JPWO2020135651A5 - - Google Patents
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- Publication number
- JPWO2020135651A5 JPWO2020135651A5 JP2021534996A JP2021534996A JPWO2020135651A5 JP WO2020135651 A5 JPWO2020135651 A5 JP WO2020135651A5 JP 2021534996 A JP2021534996 A JP 2021534996A JP 2021534996 A JP2021534996 A JP 2021534996A JP WO2020135651 A5 JPWO2020135651 A5 JP WO2020135651A5
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- stranded dna
- restriction endonuclease
- target single
- type
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims 22
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims 14
- 108020004682 Single-Stranded DNA Proteins 0.000 claims 12
- 108091007521 restriction endonucleases Proteins 0.000 claims 11
- 239000002773 nucleotide Substances 0.000 claims 9
- 125000003729 nucleotide group Chemical group 0.000 claims 9
- 230000000295 complement Effects 0.000 claims 8
- 229940035893 Uracil Drugs 0.000 claims 7
- 229920002395 Aptamer Polymers 0.000 claims 5
- 101700011961 DPOM Proteins 0.000 claims 5
- 101710029649 MDV043 Proteins 0.000 claims 5
- 229920001850 Nucleic acid sequence Polymers 0.000 claims 5
- 101700061424 POLB Proteins 0.000 claims 5
- 101700054624 RF1 Proteins 0.000 claims 5
- 239000003153 chemical reaction reagent Substances 0.000 claims 5
- 238000003776 cleavage reaction Methods 0.000 claims 5
- 230000003044 adaptive Effects 0.000 claims 4
- 101710030587 ligN Proteins 0.000 claims 4
- 101700077585 ligd Proteins 0.000 claims 4
- 102000003960 Ligases Human genes 0.000 claims 3
- 108090000364 Ligases Proteins 0.000 claims 3
- 230000003321 amplification Effects 0.000 claims 3
- 238000003199 nucleic acid amplification method Methods 0.000 claims 3
- 238000005096 rolling process Methods 0.000 claims 3
- UDMBCSSLTHHNCD-KQYNXXCUSA-N Adenosine monophosphate Chemical group C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H]1O UDMBCSSLTHHNCD-KQYNXXCUSA-N 0.000 claims 2
- 229950006790 Adenosine phosphate Drugs 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- DJJCXFVJDGTHFX-XVFCMESISA-N Uridine monophosphate Chemical group O[C@@H]1[C@H](O)[C@@H](COP(O)(O)=O)O[C@H]1N1C(=O)NC(=O)C=C1 DJJCXFVJDGTHFX-XVFCMESISA-N 0.000 claims 2
- GYOZYWVXFNDGLU-XLPZGREQSA-N dTMP Chemical group O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(O)=O)[C@@H](O)C1 GYOZYWVXFNDGLU-XLPZGREQSA-N 0.000 claims 2
- 229920000972 Sense strand Polymers 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 238000000137 annealing Methods 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 238000003752 polymerase chain reaction Methods 0.000 claims 1
- 102220174584 rs2228570 Human genes 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811624165.0 | 2018-12-28 | ||
| CN201811624165 | 2018-12-28 | ||
| PCT/CN2019/128948 WO2020135651A1 (zh) | 2018-12-28 | 2019-12-27 | 一种单链dna合成方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2022515085A JP2022515085A (ja) | 2022-02-17 |
| JPWO2020135651A5 true JPWO2020135651A5 (ko) | 2023-01-11 |
Family
ID=71126836
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021534996A Pending JP2022515085A (ja) | 2018-12-28 | 2019-12-27 | 一本鎖ｄｎａの合成方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20220042059A1 (ko) |
| EP (1) | EP3904511A4 (ko) |
| JP (1) | JP2022515085A (ko) |
| KR (1) | KR20210110790A (ko) |
| CN (1) | CN113227370B (ko) |
| IL (1) | IL283989A (ko) |
| TW (1) | TW202039838A (ko) |
| WO (1) | WO2020135651A1 (ko) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115605590A (zh) * | 2021-02-10 | 2023-01-13 | 清华大学（Cn） | 一种制备定点修饰的长链dna的方法 |
| WO2023072145A1 (zh) | 2021-10-26 | 2023-05-04 | 南京金斯瑞生物科技有限公司 | 一种纯化单链dna的方法 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0610045D0 (en) * | 2006-05-19 | 2006-06-28 | Plant Bioscience Ltd | Improved uracil-excision based molecular cloning |
| US9540637B2 (en) * | 2008-01-09 | 2017-01-10 | Life Technologies Corporation | Nucleic acid adaptors and uses thereof |
| WO2009120374A2 (en) * | 2008-03-28 | 2009-10-01 | Pacific Biosciences Of California, Inc. | Methods and compositions for nucleic acid sample preparation |
| AU2010282225A1 (en) * | 2009-08-12 | 2012-04-05 | Commonwealth Scientific And Industrial Research Organisation | Detection and analysis of methylation in nucleic acid sequences |
| CN102296065B (zh) * | 2011-08-04 | 2013-05-15 | 盛司潼 | 用于构建测序文库的系统与方法 |
| CN102719550A (zh) * | 2012-07-10 | 2012-10-10 | 中国人民解放军第三军医大学第一附属医院 | 一种基于分裂锁式探针的多重rca方法 |
| CN103255227B (zh) * | 2013-05-30 | 2015-02-25 | 上海快灵生物科技有限公司 | 引物介导环化的恒温核酸滚环扩增方法及试剂盒 |
| US20180044668A1 (en) * | 2014-10-14 | 2018-02-15 | Bgi Shenzhen Co., Limited | Mate pair library construction |
| CN105624165B (zh) * | 2016-01-05 | 2018-11-30 | 山东大学 | 基于自锁式适体探针的级联扩增策略的生物分子检测方法 |
| CN109689872B (zh) * | 2016-11-21 | 2022-12-23 | 深圳华大智造科技股份有限公司 | 一种dna末端修复与加a的方法 |
| WO2018121634A1 (zh) * | 2016-12-30 | 2018-07-05 | 安诺优达基因科技(北京)有限公司 | 用于dna片段的非特异性复制的方法及试剂盒 |
| CN108060191B (zh) * | 2017-11-07 | 2021-05-04 | 深圳华大智造科技股份有限公司 | 一种双链核酸片段加接头的方法、文库构建方法和试剂盒 |
-
2019
- 2019-12-27 TW TW108147968A patent/TW202039838A/zh unknown
- 2019-12-27 JP JP2021534996A patent/JP2022515085A/ja active Pending
- 2019-12-27 EP EP19903928.0A patent/EP3904511A4/en active Pending
- 2019-12-27 US US17/309,488 patent/US20220042059A1/en active Pending
- 2019-12-27 WO PCT/CN2019/128948 patent/WO2020135651A1/zh unknown
- 2019-12-27 KR KR1020217014403A patent/KR20210110790A/ko unknown
- 2019-12-27 CN CN201980086128.5A patent/CN113227370B/zh active Active
-
2021
- 2021-06-14 IL IL283989A patent/IL283989A/en unknown
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