SU1392092A1 - Method of producing biomass - Google Patents

Description

(21) 3996304 / 30-13

(22) December 17, 85

(46) 04/30/88. Bul Number 16

(71) Scientific and Production Association of Fat-and-Oil Industry

(72) M.Ya.Savchuk, L.A.Pokotilo, V.N.Krasnpnikov, A.F. Vatsura, M.L.Domoroschenkov and A.G. Peychev

(53) 663.14 (088.8)

(56) USSR Author's Certificate No. 534487, cl. C 12 N 1/16, 1974.

Zabrodsky A.G. Technologists and control of fodder yeast production at molasses bard. M .: Food industry, 1980, p. 73.

(54) METHOD OF OBTAINING BIOMASS

(57) The invention relates to the microbiological industry, to the production of fodder yeast. The aim of the invention is to increase the growth of yeast and improve its quality, as well as reduce the cost of the process. The method consists in that the yeast is grown on a nutrient medium containing distillery stillage and mineral salts, the biomass is separated by stepwise separation and washing from the stillage substances, and the culture fluid is returned to the yeast growing stage. At the same time, the biomass is washed from the bards substances by soy serum, after which it is introduced into the nutrient medium in the after-alcohol bard-short ratio of 20: 1 - 1: 1 (according to), 1 table.

About s

QD

1U

This invention relates to the microbiological process, to the production of fodder yeast.

The purpose of the invention is to increase the yield of yeast and improve their quality, and also reduce the cost of the process.

The method consists in that the soy whey is supplied to the nutrient medium at the stage of washing the yeast suspension from bard substances, when returning the culture fluid to growing biomass. During the washing process the yeast suspension is enriched with the nutrients contained in the soy whey. The cheese is sent to a yeast growth apparatus. This method of supplying whey to the environment saves water and provides maximum use of dry matter — soybean serum, thereby increasing biomass yield, increasing the feed value of e) carrots, saving mineral acids. In the process of washing and separation, non-soluble protein is removed to whey substances that are not acclimated — or difficult to assimilate by yeast — which at the same time increase the feed value of the final product — dry feed yeast by increasing the crude protein content by 3.0–5.1%.

The soy whey filtrate, directed to the yeast growth apparatus, contains additional sources of carbon, minerals, growth factors that increase the yeast yield by 2.5-14%

The acid reaction of the serum allows the pH in the yeast growth apparatus to be maintained in the o-puminal region, thereby reducing the acid consumption for acidification by. 2.8-4;, 8%. Due to the presence of phosphorus compounds in serum, the saving of phosphoric acid is 14.3-28.6%.

When applying the whey directly to the yeast growth apparatus, the biomass yield is lower than when feeding soybean serum separated from insoluble substances, which are not utilized or difficult to produce by yeast. In addition, in this case energy-grates increase, the water consumption and the amount of contaminated wastewater increase.

Pshdeva Soev serum with a solids content of 2.0-5.0% (low

concentrated) is a waste from the production of edible plant protein isolates and concentrates,

Nutrient medium for growing biomass is prepared by adding to the cooled alcohol-containing molasses bard a culture fluid obtained after condensing the biomass by stepwise separation, urea solution (46% nitrogen) is added at the rate of 70 kg and phosphoric acid in the form of phosphoric acid (70%) in the amount of 65 kg per 1 ton of dry yeast. Soyaev serum is introduced into the yeast suspension collection after the 11th separation step in a ratio of 1: 1 to the volume of the suspension. The value of this ratio depends mainly on the content of vinasse in the product - fodder yeast.

Yeast cultivation parameters are as follows:

The dilution rate of the medium, h Temperature, with air consumption per

0.15-0.10 34-38

aeration, h

55-60

pH culture

environments support

by adding

salt or sulfur

acids to the value of 3.5-4.5

The culture medium is continuously withdrawn from the yeast-growth process and, after demulsification, is fed to the biomass distribution by means of stepwise separation and washing it from bards using serum.

The method is illustrated by examples, the results of which are presented in the table.

In the control and experimental variants, an association of cultures of Candida utilis L-35, Torulopsis pinus L-30, and Trichosporon cutaineum L-52 cultures was cultivated on a post-alcohol bard of the same composition and under the same growing conditions:

Temperature, С pH medium Air consumption for aeration,

Growing time, h

34-36 3.6-4.0

55-60

About 8

The difference was in the amount of soy whey supplied. In examples 1 - 3, the ratio of the brew - soyabat despartate serum was 1: 1 - 20: 1, as a result, the yield of yeast increased by 1.0 - 14%, the consumption of phosphoric acid decreased by 1A, 3-28.6%, and the consumption of hydrochloric acid used to maintain the pH of the medium | is 2.3-4.3%. Reduced consumption

water used to wash the phosphoric acid yeast by 14.3%. chewing suspension, was 100 - 5%. With a further decrease in serum consumption (example 4), its effect on the growth

with washing the yeast suspension with whey after stage II

As a result, the increase in the yield of fodder yeast compared with the role was 2.5%, the content of go protein in yeast increased by 3.0%, the ash content decreased by 0, the water consumption decreased by 10%, and

Claims (1)

Formula of the invention. Method of biomass production, p Looking at growing fodder yeast under aeration conditions on a continuous medium containing bard and mineral salts, dividing biomass by stepwise separation and rinsing from bard matter, returning the culture fluid to the yeast growing stage, in order to improve the yield of yeast their qualities, as well as in the process of washing, biome washing yeast imperceptibly. The biomass yield is the same as in the control experiment The use of soy whey instead of water made it possible to improve the quality of fodder yeast, the crude protein content was 1.7-5.1 abs.% Higher than in the control, and the ash content decreased by 0.3 - 1.3%. The cultivation of biomass is carried out in a continuous flow process of mixing. 20 looking at growing fodder yeast under conditions of aeration on a nutrient medium containing distillate stillage and mineral salts, extracting biomass by stepwise separation and rinsing from substances, bards, returning the culture liquid to the yeast cultivation stage, characterized in that and improving their quality, as well as enhancing the process, washing the biomass shanna culture of the yeast of the genus Candida from substances bards carry out soy et al. when recycled with culture serum, after which it is introduced into liquids (example 5) .. nutrient medium in the ratio of biomass intake is carried out by an alcohol-alcoholic bard: serum equal to by 3-step separation 20: 1 - 1: 1 (by volume). Tffl. the course of phosphoric acid by 14.3%. with washing the yeast suspension with soy whey after stage II. As a result, the increase in the yield of dry fodder yeast compared with the control was 2.5%, the content of crude protein in yeast increased by 3.0%, the ash content decreased by 0.9%, the water consumption decreased by 10%, and the course of phosphoric acid by 14.3%. Claims of the method of obtaining biomass, phosphoric acid consumption by 14.3%. looking at growing fodder yeast under aeration conditions on a nutrient medium containing an alcohol-containing bard and mineral salts, extracting biomass by stepwise separation and rinsing from substances, bards, returning the culture liquid to the yeast growing stage, characterized in that yeast and improvement of their quality, as well as the reduction of the process, washing the biomass from the substances bards carry out soy