KR100392515B1 - The method and the apparatus for production of transplants using microhydroponic culture system - Google Patents

Abstract

The present invention relates to a seedling production apparatus and production method of the in-flight newsletter using a microhydroponic culture system.

More specifically, micro-cutting multiple shoots of the news material, which leaves only the final rooting and purifying process through the growth point culture, leaving the final rooting and purifying process, and implanting them into the culture box by implanting them into the plant support;

The news material implanted into the culture box was supplied with oxygen and a culture medium at a temperature of 24 ± 4 ° C. and a brightness (PPF) of 110 to 350 μmol · m ⁻² · s ⁻¹ and CO _{2 of} 350 to 2000 ppm, and the upper surface of the porous wrap Culturing until the middle of the growth in a culture box (covered with),

The cultivated mid-term growth material of the medium is characterized in that it comprises the step of removing the wrap and growing at a brightness of 350μmol · m ⁻² · s ⁻¹ and shipping, compared to the conventional seedling production method by in-flight culture The labor saving effect is excellent, and it is possible to prevent the failure of the purification process and to provide high quality seedlings by providing photosynthetic environment during in-flight cultivation.

Description

The method and the apparatus for production of transplants using microhydroponic culture system}

The present invention relates to a seedling production apparatus and a method for producing small plants in-flight using plant tissue culture and hydroponic culture (Microhydroponic Culture System), which can produce high quality seedlings while attaining labor saving effect required for seedling production work. It would be.

Seed production by plant tissue culture is a very important and labor intensive and laborious task in the horticultural industry. In general, seedling production by tissue culture starts from in-flight acquisition of healthy cells or growth points from specific genetic resources desired, proceeds with the desired number of growth processes, separates them from multiple shoots into single shoots, and finishes them on rooting medium and completes the purification. A total of 345.75 people will be required. At this time, among the various processes, only 290.5 workers are given only to rooting and purifying, and most of the labor force (84.18% of the total work force) will be concentrated during this period.

In addition, news materials that have grown and rooted must be purified to environmental conditions outside of the packaging to be packaged. Tissue cultured plants, however, are supplied with over 99% humidity, high levels of nutrients, phytohormones, vitamins, and even sugar, the final metabolite of plants, to grow and absorb nutrients in the incubator. When taken out of the culture bottle as an incomplete plant that is not fully developed, adaptation, that is, it may not be purified and kills a large number of plants, may make the production of seedlings by tissue culture difficult.

Therefore, there is a need to establish a production system that can produce more economically sound blue seedlings.

The present invention has been made in view of the above matters, and an object of the present invention is a plant support suitable for proceeding rooting, growing, and purifying a news object separated from multiple shoots in a one-step. And a culture incubator.

It is another object of the present invention to provide a seedling production method capable of producing high quality seedlings by identifying the conditions and culture methods of the culture medium suitable for rooting, growing, and purifying the news material.

1 is a perspective view of a culture incubator according to the present invention.

Figure 2 is a cross-sectional view of the culture incubator according to the present invention.

Figure 3 is an illustration of the use state of the support of the culture incubator according to the present invention.

<Description of Signs of Major Parts of Drawings>

10: cultural box 11: body

12: Hole for a air exchanges 13:

13 ': aluminum mat 13 ": plug tray

14: Culture medium 15: Millipore wrap cover

16: Air meter 17: Distributor

18: notch 19: pump

20: Small plants

In order to achieve the above object, the seedling production method of the in-flight news material using the microhydroponic culture system according to the present invention, the growth of the news material through the growth stage through the growth point, leaving only the final rooting and purifying process of the Microcutting multiple shoots and implanting them into a culture box on the plant support;

The news material implanted into the culture box was supplied with oxygen and a culture medium at a temperature of 24 ± 4 ° C. and a brightness (PPF) of 110 to 350 μmol · m ⁻² · s ⁻¹ and CO _{2 of} 350 to 2000 ppm, and the upper surface of the wrap was porous. Culturing until the middle of the growth in a culture box (covered with),

The cultivated mid-term growth material of the medium is characterized in that it comprises the step of removing the wrap and growing at a brightness of 350μmol · m ⁻² · s ⁻¹ and shipping, compared to the conventional seedling production method by in-flight culture The labor saving effect is excellent, and it is possible to prevent the failure of the purification process and to provide high quality seedlings by providing photosynthetic environment during in-flight cultivation.

Hereinafter, the present invention will be described in detail.

(1) Construction of cutural box and news carrier support mat

A plurality of ventilation holes 12 are formed in one body 11 and a high light transmitting culture box 10,

A support mat 13 installed for tooth placement at a predetermined height from the bottom of the incubator 10,

A culture solution 14 filled between the bottom of the incubator 10 and the support 13;

And a microporous millipore wrap cover (15) is covered on the upper end of the incubator (10),

One side of the incubator 10 is provided with an air conditioner 16 and an air pump 19 to supply oxygen to the culture medium 14 through the distributor 17.

The support 13 floating in the culture solution 14 in the incubator was formed so that the small news object 20 could be supported and grown by forming a cross-shaped cutout 18.

In another embodiment of the present invention, the support 13 is composed of a high thermal insulation aluminum mat (13 ') or a plug tray (plug tray) (13 ").

The bottom of the incubator (inflight) of the present invention configured as described above is slightly different depending on the type of plant, but the culture medium 14 is stored at a height of about 3 cm to 6 cm from the bottom, and a support formed with a sheath in a cross shape thereon ( Run 13).

In the cross-shaped cutout portion 18 formed in the support 13, the news object 20 is sandwiched and supported so that the news object grows and the stem grows thicker. Newspapers are not disturbed at all when they are grown.

In addition, the culture solution 14 of the present invention can supply the culture solution and oxygen to the pump 19 as the news material is grown.

The amount and temperature of air in the incubator 10 is always kept constant by the air regulator 16.

Seedling production method according to the configuration of the present invention will be described.

(2) seedling production method

Plant support made of an aluminum mat (13 ') as described above by microcutting multiple shoots of the news material, which leaves only the final rooting and purifying process through the growth stage through the growth point culture. It is then implanted into one of the culture boxes of Gerbera culture (Electric Conductivity (EC) 2.0).

The news material implanted into the culture box was supplied with oxygen and a culture medium 14 at a temperature of 24 ± 4 ° C., a light intensity (PPF) of 110 to 350 μmol · m ⁻² · s ⁻¹ , and CO ₂ 350 to 2000 ppm. In order to create an environment, the upper surface is incubated in the culture box 10 covered with the porous millipore wrap cover 15 until the middle of the growth period.

In order to progress the rooting, growth, and purifying of the microcutting in-flight newsletter in a one-step process, the culture is carried out in a culture box 10 to actively root and grow the ground. By mid-term, the in vitro environment should be created, and in the later stages of growth in the above-ground and underground areas, it should be possible to create an ex-vitro environment such as the external environment for packaging transplantation.

Seedling production by conventional tissue culture is carried out aseptically in small culture bottles containing high concentrations of nutrients, plant hormones, vitamins and sugars for rooting of microcutting news materials. In the case of seedling production, the news material is designed to grow by ingesting the nutrients present in the culture bottle without active photosynthesis. Labor is needed and most of this work is concentrated on rooting and purifying.

Therefore, in the present invention, to provide conditions for active growth by photosynthesis, by removing sugar and vitamin supply, lowering the EC concentration to an appropriate level, increasing the carbon dioxide (CO ₂₎ supply and brightness, the news material is active. Environmental conditions were created to enable photosynthesis, so that when rooting and ground growth were progressed to some degree, purifying for transplantation into the pavement was carried out simultaneously.

The culture medium in the mid-growth of the growth material is gradually purified to the outside and then shipped, in order to proceed slowly in the second half of the growth process, the porous millipore wrap cover that covered the top of the culture box (culture box) to create an in-flight culture environment You can complete the purification with a simple operation that tears off a bit.

This purification process takes a period of about two days, and simply summarize the total production period is shown in Table 4 below.

Hereinafter, the present invention will be described in detail with reference to the following Examples.

Example 1 Comparison of Plant Growth by Microhydroponic Culture System ( in-ex vitro ) and Existing Tissue Culture ( in vitro )

In order to compare the growth of the plants produced by the seedling production method according to the present invention using two gerbera 508 (Tamara) and 512 (Laco) varieties, the experiments were carried out under the conditions of Table 5 below in vitro culture ( in vitro ) Table 6 shows the rooting and growth rates of the microhydroponic culture system and the microhydroponic culture system ( in-ex vitro ).

In the results of Table 6, the microhydroponic culture system of the gerbera 508, 512 both varieties of plant height, live weight, number of roots, chlorophyll concentration, leaf area was much better than in-flight culture It is shown that the method is superior in terms of economic and quality than the conventional culture method.

However, in-flight cultivation was high in root length and number of leaves. If the root length is too long, it may cause difficulty in transplantation work.

In addition, the development of the leaves was slightly less than the conventional culture method in the Microhydroponic culture system, which can be obtained in the method of the present invention by identifying the appropriate medium in the experiment of Example 3 later. there was.

Example 2 Medium Feeding Method Suitable for Plant Growth in a Microhydroponic Culture System

In order to find a culture method suitable for the growth of microcutting news materials in a microhydroponic culture system, the amount of Ebb Flood, Deep Flood Technique, and culture medium were thinned. The medium feeding method such as mDFC (modified DFC) modified by the DFT to make the oxygen supply flowing smoothly was tested by experimenting with the most economical and suitable medium for plant growth, and the results are shown in Table 7.

All culture environments and media of this experiment were carried out in the same manner as in Example 1.

When the news material was healed in the culture box, the liquid culture medium must contain a large amount of dissolved oxygen in the medium, so that the growth of the plant is possible. As shown in Fig. 2, no significant growth differences were observed in gerbera 508 and 512 varieties depending on the medium feeding method. It is thought that this does not show a big difference in growth by uniformly adjusting the air supply amount in the medium for each system.

Therefore, if the air supply in the medium can be adjusted to the optimum conditions, it was determined that the medium supply method could be freely applied according to the respective culture conditions.

Example 3 Identification of Optimal Culture Solution for News Product Growth in a Microhydroponic Culture System

In the existing tissue culture, the rooting process is mostly carried out on the solid medium containing the root growth regulator and agar added to the MS medium, and is shipped after completion of rooting. However, in the case of rooting in the plane, as mentioned above, the difficulty of aseptic work and the need for re-transplantation and purification environment for the separate purification are required. It is necessary to identify. In addition, in the experiment of Example 1, when the microhydroponic culture using the 1 / 2MS medium (microhydroponic culture), less leaf differentiation than in-flight culture, the identification of the medium that can promote leaf differentiation is urgently needed. Therefore, the medium supply method using the DFT method of the medium supply method of Example 2, 1 / 2MS medium (EC 3.0), 1 / 3MS medium (EC 2.0, 1 / 4MS medium (EC 2.0) by adjusting the concentration of the MS medium (EC 1.5) The growth of micro-cutting material was observed using the treatment and gerbera culture (EC 2.0), and the results are shown in Table 8.

As can be seen from Table 8, the 508 and 512 varieties were slightly different in growth, but the height and root length were increased when using MS medium, and the differentiation of the leaves and the concentration of chlorophyll were high in the gerbera culture. Gerbera cultures of EC 2.0 were found to promote the growth of the qualitative aspects of plant growth in microhydroponic cultures.

[Example 4] Survival test of the news object according to the news support for the microhydroponic culture system

In-flight cultures are very small (15-30 mm long, stem diameter 1.0-2.5 mm) and incubated in the same way as conventional cuttings with immature morphological development of plants such as stool cells. As it adversely affects the survival rate of plants, there is a need for a news support that is suitable for planting such news materials, promotes growth, and can be transplanted into packaging in a simple manner at the time of shipment.

Therefore, a method of putting a news object in an empty plug tray without a separate medium by culturing using a news material support made of aluminum mat according to the present invention and using a plastic pug tray. After planting with 20 days after the tooth was compared and the results are shown in Table 9.

In the results of Table 9, when the planting density, such as 162 spheres, 288 spheres wide, did not have a significant effect on plant growth, but when the leaves are covered with a narrow planting interval of more than 400 spheres, the leaves cover the upper surface of the plug tray (plug tray) The lack of air permeability of the stem between the root and the developed forearm causes the growth near the growth point, which makes the plug tray an inadequate support for the Microhydroponic culture system. have.

However, if it is healed on an aluminum mat, the aluminum mat floats on the culture solution and the underground part continuously supplies air, so the air flow in the ground part and the underground part is smooth, resulting in a very high plant growth and survival rate of 98.58%. Came out.

In addition, as the news material grows, the grass grows longer and the stem grows thicker. The support grows in accordance with the size of the plant accordingly. It became.

As described above, the microhydroponic culture system according to the present invention is incubated from the early stage to the middle stage of growth of the denture, rooting and ground growth of the single shoot separated from the multiple shoots of the propagated news material. haejugo create the conditions (in vitro), rooting and shoot growth is given by the same to the atmosphere and the condition of gradually gioe Starting somewhat advanced culture reviews (ex vitro) to proceed with the rooting, growth, purification of the post body by one-step It was found that it is an excellent method to the culture box and the carrier support according to the present invention was confirmed to provide the optimum conditions.

According to the present invention made as described above, the rooting and purifying process that requires the most labor in seedling production through tissue culture, using a culture box (cultural box) in the same place without a separate transplant operation and in vitro effects By providing conditions that can simultaneously produce seedlings, seedling production is possible with only 42% of the labor required by the existing methods, and the failure of seedling production that occurs during the purification process, which has been pointed out as a problem with the existing methods, can be prevented. By providing conditions for photosynthesis on its own from the rooting process of the city, it is possible to provide a seedling production apparatus and a production method of the in-flight news material using a microhydroponic culture system capable of producing high quality seedlings. .

Claims (8)

Microcutting mutiple shoots of the news material, which leaves only the final rooting and purifying process through the growth point cultivation, and implants them into the culture box by implanting them into the plant support; The news material implanted into the culture box was supplied with oxygen and a culture medium at a temperature of 24 ± 4 ° C. and a brightness (PPF) of 110 to 350 μmol · m ⁻² · s ⁻¹ and CO ₂ 350 to 2000 ppm, and the upper surface was porous. Culturing until the middle of the growth in a culture box covered with a wrap; Incubating the culture medium in the mid-term growth phase is removed by the wrap and grown at a light intensity of 350μmol · m ⁻² · s ⁻¹ and shipped in the cabin using a microhydroponic culture system (Microhydroponic culture system) How to produce seedlings of news objects. The method of claim 1, The rooting medium is a gerbera culture (Electric Conductivity 2.0) characterized in that the seedling production method of the in-flight news material using a microhydroponic culture system (Microhydroponic culture system). In a box-type incubator that incubates, germinates and propagates them from the cells, tissues, seeds and complete plants of the plant, A plurality of ventilation holes 12 are formed in one body 11 and a high light transmitting culture box 10, A support mat 13 installed for tooth placement at a predetermined height from the bottom of the incubator 10, A culture solution 14 filled between the bottom of the incubator 10 and the support 13; And a microporous millipore wrap cover (15) is covered on the upper end of the incubator (10), One side of the incubator 10 is provided with an air conditioner 16 and an air pump 19 is characterized in that the plant tissue is characterized in that it is configured to supply oxygen to the culture medium 14 through the distributor 17 and to maintain a constant temperature In-flight seedling production device using culture and hydroponics. The method of claim 3, The support 13 in the incubator has a cross-shaped sheath and forms a plurality of cutouts 18 so that the small news object 20 can be supported and grown using plant tissue culture and hydroponic cultivation. Inflight seedling production unit. The method of claim 3, The support 13 is an in-flight seedling production apparatus using plant tissue culture and hydroponic cultivation, characterized in that the high thermal insulation aluminum mat (13 '). The method of claim 3, The support 13 is an in-plant seedling production apparatus using plant tissue culture and hydroponic cultivation, characterized in that the plug tray (13 "). The method of claim 3, The incubator is an in-plant seedling production apparatus using plant tissue culture and hydroponic culture, characterized in that the culture box (cultural box) for the microhydroponic culture system (Microhydroponic culture system). The method of claim 3, The incubator is an in-plant seedling production apparatus using plant tissue culture and hydroponic culture, characterized in that the culture bed (cultural bed) for a microhydroponic culture system (Microhydroponic culture system).