JPS6447381A - Preparation of liposome containing sealed gene - Google Patents

Preparation of liposome containing sealed gene

Info

Publication number
JPS6447381A
JPS6447381A JP20418387A JP20418387A JPS6447381A JP S6447381 A JPS6447381 A JP S6447381A JP 20418387 A JP20418387 A JP 20418387A JP 20418387 A JP20418387 A JP 20418387A JP S6447381 A JPS6447381 A JP S6447381A
Authority
JP
Japan
Prior art keywords
gene
liposome
dna
preparation
reverse
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP20418387A
Other languages
Japanese (ja)
Other versions
JP2627899B2 (en
Inventor
Kunio Yagi
Nakao Kojima
Nobuyuki Haga
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BITAMIN KENKYUSHO KK
VITAMIN KENKYUSHO KK
Original Assignee
BITAMIN KENKYUSHO KK
VITAMIN KENKYUSHO KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BITAMIN KENKYUSHO KK, VITAMIN KENKYUSHO KK filed Critical BITAMIN KENKYUSHO KK
Priority to JP20418387A priority Critical patent/JP2627899B2/en
Publication of JPS6447381A publication Critical patent/JPS6447381A/en
Application granted granted Critical
Publication of JP2627899B2 publication Critical patent/JP2627899B2/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A61K9/1277—Processes for preparing; Proliposomes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Epidemiology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Organic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)

Abstract

PURPOSE:To enable removal of microbial cells with a 0.45mum filter, by adding a polyhydric alcohol at a latter stage of a gene-inclusion process using a reverse- phase evaporation method, thereby efficiently including a DNA in a liposome having a diameter of <=450nm. CONSTITUTION:In the production of a gene-including liposome by a reverse- phase evaporation method, a polyhydric alcohol such as glycerol or ethylene glycol is added at the latter stage of the gene-inclusion process. The liposome suspension obtained by the above process is treated with a Nuclepore filter having an average pore size of 400nm to obtain liposomes composed mainly of those having diameter of 100-400nm. The gene to be included by this process may be a DNA or a recombinant DNA.
JP20418387A 1987-08-19 1987-08-19 Production method of gene-encapsulated liposome Expired - Lifetime JP2627899B2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP20418387A JP2627899B2 (en) 1987-08-19 1987-08-19 Production method of gene-encapsulated liposome

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP20418387A JP2627899B2 (en) 1987-08-19 1987-08-19 Production method of gene-encapsulated liposome

Publications (2)

Publication Number Publication Date
JPS6447381A true JPS6447381A (en) 1989-02-21
JP2627899B2 JP2627899B2 (en) 1997-07-09

Family

ID=16486211

Family Applications (1)

Application Number Title Priority Date Filing Date
JP20418387A Expired - Lifetime JP2627899B2 (en) 1987-08-19 1987-08-19 Production method of gene-encapsulated liposome

Country Status (1)

Country Link
JP (1) JP2627899B2 (en)

Cited By (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5589466A (en) * 1989-03-21 1996-12-31 Vical Incorporated Induction of a protective immune response in a mammal by injecting a DNA sequence
US5693622A (en) * 1989-03-21 1997-12-02 Vical Incorporated Expression of exogenous polynucleotide sequences cardiac muscle of a mammal
JP2001502299A (en) * 1996-09-13 2001-02-20 リポゼン リミテッド Liposome
US6214804B1 (en) 1989-03-21 2001-04-10 Vical Incorporated Induction of a protective immune response in a mammal by injecting a DNA sequence
US6228844B1 (en) 1991-11-12 2001-05-08 Vical Incorporated Stimulating vascular growth by administration of DNA sequences encoding VEGF
US6413942B1 (en) 1989-03-21 2002-07-02 Vical, Inc. Methods of delivering a physiologically active polypeptide to a mammal
WO2002079447A2 (en) 2001-03-30 2002-10-10 Avigenics, Inc. Avian lysozyme promoter
US6706694B1 (en) 1990-03-21 2004-03-16 Vical Incorporated Expression of exogenous polynucleotide sequences in a vertebrate
WO2004064750A2 (en) 2003-01-22 2004-08-05 Duke University Improved constructs for expressing lysosomal polypeptides
US6867195B1 (en) 1989-03-21 2005-03-15 Vical Incorporated Lipid-mediated polynucleotide administration to reduce likelihood of subject's becoming infected
WO2006017673A2 (en) 2004-08-03 2006-02-16 Biogen Idec Ma Inc. Taj in neuronal function
WO2006081331A2 (en) 2005-01-25 2006-08-03 Prolexys Pharmaceuticals, Inc. Quinoxaline derivatives as antitumor agents
US7384923B2 (en) 1999-05-14 2008-06-10 Lipoxen Technologies Limited Liposomes
EP1997829A1 (en) 2001-12-21 2008-12-03 Human Genome Sciences, Inc. Albumin fusion proteins
EP2206720A1 (en) 2000-04-12 2010-07-14 Human Genome Sciences, Inc. Albumin fusion proteins
WO2010096388A2 (en) 2009-02-18 2010-08-26 Carnegie Mellon University Quenched dendrimeric dyes for bright detection
EP2314692A2 (en) 2003-06-02 2011-04-27 University of Massachusetts Methods and compositions for enhancing the efficacy and specificity of RNAi
EP2345720A2 (en) 2001-07-12 2011-07-20 University of Massachusetts In vivo production of small interfering RNAs that mediate gene silencing
WO2011150079A1 (en) 2010-05-25 2011-12-01 Carnegie Mellon University Targeted probes of cellular physiology
EP2441474A1 (en) 2003-10-17 2012-04-18 Joslin Diabetes Center, Inc. Methods and compositions for modulating adipocyte function
EP2629094A1 (en) 2007-01-24 2013-08-21 Carnegie Mellon University Optical biosensors
EP2769732A1 (en) 2013-02-22 2014-08-27 Sanofi Serpins: methods of therapeutic beta-cell regeneration and function
WO2014128257A1 (en) 2013-02-22 2014-08-28 Sanofi Serpins: methods of therapeutic beta-cell regeneration and function
EP2851086A1 (en) 2013-09-20 2015-03-25 Sanofi Serpins: methods of therapeutic ß-cell regeneration and function
WO2017147128A1 (en) 2016-02-22 2017-08-31 The University Of North Carolina At Chapel Hill Peptide inhibitors of calcium channels
US10434177B2 (en) 2014-11-17 2019-10-08 Carnegie Mellon University Activatable two-component photosensitizers

Cited By (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6673776B1 (en) 1989-03-21 2004-01-06 Vical Incorporated Expression of exogenous polynucleotide sequences in a vertebrate, mammal, fish, bird or human
US6413942B1 (en) 1989-03-21 2002-07-02 Vical, Inc. Methods of delivering a physiologically active polypeptide to a mammal
US5703055A (en) * 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US6867195B1 (en) 1989-03-21 2005-03-15 Vical Incorporated Lipid-mediated polynucleotide administration to reduce likelihood of subject's becoming infected
US6214804B1 (en) 1989-03-21 2001-04-10 Vical Incorporated Induction of a protective immune response in a mammal by injecting a DNA sequence
US6710035B2 (en) 1989-03-21 2004-03-23 Vical Incorporated Generation of an immune response to a pathogen
US5693622A (en) * 1989-03-21 1997-12-02 Vical Incorporated Expression of exogenous polynucleotide sequences cardiac muscle of a mammal
US5589466A (en) * 1989-03-21 1996-12-31 Vical Incorporated Induction of a protective immune response in a mammal by injecting a DNA sequence
US6706694B1 (en) 1990-03-21 2004-03-16 Vical Incorporated Expression of exogenous polynucleotide sequences in a vertebrate
US6228844B1 (en) 1991-11-12 2001-05-08 Vical Incorporated Stimulating vascular growth by administration of DNA sequences encoding VEGF
EP1254657A2 (en) * 1996-09-13 2002-11-06 Lipoxen Technologies Limited Liposomes
US7790696B2 (en) 1996-09-13 2010-09-07 Lipoxen Technologies Limited Cationic liposomes containing immune response generating moieties
JP2001502299A (en) * 1996-09-13 2001-02-20 リポゼン リミテッド Liposome
US7384923B2 (en) 1999-05-14 2008-06-10 Lipoxen Technologies Limited Liposomes
EP2206720A1 (en) 2000-04-12 2010-07-14 Human Genome Sciences, Inc. Albumin fusion proteins
EP2357008A1 (en) 2000-04-12 2011-08-17 Human Genome Sciences, Inc. Albumin fusion proteins
EP2311872A1 (en) 2000-04-12 2011-04-20 Human Genome Sciences, Inc. Albumin fusion proteins
EP2295456A1 (en) 2000-04-12 2011-03-16 Human Genome Sciences, Inc. Albumin fusion proteins
EP2213743A1 (en) 2000-04-12 2010-08-04 Human Genome Sciences, Inc. Albumin fusion proteins
EP2216409A1 (en) 2000-04-12 2010-08-11 Human Genome Sciences, Inc. Albumin fusion proteins
EP2298355A2 (en) 2000-04-12 2011-03-23 Human Genome Sciences, Inc. Albumin fusion proteins
EP2236152A1 (en) 2000-04-12 2010-10-06 Human Genome Sciences, Inc. Albumin fusion proteins
EP2267026A1 (en) 2000-04-12 2010-12-29 Human Genome Sciences, Inc. Albumin fusion proteins
EP2275557A1 (en) 2000-04-12 2011-01-19 Human Genome Sciences, Inc. Albumin fusion proteins
WO2002079447A2 (en) 2001-03-30 2002-10-10 Avigenics, Inc. Avian lysozyme promoter
EP2345720A2 (en) 2001-07-12 2011-07-20 University of Massachusetts In vivo production of small interfering RNAs that mediate gene silencing
EP2360251A2 (en) 2001-07-12 2011-08-24 University of Massachusetts In vivo production of small interfering RNAs that mediate gene silencing
EP2261250A1 (en) 2001-12-21 2010-12-15 Human Genome Sciences, Inc. Albumin fusion proteins
EP2277888A2 (en) 2001-12-21 2011-01-26 Human Genome Sciences, Inc. Fusion proteins of albumin and erythropoietin
EP2277910A1 (en) 2001-12-21 2011-01-26 Human Genome Sciences, Inc. Albumin fusion proteins
EP1997829A1 (en) 2001-12-21 2008-12-03 Human Genome Sciences, Inc. Albumin fusion proteins
EP2277889A2 (en) 2001-12-21 2011-01-26 Human Genome Sciences, Inc. Fusion proteins of albumin and interferon beta
WO2004064750A2 (en) 2003-01-22 2004-08-05 Duke University Improved constructs for expressing lysosomal polypeptides
US9873868B2 (en) 2003-01-22 2018-01-23 Duke University Constructs for expressing lysosomal polypeptides
EP2314692A2 (en) 2003-06-02 2011-04-27 University of Massachusetts Methods and compositions for enhancing the efficacy and specificity of RNAi
EP2441474A1 (en) 2003-10-17 2012-04-18 Joslin Diabetes Center, Inc. Methods and compositions for modulating adipocyte function
EP2329714A1 (en) 2004-08-03 2011-06-08 Biogen Idec MA Inc. Influence of TAJ in the neuronal functions
WO2006017673A2 (en) 2004-08-03 2006-02-16 Biogen Idec Ma Inc. Taj in neuronal function
WO2006081331A2 (en) 2005-01-25 2006-08-03 Prolexys Pharmaceuticals, Inc. Quinoxaline derivatives as antitumor agents
EP2629094A1 (en) 2007-01-24 2013-08-21 Carnegie Mellon University Optical biosensors
WO2010096388A2 (en) 2009-02-18 2010-08-26 Carnegie Mellon University Quenched dendrimeric dyes for bright detection
WO2011150079A1 (en) 2010-05-25 2011-12-01 Carnegie Mellon University Targeted probes of cellular physiology
US9995679B2 (en) 2010-05-25 2018-06-12 Carnegie Mellon University Targeted probes of cellular physiology
WO2014128257A1 (en) 2013-02-22 2014-08-28 Sanofi Serpins: methods of therapeutic beta-cell regeneration and function
EP2769732A1 (en) 2013-02-22 2014-08-27 Sanofi Serpins: methods of therapeutic beta-cell regeneration and function
EP2851086A1 (en) 2013-09-20 2015-03-25 Sanofi Serpins: methods of therapeutic ß-cell regeneration and function
US10434177B2 (en) 2014-11-17 2019-10-08 Carnegie Mellon University Activatable two-component photosensitizers
US10946098B2 (en) 2014-11-17 2021-03-16 Carnegie Mellon University Activatable two-component photosensitizers
WO2017147128A1 (en) 2016-02-22 2017-08-31 The University Of North Carolina At Chapel Hill Peptide inhibitors of calcium channels

Also Published As

Publication number Publication date
JP2627899B2 (en) 1997-07-09

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