JPWO2012002541A1 - 標的分子の検出法 - Google Patents

標的分子の検出法 Download PDF

Info

Publication number
JPWO2012002541A1
JPWO2012002541A1 JP2011548712A JP2011548712A JPWO2012002541A1 JP WO2012002541 A1 JPWO2012002541 A1 JP WO2012002541A1 JP 2011548712 A JP2011548712 A JP 2011548712A JP 2011548712 A JP2011548712 A JP 2011548712A JP WO2012002541 A1 JPWO2012002541 A1 JP WO2012002541A1
Authority
JP
Japan
Prior art keywords
target molecule
nucleic acid
oligonucleotide
sequence
population
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2011548712A
Other languages
English (en)
Japanese (ja)
Inventor
真 宮岸
真 宮岸
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
National Institute of Advanced Industrial Science and Technology AIST
Original Assignee
National Institute of Advanced Industrial Science and Technology AIST
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by National Institute of Advanced Industrial Science and Technology AIST filed Critical National Institute of Advanced Industrial Science and Technology AIST
Publication of JPWO2012002541A1 publication Critical patent/JPWO2012002541A1/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6811—Selection methods for production or design of target specific oligonucleotides or binding molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2011548712A 2010-07-01 2011-07-01 標的分子の検出法 Pending JPWO2012002541A1 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2010151242 2010-07-01
JP2010151242 2010-07-01
PCT/JP2011/065205 WO2012002541A1 (ja) 2010-07-01 2011-07-01 標的分子の検出法

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2012110792A Division JP2012198225A (ja) 2010-07-01 2012-05-14 標的分子の検出法

Publications (1)

Publication Number Publication Date
JPWO2012002541A1 true JPWO2012002541A1 (ja) 2013-08-29

Family

ID=45402243

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2011548712A Pending JPWO2012002541A1 (ja) 2010-07-01 2011-07-01 標的分子の検出法
JP2012110792A Pending JP2012198225A (ja) 2010-07-01 2012-05-14 標的分子の検出法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2012110792A Pending JP2012198225A (ja) 2010-07-01 2012-05-14 標的分子の検出法

Country Status (4)

Country Link
US (1) US20130116129A1 (enExample)
EP (1) EP2589657A4 (enExample)
JP (2) JPWO2012002541A1 (enExample)
WO (1) WO2012002541A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102971422B (zh) * 2010-07-02 2015-07-22 国立大学法人东京农工大学 Psa结合适体和前列腺癌的诊断方法
JP6183917B2 (ja) * 2012-03-23 2017-08-23 Ｎｅｃソリューションイノベータ株式会社 ストレプトアビジンの分析用デバイスおよび分析方法
JP5999786B2 (ja) 2012-03-23 2016-09-28 Ｎｅｃソリューションイノベータ株式会社 Ａｔｐまたはａｍｐの分析用デバイスおよび分析方法
US20150292005A1 (en) * 2012-04-27 2015-10-15 Kirin Kabushiki Kaisha Nucleic acid molecules for highly sensitive detection of ligands, screening method for nucleic acid molecules, and optimization method for sensitivity of nucleic acid molecules
US10942184B2 (en) 2012-10-23 2021-03-09 Caris Science, Inc. Aptamers and uses thereof
AU2013340414B2 (en) 2012-10-23 2019-05-02 Caris Science, Inc. Aptamers and uses thereof
EP2935628B1 (en) 2012-12-19 2018-03-21 Caris Life Sciences Switzerland Holdings GmbH Compositions and methods for aptamer screening
US9828599B2 (en) * 2013-09-18 2017-11-28 General Electric Company Methods of selecting binding-elements and uses thereof
US10359419B2 (en) 2013-10-02 2019-07-23 General Electric Company Methods for detection of target using affinity binding
WO2015083731A1 (ja) * 2013-12-04 2015-06-11 独立行政法人産業技術総合研究所 マイクロベシクルに対する核酸アプタマー
CN105483267B (zh) * 2016-01-15 2018-12-04 古博 血浆游离DNA双分子标记、标记和检测血浆cfDNA的方法及其用途
JP7146839B2 (ja) * 2019-11-15 2022-10-04 花王株式会社 シーケンシング用ライブラリの調製方法
EP4083278A4 (en) 2019-11-15 2024-02-28 Kao Corporation METHOD FOR CREATING A SEQUENCING LIBRARY
CN113699215B (zh) * 2021-08-13 2024-06-14 东南大学 一种核酸适体筛选方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997038134A1 (en) * 1996-04-05 1997-10-16 Nexstar Pharmaceuticals, Inc. Method for detecting a target compound using a nucleic acid ligand
JP2008102675A (ja) * 2006-10-18 2008-05-01 Nec Soft Ltd 塩基配列の同定方法及び核酸分子の二次構造取得方法、並びにこれらを実行する装置及びプログラム
WO2009053691A1 (en) * 2007-10-22 2009-04-30 Pronota Nv Method of selecting aptamers
JP2009189310A (ja) * 2008-02-15 2009-08-27 Olympus Corp 高親和性核酸フラグメント群の塩基配列決定方法および核酸アプタマーのスクリーニング方法
WO2009107803A1 (ja) * 2008-02-27 2009-09-03 ソニー株式会社 標的物質の定量方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5270163A (en) 1990-06-11 1993-12-14 University Research Corporation Methods for identifying nucleic acid ligands
CA2084987C (en) * 1990-06-11 2007-02-13 Gilead Sciences, Inc. Nucleic acid ligands
US20090053138A1 (en) * 2002-11-21 2009-02-26 Preiss Jeffrey R Stabilized Aptamers to Platelet Derived Growth Factor and their Use as Oncology Therapeutics
JP2008161165A (ja) 2006-12-28 2008-07-17 Dnaform:Kk 競合オリゴヌクレオチドを用いた遺伝子検出法
EP2209893B1 (en) * 2007-10-12 2013-11-20 Pronota NV Use of aptamers in proteomics
CN102066562A (zh) * 2008-03-12 2011-05-18 雪城大学 结构限定的适配子的直接选择
JP2010041951A (ja) 2008-08-11 2010-02-25 Olympus Corp 標的分子の検出方法
WO2010019969A1 (en) * 2008-08-15 2010-02-18 Cornell University Device for rapid identification of nucleic acids for binding to specific chemical targets
JP5256016B2 (ja) 2008-12-25 2013-08-07 コスモ工機株式会社 管接続部材の脱着装置
JP5419145B2 (ja) * 2009-07-24 2014-02-19 Ｎｅｃソフト株式会社 アプタマー分類装置、アプタマー分類方法、プログラムおよび記録媒体

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997038134A1 (en) * 1996-04-05 1997-10-16 Nexstar Pharmaceuticals, Inc. Method for detecting a target compound using a nucleic acid ligand
JP2008102675A (ja) * 2006-10-18 2008-05-01 Nec Soft Ltd 塩基配列の同定方法及び核酸分子の二次構造取得方法、並びにこれらを実行する装置及びプログラム
WO2009053691A1 (en) * 2007-10-22 2009-04-30 Pronota Nv Method of selecting aptamers
JP2009189310A (ja) * 2008-02-15 2009-08-27 Olympus Corp 高親和性核酸フラグメント群の塩基配列決定方法および核酸アプタマーのスクリーニング方法
WO2009107803A1 (ja) * 2008-02-27 2009-09-03 ソニー株式会社 標的物質の定量方法

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
JPN6012011892; ＢＭＣ Ｂｉｏｉｎｆｏｒｍａｔｉｃｓ Ｖｏｌ．９, 2008, ｄｏｉ：１０．１１８６／１４７１-２１０５-９-１７６ *
JPN6012011894; Ｇｅｎｏｍｅ Ｒｅｓ． Ｖｏｌ．２０， Ｎｏ．６, 201006, ｐｐ．８６１-８７３ *
JPN6012011896; ＢＭＣ Ｂｉｏｉｎｆｏｒｍａｔｉｃｓ Ｖｏｌ．７， Ｓｕｐｐｌ．２, 2006, ｄｏｉ：１０．１１８６／１４７１-２１０５-７-Ｓ２-Ｓ６ *
JPN6012011898; Ｂｉｏｔｅｃｈｎｏｌ． Ｌｅｔｔ． Ｖｏｌ．３２， Ｎｏ．３, 201003, ｐｐ．３７９-３８５ *
JPN6012011901; Ｂｉｏｍｏｌ． Ｅｎｇ． Ｖｏｌ．２４， Ｎｏ．２, 2007, ｐｐ．１７９-１８９ *
JPN6012011907; Ｃｌｉｎ． Ｃｈｅｍ． Ｖｏｌ．５５， Ｎｏ．９, 200909, ｐｐ．１６８６-１６９３ *

Also Published As

Publication number Publication date
JP2012198225A (ja) 2012-10-18
US20130116129A1 (en) 2013-05-09
EP2589657A4 (en) 2014-04-23
EP2589657A1 (en) 2013-05-08
EP2589657A9 (en) 2013-10-16
WO2012002541A1 (ja) 2012-01-05

Similar Documents

Publication Publication Date Title
JPWO2012002541A1 (ja) 標的分子の検出法
US10570448B2 (en) Compositions and methods for identification of a duplicate sequencing read
Thiviyanathan et al. Aptamers and the next generation of diagnostic reagents
US20030138831A1 (en) Method for sequencing and characterizing polymeric biomolecules using aptamers and a method for producing aptamers
CN114990126B (zh) 特异性结合Bst DNA聚合酶大片段活性位点的核酸适配体及应用
JP7684984B2 (ja) ローリングサークル増幅産物をマッピングするための方法
KR20180041331A (ko) 분자결합핵산 선정과 표적분자 동정 방법 및 키드, 그리고 그들의 용도
CN114364813B (zh) 多重等温扩增核酸序列的方法
JP2025535369A (ja) シーケンシングライブラリー正規化のための方法および組成物
EP3536803A1 (en) Quantitative cluster analysis method of target protein by using next-generation sequencing and use thereof
CN113832216A (zh) 使用发卡寡核苷酸扩增核酸的方法
US10590451B2 (en) Methods of constructing a circular template and detecting DNA molecules
CN117487813B (zh) 特异性识别阿奇霉素的单链dna适配体序列及其应用
US12215378B2 (en) RNA identity method using RNAse H digestion and size fractionating
JP2009195187A (ja) 核酸アプタマーのスクリーニング方法
US20220220536A1 (en) Compositions and methods of using a dna nanoswitch for the detection of rna
Dausse et al. In vitro selection procedures for identifying DNA and RNA aptamers targeted to nucleic acids and proteins
KR102403628B1 (ko) 시료의 표적분자 집단에 대한 프로파일을 얻는 방법
JP2020096592A (ja) 亜硝酸酸化細菌に結合する核酸分子並びに核酸分子を用いた亜硝酸酸化細菌の検出方法、検出キット及び検出試薬
Ulrich et al. RNA and DNA aptamers in cytomics analysis
US20240384336A1 (en) Optimized Set Of Oligonucleotides For Bulk RNA Barcoding And Sequencing
JP2012143197A (ja) Ｄｎａアプタマーの選択方法およびｄｎａアプタマー
JP2025133705A (ja) 核酸の正確な並列定量のための高感な方法
CN115175985A (zh) 从未经处理的生物样本中提取单链dna和rna并测序的方法
JP2023524129A (ja) 反応混合物からの過剰なオリゴヌクレオチドの除去

Legal Events

Date Code Title Description
A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20120821