBA, 3% sucrose, 1/2 inorganic salt was added, and grown in an air-lift bioreactor.

After cutting the proliferated adductor into a size of 2-3 cm, an accessory including the culture root as shown in Figure 2 was prepared in the same manner as in Example <1-2>.

Example 3 Preparation of Accessories Containing Cultured Seeds

Seeds of carrots and anises were sterilized with 1% hypochlorous acid and 2-3 drops of twin for 10 minutes and then washed 3-5 times with sterile water. The surface sterilized carrot seeds were incubated for about 7 days, and cotyledons and hypocotyls were obtained. Anise was 1 mg / l 2,4-D, 3% of the zygotic embryos extracted directly from the seeds under anatomical microscope. MS base medium was added to the cross and 0.3% gelite. The medium was adjusted to pH 5.8 before adding the gellite and autoclaved at 121 ° C. for 15 minutes. Each of the explanted sections were cultured for 4 weeks at 25 ° C. in cancer culture to induce embryogenic callus, and the induced embryogenic callus was subcultured with liquid medium of the same composition at 2,000 to 2,500 lux. After shaking for 2 weeks at 25 rpm at 100 rpm for 16 hours with a photoperiod of 16 hours, a single cell was obtained by filtration with a 200 μm sieve. The obtained single cells were cultured in MS liquid medium without 2,4-D for 1 to 2 weeks to generate a large amount of somatic embryos, and the somatic embryos were generated by ABA (abscisic acid, Duchefa or plant growth inhibitor). Miniaturized plants were prepared by culturing for 1-2 weeks in MS liquid medium to which Sigma CO.LTD) was added to inhibit growth. In the case of the seed obtained in the open field, the surface sterilization of the seed in the same manner as above, after extracting the zygote embryo from the seed under a dissecting microscope, it can be used after incubating for 1 to 2 weeks in MS liquid medium with ABA.

An accessory comprising a cultured seed as shown in FIG. 3 was prepared in the same manner as in Example <1-2>, in which the growth-inhibited somatic embryo and conjugate embryo were prepared.

As described above, the accessory using the plant of the present invention includes a medium and a culture plant in a small transparent container, and grows it, thereby providing the effect of accessorying the plant without the hassle of supplying water or nutrients periodically. In particular, the visual effect can be improved by adding a change in color by adding a pigment, etc. Furthermore, there is an advantage of observing the change according to the growth of living in-flight culture plants and roots through transparent containers.

Claims (12)

Cultured plants were incubated for 2 to 6 weeks in a photoperiod of 12 to 16 hours at a light intensity of 2,000 to 2,500 lux using a culture medium to which sucrose and gellite were added. An accessory using a plant, characterized in that it comprises a dwarfed plant formed by subculture, a culture vessel including the same, and a sealing member for sealing the culture vessel in a sterile state. The accessory using the plant according to claim 1, wherein the culture plant is selected from the group consisting of a dwarfed plant itself, a culture root and a culture seed. 3. The accessory using a plant according to claim 2, wherein the dwarfed plant is selected from the group consisting of Valus, Rau and African Violet. The accessory using the plant according to claim 2, wherein the culture root is hairy root or irregular root obtained by tissue culture of the root of a plant selected from the group consisting of wild ginseng, ginseng, daily herb, astragalus, chichi and shiho. The accessory using the plant according to claim 2, wherein the culture seed is a seed germ or somatic embryo. According to claim 1, wherein the culture medium is accessories using plants, characterized in that the medium is selected from the group consisting of MS, B5, white (WHITE), N6, SH and Anderson (Anderson). According to claim 1, wherein the sucrose accessories using a plant, characterized in that the concentration range of 2 to 4% of the weight of the medium. According to claim 1, wherein the gellite accessories using a plant, characterized in that the concentration range of 0.2 to 0.6% of the weight of the medium. According to claim 1, wherein the culture medium is an accessory using a plant, characterized in that it further comprises food coloring. According to claim 1, wherein the culture vessel is an accessory using a plant, characterized in that selected from the group consisting of transparent glass, transparent plastic and crystal. delete The accessory using a plant according to claim 1, wherein the sealing member is a cork member.

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