JPS63284110A - Extract of seed of cuscuta japonica choisy - Google Patents

Abstract

PURPOSE:To obtain an extracted solution of seed of Cuscuta japonica choisy with a solvent and/or an extract of seed of Cuscuta japonica choisy prepared by distilling away the solvent, having promoting action on sebum secretion, mixable with cosmetic or external preparation for skin. CONSTITUTION:Powder of seed of Cuscuta japonica choisy known as a crude drug is extracted with a water-soluble solvent such as methanol, ethanol or water or an organic solvent such as n-hexane, chloroform, ethyl ether or benzene alone or a mixture thereof to give an extracted solution with a solvent or an extract. The extraction temperature may be low temperature, room temperature or high temperature. The amount of the extracted solution or the extract added to cosmetic or external preparation for skin is preferably 0.01-10wt.% calculated as the extract based on the sum amount.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Field of Application) The present invention relates to a solvent extract of sagebrush and/or an extract obtained by distilling off the solvent, which is characterized by having a sebum secretion promoting effect.

(Conventional technology) The youthfulness and beauty of skin and hair is largely due to sebum secretion, and the decline in sebum secretion as we age is one of the causes of rough skin and dry skin. It is considered. Substances that have a sebum secretion promoting effect, that is, a sebaceous gland cell activating effect, can activate the sebaceous gland function of human skin whose sebum secretion function has decreased and restore youthful skin. It is considered to be effective as a base for cosmetics etc. for the elderly.

It is also expected to be a useful topical agent for diseases thought to be caused by sebaceous gland atrophy or functional decline.

However, substances known as sebum secretion enhancers,
r-oryzanol [Mie Kobayashi et al., West Japan Dermatology (197
2) No. 566] and pantethine fatty acid esters (Japanese Patent Publication No. 19726/1982), there are only pedometers available, and at present there is no product that has a satisfactory effect. Therefore, a substance that particularly promotes sebum secretion is desired.

(Problems to be Solved by the Invention) As a result of intensive exploration and research in order to invent an even better sebum secretion promoter, the present inventors have found that a solvent extract of sagebrush, which is known as a crude drug, and/or a solvent thereof. The present invention was completed based on the discovery that the extract obtained by distilling off the sebum has the effect of significantly promoting sebum secretion.

(Means for solving the problem) The iris used in the present invention is from the family Convolvulaceae.
OhO1li7)
nenai@Lam), v Medaoshi (0, aua tr
alia R-BT-), Amadaoshi (a, sp
ilinum vein), and has been used as a herbal medicine with tonic and tonic effects since ancient times.

The solvent extract of Katsushiko of the present invention is prepared by extracting Katsushiko powder using a water-soluble solvent such as methanol, ethanol, water, etc.
- Extraction with organic solvents such as hexane, chloroform, ethyl ether, benzene, etc., or a mixture thereof.

These can be concentrated and diluted as necessary. Further, the extract of the present invention can be obtained by distilling off the solvent from each of the above-mentioned various solvent extracts according to a conventional method. The extraction operation (extraction temperature) may be performed at cold temperature, room temperature, or high temperature.

The solvent extract of the present invention and/or the extract obtained by distilling off the solvent can be blended into cosmetics or pharmaceuticals such as external skin preparations according to a conventional method, and the amount of blending is , usually 0.001 to 3 as an extract based on the total weight of cosmetics or external skin preparations, etc.
A suitable amount is 0% by weight, preferably 0.01 to 10% by weight.

If the amount is less than 0.001% by weight, a sufficient effect cannot be obtained, and if the amount exceeds 30% by weight, the effect will not be enhanced, which is uneconomical.

Example The present invention will be specifically described below with reference to Examples.

Note that the content of the present invention is not limited to these. The parts shown in the examples mean parts by weight, and the arcs mean parts by weight.

Example 1 Soak 100 g of Koitoko powder in 400 d of ethanol,
After heating under reflux for an hour, 4 sheets of paper 4. pass After washing the 4. hyperactivity with 200 liters of ethanol, the 4. solution and the washing solution were combined and concentrated under reduced pressure to obtain an ethanolic extract of iris.

Example 2 Soak 100 g of vine powder in 400 ml of ethanol,
After heating under reflux for 2 hours, 4. pass Step 4: After washing the excess fluid with 200 ml of ethanol, the solution and the washing solution were combined and concentrated to dryness under reduced pressure to obtain an ethanolic extract of Moito.

Example 3 After Soxhlet extraction of 100 g of iris using 400 ytl of n-hexane for 24 hours,
The liquid was concentrated under reduced pressure to obtain an n-hexane extract of iris.

Example 4 After 24 hours of Soxhlet extraction of 100 g of Koishi with 400 ml of n-hexane, the extract was filtered through a sheet of paper, and the liquid was concentrated to dryness under reduced pressure to obtain the n-hexane extract of Koishitoko. Obtained.

Example 5 0.1% cream ■Stearin! ! 40 parts ■ Cetyl alcohol 30 ■ Stearyl alcohol 10 ■ Liquid paraffin
6.5 ■White Vaseline 1
0.0 ■ Sorbitan monostearate) 15 ■ Polyoxyethylene (25) monostearate 3.0 ■ IA child ethanol extract 01 ■ Glycerin
50 [Phase] Suijoka Potassium
0.2■Methyl paraoxybenzoate
0.2 ■ Purified water 65.
5 [Manufacturing method] Ingredients ■～■ and ingredients ■～@70 each
After heating and dissolving at ~75°C, components Φ~■ are emulsified by adding components ■~@, and cooled to 30°C to form a product.

Example 6 1% combination ointment ■ Purified lanolin 5.0 parts ■ Sarashimiro 5.00 Uitoko n-
Hexane extract t00 white petrolatum
89.0 [Manufacturing method] After heating and melting each component and mixing, the product is cooled.

Example 7 Lotion containing α01-arc ■ Bottle thread water extract 1 part αo ■ Glycerin 4.0 ■ T3 butylene glycol 500 ethyl alcohol
7.00 Polyoxyethylene (20) Lauryl ether α5 ■ Methyl paraoxybenzoate α1 ■ Citric alcohol
α010 Sodium Citrate
α1 ■Fragrance
CLO5@Green No.3 αo
ooot ■ Add purified water to make 100.

[Manufacturing method] Components ⑦ to Φ, components Φ and ⑦ are mixed and dissolved. Separately, components (1), 01, (2), 0, and 0 are mixed and dissolved. Next, mix the two and use Teflon cloth (300 mesh I).
L) to make a product @Example 8 Two-stage combination emulsion ■1 Thread ethyl ether extract 2.0 @ ■ Squalane
5.00olive oil
5.0Φjojoba oil
5.00 cetyl alcohol
t5 ■ Glycerin monostearate 2.0 Polyoxyethylene (20) Ryuthyl ether 5.00 Lioxyethylene (20) Sorbitan monostearate 2.0 ■ Dipropylene glycol 55 [Co., Ltd.] Glycerin
5.50 fragrance
α1O paraoxybenzoyl methyl α3■
Add purified water to make 100 O. [Production method] Components (1) to (2) are heated and dissolved, mixed, and kept at 70°C to form an oil phase. Ingredients (1), (2), [Co., Ltd.], O and 0 are heated and dissolved, mixed and kept at 75°C to form an aqueous phase. Add the aqueous phase to the oil phase, then add ingredient (1) and homogeneously emulsify with a homomixer, then cool to 30°C while stirring to obtain a product.

(Effects of the Invention) The solvent extract of the threadworm of the present invention and/or the extract obtained by distilling off the solvent showed a remarkable sebum secretion promoting effect.

The effects of the present invention will be shown below through test examples.

Test Example 1 5 g of the ethanolic extract of the iris obtained in Example 2 was dissolved in 95 g of ethanol and used as a sample for the sebaceous gland activation effect test of the sebum secretion promoter of the present invention. A water extract and a methanol extract were also prepared in the same manner as in Example 2, and used as test samples as described above. In addition, 5 g of the n-hexane extract obtained in Example 4 was dissolved in 95 g of ethanol and used as a test sample of the present invention. An ethyl ether extract and a chloroform extract were also prepared in the same manner as in Example 4 and used as test samples as described above.

Experimental animals were engineered OR mice (male, 6 weeks old, average weight 2
Using hair clippers (6g), the entire area from the back to both sides of the abdomen was trimmed with clippers. Starting from the next day, the test sample of the present invention was applied to the skin of the test subject twice daily, once in the morning and once in the evening, α11j was applied each time. There were 8 animals in each group, and the sample was applied to the right flank of the experimental animals, and ethanol was applied to the left flank as a control. On the 11th day after the start of application, the mice were sacrificed, and samples and the skin of the ethanol-applied area were collected and fixed with 10q&neutral formalin. Histopathological specimens were prepared according to conventional methods, and double staining was performed with hematoxylene and eosin. Cells constituting sebaceous glands were counted as an indicator of sebaceous gland activation. To count sebaceous HIa cells, 15 arbitrary sebaceous glands were selected from Group Ia cells, and the number of cells constituting each sebaceous gland was counted under an optical microscope. The average value of the 15 samples was calculated, and the value was expressed as the number of sebaceous gland cells of the sample. In addition, the number of sebaceous gland cells in the ethanol-applied area of the same individual was measured in the same way, and the sample coating results for the cell number in the ethanol-applied area are shown in Table 1. All extracts significantly increased the number of sebaceous gland cells. . Furthermore, when compared with olive oil and γ-oryzanol, which is known as a cosmetic raw material with sebaceous gland activating effects, all extracts increased the number of sebaceous gland cells with a statistically significant difference.

As is clear from the results shown in Table 1, the extracts of the iris of the present invention using various solvents had a significant sebaceous gland cell activating effect, and were shown to have a sebum secretion promoting effect.

In addition, in the present invention, the extract of iris may be used alone, but for example, when used in combination with the extracts of sakatsu, toshi, senega, onna-sadako, and mulberry parasitic, which are known as herbal medicines,
A better sebaceous gland cell activating effect was obtained than when using the iris extract alone. Extracts of these herbal medicines can be used in combination with one or more of the extracts of sagebrush.

Test Example 2 0.1 of the vermilion ethanol extract obtained in Example 2 was applied externally to examine its sebum secretion promoting effect.

20 healthy people (10 men, 10 women, average age 34)
The test was conducted on people of all ages. First, 10 people applied an appropriate amount of a cream containing 0.1% to the inside of their left forearm, and an appropriate amount of cream containing no extract to the inside of their right forearm, three times a day for 30 consecutive days. Similarly, the other 10 subjects applied an appropriate amount of a 1% ointment on the inside of their right forearm and an ointment with the same formulation without extract on the inside of their left forearm three times a day for 30 consecutive days. On the 31st day, sebum secretion was measured using the one-cup method.

The results are shown in FIG. The area where the cream containing 0.1% Mao Shuzi ethanol extract was applied was compared to the area where the cream was not applied, and the ointment containing 1% Soon Shuzi n-hexane extract was compared to the non-containing ointment. In both cases, sebum secretion increased significantly.

[Brief explanation of the drawing]

FIG. 1 is a diagram showing the sebum secretion promoting effect when a cream containing 0.1% of Mao Zhuzi ethanol extract and an ointment containing 1% Moxibustion Zhuzi n-hexane extract were applied to humans.

Claims (1)

[Claims] A solvent extract of sagebrush and/or an extract obtained by distilling off the solvent, which is characterized by having a sebum secretion promoting effect.