JPS6398330A - Culture medium for strand of amanori - Google Patents
Culture medium for strand of amanoriInfo
- Publication number
- JPS6398330A JPS6398330A JP24421786A JP24421786A JPS6398330A JP S6398330 A JPS6398330 A JP S6398330A JP 24421786 A JP24421786 A JP 24421786A JP 24421786 A JP24421786 A JP 24421786A JP S6398330 A JPS6398330 A JP S6398330A
- Authority
- JP
- Japan
- Prior art keywords
- culture medium
- parts
- weight
- calcium carbonate
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000001963 growth medium Substances 0.000 title claims description 17
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 24
- 239000000843 powder Substances 0.000 claims description 14
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 12
- 238000005245 sintering Methods 0.000 claims description 8
- 241000206607 Porphyra umbilicalis Species 0.000 claims description 2
- 239000000203 mixture Substances 0.000 description 7
- 239000008187 granular material Substances 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000000465 moulding Methods 0.000 description 4
- 241000237502 Ostreidae Species 0.000 description 3
- 235000020636 oyster Nutrition 0.000 description 3
- 235000015170 shellfish Nutrition 0.000 description 3
- 239000005995 Aluminium silicate Substances 0.000 description 2
- 235000019738 Limestone Nutrition 0.000 description 2
- 235000012211 aluminium silicate Nutrition 0.000 description 2
- 239000011230 binding agent Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 239000003365 glass fiber Substances 0.000 description 2
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 description 2
- 239000006028 limestone Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 241001474374 Blennius Species 0.000 description 1
- 229910021532 Calcite Inorganic materials 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000237503 Pectinidae Species 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229940043430 calcium compound Drugs 0.000 description 1
- 150000001674 calcium compounds Chemical class 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 210000001268 chyle Anatomy 0.000 description 1
- 239000004927 clay Substances 0.000 description 1
- 239000011162 core material Substances 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 210000003278 egg shell Anatomy 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 235000020637 scallop Nutrition 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Landscapes
- Cultivation Of Seaweed (AREA)
Abstract
(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Abstract] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】 本発明はアマノリ類糸状体の培養基に関する。[Detailed description of the invention] TECHNICAL FIELD The present invention relates to a culture medium for porphyry filaments.
従来、アマノリ類の糸状体の培養生育には、カキ貝、ホ
タテ貝等の貝殻類が用いられ、最近ではプラスチック、
金属、セラミックス等の基板上に、接着剤や結合剤を利
用して貝殻類粉粒体、カルシウム成分を含有する鉱物粉
粒体又は人工のカルシウム化合物粉粒体を接着又は押圧
埋入し又はこの粉粒体からなる層を設けた培養基も考案
されている（例えば特開昭５２−２４８９４号、実開昭
５０−９４１９６号公報等）。Conventionally, shellfish such as oyster shells and scallops have been used to culture and grow the filamentous bodies of seaweed, but recently plastics,
Shellfish powder, mineral powder containing calcium components, or artificial calcium compound powder is bonded or pressed onto a substrate made of metal, ceramics, etc. using an adhesive or a binder, or Culture media provided with a layer made of powder have also been devised (for example, Japanese Patent Application Laid-open No. 52-24894, Japanese Utility Model Application No. 50-94196, etc.).
しかし貝殻類では異臭を放つこと、形状が不揃いで取扱
いが不便であること、凹凸があるため均一性に欠けるこ
と、更に近時では入手も困難になりつつあること、また
後者の人工品でも粉粒体が作業中に脱落し、また粉粒体
が各個独立しているため、糸状体の生育が粉粒体を伝わ
りにく（、単位面積当りの培養密度が低い等の欠点があ
る。そこで糸状体の生育に適したバインダーを用いた培
養基が考案されているが（例えば特公昭５７−５４９０
号公報、実公昭５６−１２７１４号公報等）、経済性に
問題がある。However, shellfish emit a strange odor, are inconvenient to handle due to irregular shapes, lack uniformity due to unevenness, and are becoming difficult to obtain these days. Because the granules fall off during work, and because each granule is independent, the growth of filaments is difficult to propagate through the granules (there are drawbacks such as low culture density per unit area. Culture media using binders suitable for the growth of filamentous bodies have been devised (for example, in Japanese Patent Publication No. 57-5490).
(Japanese Utility Model Publication No. 56-12714, etc.), there are problems in economic efficiency.
本発明はこのような欠点を除いたもので、炭酸カルシウ
ムを主成分とする粉粒体の成形物を焼結して成るアマノ
リ類糸状体培養基である。The present invention eliminates these drawbacks and is a culture medium for filaments of laver, which is obtained by sintering a molded product of powder or granules containing calcium carbonate as a main component.
本発明に用いられる炭酸カルシウムを主成分とする粉粒
体としては、合成炭酸カルシウム、人工炭酸カルシウム
、石灰石、大埋石、方解石、貝殻、卵殻等の粉粒体、並
びにこれらの粉粒体と焼結性、成形性、強度、経済性を
向上させるための材料、あるいは増量材例えば粘土、カ
オリン、砂等の混合物が用いられる。粉粒体の粒度は６
００メツシユ以下が好ましい。成形物の強度を高めるた
め、ガラス繊維、金属心棒、金網等を心材として用いる
こともできる。The granules containing calcium carbonate as a main component used in the present invention include powders such as synthetic calcium carbonate, artificial calcium carbonate, limestone, large stone, calcite, seashells, and eggshells, as well as powders and granules of these materials. Mixtures of materials or fillers such as clay, kaolin, sand, etc. are used to improve sinterability, formability, strength, and economic efficiency. The particle size of the powder is 6
00 mesh or less is preferable. In order to increase the strength of the molded product, glass fiber, metal mandrel, wire mesh, etc. can also be used as the core material.
本発明の培養基を製造するに際しては、炭酸カルシウム
を主成分とする粉粒体を任意の形状例えば板状、円柱状
、角柱状等に成形する。成形法としては加圧成形、押出
し成形、流し込み成形等の方法が用いられる。When producing the culture medium of the present invention, powder particles containing calcium carbonate as a main component are formed into any desired shape, such as a plate, cylinder, or prism. As the molding method, methods such as pressure molding, extrusion molding, and casting molding are used.
次いで成形物を焼結すると目的の培養基が得られる。焼
結温度は４００〜１６００°Ｃ％に８００〜９００℃が
好ましい。焼結時間は焼結温度によって異なるが、通常
は１〜５時間程度である。成形物中の炭酸カルシウムの
分解を防ぐため、成形物の焼結は炭酸ガス雰囲気、高圧
雰囲気等で行われる。成形と焼結を兼ねたホットプレス
法を用いろこともできる。The molded product is then sintered to obtain the desired culture medium. The sintering temperature is preferably 400-1600°C%, preferably 800-900°C. The sintering time varies depending on the sintering temperature, but is usually about 1 to 5 hours. In order to prevent the decomposition of calcium carbonate in the molded product, the molded product is sintered in a carbon dioxide gas atmosphere, high pressure atmosphere, etc. It is also possible to use a hot press method that combines molding and sintering.
実施例１
軽質炭酸カルシウム８０重量部及び石灰石粉末（６００
メツシユ）２０重量部を均質になるようによく混和し、
これに水１０重量部を加えて加温し、この加湿粉末１５
ｇを３００ ｋｇ／ ｃｍ２で加圧成形し、直径５５ｎ
ｍ、厚さ３ ｍｍの円板を得た。この円板を水分が０．
６％以下になるまで乾燥させたのち、焼結炉（電気炉）
に入れ、常圧下で炉内を１００％炭酸ガス雰囲気にして
毎分２０°○の割合で昇温し、８５０℃に２時間保持し
て焼結したのち、自然冷却すると、目的の培養基が得ら
れた。Example 1 80 parts by weight of light calcium carbonate and limestone powder (600 parts by weight)
Mix 20 parts by weight of mesh powder thoroughly until homogeneous,
Add 10 parts by weight of water to this, heat it, and make 15 parts of humidifying powder.
Pressure molded with 300 kg/cm2 to form a diameter of 55n.
A disk with a thickness of 3 mm was obtained. The water content of this disk is 0.
After drying to below 6%, sintering furnace (electric furnace)
The culture medium was placed in a 100% carbon dioxide atmosphere under normal pressure, heated at a rate of 20° per minute, held at 850°C for 2 hours for sintering, and then allowed to cool naturally to obtain the desired culture medium. It was done.
実施例２
軽質炭酸カルシウム１００重量部に水１０重量部を加え
て加湿し、以下実施例１と同様にして目的の培養基を製
造した。Example 2 10 parts by weight of water was added to 100 parts by weight of light calcium carbonate and humidified, and the same procedure as in Example 1 was repeated to produce the desired culture medium.
実施例３
軽質炭酸カルシウム８０重量部及び貝殻粉末（２５０メ
ツシユ）２０重量部を均質に混合し、これに水１０重量
部を加えて加湿し、以下実施例１と同様にして目的の培
養基を製造した。Example 3 80 parts by weight of light calcium carbonate and 20 parts by weight of seashell powder (250 mesh) were mixed homogeneously, 10 parts by weight of water was added to the mixture and humidified, and the desired culture medium was produced in the same manner as in Example 1. did.
実施例４
軽質炭酸カルシウム９７重量部及びカオリン６重量部を
均質になるようによく混和し、これに水１０重量部を加
えて加湿し、以下実施例１と同様にして目的の培養基を
製造した。Example 4 97 parts by weight of light calcium carbonate and 6 parts by weight of kaolin were thoroughly mixed to make it homogeneous, 10 parts by weight of water was added to the mixture, and the mixture was humidified, and the desired culture medium was produced in the same manner as in Example 1. .
実施例５
軽質炭酸カルシウム９７重量部及び長さ１０朋のガラス
繊維３重量部をよく混和し、これに水１０重量部を加え
て加湿し、以下実施例１と同様にして目的の培養基を製
造した。Example 5 97 parts by weight of light calcium carbonate and 3 parts by weight of glass fibers having a length of 10 mm were thoroughly mixed, 10 parts by weight of water was added to the mixture, and the mixture was humidified. The desired culture medium was then produced in the same manner as in Example 1. did.
試験例
実施例１〜５により得られた焼結体培養基及びカキ貝を
用い、糸状体培養試験を行った。これらの培養基にアマ
ノリ胞子を種付し、海水中で６力月培養し、糸状体の生
育状況及び殻胞子の発生状況を調査した。その結果を下
記表に示す。この成績により、本発明の培養基はカキ殻
と同様に糸状体の生育に適していることが知られる。評
価の尺度は、＋はやや効果あり、−１−＋−ｔ＋＋−は
優良を意味する。Test Example Using the sintered body culture medium and oyster shells obtained in Examples 1 to 5, a filament culture test was conducted. Noriflora spores were seeded on these culture media and cultured in seawater for 6 months, and the growth status of filaments and the occurrence of chyle spores were investigated. The results are shown in the table below. Based on this result, it is known that the culture medium of the present invention is suitable for growing filamentous bodies as well as oyster shells. As for the evaluation scale, + means somewhat effective and -1-+-t++- means excellent.
Claims (1)
て成るアマノリ類糸状体培養基。A laver filament culture medium made by sintering a powder molded product containing calcium carbonate as a main component.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24421786A JPS6398330A (en) | 1986-10-16 | 1986-10-16 | Culture medium for strand of amanori |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP24421786A JPS6398330A (en) | 1986-10-16 | 1986-10-16 | Culture medium for strand of amanori |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6398330A true JPS6398330A (en) | 1988-04-28 |
| JPH0251571B2 JPH0251571B2 (en) | 1990-11-07 |
Family
ID=17115486
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP24421786A Granted JPS6398330A (en) | 1986-10-16 | 1986-10-16 | Culture medium for strand of amanori |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6398330A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5389154A (en) * | 1992-06-23 | 1995-02-14 | Nippon Telegraph And Telephone | Plasma processing apparatus |
| US6132550A (en) * | 1995-08-11 | 2000-10-17 | Sumitomo Electric Industries, Ltd. | Apparatuses for desposition or etching |
-
1986
- 1986-10-16 JP JP24421786A patent/JPS6398330A/en active Granted
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5389154A (en) * | 1992-06-23 | 1995-02-14 | Nippon Telegraph And Telephone | Plasma processing apparatus |
| US6132550A (en) * | 1995-08-11 | 2000-10-17 | Sumitomo Electric Industries, Ltd. | Apparatuses for desposition or etching |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0251571B2 (en) | 1990-11-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EXPY | Cancellation because of completion of term |