JPWO2021100829A5 - - Google Patents
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- Publication number
- JPWO2021100829A5 JPWO2021100829A5 JP2021558457A JP2021558457A JPWO2021100829A5 JP WO2021100829 A5 JPWO2021100829 A5 JP WO2021100829A5 JP 2021558457 A JP2021558457 A JP 2021558457A JP 2021558457 A JP2021558457 A JP 2021558457A JP WO2021100829 A5 JPWO2021100829 A5 JP WO2021100829A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- positive
- cell aggregate
- cell
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cell Anatomy 0.000 claims 87
- 238000000034 method Methods 0.000 claims 38
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims 16
- 239000000203 mixture Substances 0.000 claims 15
- VYFYYTLLBUKUHU-UHFFFAOYSA-N dopamine Chemical compound NCCC1=CC=C(O)C(O)=C1 VYFYYTLLBUKUHU-UHFFFAOYSA-N 0.000 claims 14
- 210000000653 nervous system Anatomy 0.000 claims 13
- 239000003761 preservation solution Substances 0.000 claims 13
- 238000002054 transplantation Methods 0.000 claims 12
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims 11
- 238000007710 freezing Methods 0.000 claims 9
- 230000008014 freezing Effects 0.000 claims 9
- 102100029284 Hepatocyte nuclear factor 3-beta Human genes 0.000 claims 8
- 101001062347 Homo sapiens Hepatocyte nuclear factor 3-beta Proteins 0.000 claims 8
- 101001109698 Homo sapiens Nuclear receptor subfamily 4 group A member 2 Proteins 0.000 claims 7
- 102100022676 Nuclear receptor subfamily 4 group A member 2 Human genes 0.000 claims 7
- 229960003638 dopamine Drugs 0.000 claims 7
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims 6
- 101001038339 Homo sapiens LIM homeobox transcription factor 1-alpha Proteins 0.000 claims 6
- 102100040290 LIM homeobox transcription factor 1-alpha Human genes 0.000 claims 6
- 210000005155 neural progenitor cell Anatomy 0.000 claims 6
- 239000002577 cryoprotective agent Substances 0.000 claims 5
- 235000013772 propylene glycol Nutrition 0.000 claims 5
- 239000000243 solution Substances 0.000 claims 5
- 238000010257 thawing Methods 0.000 claims 5
- 239000003814 drug Substances 0.000 claims 4
- 210000002569 neuron Anatomy 0.000 claims 4
- 229940124597 therapeutic agent Drugs 0.000 claims 4
- 239000004480 active ingredient Substances 0.000 claims 3
- 238000001816 cooling Methods 0.000 claims 3
- 238000005138 cryopreservation Methods 0.000 claims 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 3
- 238000004519 manufacturing process Methods 0.000 claims 3
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims 2
- XPFCZYUVICHKDS-UHFFFAOYSA-N 3-methylbutane-1,3-diol Chemical compound CC(C)(O)CCO XPFCZYUVICHKDS-UHFFFAOYSA-N 0.000 claims 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 2
- 238000012258 culturing Methods 0.000 claims 2
- SZXQTJUDPRGNJN-UHFFFAOYSA-N dipropylene glycol Chemical compound OCCCOCCCO SZXQTJUDPRGNJN-UHFFFAOYSA-N 0.000 claims 2
- 201000010099 disease Diseases 0.000 claims 2
- 230000003291 dopaminomimetic effect Effects 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 239000007788 liquid Substances 0.000 claims 2
- 230000007774 longterm Effects 0.000 claims 2
- 210000001259 mesencephalon Anatomy 0.000 claims 2
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 2
- 238000011084 recovery Methods 0.000 claims 2
- 230000008929 regeneration Effects 0.000 claims 2
- 238000011069 regeneration method Methods 0.000 claims 2
- 238000003860 storage Methods 0.000 claims 2
- DNIAPMSPPWPWGF-VKHMYHEASA-N (+)-propylene glycol Chemical compound C[C@H](O)CO DNIAPMSPPWPWGF-VKHMYHEASA-N 0.000 claims 1
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 claims 1
- YPFDHNVEDLHUCE-UHFFFAOYSA-N 1,3-propanediol Substances OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 claims 1
- 208000024827 Alzheimer disease Diseases 0.000 claims 1
- 101000652332 Homo sapiens Transcription factor SOX-1 Proteins 0.000 claims 1
- 208000023105 Huntington disease Diseases 0.000 claims 1
- 206010062767 Hypophysitis Diseases 0.000 claims 1
- 208000026072 Motor neurone disease Diseases 0.000 claims 1
- 208000001089 Multiple system atrophy Diseases 0.000 claims 1
- 108010032788 PAX6 Transcription Factor Proteins 0.000 claims 1
- 102100037506 Paired box protein Pax-6 Human genes 0.000 claims 1
- 208000018737 Parkinson disease Diseases 0.000 claims 1
- 206010034010 Parkinsonism Diseases 0.000 claims 1
- 208000007014 Retinitis pigmentosa Diseases 0.000 claims 1
- 208000010112 Spinocerebellar Degenerations Diseases 0.000 claims 1
- 208000011641 Spinocerebellar disease Diseases 0.000 claims 1
- 102100030248 Transcription factor SOX-1 Human genes 0.000 claims 1
- 206010064930 age-related macular degeneration Diseases 0.000 claims 1
- 206010002026 amyotrophic lateral sclerosis Diseases 0.000 claims 1
- 210000003169 central nervous system Anatomy 0.000 claims 1
- 210000001638 cerebellum Anatomy 0.000 claims 1
- 210000004720 cerebrum Anatomy 0.000 claims 1
- 239000013078 crystal Substances 0.000 claims 1
- 210000002451 diencephalon Anatomy 0.000 claims 1
- 210000005064 dopaminergic neuron Anatomy 0.000 claims 1
- 235000011187 glycerol Nutrition 0.000 claims 1
- 210000003016 hypothalamus Anatomy 0.000 claims 1
- 208000002780 macular degeneration Diseases 0.000 claims 1
- 208000005264 motor neuron disease Diseases 0.000 claims 1
- 201000006417 multiple sclerosis Diseases 0.000 claims 1
- 210000005036 nerve Anatomy 0.000 claims 1
- 210000003061 neural cell Anatomy 0.000 claims 1
- 208000015122 neurodegenerative disease Diseases 0.000 claims 1
- 230000014511 neuron projection development Effects 0.000 claims 1
- 210000003635 pituitary gland Anatomy 0.000 claims 1
- 229920000166 polytrimethylene carbonate Polymers 0.000 claims 1
- 239000002243 precursor Substances 0.000 claims 1
- 238000004321 preservation Methods 0.000 claims 1
- 210000004129 prosencephalon Anatomy 0.000 claims 1
- 210000001525 retina Anatomy 0.000 claims 1
- 210000001202 rhombencephalon Anatomy 0.000 claims 1
- 210000000278 spinal cord Anatomy 0.000 claims 1
- 208000020431 spinal cord injury Diseases 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 210000001587 telencephalon Anatomy 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2024075610A JP7787527B2 (ja) | 2019-11-20 | 2024-05-08 | 神経系細胞の凍結方法 |
| JP2025205398A JP2026026215A (ja) | 2019-11-20 | 2025-11-27 | 神経系細胞の凍結方法 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2019209929 | 2019-11-20 | ||
| JP2019209929 | 2019-11-20 | ||
| PCT/JP2020/043275 WO2021100829A1 (ja) | 2019-11-20 | 2020-11-19 | 神経系細胞の凍結方法 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024075610A Division JP7787527B2 (ja) | 2019-11-20 | 2024-05-08 | 神経系細胞の凍結方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JPWO2021100829A1 JPWO2021100829A1 (https=) | 2021-05-27 |
| JPWO2021100829A5 true JPWO2021100829A5 (https=) | 2023-10-20 |
| JP7491533B2 JP7491533B2 (ja) | 2024-05-28 |
Family
ID=75980133
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021558457A Active JP7491533B2 (ja) | 2019-11-20 | 2020-11-19 | 神経系細胞の凍結方法 |
| JP2024075610A Active JP7787527B2 (ja) | 2019-11-20 | 2024-05-08 | 神経系細胞の凍結方法 |
| JP2025205398A Pending JP2026026215A (ja) | 2019-11-20 | 2025-11-27 | 神経系細胞の凍結方法 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024075610A Active JP7787527B2 (ja) | 2019-11-20 | 2024-05-08 | 神経系細胞の凍結方法 |
| JP2025205398A Pending JP2026026215A (ja) | 2019-11-20 | 2025-11-27 | 神経系細胞の凍結方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20230000071A1 (https=) |
| EP (1) | EP4063496A4 (https=) |
| JP (3) | JP7491533B2 (https=) |
| KR (1) | KR20220104764A (https=) |
| CN (1) | CN115175989A (https=) |
| AU (1) | AU2020387259A1 (https=) |
| CA (1) | CA3162268A1 (https=) |
| IL (1) | IL293137A (https=) |
| MY (1) | MY209020A (https=) |
| WO (1) | WO2021100829A1 (https=) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2024293098A1 (en) * | 2023-07-14 | 2026-02-12 | Advanced Biologics, Llc | Increasing viability of cells within a frozen cellular implant |
| JP7840913B2 (ja) * | 2023-09-15 | 2026-04-06 | 株式会社クラレ | 細胞凝集体および細胞凝集体の製造方法 |
| US20250157580A1 (en) * | 2023-11-13 | 2025-05-15 | Aspen Neuroscience, Inc. | Methods of predicting dopamine production capability of differentiated neuronal cells |
| JP2025094837A (ja) * | 2023-12-13 | 2025-06-25 | 国立大学法人京都大学 | 凍結細胞の製造方法 |
| WO2025166041A1 (en) * | 2024-01-31 | 2025-08-07 | BrainXell Therapeutics, Inc. | Methods for preserving neural progenitor cell survival in vitro and in vivo |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1993007745A1 (en) * | 1991-10-23 | 1993-04-29 | Cellpro, Incorporated | Method for freezing engrafting cells |
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| NZ518601A (en) | 1999-09-24 | 2004-10-29 | Morphogen Pharmaceuticals Inc | Pluripotent embryonic-like stem cells, compositions, methods and uses thereof |
| US6280718B1 (en) | 1999-11-08 | 2001-08-28 | Wisconsin Alumni Reasearch Foundation | Hematopoietic differentiation of human pluripotent embryonic stem cells |
| WO2002101057A1 (fr) | 2001-06-08 | 2002-12-19 | Dnavec Research Inc. | Transfert genique dans des cellules souches embryonnaires de primate a l'aide d'un virus de l'immunodeficience simienne de pseudo type vsv-g utilise comme vecteur |
| JP2008501320A (ja) * | 2004-06-02 | 2008-01-24 | イーエス・セル・インターナショナル・プライヴェート・リミテッド | 細胞保存方法 |
| US7811819B2 (en) * | 2005-05-31 | 2010-10-12 | The University Of Washington | Cryopreservation of primate embryonic stem cells |
| JPWO2009148170A1 (ja) | 2008-06-06 | 2011-11-04 | 独立行政法人理化学研究所 | 幹細胞の培養方法 |
| JP6210881B2 (ja) | 2011-10-31 | 2017-10-11 | 国立研究開発法人理化学研究所 | 幹細胞の培養方法 |
| JP6012164B2 (ja) * | 2011-11-25 | 2016-10-25 | 住友化学株式会社 | 多能性幹細胞由来の組織の凍結保存方法 |
| US20150159133A1 (en) | 2012-06-11 | 2015-06-11 | Lonza Walkersville, Inc. | Method of in vitro differentiation of motor neuron progenitors (mnps) from human induced pluripotent stem cells and cryopreservation of mnps |
| AU2014316100B2 (en) | 2013-09-05 | 2020-11-05 | Eisai R&D Management Co., Ltd. | New method for inducing dopamine-producing neural precursor cells |
| ES2732730T3 (es) | 2013-11-22 | 2019-11-25 | Riken | Método para producir telencéfalo o tejido progenitor del mismo |
| CA2956130C (en) | 2014-07-25 | 2023-09-26 | Riken | Method for producing adenohypophysis or precursor tissue thereof |
| US10781420B2 (en) | 2014-09-08 | 2020-09-22 | Riken | Method for producing cerebellar progenitor tissue |
| LU92845B1 (en) * | 2015-10-08 | 2017-05-02 | Univ Du Luxembourg Campus Belval | Means and methods for generating midbrain organoids |
| JP6820531B2 (ja) | 2015-12-10 | 2021-01-27 | 学校法人慶應義塾 | ヒトｉＰＳ細胞由来神経幹細胞／前駆細胞の凍結方法 |
| CN108495929B (zh) | 2016-01-22 | 2023-04-18 | 国立大学法人名古屋大学 | 由人多能干细胞向下丘脑神经元的分化诱导 |
| WO2017159862A1 (ja) | 2016-03-18 | 2017-09-21 | 国立大学法人京都大学 | 多能性幹細胞由来心筋細胞の凝集体の凍結方法 |
| CN105850980B (zh) * | 2016-04-14 | 2018-06-12 | 广州赛莱拉干细胞科技股份有限公司 | 一种角膜缘干细胞的冻存液及冻存方法 |
| JP7011260B2 (ja) * | 2016-04-22 | 2022-02-10 | 国立大学法人京都大学 | ドーパミン産生神経前駆細胞の製造方法 |
| US20200405768A1 (en) * | 2018-02-19 | 2020-12-31 | Sumitomo Dainippon Pharma Co., Ltd. | Cell Aggregate, Mixture of Cell Aggregates, and Method for Preparing Same |
-
2020
- 2020-11-19 AU AU2020387259A patent/AU2020387259A1/en active Pending
- 2020-11-19 CN CN202080093441.4A patent/CN115175989A/zh active Pending
- 2020-11-19 WO PCT/JP2020/043275 patent/WO2021100829A1/ja not_active Ceased
- 2020-11-19 JP JP2021558457A patent/JP7491533B2/ja active Active
- 2020-11-19 EP EP20890883.0A patent/EP4063496A4/en active Pending
- 2020-11-19 MY MYPI2022002608A patent/MY209020A/en unknown
- 2020-11-19 US US17/778,250 patent/US20230000071A1/en active Pending
- 2020-11-19 CA CA3162268A patent/CA3162268A1/en active Pending
- 2020-11-19 KR KR1020227020620A patent/KR20220104764A/ko active Pending
- 2020-11-19 IL IL293137A patent/IL293137A/en unknown
-
2024
- 2024-05-08 JP JP2024075610A patent/JP7787527B2/ja active Active
-
2025
- 2025-11-27 JP JP2025205398A patent/JP2026026215A/ja active Pending
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