JPS5794292A - Culturing of lymphoidoblast cell - Google Patents
Culturing of lymphoidoblast cellInfo
- Publication number
- JPS5794292A JPS5794292A JP55169261A JP16926180A JPS5794292A JP S5794292 A JPS5794292 A JP S5794292A JP 55169261 A JP55169261 A JP 55169261A JP 16926180 A JP16926180 A JP 16926180A JP S5794292 A JPS5794292 A JP S5794292A
- Authority
- JP
- Japan
- Prior art keywords
- cultivation
- medium
- cells
- lymphoidoblast
- extracting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
PURPOSE: To increase the production of an objective substance remarkably in the cultivation of lymphoidoblast cells capable of continuously producing the objective substance, by extracting the filtrate of the cultivation medium during cultivation, and continuing the cultivation while replenishing the medium with fresh one.
CONSTITUTION: A cultivation tank (pref. spin filter cultivation tank) is charged with a medium for the cultivation of mammalian cells (e.g. Eagle's MEM medium containing about 0.1W15v/v% blood serum of bovine fetus). Cells (capable of continuously producing interferon, e.g. human lymphoidoblast cells transformed with Epstein-Barr Virus) are added to the medium in concentration of about 105W107 cells/ml, and subjected to the aerobic cultivation. The cultivation is continued by extracting a half to the whole quantity of the cultivation medium about every 0.5W2 days, and adding the same amount of fresh medium.
COPYRIGHT: (C)1982,JPO&Japio
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55169261A JPS5794292A (en) | 1980-12-01 | 1980-12-01 | Culturing of lymphoidoblast cell |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP55169261A JPS5794292A (en) | 1980-12-01 | 1980-12-01 | Culturing of lymphoidoblast cell |
Publications (2)
Publication Number | Publication Date |
---|---|
JPS5794292A true JPS5794292A (en) | 1982-06-11 |
JPS6232918B2 JPS6232918B2 (en) | 1987-07-17 |
Family
ID=15883222
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP55169261A Granted JPS5794292A (en) | 1980-12-01 | 1980-12-01 | Culturing of lymphoidoblast cell |
Country Status (1)
Country | Link |
---|---|
JP (1) | JPS5794292A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6163284A (en) * | 1984-09-05 | 1986-04-01 | Teijin Ltd | Cultivation of animal cells |
-
1980
- 1980-12-01 JP JP55169261A patent/JPS5794292A/en active Granted
Non-Patent Citations (1)
Title |
---|
JGEN VIROL=1973 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS6163284A (en) * | 1984-09-05 | 1986-04-01 | Teijin Ltd | Cultivation of animal cells |
Also Published As
Publication number | Publication date |
---|---|
JPS6232918B2 (en) | 1987-07-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JPS54147997A (en) | Novel hyaluronidase bmp-8231 and its preparation | |
SE8201554L (en) | SET TO MAKE SUBSTANCES WHICH CREATE CELLS | |
ES465059A1 (en) | Production of specific immune nucleic acids cell dialysates and antibodies | |
ATE75775T1 (en) | PROCESS FOR PRODUCTION OF BIOLOGICALLY ACTIVE PLASMINOGEN ACTIVATOR. | |
ES8403524A1 (en) | Yeast culture containing S-adenosyl methionine in high concentrations, and process for production of S-adenosyl methionine | |
JPS5794293A (en) | Preparation of urokinase | |
JPS5639779A (en) | Cell culturing method of hematisis a type virus | |
JPS5765178A (en) | Plant tissue or cell culture | |
Pilwat et al. | Immobilization of human red blood cells | |
JPS5754586A (en) | Cell strain producing human interferon prolongably, its preparation, and preparation of human interferon using it | |
JPS5794292A (en) | Culturing of lymphoidoblast cell | |
SE8603711L (en) | METHOD OF PRODUCING HUMANA MONOCLONAL ANTIBODIES | |
AU5009679A (en) | Purified serum factors in animal cell culture medium | |
JPS57115195A (en) | Preparation of human calcitonin | |
JPS5765179A (en) | Cell culture | |
JPS5716694A (en) | Fixing method of cell | |
JPS5668388A (en) | Production of cell body of spirulina algae | |
DE69116240D1 (en) | Process for the production of cell protein with anti-HIV activity | |
JPS527478A (en) | Cell incubator | |
Maquart et al. | A purified structural glycoprotein determines a strong inhibition of protein synthesis in fibroblast cultures | |
JPS5774093A (en) | Method of increasing interferon production | |
ES8106758A1 (en) | Preparation of 2,5-diketogluconic acid | |
DK0485504T3 (en) | Process for Preparation of Activated Protein C by Cell Culture | |
JPS57186491A (en) | Cultivation of fibroblast using fibronectin | |
Conway et al. | Localization of electrolytes in muscle |