KR20180067045A - Microponic Acclimatization System - Google Patents

Abstract

The present invention relates to a mass acclimatization facility for a tissue culture plant to resolve a problem of a limitation on industrial use. The mass acclimatization facility for a tissue culture plant comprises: a facility house (100) forming closed appearance of an acclimatization facility to provide a seedling (160) requiring acclimatization with a required cultivation space; a cultivation bed unit (110) including an acclimatization bed (111) installed in a structure of a groove (111a) of a corrugated cardboard shape in a lateral side where air can be freely circulated to be arranged in the facility house (100) to allow plug trays (113) where the seedling (160) is planted to be seated thereon to uniformly supply air to a rhizosphere of the seedling in each plug tray (113), and a support frame (112) to support the acclimatization bed (111); an air supply means including an air supply pipe (123) consisting of a hose or a pipe with a plurality of air outlets (123a) formed downward towards the groove (111a) arranged on the acclimatization bed (111) to transport and supply air into the facility house (100) from outside the facility house (100) to supply air along the groove (111a) of the acclimatization bed (111); a humidity control means (130) consisting of a coating material of a porous film with a punched portion (131) to slowly release humidity in the acclimatization facility to the outside to automatically control the humidity in the acclimatization facility by covering the facility house (100) to enclose the exterior of the facility house (100); and an environment sensor (150) to control the temperature and humidity in the acclimatization facility.

Description

[0001] Microponic Acclimatization System [0002]

[0001] The present invention relates to a mass purification facility for tissue culture plants, and more particularly, to a plant for mass proliferation (hereinafter referred to as " seedlings ") in a tissue culture using a microponic acclimatization system and a micro- The humidity condition of the greenhouse and the humidity condition of the greenhouse.

As is well known, the cell or tissue culture of a plant refers to a method of extracting or separating organs, tissues or cells of a multi-celled plant from a plant and culturing the same in a nutrient-containing cabin to produce callus, Or a mass of amorphous form often formed in a wound), or a series of manipulations that induce a single cell population or differentiate a plant or organ directly from a callus, cell, or placental tissue, and the like.

In vitro culture of plants refers to growing seeds, embryos, organs, tissue cells, and protoplasts of a plant on a nutrient medium under sterile conditions, and cultivating the same material in a packaging or greenhouse port There is a big technical difference from general cultivation.

Tissue culture of these plants began with the cultivation of a fissionable tissue of a plant that was easy to regenerate in a simple medium of the 1950s, and it has developed so that it can be regenerated in almost all tissues of all plants and plants. In addition, dealing with cells and genes to study the basic physiology of DH (Diploid-Haploid) line and plant by microbial cultivation and cultivation for breeding is becoming common.

Reproduction by tissue culture of such plants is carried out on a small scale in relatively narrow containers (Erlenmeyer flask, Petri dishes, glass bottles) and all bacteria and fungi that breed in all microorganisms and other higher plants are removed, Has the more appropriate conditions in terms of physics as well as the nutrients and plant growth regulators necessary for the growth of the tissue. It also uses the ability of the protoplasts or cells to grow independently, It has branch characteristics.

In general, plant tissue culture is carried out by dispensing artificial medium containing various nutrients into glass containers, or light-permeable glass containers made of heat-resistant polypropylene (PP), which are light and easy to handle, and are sterilized at high temperature and pressure.

However, since glass containers are heavy and fragile, they must be handled with great care and are expensive. Polypropylene (PP) is easy to handle because of its light weight, but its light transmittance is low. The use of polypropylene (PP) containers served as a factor for raising seedling production costs.

In addition, these vessels are frequently invisible in cultured plants due to the difficulty in providing more effective ventilation frequency and providing an economical photoperiod density (PPF), and the development of the guinea pig cells in the cabin and the photosynthetic And the development of other organs such as chlorophyll concentration affecting the growth of the seedlings is incomplete. Therefore, when the seedlings are ready to be packed after the cultivation, the rate of high rate of exfoliation is very high, .

The growth of plants in the cabin shows a great difference in the growth of plants depending on the amount of light transmitted through the culture bottle and the number of ventilation in the incubator as well as the brightness in the culture chamber. .

Therefore, there is a need to establish a production system capable of producing more economically sound quality seedlings.

In order to overcome such problems, Korean Patent Registration No. 0392515 (published on Mar. 23, 2003) discloses a method of producing seedling seedling using plant tissue culture and hydroponics and a production apparatus.

The method of producing seeds of in-flight microorganisms using a microhydroponic culture system disclosed in the above-mentioned Japanese Patent Publication No. 0392515 is a method for producing seeds of a so-called micro- (mutiple shoots) the micro-cutting (microcutting) to a temperature; and a post object implanted in the culture box for transplantation into culture box and a tooth shape on the plant support 24 ± 4 ℃, light intensity (PPF) 110~350μ㏖ and m ^{- 2 >} / s < ^{-1 >} and CO _{2 of} 350 to 2000 ppm, and culturing the culture medium until the middle stage of growth in a cultural box covered with porous wraps, And removing the wraps and growing and shipment at a light intensity of 350 탆 ㆍ m ^-2 ㆍ s ^-1 .

1, an in-cabbage seed production apparatus using plant tissue culture and hydroponic cultivation is a box-shaped incubator for cultivating, germinating and propagating them from plant cells, tissues, seeds and complete plants, A culture box 10 having a plurality of ventilation holes 12 formed therein and having high light transmittance; a support mat 13 provided at a predetermined height from the bottom of the incubator 10 for a microprojectile tooth; A culture liquid 14 filled between the bottom of the incubator 10 and the support 13 and a microscopic porous millipore wrap cover 15 covering the upper end of the incubator 10, An air regulator 16 and an air pump 19 are provided to supply oxygen to the culture liquid 14 through the distributor 17 and maintain the temperature constant.

In addition, the supporter 13 floating in the culture liquid 14 in the incubator forms a cross-shaped cutout 18 so that the small supporter 20 can be supported and grown.

Through the seed production method and incubator described above, it is possible to identify the culture conditions suitable for rooting, growth, and purification of the cultured object and the culture method to produce good quality seedlings.

However, the objects that have completed the propagation and rooting process must be purified to the environmental conditions of the outdoors to be packaged.

Tissue cultivated plants are plants grown by absorbing nutrients in passive cultures, supplied up to 99% moisture and high concentration of nutrients, plant hormones, vitamins and even sugar metabolites, the final metabolites of plants, When the organ is taken out of the culture bottle with incomplete and incomplete plant, the adaptation, that is, the refining of the seedlings due to the cultivation may be difficult.

Korean Patent Publication No. 0392515 (2003.3.23, Announcement: Production method and production apparatus for in-flight seedling using plant tissue culture and hydroponics)

Accordingly, the present invention is a purification system that successfully adapts a plant that has undergone mass proliferation in tissue culture to the humidity condition of the atmosphere and the humidity condition of the greenhouse, and has the problem that there is a limit in the industrial utilization due to a low purification rate per turbulence It is developed to solve.

In other words, the present invention provides a facility capable of mass-purifying a plant that has undergone mass propagation in a tissue culture using a microponic acclimatization system so that it can be successfully adapted to atmospheric humidity conditions and greenhouse humidity conditions The purpose is to do that.

To this end, the object of the present invention is to provide a bed structure, an air supply means, and a humidity control means in a purifying facility in providing the structure of a microphonic enrichment mass production system.

Namely, it provides a temperature and humidity control type structure for mass seedling cultivation of young plant seedlings, a vinyl compartment covered with a breathable film (a film material of a porous film having tread) for humidity drop through natural volatilization, An air supply pipe for air circulation in the root portion (root) of a regular tray (plug tray or seedling box), a hollow structure of a lower groove structure for spreading the air discharged from the air supply pipe to the lower portion of the tray, And a variable diaphragm capable of dividing one line of the cultivation system as needed.

To achieve the above object, according to the present invention, there is provided a method of manufacturing a plant, comprising: a facility house that forms a closed outer appearance of a purification facility to provide a cultivation space necessary for seedlings requiring purification; and a plug tray disposed in the facility house, And a supporting frame for supporting the purging bed, wherein the purging bed is provided with a corrugated cardboard-like hollow structure on the transverse side in which the air circulation can be freely provided so as to uniformly supply air to the root- And a hose or pipe having a plurality of air discharge openings formed in a downward direction in the direction of the groove to be poured on the purifying bed so as to supply air along the groove of the purifying bed by feeding air from the outside of the facility house to the inside, An air supply means including an air supply means A humidity adjusting means composed of a film material of a porous film having a trough in which moisture in the purification facility is slowly discharged to the outside so that the humidity in the purification facility can be automatically controlled by covering the inside of the purification facility with temperature and humidity The present invention is characterized by providing a mass purification facility of a tissue culture plant including an environmental sensor provided for regulation.

According to the present invention, the inside of the facility house may further include a screen for partitioning the area so as to be constituted by a variable compartment in which the seedling growing area is divided for each growth period of the seedling growing plant, and a screen motor for controlling the operation of the screen. .

According to the present invention, it is preferable that the air supply means further includes a constant temperature chamber having a heat exchanger capable of regulating the air supplied into the purifying facility to a predetermined temperature and maintaining the supplied air at a required temperature.

According to the present invention, the air supply pipe may be connected to a moisture controller capable of humidifying and dehumidifying.

According to the present invention, it is preferable to further include water supply means having a water supply pipe capable of supplying and spraying water into the refinement facility.

According to the present invention, in a tissue culture using a microponic acclimatization system, a plant that has undergone mass propagation can be successfully adapted to atmospheric humidity conditions and greenhouse humidity conditions, and can be mass- There is an effect that can be.

Further, according to the present invention, it is possible to provide a seedling growing and purifying system capable of environmental control by zone using a variable compartment for producing a small variety of products.

Brief Description of the Drawings Fig. 1 is a schematic perspective view showing a seedling production apparatus using plant tissue culture and hydroponic cultivation disclosed in Korean Patent Registration No. 0392515 according to the prior art.
FIG. 2 is a schematic view showing a schematic overall structure of a mass purification facility of a tissue culture plant according to the present invention. FIG.
FIG. 3 is a schematic diagram showing a more detailed schematic structure of the facility house of the mass purification facilities of the tissue culture plant according to the present invention. FIG.
FIG. 4 is a schematic view showing a configuration of a cultivation bed part of a mass purification facility of a tissue culture plant according to the present invention. FIG.
FIG. 5 is a schematic view showing the arrangement relationship between a purifying bed and an air supply pipe of a cultivation bed unit according to the present invention; FIG.
Figure 6 schematically shows the construction of an air supply means comprising a constant temperature chamber and a moisture regulator according to the invention;
FIG. 7 is a schematic view showing the structure of a coating film of a porous film which is a humidity controlling means according to the present invention. FIG.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

In order to explain the preferred embodiments of the present invention, the thicknesses of the lines and the sizes of the components shown in the attached drawings may be exaggerated or omitted for clarity and convenience of description, Terms to be given are terms defined in consideration of the functions of the present invention, which may vary depending on the intention or custom of the user, the operator.

As shown in FIGS. 2 and 3, the mass purification facility (or purification system) of the tissue cultured plant according to the present invention can be used for producing a small variety of products by using a variable compartment, System.

That is, the mass purification facility (or purification system) of the tissue culture plant according to the present invention includes a facility house 100, a cultivation bed unit 110, an air supply unit 120, a humidity control unit 130, And a screen means (140).

Here, the facility house 100 is a seedlings growing space made up of a framework frame such as a vinyl house that forms a closed outer appearance of a purification facility and provides a space required for the seedlings 160 requiring purification. In order to carry out the process of mass producing the seedlings 160, diaphragm screen means 140 for dividing the seedlings purifying system, which requires control of the environment according to the age of the seedlings, ).

For example, the inside of the facility house 100 can be constituted by a diaphragm screen unit 140 and a variable compartment in which a seedling growing area is divided for each growing plant. The first growing area 101, the second growing area 102) and a 3-stage growth zone 103, so that the purification environment can be controlled according to the age of the tomb.

As described above, the diaphragm screen means 130 can constitute a variable compartment by dividing the zones according to the growth stages of the seedlings 160, and a roll screen or a shutter screen can be used.

The roll screen or shutter screen of the diaphragm screen means 140 is constituted by a screen 141 made of a flexible material and is controlled by the control motor 142 in each compartment (or diaphragm, that is, 2-stage growth zone 102, 3-stage growth zone 103).

When the compartment is partitioned in the purification facility as described above, it is possible to separate the compartments (i.e., the first, second, Supply, etc.) can be controlled so that a control target point can be set for each zone.

4 and 5, the cultivating bed unit 110 can seat the plug tray 113 on which the seedlings 160 are planted and uniformly supply air to each of the plug trays 113 A purifying bed 111 installed in a corrugated structure in a transverse direction so as to allow free air circulation and a supporting frame 112 for supporting the purifying bed 111. [

At this time, the purification bed 111 has a structure in which recesses 111a (or valleys) are formed in the form of corrugated cardboard using a material such as transparent PVC or PC, and the purification bed 111 is, for example, It is preferable that the plug trays 113, which are standardized on the market and commercially available, are arranged in two rows. However, the arrangement of the plug trays 113 is not limited to two rows.

The support frame 112 is a normal frame structure that supports and supports the purifying bed 111 at a predetermined height from the ground.

The air supply unit 120 continuously supplies air to adjust the humidity in the humidification facility of 95% humidity.

2 and 6, the air supply unit 120 is installed in the root portion of the seedlings 160 in the respective plug trays 113 mounted on the cultivation bed portion 110, A gas supply pipe 123 for transferring and supplying air (or gas) which has been thermally treated through the constant temperature chamber 121; And a fan motor 124 that provides power to send air (or gas) to the air supply pipe 123.

When the seedlings are designed to perform the purifying process for each growth period by dividing into the first growth zone 101, the second growth zone 102 and the third growth zone 103 in the purification facility, the air supply means 120) are provided in separate independent lines for each growth period.

2 and 3, only one air supply means 120 is shown and one air supply pipe 101 is connected to the first growth zone 101, the second generation zone 102 and the third generation zone 103 123, and arranged in a straight line, but this is only schematically shown due to difficulties in the drawings.

For example, the air supply means 120 and the air supply pipe 123 constituting the air supply means 120 are divided into a first growth stage 101, a second stage growth stage 102, a third stage growth stage 103, It is preferable to arrange them in separate independent lines, and it is reasonable to understand that they have such an arrangement structure.

At this time, the air supply pipe 123 composed of separate independent lines for each growth cycle is a hose or pipe having a plurality of air discharge openings 123a formed at regular intervals, and air (or gas) is supplied from the constant temperature chamber 121 And uniformly supplies the purified water to the refining bed 111 of the cultivation bed part 110.

For example, the air supply pipe 123 may be a vinyl tube type hose having a size of about 6 cm, and a plurality of air discharge openings 123a having a uniform size and uniform spacing downwardly of the vinyl tube type hose may be formed .

The air discharge pipe 123a of the air supply pipe 123 is disposed in the recess 111a of the purifying bed 111. The air discharge pipe 123a of the air supply pipe 123 is disposed on the side of the upper surface of the purifying bed 111, So that the air injected into the groove 111a of the refinement bed 111 flows naturally by spraying the temperature-controlled air through the air outlet 123a of the air supply pipe 123, Air routed to the root of the seedlings 160 planted in the plug tray 113 disposed on the purification bed 111 flows uniformly into the root of the seedlings 160 as air supplied to the seedling 111 flows naturally along the grooves 111a. (I.e., oxygen) can be supplied.

The air supply pipe 123 is connected to a moisture controller 125 capable of humidifying and dehumidifying the air to supply air (or gas) through the air supply pipe 123, (Or gas) in a dehumidified and dried state is supplied to the purifying bed 111 so that the humidity in the purifying facility can be effectively controlled.

The constant temperature chamber 121 of the air supply means 120 is provided with an air suction pipe 126 for sucking and supplying air and a gas suction pipe 127 for sucking a target gas such as carbon dioxide, Way valve 128 is provided between the gas suction pipe 126 and the gas suction pipe 127 so that the opening and closing directions of the pipes can be controlled. When the gas is supplied to the air supply pipe 123, it is time to provide carbon dioxide (CO ₂ ) necessary for the late growth period of the seedlings 160.

The constant temperature chamber 121 is provided to provide air or water adjusted to a constant temperature in order to effectively control the humidity in the process of supplying air into the purification facility, So that the supply temperature of air or water can be maintained at a required temperature and supplied through the supply pipe 122.

The constant temperature chamber 121 may be divided into a water supply unit and an air supply unit.

The humidity adjusting means 130 can automatically adjust the humidity in the purifying facility of 95% humidity so that the moisture in the purifying facility is slowly discharged to the outside for a predetermined period to be used for the purpose of lowering the natural humidity.

That is, the humidity control means 130 uses a film material of a film material having fine troughs 131 so that moisture can be discharged to the outside, and the film material of the film material 100 So that the facility house 100 and the outside can be shut off.

By covering the facility house 100 with the coating material made of the air-permeable porous film, the air is gradually emitted (or vaporized) so that the humidity can be automatically controlled through the air supply from the seedlings 160 during planting.

As shown in FIG. 7, a breathable porous film is used for discharging the moisture in the inside of the purification facility to the outside. For example, a pore size of the tread 131a, which alternates at a predetermined interval, Is in the range of 0.01 to 10 mm, the perforation interval is 2 to 500 times the perforation size, and the thickness of the perforated film is preferably 0.03 to 5 mm.

In order to satisfy the humidity condition of 99% in the purification facility, it is also possible to include in a microphonic system a plant material (Peat moss, Spagmum moss, Perite, etc.) have.

In addition, in order to control the temperature and humidity, which are key factors in creating the environment for purification of the seedling (160) process, an environmental sensor (150) is provided in each zone.

By providing the environmental sensor 150, it is possible to provide the constant temperature chamber 121 set for each seedling 160 period so that the raw water or the introduced air is supplied to the water or air Can be supplied according to the measured value of the environmental sensor 150 and the target value of the user.

In the meantime, it is preferable to provide water supply means 170 for supplying water in the mass purification facility according to the present invention.

The water supply means 170 includes a purified water tank 174 that can purify water, a pump 175 that supplies power to supply and supply water, a water supply pipe 171 that transfers water into the purification facility, A water supply control valve 172 provided in the water supply pipe 171, and a pressure type nozzle unit 173.

The water supply pipe 171 may be divided into a water supply pipe for the water spray nozzle and a water supply pipe for the mist, if necessary, and may have a respective constant temperature chamber 121.

The pressure-type nozzle unit 173 attached to the water supply pipe 173 can be replaced with a nozzle unit having a detachable accessory such as a water-spraying type, a mist-type or the like, if necessary.

According to the mass-purifying facility for cultivating a tissue culture according to the present invention having the above-described structure, in the seedling purifying process for mass production, the seedling growing and purifying system, in which control of the environment according to the age of the seedlings 160 is required, And is partitioned by the diaphragm screen means 140. [

The water or air is supplied through the air supply means 120 and the water supply means 170 in accordance with the measured value of the environmental sensor 150 and the user's desired value, (Or volatilization) naturally externally conducts a purification process that successfully adapts to the atmospheric humidity conditions and the greenhouse humidity conditions.

At this time, the young seedlings 160 of the first-stage growth area 101 are purified by the above-mentioned predetermined period, and then transferred to the second-stage growth area 102 to carry out the purifying process according to the second- In addition, a young seedling (160) to be prepared for the first seedling growth period is introduced into the first seedling growing area (101) to undergo the seedling growing and purifying step according to the first seedling growing period.

In the second stage growth zone 102, the seedlings 160, which have been subjected to the purifying process in accordance with the second stage growth period, are sent to the third stage growth zone 103 so as to undergo the purification process corresponding to the third stage growth period, It is possible to systemize the purification process step by step according to each seedling growth cycle and proceed the purification process in a large amount.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but, on the contrary, It will be understood by those skilled in the art that various modifications may be made and equivalents may be resorted to without departing from the scope of the appended claims.

100: Facilities House 101: 1 planting area
102: 2 growth zone 103: 3 growth zone
110: Cultivation bed part 111: Purification bed
112: support frame 113: plug tray
120: air supply means 121: constant temperature chamber
122: heat exchanger 123: air supply pipe
123a: air outlet 124: fan motor
125: Moisture regulator 126: Air intake pipe
127: gas suction pipe 128: three-way valve
130: Humidity adjusting means 131:
140: diaphragm screen means 141: screen
142: Control motor 150: Environmental sensor
160: seedling 170: water supply means
171: water supply pipe 172: water supply control valve
173: Pressure type nozzle unit 174: Water tank
175: Pump

Claims (5)

A facility house 100 for forming a closed appearance of the purification facility and providing a cultivation space necessary for the seedlings 160 requiring purification,
The air is circulated freely so that air can be uniformly supplied to the root portions of the seedlings 160 in the respective plug trays 113 by placing the plug trays 113 disposed in the facility house 100 and planted with the seedlings 160 A growth bed portion 110 composed of a purification bed 111 installed on the transverse side in a corrugated cardboard shape having a groove 111a structure and a support frame 112 supporting the purification bed 111,
A plurality of downwardly directed grooves 111a are provided on the purifying bed 111 so as to supply air along the grooves 111a of the purifying bed 111 by feeding air to the inside from the outside of the facility house 100, An air supply pipe 110 including an air supply pipe 123 formed of a hose or pipe provided with an air discharge port 123a of the air supply port 123a,
A coating material of a porous film having a tread 131 in which moisture in the purification facility is slowly released to the outside so that the humidity in the purification facility can be automatically controlled by covering the outside of the facility house 100, A humidity adjusting means 130,
And an environmental sensor (150) provided for controlling temperature and humidity in the purification facility.
The method according to claim 1,
A screen 141 for dividing the inside of the facility house 100 so that the inside of the facility house 100 can be constituted by a variable compartment in which a seedling growing area is divided for each growth period of the planting house 160 and a control motor 142 for controlling the operation thereof. (140). ≪ Desc / Clms Page number 20 >
3. The method according to claim 1 or 2,
Wherein the air supply means (120) further comprises a constant temperature chamber (121) having a heat exchanger (122) capable of regulating the air supplied into the purifying facility to a predetermined temperature and maintaining and supplying the air at a required temperature Mass purification facilities of cultivated plants.
3. The method according to claim 1 or 2,
Characterized in that the air supply pipe (123) is connected to a moisture controller (125) capable of humidifying and dehumidifying.
3. The method according to claim 1 or 2,
Further comprising a water supply means (170) having a water supply pipe (171) capable of supplying and spraying water into the refinement facility.

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