KR100367286B1 - Method for Extracting the Extra ct of Detoxicated Rhus Verniciflua - Google Patents

Abstract

The present invention relates to a method for extracting poisonous lacquer extract, and more specifically, a first toxic removal process for hot-air drying the sap or bark collected from the lacquer tree, and adding a solvent to the dried lacquer sap or bark and heat treatment. By removing the toxicity and at the same time to extract the lacquer extract and the extraction method of the lacquer extract consisting of a concentrated process of filtering and concentrating the extracted lacquer extract after filtering.

According to the extraction method of the present invention, the extract of the lacquer tree from which the toxicity has been removed can be obtained, and the extract can be used as a raw material for food raw materials and pharmaceuticals such as functional foods, health supplements, and food additives.

Description

Method for Extracting the Extra ct of Detoxicated Rhus Verniciflua}

The present invention relates to a method of extracting the poisonous lacquer extract, and more specifically, a first toxic removal process of hot air drying the sap or bark taken from the lacquer tree, and adding a solvent to the dried lacquer sap or bark and heat treatment. By removing the toxicity and at the same time to extract the lacquer extract and the extraction method of the lacquer extract consisting of a concentrated process of filtering and concentrating the extracted lacquer extract and filtered.

Conventional lacquer edible young shoots as herbs in early spring, dried shells, etc., put together with chicken, edible white porcelain, or was used as a herbal medicine. Lacquer sap has been used as a raw material for paints for a long time, but due to its toxicity, lacquer workers have been urgently required to remove the poison of lacquer.

From the old sumac (Rhus verniciflua) has been widely cultivated in the East, especially in Korea, Japan and China have been collected sap that is taking a cut sumac bark produced here, mainly used in lacquer (lacquer). In particular, the Najeon lacquer ware of Korea cannot be compared with the lacquer and enamel currently used, and is recognized worldwide for its excellent quality. In folk medicine, lacquer medicinal herbs and foods have been used only in secret, but their use has been limited due to toxic (allergic) reactions.

Lacquered chickens and lacquer shoots that have been eaten since ancient times have been difficult to commercialize due to the fear of allergies (usually "raised"), and many damages have been reported. In particular, dermatology hospitals are determined to be contraindicated. On the other hand, there are many studies on the treatment of allergic reactions caused by the ivy of the sumac in the foreign countries, but few countries eat for food as in the case of Korea.

The main component of lacquer sap (lacquer) is urushiol, and its main use is pale yellow liquid, which is used for lacquerware, and is known as a nonpolar substance dissolved in alcohol, ether, and chloroform. The chemical structure is an unsaturated compound containing side chain R groups containing 15 to 17 carbon atoms double bonds, mixed in 5 mixtures (Merck index 9697), and the main pharmacological action of lacquer is anticancer, antioxidant, hangover, etc. (Korea Forest Service, Forestry Breeding Research Institute Nacheon-gu Research Team, April 22, 1997, Food Science Society, vol 31, 238-245), gastrointestinal disease, heart disease, arthritis, hypertension, diabetes, stroke, arthritis, chronic fatigue, etc. , Herbology).

Despite these various pharmacological actions, the cause that has not yet been commercialized is known because of toxic effects (allergies) on the human body. Therefore, in order to actively use the pharmacological effects of lacquer trees, a method of eliminating poisoning of lacquer trees is urgently needed. In addition, the material extracted from the lacquer tree has excellent anti-oxidation and anti-cancer properties, so if the compound is separated, the new application range will be expanded.

The present inventors have conducted research to solve this problem, and firstly removes the poison by drying the sap or bark collected from the lacquer tree, and adding the solvent to the dried lacquer sap or the bark and heat treatment to remove the toxicity. At the same time, it was found that the antioxidant and anticancer components, which are natural bioactive substances, can be extracted from the lacquer extract, thereby completing the present invention.

Accordingly, it is an object of the present invention to provide a novel extraction method of the sumac extract, which has been removed toxicity.

1 is a process chart of the extraction method according to the present invention.

Figure 2 is a photograph showing non-toxicity when applying the extract according to the invention on the skin.

Extraction method of the extract of the poisoned lacquer tree according to the present invention is the first toxicity removal process for hot-air drying the sap or bark taken from the lacquer tree, and adding the solvent to the dried lacquer sap or bark and heat treatment to remove the toxicity It consists of a second removal and toxicity process of extracting the lacquer extract and a concentration process of filtering and concentrating the extracted lacquer extract.

Hereinafter, the extraction method according to the present invention will be described in detail for each process.

1st process (1st detoxification process)

Lacquer sap (lacquer) was collected from the lacquer tree over 5-6 years old after planting and dissolved in organic solvents such as ethanol, methanol, acetone, chloroform, and filtered. Drying at temperatures of 400 ° C. for 3 to 24 hours primarily removes toxicity.

Meanwhile, lacquer bark and wood are first dried in a hot air dryer at 100-400 ° C. for 3 to 24 hours to remove toxicity.

2nd process (2nd detoxification and extraction process)

Lacquer sap is dissolved by adding 1-10% (v / w) of organic solvents such as ethanol, methanol, acetone, and chloroform in the dry state.The lacquer is heat-treated with the raw material (lacquer bark) in a pressure-resistant container filled with water. Put it in a 0.5-2.5 ㎏ / ㎠ pressure, heat treatment for 30 minutes-24 hours at a temperature of 105-200 ℃ to remove the toxic and secondly extract the poisonous lacquer extract at the same time.

3rd process (concentration process)

The extracted sumac extract is filtered and then evaporated and dried using a vaccum evaporator until the solvent (water or organic solvent) is completely removed at a temperature of 50-90 ° C.

4th process (dilution process)

Evaporated and dried lacquer extract is diluted to 50 to 2,000 times by weight using water or an organic solvent. For long term storage, add ethanol so that the concentration is 10% (v / w) and store.

5th process (separation process)

The diluted sample is evaporated, concentrated and diluted by adding 3 times distilled water, and then separated from the anti-oxidizing material and the anticancer substance by using an ODS column which is an anion, cation exchange resin, silica, phenyl, cyan and reverse phase columns.

The separation method uses water and a solvent (acetnitrile, methanol, chloroform) to separate and purify the bioactive material under the separation conditions of the solvent concentration between 0% and 100% at a rate of 1-5ml per minute.

Hereinafter, the effect of the sumac extract extracted by the method according to the present invention through the following test example in detail.

Test Example 1 Toxicity Test of Lacquer

As a control, lactose sap, lacquer tree extract and lactose extract according to the present invention, which were not toxic to lacquer, were used for 40 adult men and women (3 males and 37 females) over 20 years old. The reaction test of skin was performed.

Lacquer sap and extract according to the present invention was diluted 10 times with a solvent (ethanol, methanol, chloroform), lacquer sap (lacquer) was used as a stock solution, extracts that do not remove the toxicity using the stock solution, the back of the hand soft skin, Directly applied to the underarm and armpit, the change in lacquer toxicity of the skin was observed and shown in Table 1.

Table 1. Reaction test on the skin of lacquer extract

Examiner Control (no treatment) Treatment tool (poison removal) Remarks 40 people in total 39 persons 1 person Toxic reaction 97.5% 37 women 37 people 0 people Toxicity 100% 3 men 2 people 1 person Toxic reaction 66.7%

From the above results, when the test was conducted on 40 lactoxin testers, 97.5% showed no toxic response in the untreated group, while only 2.5% showed toxic reactions in the treated group. It can be seen that.

Test Example 2 Antioxidant Reaction of Lacquer Tree

Antioxidant activity was measured by using a spectrophotometer (DPPH radical scavenging ass ay; Blois MS, Antioxidant determination by th use a stable free radical, Nature 26 , 1199-1200, 1958).

20 μl of extract extracted from lacquer was reacted for 20 minutes at 37 ° C. and the antioxidant power was measured by DPPH method using the difference in absorbance. The control group showed 1.2 and the lacquer extract showed 0.1, showing a difference in absorbance of 1.1.

DPPH (1,1-dihenyl-2-picryl hydrazyl) free radicals have absorbance at 515 nm, which is reduced when radical scavenging occurs due to antioxidants. Radical scavenging activity was evaluated. As a comparative measure of antioxidant activity (%), when the vitamin C was 100, lacquer extract was 50 and green tea was 40.

According to the extraction method of the present invention, the extract of the lacquer tree from which the toxicity has been removed can be obtained, and the extract can be used as a raw material for food raw materials and pharmaceuticals such as functional foods, health supplements, and food additives.

On the other hand, the extract by the extraction method of the present invention is not a chemical compound, but a material extracted from natural materials will be able to further expand the application range.

Claims (5)

A first detoxification step of hot air drying the sap or bark collected from the lacquer tree; Adding a solvent to the dried lacquer sap or shell and heat treatment to remove the toxicity and at the same time to extract the lacquer extract; Extraction method of extracting poisonous lacquer tree, characterized in that consisting of a concentrated process of filtering and then concentrated the extracted lacquer extract. The method of claim 1, wherein the first detoxification process is a method of extracting detoxified sumac extract, characterized in that the hot air is dried for 3 to 24 hours at 100 to 400 ° C. The process of claim 1, wherein the second detoxification and extraction process is carried out at a pressure of 0.5-2.5 kg / cm ² , at a temperature of 105-200 ° C., to add 1-10% (v / w) of the solvent. For hours Extraction method of the sumac extract, characterized in that the poison is removed. The method of extracting poisonous lacquer extract according to claim 1 or 3, wherein the solvent is at least one selected from the group consisting of water or an organic solvent group consisting of ethanol, methanol, acetone or chloroform. The method of extracting poisonous lacquer extract according to claim 1, wherein the concentration step is concentrated by evaporating and drying the lacquer extract using a reduced pressure condenser at a temperature of 50-90 ° C until the solvent is completely gone. .