JPS6212990B2 - - Google Patents
Info
- Publication number
- JPS6212990B2 JPS6212990B2 JP2156384A JP2156384A JPS6212990B2 JP S6212990 B2 JPS6212990 B2 JP S6212990B2 JP 2156384 A JP2156384 A JP 2156384A JP 2156384 A JP2156384 A JP 2156384A JP S6212990 B2 JPS6212990 B2 JP S6212990B2
- Authority
- JP
- Japan
- Prior art keywords
- culture
- cell culture
- cells
- suspension
- units
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 238000004113 cell culture Methods 0.000 claims description 28
- 239000012510 hollow fiber Substances 0.000 claims description 15
- 239000012528 membrane Substances 0.000 claims description 14
- 235000015097 nutrients Nutrition 0.000 claims description 9
- 239000012466 permeate Substances 0.000 claims description 5
- 238000007599 discharging Methods 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 230000002401 inhibitory effect Effects 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 description 28
- 239000000243 solution Substances 0.000 description 27
- 239000000725 suspension Substances 0.000 description 20
- 239000001963 growth medium Substances 0.000 description 11
- 239000007788 liquid Substances 0.000 description 9
- 238000012258 culturing Methods 0.000 description 6
- 239000011148 porous material Substances 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 239000006143 cell culture medium Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 238000004114 suspension culture Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000003094 microcapsule Substances 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- UBAZGMLMVVQSCD-UHFFFAOYSA-N carbon dioxide;molecular oxygen Chemical compound O=O.O=C=O UBAZGMLMVVQSCD-UHFFFAOYSA-N 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 238000012007 large scale cell culture Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000002503 metabolic effect Effects 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 206010035226 Plasma cell myeloma Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 210000000628 antibody-producing cell Anatomy 0.000 description 1
- 239000003443 antiviral agent Substances 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000012930 cell culture fluid Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000012531 culture fluid Substances 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229910052731 fluorine Inorganic materials 0.000 description 1
- 239000011737 fluorine Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 201000000050 myeloid neoplasm Diseases 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920002492 poly(sulfone) Polymers 0.000 description 1
- 229920002239 polyacrylonitrile Polymers 0.000 description 1
- 230000002062 proliferating effect Effects 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 239000012679 serum free medium Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2156384A JPS60168379A (ja) | 1984-02-10 | 1984-02-10 | 細胞培養装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2156384A JPS60168379A (ja) | 1984-02-10 | 1984-02-10 | 細胞培養装置 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60168379A JPS60168379A (ja) | 1985-08-31 |
| JPS6212990B2 true JPS6212990B2 (ko) | 1987-03-23 |
Family
ID=12058479
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2156384A Granted JPS60168379A (ja) | 1984-02-10 | 1984-02-10 | 細胞培養装置 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60168379A (ko) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06102013B2 (ja) * | 1987-03-27 | 1994-12-14 | 宇部興産株式会社 | バイオリアクタ− |
| US5002890A (en) * | 1988-11-29 | 1991-03-26 | The United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration | Spiral vane bioreactor |
-
1984
- 1984-02-10 JP JP2156384A patent/JPS60168379A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS60168379A (ja) | 1985-08-31 |
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