JPWO2009136432A1 - 糖質定量方法および糖質定量キット - Google Patents
糖質定量方法および糖質定量キット Download PDFInfo
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- 150000001720 carbohydrates Chemical class 0.000 title claims abstract description 132
- 238000000034 method Methods 0.000 title claims abstract description 84
- 235000014633 carbohydrates Nutrition 0.000 title description 94
- 235000000346 sugar Nutrition 0.000 title description 12
- 102000038379 digestive enzymes Human genes 0.000 claims abstract description 74
- 108091007734 digestive enzymes Proteins 0.000 claims abstract description 74
- 238000011002 quantification Methods 0.000 claims abstract description 55
- 241001465754 Metazoa Species 0.000 claims abstract description 41
- 238000006243 chemical reaction Methods 0.000 claims abstract description 29
- 102000004139 alpha-Amylases Human genes 0.000 claims abstract description 11
- 108090000637 alpha-Amylases Proteins 0.000 claims abstract description 11
- 229940024171 alpha-amylase Drugs 0.000 claims abstract description 11
- 108091005804 Peptidases Proteins 0.000 claims abstract description 10
- 239000004365 Protease Substances 0.000 claims abstract description 10
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 claims abstract description 9
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims abstract description 9
- 102000004157 Hydrolases Human genes 0.000 claims description 21
- 108090000604 Hydrolases Proteins 0.000 claims description 21
- 230000000968 intestinal effect Effects 0.000 claims description 19
- 229920001542 oligosaccharide Polymers 0.000 description 41
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
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- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
- G01N2333/926—Hydrolases (3) acting on glycosyl compounds (3.2) acting on alpha -1, 4-glucosidic bonds, e.g. hyaluronidase, invertase, amylase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
- G01N2333/926—Hydrolases (3) acting on glycosyl compounds (3.2) acting on alpha -1, 4-glucosidic bonds, e.g. hyaluronidase, invertase, amylase
- G01N2333/934—Glucoamylase
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
図1は、本発明の第一実施例にかかる糖質定量方法の操作手順図を示したものである。より具体的には、図1は、本実施例にかかる酵素−HPLC法による糖質定量の操作手順図を示したものである。
カラム Shodex SUGAR KS−802
(8.0φ×300mm,昭和電工(株)製)
カラム温度 70℃
移動相 H2O
流速 0.5mL/min
試料流入量 10μL
検出器 示差屈折計 RID−10A((株)島津製作所製)
未消化物の回収率(%)=(((A/B)×希釈倍率)/試料重量(g))×100
ここで、A:酵素処理後の糖質のピークエリア
B:標準物質溶液のピークエリア
なお、本発明は、上記実施形態および実施例(以下「実施形態等」という。)に限定されるものではなく、必要に応じて種々の変更を加えて実施することも可能であり、それらはいずれも本発明の技術的範囲に含まれる。
Claims (5)
- 消化酵素を用いた糖質定量方法であって、
前記消化酵素として、動物由来の低分子糖質消化酵素が用いられる
ことを特徴とする糖質定量方法。 - 消化酵素を用いた糖質定量方法であって、
耐熱性α−アミラーゼを用いた第一反応工程と、
プロテアーゼおよびアミログルコシダーゼを用いた第二反応工程と、
動物由来の低分子糖質消化酵素を用いた第三反応工程とを備えた
ことを特徴とする糖質定量方法。 - 前記低分子糖質消化酵素として、動物由来の小腸粘膜水解酵素が用いられる
請求項1または2に記載の糖質定量方法。 - 消化酵素を有する糖質定量キットであって、
前記消化酵素として、動物由来の低分子糖質消化酵素を備えた
ことを特徴とする糖質定量キット。 - 前記消化酵素として、動物由来の小腸粘膜水解酵素を備えた
請求項4に記載の糖質定量キット。
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP2008/058440 WO2009136432A1 (ja) | 2008-05-06 | 2008-05-06 | 糖質定量方法および糖質定量キット |
Publications (2)
Publication Number | Publication Date |
---|---|
JPWO2009136432A1 true JPWO2009136432A1 (ja) | 2011-09-01 |
JP5532256B2 JP5532256B2 (ja) | 2014-06-25 |
Family
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Application Number | Title | Priority Date | Filing Date |
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JP2010510973A Active JP5532256B2 (ja) | 2008-05-06 | 2008-05-06 | 糖質定量方法および糖質定量キット |
Country Status (3)
Country | Link |
---|---|
US (1) | US8367365B2 (ja) |
JP (1) | JP5532256B2 (ja) |
WO (1) | WO2009136432A1 (ja) |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0873482A (ja) * | 1994-06-27 | 1996-03-19 | Hayashibara Biochem Lab Inc | 還元性を低減させた糖質とその製造方法並びに用途 |
JPH10150934A (ja) * | 1996-11-20 | 1998-06-09 | Matsutani Chem Ind Ltd | 還元難消化性水飴およびこれを用いた食品 |
WO2006035725A1 (ja) * | 2004-09-27 | 2006-04-06 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | イソサイクロマルトオリゴ糖及びイソサイクロマルトオリゴ糖生成酵素とそれらの製造方法並びに用途 |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3249514A (en) * | 1963-11-18 | 1966-05-03 | Bode Harold Eli | Production and use of amyloglucosidase |
GB1446965A (en) * | 1974-02-14 | 1976-08-18 | Agricultural Vegetable Prod | Preparation of food products |
JP3183500B2 (ja) | 1989-04-10 | 2001-07-09 | 日本化薬株式会社 | 糖アルコールの定量方法 |
TW426737B (en) | 1994-06-27 | 2001-03-21 | Hayashibara Biochem Lab | Saccharide composition with reduced reducibility, and preparation and uses thereof |
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2008
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Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
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JPH0873482A (ja) * | 1994-06-27 | 1996-03-19 | Hayashibara Biochem Lab Inc | 還元性を低減させた糖質とその製造方法並びに用途 |
JPH10150934A (ja) * | 1996-11-20 | 1998-06-09 | Matsutani Chem Ind Ltd | 還元難消化性水飴およびこれを用いた食品 |
WO2006035725A1 (ja) * | 2004-09-27 | 2006-04-06 | Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo | イソサイクロマルトオリゴ糖及びイソサイクロマルトオリゴ糖生成酵素とそれらの製造方法並びに用途 |
Non-Patent Citations (2)
Title |
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"Analytical and Bioanalytical Chemistry", 2007, VOL.389, NO.1, PP.291-308, JPN6013004513, ISSN: 0002748834 * |
"日本食品科学工学会誌", 2004年、第51巻、第1号、第28頁−第33頁, JPN6013004510, ISSN: 0002447448 * |
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US8367365B2 (en) | 2013-02-05 |
JP5532256B2 (ja) | 2014-06-25 |
WO2009136432A1 (ja) | 2009-11-12 |
US20110059474A1 (en) | 2011-03-10 |
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