JPWO2005001086A1 - 固定化ｍＲＮＡ−ピューロマイシン連結体及びその用途 - Google Patents

固定化ｍＲＮＡ−ピューロマイシン連結体及びその用途 Download PDF

Info

Publication number
JPWO2005001086A1
JPWO2005001086A1 JP2005511126A JP2005511126A JPWO2005001086A1 JP WO2005001086 A1 JPWO2005001086 A1 JP WO2005001086A1 JP 2005511126 A JP2005511126 A JP 2005511126A JP 2005511126 A JP2005511126 A JP 2005511126A JP WO2005001086 A1 JPWO2005001086 A1 JP WO2005001086A1
Authority
JP
Japan
Prior art keywords
mrna
puromycin
protein
conjugate
solid phase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2005511126A
Other languages
English (en)
Japanese (ja)
Inventor
根本　直人
直人 根本
山口　淳一
淳一 山口
Original Assignee
有限会社ジーン・フィールド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 有限会社ジーン・フィールド filed Critical 有限会社ジーン・フィールド
Publication of JPWO2005001086A1 publication Critical patent/JPWO2005001086A1/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2005511126A 2003-06-27 2004-06-25 固定化ｍＲＮＡ−ピューロマイシン連結体及びその用途 Pending JPWO2005001086A1 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2003185531 2003-06-27
JP2003185531 2003-06-27
PCT/JP2004/009396 WO2005001086A1 (fr) 2003-06-27 2004-06-25 Conjugue arnm-puromicyne immobilise et utilisation dudit conjugue

Publications (1)

Publication Number Publication Date
JPWO2005001086A1 true JPWO2005001086A1 (ja) 2006-08-10

Family

ID=33549668

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2005511126A Pending JPWO2005001086A1 (ja) 2003-06-27 2004-06-25 固定化ｍＲＮＡ−ピューロマイシン連結体及びその用途

Country Status (2)

Country Link
JP (1) JPWO2005001086A1 (fr)
WO (1) WO2005001086A1 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1816192B1 (fr) * 2004-10-15 2012-12-12 National Institute of Advanced Industrial Science and Technology Lieur de construction de conjugue arnm-puromycine-proteine
GB0511717D0 (en) * 2005-06-09 2005-07-13 Babraham Inst Repeatable protein arrays
JPWO2007046520A1 (ja) * 2005-10-18 2009-04-23 独立行政法人産業技術総合研究所 固定化ピューロマイシン・リンカーを用いたタンパク質のスクリーニング方法
EP2388312A1 (fr) 2010-05-17 2011-11-23 Curetis AG Tampon de lyse applicable universellement et procédés de traitement pour la lyse d'échantillons corporels
JPWO2013073706A1 (ja) * 2011-11-18 2015-04-02 株式会社ニコン スリーフィンガー構造を有するリガンド、及びそれを用いた分子の検出方法
JP7416715B2 (ja) 2018-11-07 2024-01-17 公益財団法人川崎市産業振興財団 ペプチド－核酸複合体

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001348398A (ja) * 2000-03-10 2001-12-18 Japan Science & Technology Corp ５−ピリミジン含有核酸、それを用いた可逆的連結方法
JP2004097213A (ja) * 2002-07-19 2004-04-02 Mitsubishi Chemicals Corp 核酸および／またはタンパク質の選択方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003299489A (ja) * 2002-02-08 2003-10-21 Mitsubishi Chemicals Corp 核酸構築物

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001348398A (ja) * 2000-03-10 2001-12-18 Japan Science & Technology Corp ５−ピリミジン含有核酸、それを用いた可逆的連結方法
JP2004097213A (ja) * 2002-07-19 2004-04-02 Mitsubishi Chemicals Corp 核酸および／またはタンパク質の選択方法

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
JPN6010000722, Ｐｒｏｔｅｏｍｉｃｓ， ｖｏｌ．２， ｐ．４８−５７ （２００２） *
JPN6010000725, ＦＥＢＳ Ｌｅｔｔ．， ｖｏｌ．５０８， ｐ．３０９−３１２ （２００１） *
JPN6010000730, Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓ．， ｖｏｌ．３７， ｅ．１０８ （２００９） *
JPN6010000733, Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓ．， ｖｏｌ．３４， ｅ．１４０ （２００６） *
JPN6010000735, 蛋白質核酸酵素， ｖｏｌ．４９， ｐ．２７６８−２７７４ （２００４） *
JPN6010000737, Ｂｉｏｌ． Ｐｒｏｃｅｄ． Ｏｎｌｉｎｅ， ｖｏｌ．４， ｐ．４９−５４， （２００２） *
JPN6010000739, ＦＥＢＳ Ｌｅｔｔ．， ｖｏｌ．４１４， ｐ．４０５−４０８， （１９９７） *
JPN6010000740, ＰＮＡＳ， ｖｏｌ．９８， ｐ．３７５０−３７５５ （２００１） *
JPN6010000742, Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓ．， ｖｏｌ．３１， ｅ．７８ （２００３） *

Also Published As

Publication number Publication date
WO2005001086A1 (fr) 2005-01-06

Similar Documents

Publication Publication Date Title
EP1816192B1 (fr) Lieur de construction de conjugue arnm-puromycine-proteine
US10088474B2 (en) Methods and compositions for phototransfer
US9334530B2 (en) Methods for making and imaging arrays that comprise a plurality of different biomolecules
US7150978B2 (en) Recombinant template used for producing a carboxy-terminal modified protien and a method of producing a carboxy-terminal modified protein
US8486634B2 (en) Amplifying bisulfite-treated template
US20090270278A1 (en) Methods and compounds for making arrays
US20100075374A1 (en) Methods for capturing nascent proteins
US20090264298A1 (en) Methods for enriching subpopulations
JP5733784B2 (ja) ｃＤＮＡ／ｍＲＮＡ−タンパク質連結体の効率的合成法
US8481263B2 (en) Bead-ligand-nascent protein complexes
JPWO2005001086A1 (ja) 固定化ｍＲＮＡ−ピューロマイシン連結体及びその用途
JP2002253240A (ja) 分子間の相互作用の分析方法
US8932879B2 (en) Methods and compounds for phototransfer
JPWO2007046520A1 (ja) 固定化ピューロマイシン・リンカーを用いたタンパク質のスクリーニング方法
JPWO2003048363A1 (ja) 対応付け分子とｃ末端ラベル化蛋白質の複合体および対応付け分子の複合体、ならびにそれらの複合体を利用した蛋白質間相互作用解析方法
US20070184440A1 (en) Methods of screening for useful proteins
JP4747292B2 (ja) 翻訳テンプレートおよびそのライブラリー、それらから合成される蛋白質および蛋白質のライブラリー、ならびにそれらを構成する要素、ならびにそれらの製造法および利用方法
JP2002257832A (ja) タンパク質のラベル化試薬

Legal Events

Date Code Title Description
A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20070115

A521 Written amendment

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20071228

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20071228

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7423

Effective date: 20080306

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20100112

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20100518