JPS6136915B2 - - Google Patents

Info

Publication number

JPS6136915B2

JPS6136915B2 JP57189243A JP18924382A JPS6136915B2 JP S6136915 B2 JPS6136915 B2 JP S6136915B2 JP 57189243 A JP57189243 A JP 57189243A JP 18924382 A JP18924382 A JP 18924382A JP S6136915 B2 JPS6136915 B2 JP S6136915B2

Authority

JP

Japan

Prior art keywords

cells

culture

medium

cell

tube

Prior art date

1982-10-29

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Expired

Links

• Espacenet
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• 238000004062 sedimentation Methods 0.000 claims description 20
• 239000012737 fresh medium Substances 0.000 claims description 13
• 239000012228 culture supernatant Substances 0.000 claims description 10
• 238000000034 method Methods 0.000 claims description 10
• 238000012258 culturing Methods 0.000 claims description 7
• 239000001963 growth medium Substances 0.000 claims description 6
• 238000004114 suspension culture Methods 0.000 claims description 6
• 238000012136 culture method Methods 0.000 claims description 3
• 210000004027 cell Anatomy 0.000 description 82
• 239000002609 medium Substances 0.000 description 19
• 238000003756 stirring Methods 0.000 description 8
• 239000006228 supernatant Substances 0.000 description 8
• 238000001556 precipitation Methods 0.000 description 6
• 239000011521 glass Substances 0.000 description 4
• 229910001220 stainless steel Inorganic materials 0.000 description 4
• 239000010935 stainless steel Substances 0.000 description 4
• 239000004809 Teflon Substances 0.000 description 3
• 229920006362 Teflon® Polymers 0.000 description 3
• 239000000047 product Substances 0.000 description 3
• 238000005070 sampling Methods 0.000 description 3
• 210000002966 serum Anatomy 0.000 description 3
• 239000002699 waste material Substances 0.000 description 3
• 241001465754 Metazoa Species 0.000 description 2
• UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
• 238000004113 cell culture Methods 0.000 description 2
• 230000005484 gravity Effects 0.000 description 2
• 238000011081 inoculation Methods 0.000 description 2
• NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 2
• 230000002572 peristaltic effect Effects 0.000 description 2
• 239000012679 serum free medium Substances 0.000 description 2
• DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 2
• 230000004083 survival effect Effects 0.000 description 2
• 102000004877 Insulin Human genes 0.000 description 1
• 108090001061 Insulin Proteins 0.000 description 1
• 239000012980 RPMI-1640 medium Substances 0.000 description 1
• 102000004338 Transferrin Human genes 0.000 description 1
• 108090000901 Transferrin Proteins 0.000 description 1
• 238000005273 aeration Methods 0.000 description 1
• 210000004102 animal cell Anatomy 0.000 description 1
• 239000007640 basal medium Substances 0.000 description 1
• 244000309466 calf Species 0.000 description 1
• 239000006143 cell culture medium Substances 0.000 description 1
• 230000010261 cell growth Effects 0.000 description 1
• 238000005119 centrifugation Methods 0.000 description 1
• 238000006243 chemical reaction Methods 0.000 description 1
• 239000012141 concentrate Substances 0.000 description 1
• 210000004748 cultured cell Anatomy 0.000 description 1
• 230000003247 decreasing effect Effects 0.000 description 1
• BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
• 210000002919 epithelial cell Anatomy 0.000 description 1
• 230000012010 growth Effects 0.000 description 1
• 229940125396 insulin Drugs 0.000 description 1
• 210000000265 leukocyte Anatomy 0.000 description 1
• 239000007788 liquid Substances 0.000 description 1
• 210000004698 lymphocyte Anatomy 0.000 description 1
• 244000005700 microbiome Species 0.000 description 1
• 210000000066 myeloid cell Anatomy 0.000 description 1
• 239000004033 plastic Substances 0.000 description 1
• 238000000926 separation method Methods 0.000 description 1
• 235000017557 sodium bicarbonate Nutrition 0.000 description 1
• 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
• 229940054269 sodium pyruvate Drugs 0.000 description 1
• 229960001471 sodium selenite Drugs 0.000 description 1
• 235000015921 sodium selenite Nutrition 0.000 description 1
• 239000011781 sodium selenite Substances 0.000 description 1
• 239000000725 suspension Substances 0.000 description 1
• 239000012581 transferrin Substances 0.000 description 1
• 238000009423 ventilation Methods 0.000 description 1
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1