JPS61227779A - 潅流培養方法 - Google Patents
潅流培養方法Info
- Publication number
- JPS61227779A JPS61227779A JP60069528A JP6952885A JPS61227779A JP S61227779 A JPS61227779 A JP S61227779A JP 60069528 A JP60069528 A JP 60069528A JP 6952885 A JP6952885 A JP 6952885A JP S61227779 A JPS61227779 A JP S61227779A
- Authority
- JP
- Japan
- Prior art keywords
- gas
- perfusate
- culture
- column
- exchange column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000010412 perfusion Effects 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title description 8
- 239000012510 hollow fiber Substances 0.000 claims abstract description 22
- 238000012136 culture method Methods 0.000 claims description 7
- 238000010979 pH adjustment Methods 0.000 claims description 7
- 230000035699 permeability Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 abstract description 7
- 238000005273 aeration Methods 0.000 abstract description 5
- 230000005587 bubbling Effects 0.000 abstract description 4
- 238000013019 agitation Methods 0.000 abstract description 3
- 238000005187 foaming Methods 0.000 abstract description 3
- 239000007789 gas Substances 0.000 description 49
- 210000004027 cell Anatomy 0.000 description 29
- 239000001963 growth medium Substances 0.000 description 10
- 239000002609 medium Substances 0.000 description 8
- 238000004113 cell culture Methods 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- 230000001276 controlling effect Effects 0.000 description 4
- 210000004102 animal cell Anatomy 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 210000003437 trachea Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60069528A JPS61227779A (ja) | 1985-04-02 | 1985-04-02 | 潅流培養方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60069528A JPS61227779A (ja) | 1985-04-02 | 1985-04-02 | 潅流培養方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61227779A true JPS61227779A (ja) | 1986-10-09 |
| JPS6234394B2 JPS6234394B2 (enrdf_load_stackoverflow) | 1987-07-27 |
Family
ID=13405311
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60069528A Granted JPS61227779A (ja) | 1985-04-02 | 1985-04-02 | 潅流培養方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61227779A (enrdf_load_stackoverflow) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01225475A (ja) * | 1988-03-07 | 1989-09-08 | Japan Gore Tex Inc | 培養装置 |
| JPH0269179A (ja) * | 1988-09-02 | 1990-03-08 | Nissho Corp | 細胞培養培地のガス交換方法 |
| JP2020171235A (ja) * | 2019-04-11 | 2020-10-22 | テルモ株式会社 | 細胞培養装置及びバイオリアクタ |
-
1985
- 1985-04-02 JP JP60069528A patent/JPS61227779A/ja active Granted
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01225475A (ja) * | 1988-03-07 | 1989-09-08 | Japan Gore Tex Inc | 培養装置 |
| JPH0269179A (ja) * | 1988-09-02 | 1990-03-08 | Nissho Corp | 細胞培養培地のガス交換方法 |
| JP2020171235A (ja) * | 2019-04-11 | 2020-10-22 | テルモ株式会社 | 細胞培養装置及びバイオリアクタ |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6234394B2 (enrdf_load_stackoverflow) | 1987-07-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| EXPY | Cancellation because of completion of term |