JPWO2021209564A5 - - Google Patents
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- JPWO2021209564A5 JPWO2021209564A5 JP2022562976A JP2022562976A JPWO2021209564A5 JP WO2021209564 A5 JPWO2021209564 A5 JP WO2021209564A5 JP 2022562976 A JP2022562976 A JP 2022562976A JP 2022562976 A JP2022562976 A JP 2022562976A JP WO2021209564 A5 JPWO2021209564 A5 JP WO2021209564A5
- Authority
- JP
- Japan
- Prior art keywords
- biological sample
- nucleic acid
- acetonitrile
- virus
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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- 238000000034 method Methods 0.000 claims description 99
- 239000012472 biological sample Substances 0.000 claims description 98
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 claims description 78
- 108020004707 nucleic acids Proteins 0.000 claims description 70
- 102000039446 nucleic acids Human genes 0.000 claims description 70
- 150000007523 nucleic acids Chemical class 0.000 claims description 70
- 239000003960 organic solvent Substances 0.000 claims description 45
- 239000000523 sample Substances 0.000 claims description 31
- 241000700605 Viruses Species 0.000 claims description 30
- 239000002245 particle Substances 0.000 claims description 24
- 230000003321 amplification Effects 0.000 claims description 20
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 20
- 239000003463 adsorbent Substances 0.000 claims description 19
- 239000007788 liquid Substances 0.000 claims description 18
- 239000003153 chemical reaction reagent Substances 0.000 claims description 12
- 241001678559 COVID-19 virus Species 0.000 claims description 11
- 102000004190 Enzymes Human genes 0.000 claims description 11
- 108090000790 Enzymes Proteins 0.000 claims description 11
- 238000001514 detection method Methods 0.000 claims description 10
- 102000016911 Deoxyribonucleases Human genes 0.000 claims description 8
- 108010053770 Deoxyribonucleases Proteins 0.000 claims description 8
- 230000015556 catabolic process Effects 0.000 claims description 8
- 238000006731 degradation reaction Methods 0.000 claims description 8
- 230000000415 inactivating effect Effects 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 8
- 102000006382 Ribonucleases Human genes 0.000 claims description 7
- 108010083644 Ribonucleases Proteins 0.000 claims description 7
- 108020000999 Viral RNA Proteins 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 230000029058 respiratory gaseous exchange Effects 0.000 claims description 7
- 239000002904 solvent Substances 0.000 claims description 7
- 241000701022 Cytomegalovirus Species 0.000 claims description 6
- 241000701085 Human alphaherpesvirus 3 Species 0.000 claims description 6
- 238000007865 diluting Methods 0.000 claims description 6
- 108020005202 Viral DNA Proteins 0.000 claims description 5
- 238000007664 blowing Methods 0.000 claims description 5
- 230000001681 protective effect Effects 0.000 claims description 5
- 239000002299 complementary DNA Substances 0.000 claims description 4
- 238000009833 condensation Methods 0.000 claims description 4
- 230000005494 condensation Effects 0.000 claims description 4
- 238000003757 reverse transcription PCR Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 241000711573 Coronaviridae Species 0.000 claims description 3
- 108020004414 DNA Proteins 0.000 claims description 3
- 241000701044 Human gammaherpesvirus 4 Species 0.000 claims description 3
- 241000829111 Human polyomavirus 1 Species 0.000 claims description 3
- 102100034343 Integrase Human genes 0.000 claims description 3
- 201000005505 Measles Diseases 0.000 claims description 3
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 3
- 241000702670 Rotavirus Species 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- 238000004090 dissolution Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 239000002594 sorbent Substances 0.000 claims description 3
- 241000712461 unidentified influenza virus Species 0.000 claims description 3
- RLLPVAHGXHCWKJ-IEBWSBKVSA-N (3-phenoxyphenyl)methyl (1s,3s)-3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropane-1-carboxylate Chemical compound CC1(C)[C@H](C=C(Cl)Cl)[C@@H]1C(=O)OCC1=CC=CC(OC=2C=CC=CC=2)=C1 RLLPVAHGXHCWKJ-IEBWSBKVSA-N 0.000 claims description 2
- 238000011529 RT qPCR Methods 0.000 claims description 2
- 230000003213 activating effect Effects 0.000 claims description 2
- 238000004891 communication Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 238000012545 processing Methods 0.000 claims description 2
- 241000700584 Simplexvirus Species 0.000 claims 1
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 12
- 239000002609 medium Substances 0.000 description 12
- 210000004027 cell Anatomy 0.000 description 11
- 206010022000 influenza Diseases 0.000 description 6
- 208000030507 AIDS Diseases 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 241000725303 Human immunodeficiency virus Species 0.000 description 4
- 210000003608 fece Anatomy 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 239000000427 antigen Substances 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 210000003296 saliva Anatomy 0.000 description 3
- 206010059313 Anogenital warts Diseases 0.000 description 2
- 208000025721 COVID-19 Diseases 0.000 description 2
- 201000006082 Chickenpox Diseases 0.000 description 2
- 208000000907 Condylomata Acuminata Diseases 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 208000004898 Herpes Labialis Diseases 0.000 description 2
- 208000009889 Herpes Simplex Diseases 0.000 description 2
- 241000701806 Human papillomavirus Species 0.000 description 2
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 206010067152 Oral herpes Diseases 0.000 description 2
- 206010036790 Productive cough Diseases 0.000 description 2
- 241000725643 Respiratory syncytial virus Species 0.000 description 2
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 description 2
- 206010046980 Varicella Diseases 0.000 description 2
- 208000025009 anogenital human papillomavirus infection Diseases 0.000 description 2
- 201000004201 anogenital venereal wart Diseases 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 208000006454 hepatitis Diseases 0.000 description 2
- 231100000283 hepatitis Toxicity 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 210000003802 sputum Anatomy 0.000 description 2
- 208000024794 sputum Diseases 0.000 description 2
- 208000011580 syndromic disease Diseases 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 230000003612 virological effect Effects 0.000 description 2
- 229940096437 Protein S Drugs 0.000 description 1
- 101710198474 Spike protein Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000000234 capsid Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- -1 cell wall Chemical class 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000779 depleting effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000007373 indentation Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 239000006163 transport media Substances 0.000 description 1
Applications Claiming Priority (17)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202063010455P | 2020-04-15 | 2020-04-15 | |
| US63/010,455 | 2020-04-15 | ||
| US202063042552P | 2020-06-22 | 2020-06-22 | |
| US63/042,552 | 2020-06-22 | ||
| US202063049626P | 2020-07-08 | 2020-07-08 | |
| US63/049,626 | 2020-07-08 | ||
| US202063052269P | 2020-07-15 | 2020-07-15 | |
| US63/052,269 | 2020-07-15 | ||
| US202063073447P | 2020-09-01 | 2020-09-01 | |
| US63/073,447 | 2020-09-01 | ||
| US202063124048P | 2020-12-10 | 2020-12-10 | |
| US63/124,048 | 2020-12-10 | ||
| US202163163032P | 2021-03-18 | 2021-03-18 | |
| US63/163,032 | 2021-03-18 | ||
| US202163164520P | 2021-03-22 | 2021-03-22 | |
| US63/164,520 | 2021-03-22 | ||
| PCT/EP2021/059815 WO2021209564A2 (en) | 2020-04-15 | 2021-04-15 | Methods, devices and kits for preparing nucleic acid samples for storage and analysis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2023522054A JP2023522054A (ja) | 2023-05-26 |
| JPWO2021209564A5 true JPWO2021209564A5 (de) | 2024-04-01 |
Family
ID=75581514
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022562976A Pending JP2023522054A (ja) | 2020-04-15 | 2021-04-15 | 保存および分析のための核酸試料を調製する方法、装置、およびキット |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20210355525A1 (de) |
| EP (1) | EP4136226A2 (de) |
| JP (1) | JP2023522054A (de) |
| GB (1) | GB2595572A (de) |
| WO (1) | WO2021209564A2 (de) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2609476A (en) * | 2021-08-04 | 2023-02-08 | Laing Jason | Vapour sample collection device |
| US20230091650A1 (en) | 2021-09-20 | 2023-03-23 | Vosbio, Inc. | Devices, Methods and Kits for Biological Sample Capture and Processing |
| CN114107571A (zh) * | 2022-01-18 | 2022-03-01 | 复旦大学附属中山医院 | 用于bk病毒lamp检测的试剂盒 |
| CN115553286A (zh) * | 2022-09-23 | 2023-01-03 | 深圳微辰生命科技有限公司 | 一种粪便保存液、保存粪便的方法及乙腈在制备粪便保存液中的应用 |
| US11805995B1 (en) * | 2022-11-22 | 2023-11-07 | King Faisal University | Saliva collection kit |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7601491B2 (en) * | 2003-02-06 | 2009-10-13 | Becton, Dickinson And Company | Pretreatment method for extraction of nucleic acid from biological samples and kits therefor |
| US8080645B2 (en) * | 2007-10-01 | 2011-12-20 | Longhorn Vaccines & Diagnostics Llc | Biological specimen collection/transport compositions and methods |
| US9683256B2 (en) * | 2007-10-01 | 2017-06-20 | Longhorn Vaccines And Diagnostics, Llc | Biological specimen collection and transport system |
| US11041216B2 (en) * | 2007-10-01 | 2021-06-22 | Longhorn Vaccines And Diagnostics, Llc | Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples |
| GB2522471A (en) * | 2014-01-27 | 2015-07-29 | Uwe Warnken | Compositions for cells lysis and uses thereof |
| US9528105B2 (en) * | 2014-09-04 | 2016-12-27 | Techlab, Inc. | Nucleic acid extraction using organic solvents to remove inhibitors |
| WO2018126119A1 (en) * | 2016-12-30 | 2018-07-05 | Lee Luke P | Aerosol capture and processing device |
| WO2018198682A1 (ja) * | 2017-04-26 | 2018-11-01 | 東洋紡株式会社 | ウイルスの検査方法およびウイルスの検査用キット |
-
2021
- 2021-04-15 US US17/231,234 patent/US20210355525A1/en active Pending
- 2021-04-15 WO PCT/EP2021/059815 patent/WO2021209564A2/en unknown
- 2021-04-15 EP EP21719891.0A patent/EP4136226A2/de active Pending
- 2021-04-15 GB GB2105390.5A patent/GB2595572A/en active Pending
- 2021-04-15 JP JP2022562976A patent/JP2023522054A/ja active Pending
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