JPS63500981A - Method for producing protein-vitamin concentrates - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] Method for producing protein-vitamin concentrates Technical field The present invention relates to microbial industry, and more specifically, the present invention relates to microbial industry, and more specifically, Also useful as an additive in the microbial industry, pharmaceutical industry, cosmetics industry, and other industries. (1) It can be used as a source of amino acids, various fats, and a source of vitamins. Method of producing a protein-vitamin concentrate suitable for use:

Furthermore, the method according to the invention enables the production of It can be used for purifying wastewater.

Background technology Synthesis involving carbohydrates or hydrocarbons! #, yeast under aerobic conditions on nutrient medium Various methods of producing protein-vitamin concentrates by enriching Technically known [Biochikunoronoh of the Micronoiful Synthesizer] Swiss, Riga, ``Nona 1 Ne'' I (BiOtechnolo gy of the Microbial 5ynthesis+RiBm, “ Zinatne@Publishers) v 1980t pp, 12 these The preparation of the synthetic Eiga medium that can be used in the method was carried out by Mr. 81 of the Master of Elements and Trace Elements (asLerbaLehes) 111. Their dissolution, carbon source/preparation and Sakae 1! Includes introduction into the culture medium Ru. Carbon sources such as n-furcans are virtually insoluble in the aqueous phase and therefore Not only is it related to the preparation of nutrient media for microorganisms, but also the cultivation method is carried out. Some difficulties have been encountered in connection with the application, especially the emulsification of n-alkanes. Consideration of the reaction medium, such as individual feeding of culture medium and carbon source, forced aeration conditions, etc. This causes problems with agitation. The growth rate of yeast on n-alkanes is faster than on carbohydrates. The mass transfer of QMVCII is 63-3.5 times lower than that of QMVCII. er) properties by the same multiple. Furthermore, n-al Commercial methods of production of biomass (bio@ass) in cans are 0.20- At flow rates higher than 0.2 S h-' (J1! ) is practically impracticable.

These methods also provide low content of no more than 50-60% in protein-vitamin concentrates. It is also characterized by the amount of protein.

Microorganisms were grown under aerobic conditions on a nutrient medium containing a carbon source, black mineral components, and growth stimulants. Protein-vitamin concentrates are produced by culturing yeast with lucwhey. The method of production is also known in five industries (USSR Inventor's Certificate No. 571339, Ink, CI, C12N, 197G).

Wb e Y is made from liquid production waste generated from the manufacture of dairy products. lactose, which serves as a carbon source for the yeast in the above method. Contains wind.

By using whey as a nutrient medium, there is virtually no presence in whey. Additional inorganic salts may be required to be incorporated. Additionally, lactose in whey The gas content is not sufficient and therefore the process requires pre-concentration of the It is necessary to increase the lactose concentration in the whey. whey protein is # Interfering with the strong growth of the mother, the whey proteins are therefore removed in advance. child All of these fabrications are ways to complicate the production of protein-vitamin concentrates. Also, nutrient media based on Hojii are susceptible to foaming even under low intensity aeration. (This ventilation has a detrimental effect not only on process activity but also on production hygiene.) There are drawbacks that can be attributed to various things. Additionally, the rather complex and power-consuming nature of nutrient media The pre-preparation of allows the yeast culture step to be carried out only under discontinuous production conditions, thus reducing the efficiency of yeast biomass production. Obtained protein-vitamin concentrate The protein content in it does not exceed 50-55%.

Disclosure of invention The present invention relates to a method for producing protein-vitamin concentrates, which method is simpler. It not only features simple and less expensive technology, but also wastewater utilization and purification. At the same time, ensure that the features are used! ! achieved by improving the culture medium. Ru.

The above purpose is carried out aerobically on a nutrient medium containing carbon sources, mineral components and growth stimulants. It is possible to cultivate yeast under sexual conditions, then separate the biomass and obtain the desired product. According to the present invention, in a method for producing a protein-vitamin concentrate by , as a nutrient medium, at least 0.01% by mass of lipids, 0.01% by mass of Exhaust from meatpacking factories containing protein components, o, oos mass% carbohydrates water with addition of nitrogen- and phosphorus-containing inorganic salts in an amount of at least 0.1% by volume of the wastewater. It can be achieved by using

Preference is given to using ammonium phosphate as nitrogen- and phosphorus-containing inorganic salt.

In order to increase biomass yield, n7-furcans are added to the nutrient medium. Add in an amount of 0, 1-4% by volume of the medium.

Total bile in an amount of 0.1-0.0005% by volume of the nutrient medium as a yeast growth stimulant It is appropriate to further introduce into the nutrient medium.

The method of the invention is economically effective. from meat industry factories as a nutrient medium. Production waste – wastewater can be utilized and also used for the production of protein – vitamin concentrates. A simplified procedure for construction is possible.

By means of the method of the invention, a protein-vitamin concentrate with a high content of microbiologically valuable It becomes possible to contain certain substances and produce them in high yields. The method of the present invention ensure the utilization and purification of wastewater.

BEST MODE FOR CARRYING OUT THE INVENTION The method for producing the protein-vitamin concentrate is carried out as follows.

0.01% by mass lipid, 0.01% by mass protein component, 0.005% by mass charcoal Wastewater from meatpacking plants containing at least hydrated rs') or effluents with nitrogen- and phosphorus-containing inorganic salts, Add it to the wastewater in an amount of 0.1% or more by volume, introduce the inoculated yeast culture into it, and then Cultivate the selected yeast culture under aeration at the temperature and pH value optimal for the selected yeast culture. .

Preference is given to using ammonium phosphate as nitrogen- and phosphorus-containing inorganic salt.

As the inoculum culture, any yeast culture, such as Cannoda sp. or Satucharomyces genus can be used.

The above culture is carried out by continuous or discontinuous down flow method under aeration. additional carbon source As desired, n-furcans are added to the yeast culture! ! Select bacteria appropriately, It is introduced at a concentration ranging from 0.1-4% by volume of the medium. Whole bile as a growth stimulant is introduced at a concentration of O,O,OO5-0.1% by volume of the medium. Make full use of carbon sources Use # to separate the mother biomass and obtain a protein-vitamin concentrate. protein-bi Tamin concentrate can be used in liquid form or in bast-like form for feeding animals or poultry. It can also be used in dry and dry form, or after suitable processing, as a seed for the food industry. Protein-vitamin concentrate according to the invention which can be used in various sectors The production method has a number of advantages over the prior art. Nutrient medium base, ready Wastewater from meatpacking factories constitutes industrial waste and cannot be used commercially. Ru. In addition to adding a single source of nitrogen and phosphorus to the nutrient medium, additional By adding a carbon source in the form of lucans or a yeast growth stimulant in the form of whole bile, It becomes possible to increase the yield of biomass. Therefore, the method according to the invention In this case, a medium is used that does not require any preliminary processing: it is economically effective, thereby providing high amounts of protein and other biologically valuable It becomes possible to obtain high yields of yeast biomass containing components. yeast growth The process uses organic components from wastewater from meatpacking factories, so this method is recommended. The sequence also makes it possible to ensure the purification of these wastewaters at the same time. Yeast culture conditions considers an unfavorable low pH value for the growth of bacteria micro70-la. Therefore, the use of the method according to the invention reduces bacterial seeding of wastewater discarded after Pil fermentation. will contribute to Effluents from meatpacking plants contain natural high-quality carbon sources. Therefore, the biochemical composition of yeast biomass is different from that of yeast produced by conventional methods. 1 produced by the method of the invention, e.g. The total amount of protein and lipid in the biomass obtained, calculated on dry solids, is 78-80%, whereas those obtained by yeast cultivation on carbohydrates or hydrocarbons The above parameters for biomass collected do not exceed 70-72%. In addition, raw The application of synthetic protein-vitamin preparations is not limited in terms of toxicological requirements. .

For a better understanding of the present invention, a method for producing protein-vitamin concentrate will be explained. Some specific examples are given below.

Example 1 0.1% by mass fat, 0.05% by mass protein, o, oos trade% carbohydrates 0.2% by mass of ammonium phosphate was added to wastewater 11 from a meat factory containing Kannobu sp, (H Add yeast of Candida sp. Under ventilation, inside #2 Temperature of 8-30℃, p+ of 4.0-4.2, discontinuous process conditions and non-sterile conditions Culture is carried out for a period of 3 hours under conditions.

After completing the cultivation, the biomass is separated and dried. ! 2 Dry form of produced protein - Vita The yield of min concentrate is 1.28 g/ffi, and the protein content in it is 69. 3% by mass, and riboflavin is 130.8η.

Example 2 1.5% by mass of fat, 0.3% by mass of protein, 0.007% by mass of carbohydrates 0.2% by mass of ammonium phosphate was added to wastewater IIl from a meatpacking factory. and 0.05% (calculated as dry solids) of Yeast Cantuta sp, Add.

In the fermenter under ventilation: at a temperature of '10-32'C and a pH of 4.2-4.5. The cultivation is carried out discontinuously under non-sterile conditions for a period of 7 hours.

After completing the cultivation, the biomass is separated and dried. Protein in dry form - vitamin concentrate The yield of the condensate is 16.989/4, and the protein content in it is 75.21 mass % and contains riboflavin! is 340.7η/yo.

Example 3 1.0IIRik% lipid, 0.2% protein, o, oos trade mass carbohydrate 0.2% by mass of ammonium phosphate in wastewater from meatpacking factories containing was added, and the yeast Yeast strain sp. Apply to mold and culture.

Cultivation is carried out at a temperature of 30-32°C and a pH of 4.0-4.2. process productivity is 5.83 gels of protein-vitamin concentrate per hour (as dry solids) ), the protein content of which is 74.92% by mass, and the riboflavonate content is 74.92% by mass. The content of the bottle is 321.5η/kg.

Example 4 1. OWI mass% fat, 0.2 mass% protein, 0.012 mass% carbohydrate Wastewater from effluents from meatpacking factories containing 0.1% by mass of phosphoric acid The yeast of Calomyces sp. is subjected to aerobic down-flow culture.

Culture at a temperature of 2B-32°C, a pH of 4.0-4.2 and a flow rate of 0.45 h-'. nourishment. Process productivity is protein-vitamin concentrate (dry form) S per hour , 03y/II.

The protein content is 73.3% by mass, and the riboflavin content is 73.3% by mass. The amount is 312q/kJ! It is.

Example 5 0.09 mass% lipid, 0.09 mass% protein and 0.61 mass% carbohydrate 0.2% by mass of 7 mol of phosphoric acid was added to 1N of wastewater from a meatpacking factory containing Ni Lamb, 0.01['1% total bile and 1s yeast Satucharomyces sp, (7 5% moisture). Under aerobic conditions, no! &i, temperature of 30℃, 4.2 Cultures are carried out in pt-t on shaking for a period of 4 hours. Dry protein - vitamins The yield of concentrate was 5.36y/'J2. and the protein content in it is 71.8 % by weight, and the riboflavin content is 235.6η/~.

Example 6 It is carried out in the same manner as described in Example 5 above.

0.0005% by weight of total bile is introduced into the nutrient medium. Dry protein - vitamins The yield of the concentrate is 4.2 g/l, and the protein content in it is 70.7% by mass. and the riboflavin content is 226.8q/IJ.

Example 7 1.0% fat, 0.08% protein, 0.005% carbohydrates by mass. Sakae IJl culture for yeast culture based on wastewater from meat industry factories containing 1iiiu the ground. The nutrient medium also contained 3.0% by mass of n-furcans ( mixture of CI2-cps fractions) and 0.3% by mass of ammonium sulfate, 0 ．． 08% by weight potassium phosphate (-basic), 0.03% by weight sodium phosphate (dibasic) is also added. 6f mother Kanjig Sρ, 3g/l (dry solid 5 (calculated as r) in a fermenter for 12 hours under aerated conditions, 32-34 C. and a pH value of 4.0-4.2. Dry protein - Vita The yield of min concentrate is 37.8g/4, and the protein content in it is 75.3g/4. and the riboflavin content is 396.31 PF/fin.

Example 8 Repeat the method of Example 7 above. Introducing 0.02% total bile into the nutrient medium. Ru. The yield of dry protein-vitamin concentrate was 40.5 g/It, and the protein content is 74.8% and the riboflavin content is 350. Oq/ji is .

Industrial applicability Protein-vitamin concentrates produced by nature are used in animal and bird breeding. It is possible to apply it as an additive. This concentrate also has microbial Amino acid source, microbial origin, used in industry, pharmaceutical industry and other related industries. A variety of sources of lipids can also serve as a source of vitamins, especially oil-tight soluble vitamins. . In addition, the proposed method can be used for the purification of wastewater from meatpacking plants. I can do it.

International survey report

Claims (4)

[Claims] 1. Yeast were grown under aerobic conditions on a nutrient medium containing carbon sources, mineral components, and growth stimuli. protein by culturing, then separating the biomass and obtaining the desired product. In the method for producing white matter-pitamine concentrate, as a nutrient medium, 0.01 mass % of fat, 0.01% by mass of protein ingredients, 0.005% by mass of carbohydrates wastewater from meat-packing factories containing at least 0.1% by volume of the wastewater; A protein characterized in that it is used by adding a nitrogen- and phosphorus-containing inorganic salt. Method of producing concentrates. 2. characterized by the use of ammonium phosphate as nitrogen- and phosphorus-containing salt A method for producing a protein-pitamine concentrate according to claim 1. 3. Further introducing n-alkanes into the nutrient medium in an amount of 0.1-4% by volume of the medium. The protein-pitamine concentrate according to claims 1-2, characterized in that: production method. 4. As a growth stimulus, in a nutrient medium in an amount of 0.1-0.0005% by volume of the medium. The protein according to claims 1-3, characterized in that whole bile of Quality - Method for producing pitamine concentrate.