JPS6061559A - Concentration of natural taurine - Google Patents

Abstract

PURPOSE:Macromolecular components are removed from stickwater which is obtained in the production of fish meal to give a concentrate of natural taurine used in health food in high concentration. CONSTITUTION:Stickwater obtained in the production of fish meal, e.g., obtained from walley pollack, fish, sardine or mackerel is treated to remove macromolecular components to collect a concentrate containing natural taurine. Taurine is a sulfur-containing aminoacid and widely distributes in living tissues and a variety of pharmaceutical actions have been observed, but its synthetic product cannot be used, therefore we have to rely on the natural product. The process according to the present invention gives a concentrate containing more than 10% of aminoacids on the solid basis from stickwater and the concentrated taurine is separated from other aminoacids by electrodialysis, resulting in the product of extremely high purity.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention relates to a method for producing natural taurine, and more particularly to a method for obtaining a concentrate containing a high concentration of natural taurine from stick water obtained during the production of fishmeal.

Taurine is a sulfur-containing amino acid with a structure of H, N -OH, OH, -So, H (molecular weight 125), and is widely distributed in the living tissues of animals.
It has been recognized that it has pharmacological effects such as anti-arteriosclerotic, cholesterol-lowering, central nervous system depression, vision preservation, and blood sugar rise suppressing effects.

Synthetic products of taurine are currently on the market and are permitted to be used in medicines, but because they are synthetic, their use in foods is not permitted at all.

For these reasons, there are many requests to use natural taurine as a health food, and attempts have been made to extract it from natural products, but currently only oyster extract exists, and the taurine concentration is extremely low. The production volume is also modest.

On the other hand, it is known that taurine naturally exists in large amounts in cow bile, but since it combines with cholic acid and exists as taurocholic acid, it is difficult to separate and collect cytaurine. It was difficult.

Under these circumstances, the inventor has investigated the presence of taurine in a wide range of natural products, and has found that a large amount of taurine exists in a free state in marine products, and that this is present in the stick water that is separated during the production of meal. I found out that there is a transition to Furthermore, the non-inventor discovered that, among the stick waters of various marine products, the stick water obtained during the production of fishmeal has a higher content of taurine in its free amino acids than other stick waters, as shown in Table 1. He discovered that this is extremely advantageous in the production of taurine, and completed his invention.

Table 1 Accordingly, the uninvention is to remove the polymeric components from the stick water d- obtained during the production of fishmeal e4? It provides a method for concentrating natural taurine, which is a distinctive feature of taurine. 1.Fitschmir is produced in large quantities with domestic production of 670,000 tons according to statistics in 1981, and the stick water produced as a by-product of this process is Some of it is concentrated to about 50% water and returned to feed as fish soluple or used as fertilizer, but the majority of it is actually discarded.

Currently, Fitzshmir is manufactured in the North Pacific Ocean using cod, flounder, etc. as raw materials. The main product is coastal meal (commonly known as Nibrown meal). The stick water obtained when producing white fish meal and brown meal contains a large amount of taurine, and there is no significant difference in the free amino acid composition and the taurine content in the free amino acids. I can't do it. Therefore, any stick water can be used as a non-inventive raw material, but the former white fishmeal stick water is particularly preferable since a large amount of raw material can be secured.

Normally, the muscle parts of captured fish are collected as raw material for frozen meat, and the remains of the head, internal organs, skin, bones, etc., and the whole body of small fish (unsuitable as raw material for soup stock) are fed to a steam cutter. It is then steamed. The steamed product is sent to a screw press along with the broth, where it is squeezed and separated into press liquid and press cake. The press cake is dried to produce fishmeal, and the press liquid is decanted to remove sludge, and then treated with an oil separator to separate and remove most of the oil to obtain stick water. This stick water is concentrated to less than 50% moisture in a multilayer evaporator and is called solupuru, but in the present invention, this soluble can also be used as a raw material, and in the unknown specification, the stick water including solupuru is referred to as solupuru. It is called water.

Although it varies depending on the type of fish, in addition to free amino acids, this stick water contains about 5% of polymer components.
Contains 0% (on solids) protein and peptides, approximately 20% (on solids) lipids and ash.

According to the uninvented method, these high molecular compounds are removed from stick water as much as possible to obtain a concentrate having a free amino acid content of 10% or more in solid matter. Methods known per se can be employed to remove the polymer compounds, such as precipitation using a flocculant or acid treatment, ultrafiltration, ion exchange chromatography, reverse osmosis, ultracentrifugation, and gel filtration. This can be achieved by performing the above alone or in combination.

Natural taurine condensed in this manner can be separated from other free amino acids by methods such as electrodialysis, ion exchange resin, and ethanol recrystallization, and can be obtained in extremely high purity.

Next, an example will be given and explained.

Example 1 In the process of producing Hokuyo Fitzschmeal using the meat residue of a 9-fish cod carpenter as raw material, the sap portion ( Sludge (commonly known as press liquid) was removed using a sludge separator, and oil was further removed using an oil separator to prepare stick water.

Its composition is shown in Table 2.

Table 2 Composition of North Sea Stick Water (@(1)
U indicates solid content (2) (total amino acid content) - (free amino acid content) 2 ol of this stick water! was subjected to ultrafiltration under the following conditions to obtain a pale yellow clear p filtrate 161.

Equipment Nikitto Denko Co., Ltd. Ultrafiltration tester UltraFJ membrane: NTU-2006 (molecular weight cut off 6,00
0), tubular membrane pressure 2.5 K9/c1F? Flow rate: asx/min Temperature: 40° C. The composition of the *V=filtrate thus obtained is as shown in Table 3.

Table 3 Composition of filtrate (% of solids) Reference example 1゜Example 1. The filtrate 16A' obtained in step 16A' was concentrated under reduced pressure until the concentration of
．． 2 ctn x height 36 cm) was subjected to ion exchange treatment, the eluate was heated under reduced pressure to remove all water, and 5 times the amount of 987/V% ethanol was added to the residue, and it was left at 4°C overnight. Needle-shaped crystals of taurine 237 were obtained.

Applicant: Nippu Suisan Co., Ltd. - Agent: Patent Attorney Yuki Ariga 1. j,, so;↓−,wa.

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Claims (1)

[Claims] 1. A method for concentrating natural taurine, which comprises removing polymeric components from stick water obtained during the production of fishmeal. 2. The method for concentrating natural taurine according to claim 1, wherein the stick water is obtained from shoji cod or/and flounder. 3. The method for concentrating natural taurine according to claim 1, wherein the stick water is obtained from sardines and/or mackerel.