JPS6234394B2 - - Google Patents
Info
- Publication number
- JPS6234394B2 JPS6234394B2 JP60069528A JP6952885A JPS6234394B2 JP S6234394 B2 JPS6234394 B2 JP S6234394B2 JP 60069528 A JP60069528 A JP 60069528A JP 6952885 A JP6952885 A JP 6952885A JP S6234394 B2 JPS6234394 B2 JP S6234394B2
- Authority
- JP
- Japan
- Prior art keywords
- gas
- perfusate
- culture
- column
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000012510 hollow fiber Substances 0.000 claims description 21
- 230000010412 perfusion Effects 0.000 claims description 15
- 238000010979 pH adjustment Methods 0.000 claims description 11
- 238000012136 culture method Methods 0.000 claims description 7
- 230000035699 permeability Effects 0.000 claims description 3
- 239000007789 gas Substances 0.000 description 55
- 210000004027 cell Anatomy 0.000 description 30
- 239000002609 medium Substances 0.000 description 11
- 238000000034 method Methods 0.000 description 9
- 239000001963 growth medium Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 239000012530 fluid Substances 0.000 description 5
- 238000013019 agitation Methods 0.000 description 4
- 238000004113 cell culture Methods 0.000 description 4
- 230000001276 controlling effect Effects 0.000 description 4
- 210000000056 organ Anatomy 0.000 description 4
- 238000005273 aeration Methods 0.000 description 3
- 210000004102 animal cell Anatomy 0.000 description 3
- 230000005587 bubbling Effects 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 238000003973 irrigation Methods 0.000 description 3
- 230000002262 irrigation Effects 0.000 description 3
- 238000009827 uniform distribution Methods 0.000 description 3
- 230000019522 cellular metabolic process Effects 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000005187 foaming Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 210000003437 trachea Anatomy 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60069528A JPS61227779A (ja) | 1985-04-02 | 1985-04-02 | 潅流培養方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60069528A JPS61227779A (ja) | 1985-04-02 | 1985-04-02 | 潅流培養方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61227779A JPS61227779A (ja) | 1986-10-09 |
| JPS6234394B2 true JPS6234394B2 (enrdf_load_stackoverflow) | 1987-07-27 |
Family
ID=13405311
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60069528A Granted JPS61227779A (ja) | 1985-04-02 | 1985-04-02 | 潅流培養方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61227779A (enrdf_load_stackoverflow) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01225475A (ja) * | 1988-03-07 | 1989-09-08 | Japan Gore Tex Inc | 培養装置 |
| JPH0269179A (ja) * | 1988-09-02 | 1990-03-08 | Nissho Corp | 細胞培養培地のガス交換方法 |
| JP2020171235A (ja) * | 2019-04-11 | 2020-10-22 | テルモ株式会社 | 細胞培養装置及びバイオリアクタ |
-
1985
- 1985-04-02 JP JP60069528A patent/JPS61227779A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS61227779A (ja) | 1986-10-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5126238A (en) | Hollow fiber cell propagation system and method | |
| US4973558A (en) | Method of culturing cells using highly gas saturated media | |
| US5656421A (en) | Multi-bioreactor hollow fiber cell propagation system and method | |
| CA1270455A (en) | Apparatus and method for culturing cells, removing waste and concentrating product | |
| US4650766A (en) | Culturing apparatus | |
| JP3420615B2 (ja) | 微生物を培養しおよびその代謝を利用し及び／又は維持するためのモジュール、モジュールの作動方法、肝臓維持システム、バイオリアクター、及び生物細胞生成物を製造する方法 | |
| US8906688B2 (en) | Cell expansion system and methods of use | |
| JPS63151306A (ja) | 生物的及び／又は薬物的な媒体を包含する物質移動のための装置及び方法 | |
| US5110741A (en) | Aeration apparatus for the culture of mammalian cells | |
| JPH0213365A (ja) | 中空繊維バイオリアクター培養システムおよび方法 | |
| JPS60210982A (ja) | 人間の、動物の、植物の細胞並びに雑種細胞および微生物を培養するための方法および装置 | |
| US7867761B2 (en) | Tray stack adapted for active gassing | |
| JPH04148675A (ja) | 細胞培養装置 | |
| JPS6234394B2 (enrdf_load_stackoverflow) | ||
| AU778141B2 (en) | Method for cultivating cells, a membrane module, utilization of a membrane module and reaction system for cultivation of said cells | |
| JP3412364B2 (ja) | 細胞培養装置及び細胞培養方法 | |
| JPS62130683A (ja) | 哺乳動物細胞を培養する方法および装置 | |
| CN110734858A (zh) | 一种多模式三维灌流式细胞培养仪 | |
| US6410307B1 (en) | Membrane module for testing active substances at cells | |
| US8557571B2 (en) | Reactor and reactor unit with hollow fibers | |
| JP3095464B2 (ja) | 細胞培養装置 | |
| EP1599571B1 (en) | A tray stack adapted for active gassing | |
| JP4200210B2 (ja) | 高効率バイオリアクターシステム | |
| JP2020171235A (ja) | 細胞培養装置及びバイオリアクタ | |
| JPS63123375A (ja) | 微細藻類培養装置 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| EXPY | Cancellation because of completion of term |