JPS61212287A - 担体―固定化蛋白質複合体及びその製造方法 - Google Patents
担体―固定化蛋白質複合体及びその製造方法Info
- Publication number
- JPS61212287A JPS61212287A JP5277686A JP5277686A JPS61212287A JP S61212287 A JPS61212287 A JP S61212287A JP 5277686 A JP5277686 A JP 5277686A JP 5277686 A JP5277686 A JP 5277686A JP S61212287 A JPS61212287 A JP S61212287A
- Authority
- JP
- Japan
- Prior art keywords
- carrier
- solution
- protein
- immobilized protein
- group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102000004169 proteins and genes Human genes 0.000 title claims description 34
- 108090000623 proteins and genes Proteins 0.000 title claims description 34
- 239000012528 membrane Substances 0.000 claims description 28
- 102000004190 Enzymes Human genes 0.000 claims description 23
- 108090000790 Enzymes Proteins 0.000 claims description 23
- 238000012360 testing method Methods 0.000 claims description 21
- 229940088598 enzyme Drugs 0.000 claims description 20
- 108010015776 Glucose oxidase Proteins 0.000 claims description 12
- 239000004366 Glucose oxidase Substances 0.000 claims description 12
- 229940116332 glucose oxidase Drugs 0.000 claims description 12
- 235000019420 glucose oxidase Nutrition 0.000 claims description 12
- 239000004952 Polyamide Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 229920002647 polyamide Polymers 0.000 claims description 10
- 102000003992 Peroxidases Human genes 0.000 claims description 9
- 108040007629 peroxidase activity proteins Proteins 0.000 claims description 9
- -1 phosphoryl group Chemical group 0.000 claims description 9
- 125000000524 functional group Chemical group 0.000 claims description 8
- 229920002678 cellulose Polymers 0.000 claims description 7
- 239000001913 cellulose Substances 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 239000012460 protein solution Substances 0.000 claims description 4
- 239000011942 biocatalyst Substances 0.000 claims description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 3
- 125000004076 pyridyl group Chemical group 0.000 claims description 2
- 108010050375 Glucose 1-Dehydrogenase Proteins 0.000 claims 1
- 108010058683 Immobilized Proteins Proteins 0.000 claims 1
- 125000003277 amino group Chemical group 0.000 claims 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims 1
- 125000001841 imino group Chemical group [H]N=* 0.000 claims 1
- 125000000542 sulfonic acid group Chemical group 0.000 claims 1
- 125000004149 thio group Chemical group *S* 0.000 claims 1
- 239000000243 solution Substances 0.000 description 42
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 19
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 17
- 239000008103 glucose Substances 0.000 description 17
- 239000010410 layer Substances 0.000 description 15
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 8
- 108010010803 Gelatin Proteins 0.000 description 8
- 239000011248 coating agent Substances 0.000 description 8
- 238000000576 coating method Methods 0.000 description 8
- 239000008273 gelatin Substances 0.000 description 8
- 229920000159 gelatin Polymers 0.000 description 8
- 235000019322 gelatine Nutrition 0.000 description 8
- 235000011852 gelatine desserts Nutrition 0.000 description 8
- 239000012085 test solution Substances 0.000 description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
- 239000000969 carrier Substances 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- HWYHZTIRURJOHG-UHFFFAOYSA-N luminol Chemical compound O=C1NNC(=O)C2=C1C(N)=CC=C2 HWYHZTIRURJOHG-UHFFFAOYSA-N 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 229920000642 polymer Polymers 0.000 description 5
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000007864 aqueous solution Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000000499 gel Substances 0.000 description 3
- 238000004020 luminiscence type Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 3
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- BPYKTIZUTYGOLE-IFADSCNNSA-N Bilirubin Chemical compound N1C(=O)C(C)=C(C=C)\C1=C\C1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(\C=C/3C(=C(C=C)C(=O)N\3)C)N2)CCC(O)=O)N1 BPYKTIZUTYGOLE-IFADSCNNSA-N 0.000 description 2
- 229920002302 Nylon 6,6 Polymers 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000002745 absorbent Effects 0.000 description 2
- 239000002250 absorbent Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000000712 assembly Effects 0.000 description 2
- 238000000429 assembly Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000007385 chemical modification Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 239000006185 dispersion Substances 0.000 description 2
- 150000004677 hydrates Chemical class 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 125000000325 methylidene group Chemical group [H]C([H])=* 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- QXCFFLGAAJDPTG-UHFFFAOYSA-M sodium;2,3-dichlorobenzenesulfonate Chemical compound [Na+].[O-]S(=O)(=O)C1=CC=CC(Cl)=C1Cl QXCFFLGAAJDPTG-UHFFFAOYSA-M 0.000 description 2
- 125000001424 substituent group Chemical group 0.000 description 2
- OBHRVMZSZIDDEK-UHFFFAOYSA-N urobilinogen Chemical compound CCC1=C(C)C(=O)NC1CC1=C(C)C(CCC(O)=O)=C(CC2=C(C(C)=C(CC3C(=C(CC)C(=O)N3)C)N2)CCC(O)=O)N1 OBHRVMZSZIDDEK-UHFFFAOYSA-N 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- JJMQRJKPLUACSO-UHFFFAOYSA-N 3-(4-iodophenyl)-2-(4-nitrophenyl)-5-phenyl-1,3-dihydrotetrazol-3-ium;chloride Chemical compound [Cl-].C1=CC([N+](=O)[O-])=CC=C1N1N(C=2C=CC(I)=CC=2)[NH2+]C(C=2C=CC=CC=2)=N1 JJMQRJKPLUACSO-UHFFFAOYSA-N 0.000 description 1
- QIGJYVCQYDKYDW-UHFFFAOYSA-N 3-O-alpha-D-mannopyranosyl-D-mannopyranose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(CO)OC(O)C1O QIGJYVCQYDKYDW-UHFFFAOYSA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- RLFWWDJHLFCNIJ-UHFFFAOYSA-N Aminoantipyrine Natural products CN1C(C)=C(N)C(=O)N1C1=CC=CC=C1 RLFWWDJHLFCNIJ-UHFFFAOYSA-N 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- 108010093096 Immobilized Enzymes Proteins 0.000 description 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- 229920004011 Macrolon® Polymers 0.000 description 1
- 239000004425 Makrolon Substances 0.000 description 1
- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 description 1
- 229920002292 Nylon 6 Polymers 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 101710132772 Peroxidase 1 Proteins 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 235000013334 alcoholic beverage Nutrition 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 125000004429 atom Chemical group 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 125000006367 bivalent amino carbonyl group Chemical group [H]N([*:1])C([*:2])=O 0.000 description 1
- 239000012876 carrier material Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000001112 coagulating effect Effects 0.000 description 1
- VDBXLXRWMYNMHL-UHFFFAOYSA-N decanediamide Chemical compound NC(=O)CCCCCCCCC(N)=O VDBXLXRWMYNMHL-UHFFFAOYSA-N 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- YRIUSKIDOIARQF-UHFFFAOYSA-N dodecyl benzenesulfonate Chemical compound CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 YRIUSKIDOIARQF-UHFFFAOYSA-N 0.000 description 1
- 229940071161 dodecylbenzenesulfonate Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- JBKVHLHDHHXQEQ-UHFFFAOYSA-N epsilon-caprolactam Chemical compound O=C1CCCCCN1 JBKVHLHDHHXQEQ-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 150000002367 halogens Chemical class 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 238000005470 impregnation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000004745 nonwoven fabric Substances 0.000 description 1
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 239000004417 polycarbonate Substances 0.000 description 1
- 229920006267 polyester film Polymers 0.000 description 1
- 229920000573 polyethylene Polymers 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 125000000472 sulfonyl group Chemical group *S(*)(=O)=O 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- NEUOBESLMIKJSB-UHFFFAOYSA-J tetrasodium;tetraacetate Chemical compound [Na+].[Na+].[Na+].[Na+].CC([O-])=O.CC([O-])=O.CC([O-])=O.CC([O-])=O NEUOBESLMIKJSB-UHFFFAOYSA-J 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3508908 | 1985-03-13 | ||
| DE3508908.3 | 1985-03-13 | ||
| DE3529094.3 | 1985-08-14 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS61212287A true JPS61212287A (ja) | 1986-09-20 |
| JPH0311759B2 JPH0311759B2 (enExample) | 1991-02-18 |
Family
ID=6265034
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP5277686A Granted JPS61212287A (ja) | 1985-03-13 | 1986-03-12 | 担体―固定化蛋白質複合体及びその製造方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS61212287A (enExample) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009080106A (ja) * | 2000-10-10 | 2009-04-16 | Biotrove Inc | アッセイ、合成、および保存用の器具、ならびに、その作製、使用、および操作の方法 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103333350B (zh) * | 2013-06-09 | 2015-05-20 | 浙江大学 | 一种丝胶微球的制备方法 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54154595A (en) * | 1978-05-22 | 1979-12-05 | Unitika Ltd | Novel carrier for fixing physiologically active substance |
| JPS58212786A (ja) * | 1982-06-04 | 1983-12-10 | Daicel Chem Ind Ltd | 高活性固体化生体触媒の製造方法 |
| JPS59151050A (ja) * | 1983-02-17 | 1984-08-29 | Matsushita Electric Works Ltd | 生体触媒電極の製法 |
-
1986
- 1986-03-12 JP JP5277686A patent/JPS61212287A/ja active Granted
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54154595A (en) * | 1978-05-22 | 1979-12-05 | Unitika Ltd | Novel carrier for fixing physiologically active substance |
| JPS58212786A (ja) * | 1982-06-04 | 1983-12-10 | Daicel Chem Ind Ltd | 高活性固体化生体触媒の製造方法 |
| JPS59151050A (ja) * | 1983-02-17 | 1984-08-29 | Matsushita Electric Works Ltd | 生体触媒電極の製法 |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009080106A (ja) * | 2000-10-10 | 2009-04-16 | Biotrove Inc | アッセイ、合成、および保存用の器具、ならびに、その作製、使用、および操作の方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0311759B2 (enExample) | 1991-02-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4808529A (en) | Enzymes immobilized on polyamides or cellulose hydrate | |
| RU2288273C2 (ru) | Композиция реагентов, реагентная тест-полоска, система, способ и набор для обнаружения или определения присутствия аналита в физиологическом образце | |
| US4824639A (en) | Test device and a method for the detection of a component of a liquid sample | |
| JP3220486B2 (ja) | 血液グルコース濃度用可視試験片 | |
| CA1056746A (en) | Biologically active membrane material | |
| US4153668A (en) | Multi-zone analytical element and method using same | |
| US3975162A (en) | Applying reagent to medium and device therefor | |
| US5403706A (en) | Carrier matrix with dissolvably impregnated reagent | |
| CA2486950C (en) | Method for producing polymer layers | |
| JPH02262600A (ja) | 固相への蛋白質固定化法、分析測定法及び固相 | |
| JPS5921500B2 (ja) | 酸素電極用酵素膜 | |
| JP2685145B2 (ja) | 酵素電極 | |
| AU633210B2 (en) | Asymmetric sandwich membrane system as diagnostic test device | |
| JPH0622760A (ja) | 生化学物質の固定化方法 | |
| JPS61212287A (ja) | 担体―固定化蛋白質複合体及びその製造方法 | |
| AU592771B2 (en) | Device composed of polymers with a membrane structure and incorporated solids particles | |
| JP2708590B2 (ja) | 生物学的に活性な分子が固定化された酵素膜，ｅｌｉｓａ膜，センサ膜 | |
| EP3449013B1 (en) | Testing device and method for producing same, testing method, and testing kit and transfer medium for producing testing device | |
| US5242793A (en) | Selective permeable membrane and electrode using the same | |
| WO1988009824A1 (en) | Improvements in diagnostic test strips | |
| JP2648361B2 (ja) | 選択透過膜およびそれを用いる電極 | |
| WO2004088316A1 (en) | Method for preparing photoreactive polymers for immobilizing biomolecules thereon | |
| EP0370417B1 (en) | Biological diagnostic assay system | |
| JPH01101453A (ja) | 被分析物質の検出方法 | |
| JPH0518959A (ja) | 分析素子 |