JPS6234058A - フロ−サイトメ−タによる網状赤血球の測定方法 - Google Patents
フロ−サイトメ−タによる網状赤血球の測定方法Info
- Publication number
- JPS6234058A JPS6234058A JP60173009A JP17300985A JPS6234058A JP S6234058 A JPS6234058 A JP S6234058A JP 60173009 A JP60173009 A JP 60173009A JP 17300985 A JP17300985 A JP 17300985A JP S6234058 A JPS6234058 A JP S6234058A
- Authority
- JP
- Japan
- Prior art keywords
- reticulocytes
- fluorescence
- blood cells
- measuring
- rna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000001995 reticulocyte Anatomy 0.000 title claims abstract description 53
- 238000000034 method Methods 0.000 title claims abstract description 17
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 20
- KSCQDDRPFHTIRL-UHFFFAOYSA-N auramine O Chemical compound [H+].[Cl-].C1=CC(N(C)C)=CC=C1C(=N)C1=CC=C(N(C)C)C=C1 KSCQDDRPFHTIRL-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000007850 fluorescent dye Substances 0.000 claims abstract description 7
- 210000000601 blood cell Anatomy 0.000 claims description 20
- UIZLQMLDSWKZGC-UHFFFAOYSA-N cadmium helium Chemical compound [He].[Cd] UIZLQMLDSWKZGC-UHFFFAOYSA-N 0.000 claims 1
- 230000001678 irradiating effect Effects 0.000 claims 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 claims 1
- 229910052753 mercury Inorganic materials 0.000 claims 1
- 229910052724 xenon Inorganic materials 0.000 claims 1
- FHNFHKCVQCLJFQ-UHFFFAOYSA-N xenon atom Chemical compound [Xe] FHNFHKCVQCLJFQ-UHFFFAOYSA-N 0.000 claims 1
- 239000000975 dye Substances 0.000 abstract description 21
- 210000004369 blood Anatomy 0.000 abstract description 16
- 239000008280 blood Substances 0.000 abstract description 16
- JPIYZTWMUGTEHX-UHFFFAOYSA-N auramine O free base Chemical compound C1=CC(N(C)C)=CC=C1C(=N)C1=CC=C(N(C)C)C=C1 JPIYZTWMUGTEHX-UHFFFAOYSA-N 0.000 abstract description 12
- 210000004027 cell Anatomy 0.000 abstract description 3
- 239000003146 anticoagulant agent Substances 0.000 abstract description 2
- 229940127219 anticoagulant drug Drugs 0.000 abstract description 2
- 230000001376 precipitating effect Effects 0.000 abstract 2
- 210000003743 erythrocyte Anatomy 0.000 description 29
- 238000010186 staining Methods 0.000 description 20
- 238000005259 measurement Methods 0.000 description 13
- 239000000523 sample Substances 0.000 description 13
- 239000000243 solution Substances 0.000 description 9
- DPKHZNPWBDQZCN-UHFFFAOYSA-N acridine orange free base Chemical compound C1=CC(N(C)C)=CC2=NC3=CC(N(C)C)=CC=C3C=C21 DPKHZNPWBDQZCN-UHFFFAOYSA-N 0.000 description 8
- DZBUGLKDJFMEHC-UHFFFAOYSA-N benzoquinolinylidene Natural products C1=CC=CC2=CC3=CC=CC=C3N=C21 DZBUGLKDJFMEHC-UHFFFAOYSA-N 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000010790 dilution Methods 0.000 description 4
- 239000012895 dilution Substances 0.000 description 4
- INCIMLINXXICKS-UHFFFAOYSA-M pyronin Y Chemical compound [Cl-].C1=CC(=[N+](C)C)C=C2OC3=CC(N(C)C)=CC=C3C=C21 INCIMLINXXICKS-UHFFFAOYSA-M 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- -1 alkali metal salt Chemical class 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 210000003660 reticulum Anatomy 0.000 description 3
- 239000012488 sample solution Substances 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 238000001179 sorption measurement Methods 0.000 description 3
- 239000012192 staining solution Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- JADVWWSKYZXRGX-UHFFFAOYSA-M thioflavine T Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C1=[N+](C)C2=CC=C(C)C=C2S1 JADVWWSKYZXRGX-UHFFFAOYSA-M 0.000 description 3
- 230000000007 visual effect Effects 0.000 description 3
- FTOAOBMCPZCFFF-UHFFFAOYSA-N 5,5-diethylbarbituric acid Chemical compound CCC1(CC)C(=O)NC(=O)NC1=O FTOAOBMCPZCFFF-UHFFFAOYSA-N 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 229910052783 alkali metal Inorganic materials 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 230000005284 excitation Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 238000005375 photometry Methods 0.000 description 2
- 238000006862 quantum yield reaction Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- QTWJRLJHJPIABL-UHFFFAOYSA-N 2-methylphenol;3-methylphenol;4-methylphenol Chemical compound CC1=CC=C(O)C=C1.CC1=CC=CC(O)=C1.CC1=CC=CC=C1O QTWJRLJHJPIABL-UHFFFAOYSA-N 0.000 description 1
- 208000032467 Aplastic anaemia Diseases 0.000 description 1
- 208000032843 Hemorrhage Diseases 0.000 description 1
- 206010021074 Hypoplastic anaemia Diseases 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 230000004570 RNA-binding Effects 0.000 description 1
- 206010041235 Snoring Diseases 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- XKRFYHLGVUSROY-UHFFFAOYSA-N argon Substances [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 1
- 229910052786 argon Inorganic materials 0.000 description 1
- 229960002319 barbital Drugs 0.000 description 1
- 239000000981 basic dye Substances 0.000 description 1
- 208000034158 bleeding Diseases 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 238000004040 coloring Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 229930003836 cresol Natural products 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- SQHOAFZGYFNDQX-UHFFFAOYSA-N ethyl-[7-(ethylamino)-2,8-dimethylphenothiazin-3-ylidene]azanium;chloride Chemical compound [Cl-].S1C2=CC(=[NH+]CC)C(C)=CC2=NC2=C1C=C(NCC)C(C)=C2 SQHOAFZGYFNDQX-UHFFFAOYSA-N 0.000 description 1
- 239000000834 fixative Substances 0.000 description 1
- 208000007475 hemolytic anemia Diseases 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
- G01N2015/012—Red blood cells
- G01N2015/014—Reticulocytes
Landscapes
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60173009A JPS6234058A (ja) | 1985-08-06 | 1985-08-06 | フロ−サイトメ−タによる網状赤血球の測定方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP60173009A JPS6234058A (ja) | 1985-08-06 | 1985-08-06 | フロ−サイトメ−タによる網状赤血球の測定方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6234058A true JPS6234058A (ja) | 1987-02-14 |
| JPH0464588B2 JPH0464588B2 (enrdf_load_stackoverflow) | 1992-10-15 |
Family
ID=15952502
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP60173009A Granted JPS6234058A (ja) | 1985-08-06 | 1985-08-06 | フロ−サイトメ−タによる網状赤血球の測定方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6234058A (enrdf_load_stackoverflow) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4981803A (en) * | 1987-07-31 | 1991-01-01 | Toa Medical Electronics Co., Ltd. | Reagent for reticulocyte counting by flow cytometry |
| JPH05184742A (ja) * | 1992-01-16 | 1993-07-27 | Kaijirushi Hamono Kaihatsu Center:Kk | 安全かみそり |
| US5260764A (en) * | 1990-02-08 | 1993-11-09 | Toa Medical Electronics Co., Ltd. | Optical particle analyzing apparatus having two types of light source |
| EP0613003A1 (en) * | 1993-02-22 | 1994-08-31 | Toa Medical Electronics Co., Ltd. | A reagent for detecting malaria infected cells and a detecting method for malaria infected cells using the same |
| JP2014206551A (ja) * | 2014-08-07 | 2014-10-30 | ソニー株式会社 | データ表示方法、プログラム、データ解析装置及び微小粒子分析システム |
| US9619907B2 (en) | 2010-04-28 | 2017-04-11 | Sony Corporation | Microparticle analyzing apparatus and data displaying method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4336029A (en) * | 1980-08-15 | 1982-06-22 | Ortho Diagnostic Systems Inc. | Method and reagents for quantitative determination of reticulocytes and platelets in whole blood |
-
1985
- 1985-08-06 JP JP60173009A patent/JPS6234058A/ja active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4336029A (en) * | 1980-08-15 | 1982-06-22 | Ortho Diagnostic Systems Inc. | Method and reagents for quantitative determination of reticulocytes and platelets in whole blood |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4981803A (en) * | 1987-07-31 | 1991-01-01 | Toa Medical Electronics Co., Ltd. | Reagent for reticulocyte counting by flow cytometry |
| US5260764A (en) * | 1990-02-08 | 1993-11-09 | Toa Medical Electronics Co., Ltd. | Optical particle analyzing apparatus having two types of light source |
| JPH05184742A (ja) * | 1992-01-16 | 1993-07-27 | Kaijirushi Hamono Kaihatsu Center:Kk | 安全かみそり |
| EP0613003A1 (en) * | 1993-02-22 | 1994-08-31 | Toa Medical Electronics Co., Ltd. | A reagent for detecting malaria infected cells and a detecting method for malaria infected cells using the same |
| US5470751A (en) * | 1993-02-22 | 1995-11-28 | Toa Medical Electronics Co., Ltd. | Reagent for detecting malaria infected cells and a detecting method for malaria infected cells using the same |
| US9619907B2 (en) | 2010-04-28 | 2017-04-11 | Sony Corporation | Microparticle analyzing apparatus and data displaying method |
| US10147209B2 (en) | 2010-04-28 | 2018-12-04 | Sony Corporation | Microparticle analyzing apparatus and data displaying method |
| US11074727B2 (en) | 2010-04-28 | 2021-07-27 | Sony Corporation | Microparticle analyzing apparatus and data displaying method |
| US11727612B2 (en) | 2010-04-28 | 2023-08-15 | Sony Corporation | Microparticle analyzing apparatus and data displaying method |
| JP2014206551A (ja) * | 2014-08-07 | 2014-10-30 | ソニー株式会社 | データ表示方法、プログラム、データ解析装置及び微小粒子分析システム |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0464588B2 (enrdf_load_stackoverflow) | 1992-10-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |