CN103408473A - A method of extracting natural taurine from scallop viscera - Google Patents
A method of extracting natural taurine from scallop viscera Download PDFInfo
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- CN103408473A CN103408473A CN2013103096209A CN201310309620A CN103408473A CN 103408473 A CN103408473 A CN 103408473A CN 2013103096209 A CN2013103096209 A CN 2013103096209A CN 201310309620 A CN201310309620 A CN 201310309620A CN 103408473 A CN103408473 A CN 103408473A
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Abstract
The invention relates to a method of extracting natural taurine, and particularly relates to a method of extracting the natural taurine from scallop viscera. Specifically, the method comprises steps of drying scallops that are used as a raw material, pulverizing by using a high-speed pulverizer, adding the pulverized powder in distilled water and maintaining a constant temperature at 55-80 DEG C for 1-3 h, filtering and colleting clear liquid; adding activated carbon into the clear liquid to perform decolorization treatment, filtering and colleting clear liquid, adjusting the pH value of the clear liquid, removing acid-precipitated proteins and alkaline-precipitated proteins in the clear liquid, filtering and colleting clear liquid, concentrating the clear liquid under reduced pressure, and subjecting the concentrated liquid to purification treatment to obtain the natural taurine. The method has mild conditions, simple operation, high speed, high value and no pollution generation, and is a safe and effective method.
Description
Technical field
The present invention relates to the natural taurine extracting method, especially relate to a kind of from scallop splanchna, extracting the method for natural taurine.
Background technology
Taurine (H
2N-CH
2-CH
2SO
3H) being again Taurine, is a kind of β-sulfur-containing amino acid, because of 1872 first from oxgall, separating and gain the name.Taurine can participate in maintaining the organismic internal environment stable state, plays important regulating effect in the normal performance of the system physiological functions such as maincenter digestion reproduction immunity and internal secretion, has unique physiological property.Some developed countries have established taurine have been added in milk, strengthen its nutritive value.In addition, taurine also has anti-arrhythmia, hypotensive, antithrombotic and antiatherogenic effect.
Producing of taurine generally comprises chemical synthesis and natural goods extraction method.Adopt chemical synthesiss both at home and abroad more at present, comprise the kinds more than 10 such as girbotol process, dichloroethane law, wherein girbotol process accounting example is maximum.Taurine chemical synthesis output is high, but have that material toxicity is large, complex operation, the problems such as investment of production is high, recovery is difficult, environmental pollution.Therefore, from natural product, extracting taurine, be a kind of method safely and effectively.At present, on world market, take crude substance as the source the taurine price far above synthetics, this also provides favourable condition for the development and application of natural source taurine.Yet, due to from natural product, extracting the high cost of taurine, find the natural product that content of taurine is high and seek the focus that optimum extraction process is current research.
It is reported, in the multiple organism in ocean, the content of taurine is abundanter than terrestrial life, especially shellfish.Scallop can produce a large amount of tankage in the course of processing, wherein internal organ partly account for 17% of whole scallop weight.In the existing course of processing, scallop splanchna abandons as waste material, has caused environmental pollution and the wasting of resources.At present, from the research of extracting taurine scallop splanchna, there is not yet at home report.
Summary of the invention
It is a kind of from scallop splanchna, extracting the method for natural taurine that the object of the invention is to provide.
For achieving the above object, the technical solution used in the present invention is:
It is a kind of from scallop splanchna, extracting the method for natural taurine,
A) take scallop is raw material, with high speed disintegrator, pulverizes after oven dry, and after pulverizing, powder is in distilled water 55 ℃-80 ℃, and water bath with thermostatic control insulation 1-3h, filter and collect clear liquid, stand-by;
B) supernatant liquor of above-mentioned collection is added to the gac processing of decolouring, filter and collect clear liquid, stand-by;
C) regulate the pH value of above-mentioned collection clear liquid, then remove acidic precipitation protein and alkaline sedimentation protein in clear liquid, filter and collect clear liquid, stand-by;
D) by above-mentioned clear liquid concentrating under reduced pressure, the purified processing of gained concentrated solution is natural taurine.
Above-mentioned concentrated solution is usingd to distilled water and as elutriant, carry out the purification process of ion exchange resin absorption, obtain taurine solution; Taurine solution concentrated by rotary evaporation by gained, add dehydrated alcohol, obtains the taurine throw out, and throw out carries out crystallization and drying treatment, obtains the white crystals body of high-purity natural taurine.
Further, the tankage internal organ that produce in processing scallop process are removed to pollutent as raw material, clean up, dry, the scallop splanchna of oven dry is pulverized with high speed disintegrator; Then in 1:20-1:100(w/v) ratio join in distilled water after pulverizing, in 55 ℃ of-80 ℃ of waters bath with thermostatic control insulation 1-3h, filter and collect clear liquid, stand-by.
Further, the scallop splanchna of described oven dry is pulverized with high speed disintegrator; Then in 1:60-1:100(w/v) ratio join in distilled water after pulverizing, in 65 ℃ of-75 ℃ of waters bath with thermostatic control insulation 1.5-2.5h, filter and collect clear liquid, stand-by.
Further, the scallop splanchna of described oven dry is pulverized with high speed disintegrator; Then in 1:75-1:85(w/v) ratio join in distilled water after pulverizing, in 65 ℃ of-75 ℃ of waters bath with thermostatic control insulation 2h, filter and collect clear liquid, stand-by.
Described step c) clear liquid of above-mentioned collection is transferred to 2-4 with the precipitation acidic protein with HCl solution by pH, the centrifugal 15-25min of 4000-6000r/min, get supernatant liquor; Again to add in supernatant liquor NaOH solution regulate supernatant liquor pH be 9-11 with the precipitation basic protein, the centrifugal 15-25min of 4000-6000r/min, remove acidic precipitation protein and alkaline sedimentation protein in clear liquid, filters and collect clear liquid, stand-by.
Above-mentioned concentrated solution is usingd to distilled water and as elutriant, carry out polystyrene sodium type 732 Zeo-karbs and carry out adsorption and purification, obtain taurine solution; By the taurine solution concentrated by rotary evaporation of gained, after concentrating, add the dehydrated alcohol of concentrated solution 2-5 times volume, obtain the taurine throw out, throw out carries out crystallization and drying treatment, obtains the white crystals body of high-purity natural taurine; Wherein, elution flow rate is 2-5ml/min.
The present invention has advantages of: the present invention be take Scallop Viscus and by high speed disintegrator, is pulverized as raw material; make the cytolemma of scallop splanchna just can obtain abundant fragmentation at short notice; that taurine fully discharges when water extraction in cell; the extraction yield of taurine is improved; and then visible extracting method reaction conditions gentleness of the present invention, equipment is simple and safe, and technological operation speed is fast; can produce continuously, process in time enterprise produces in processing scallop process waste material.Both avoid environmental pollution, and can economize on resources again, turn waste into wealth, obtained the very high natural taurine of added value.Therefore promoted the benefit of industrialization extraction taurine.
The accompanying drawing explanation
The Technology Roadmap from extraction taurine scallop splanchna that Fig. 1 provides for example of the present invention.
The absorbancy of the taurine that the employing spectrophotometer method that Fig. 2 provides for example of the present invention records-concentration standard curve figure.
The specific conductivity elution curve figure recorded during column chromatography that Fig. 3 provides for example of the present invention.
The employing water extraction that Fig. 4 provides for example of the present invention when scallop splanchna, extracting taurine different feed liquid than the disparity map of the extraction yield of gained taurine.
The employing water extraction that Fig. 5 provides for example of the present invention different disparity map of extracting the extraction yield of temperature gained taurines when extracting taurine scallop splanchna.
The employing water extraction that Fig. 6 provides for example of the present invention when scallop splanchna, extracting taurine different extraction time the gained taurine the disparity map of extraction yield.
Specific embodiment
The invention will be further described below in conjunction with Figure of description, and protection scope of the present invention not only is confined to following examples.
From scallop splanchna, extracting the method for taurine, comprise the steps (referring to Fig. 1):
The tankage internal organ that produce in processing scallop process are removed to pollutent, clean up, oven dry to twice weight difference of weighing is no more than 0.002g; The scallop splanchna of oven dry is pulverized with high speed disintegrator; Get the described powder of 1.0829g and add 80ml distilled water to be placed in 75 ℃ of insulation 2h of water bath with thermostatic control, filter, get supernatant liquor; By in supernatant liquor, adding the 0.5g gac 4h that decolours, filter, get supernatant liquor; The supernatant liquor of collecting is transferred to 3 with the precipitation acidic protein with 6mol/L HCl solution by pH, then, with the centrifugal 20min of 5000r/min, gets supernatant liquor.It is 10 with the precipitation basic protein that supernatant liquor is regulated supernatant liquor pH with 5mol/L NaOH solution again, then again with the centrifugal 20min of 5000r/min, with 6mol/L HCl solution adjusting pH, is 4.5 after centrifugal treating; Remove acidic precipitation protein and alkaline sedimentation protein in clear liquid, filter and collect clear liquid, the supernatant liquor of collection is placed on Rotary Evaporators and is evaporated to 5ml; With ion exchange adsorption absorption and the purifying above-mentioned concentrated solution through concentrating under reduced pressure, specifically adopting the strongly acidic styrene is sodium type 732 Zeo-karbs, applied sample amount is 2ml, flow velocity is 3ml/min, use the distilled water wash-out, access specific conductivity from starting to drop to the elutriant (as Fig. 3) between stationary phase, obtain taurine solution; The gained taurine solution is used to the spectrophotometry absorbance under the 400nm condition, its result is consistent by the spectrophotometry result under the 400nm condition with the taurine standard substance.After typical curve (as Fig. 2) with spectrophotometry taurine standard substance, measuring content of taurine in scallop splanchna is 7.5804mg/g, and extraction yield is 0.7636%.The cut of collecting is concentrated, during gained is concentrated, add the dehydrated alcohol of 3 times of volumes, be placed in 4 ℃ of refrigerator 12h crystallization, lyophilize, obtain the white crystals body of high-purity natural taurine.
Embodiment 2
Identical with above-described embodiment operation steps; at solid-liquid ratio, be respectively 1:20,1:40:, under 1:60,1:80,1:100 condition; obtain different taurine extraction yields; the results are shown in Figure 4. as shown in Figure 4; increase along with solid-liquid ratio; the extraction yield of taurine increases, and decreases when the extraction yield of taurine can reach 6.018%, 1:100 when solid-liquid ratio is 1:80.Therefore, the more suitable solid-liquid ratio of this test is 1:80.
Embodiment 3
Identical with above-described embodiment operation steps; in the extraction temperature, be respectively under 40 ℃, 50 ℃, 60 ℃, 70 ℃, 80 ℃ conditions; obtain different taurine extraction yields; the results are shown in Figure 5. as shown in Figure 5; along with the rising of extracting temperature; the extraction yield of taurine is in rising trend in certain temperature, and this is relevant with taurine character soluble in water.Extraction yield in the time of 40 ℃ is very high, may be because inhomogeneous the causing of sampling.The extraction yield of taurine can reach 5.049% in the time of 70 ℃, decreases during to 80 ℃.The more suitable extraction temperature of this test is 70 ℃.
Embodiment 4
Identical with above-described embodiment operation steps; in extraction time, be respectively under 1.0h, 1.5h, 2.0h, 2.5h, 3.0h, condition; obtain different taurine extraction yields; the results are shown in Figure in 6. scallop splanchnas and have some enzyme; under proper condition, can make the proteolysis of self, cause taurine to decompose; thereby affect the stripping of taurine, and then have influence on the extraction yield of taurine.As shown in Figure 6, along with the prolongation of water extraction time, the extraction yield of taurine is in rising trend in 2h, and it is maximum that 2h reaches, and is that after 6.499%, 2h, extracted amount starts to descend.This test more suitable extraction time is 2h.
Embodiment 5-embodiment 13
Identical with above-described embodiment operation steps, solid-liquid ratio is 1:60, extract temperature is that 55 ℃, extraction time are 1h, and the extraction yield of taurine is 0.5569%; Solid-liquid ratio is 1:60, extract temperature is that 65 ℃, extraction time are 2h, and the extraction yield of taurine is 0.6437%; Solid-liquid ratio is 1:60, extract temperature is that 75 ℃, extraction time are 3h, and the extraction yield of taurine is 0., 3171%; Solid-liquid ratio is 1:80, extract temperature is that 55 ℃, extraction time are 2h, and the extraction yield of taurine is 0.4699%; Solid-liquid ratio is 1:80, extract temperature is that 65 ℃, extraction time are 3h, and the extraction yield of taurine is 0.5746%; Solid-liquid ratio is 1:80, extract temperature is that 75 ℃, extraction time are 1h, and the extraction yield of taurine is 0.6206%; Solid-liquid ratio is 1:100, extract temperature is that 55 ℃, extraction time are 3h, and the extraction yield of taurine is 0.4516%; Solid-liquid ratio is 1:100, extract temperature is that 65 ℃, extraction time are 1h, and the extraction yield of taurine is 0.2895%; Solid-liquid ratio is 1:100, extract temperature is that 75 ℃, extraction time are 2h, and the extraction yield of taurine is 0.6441%;
Following watch test result.
Claims (7)
1. one kind from extracting the method for natural taurine scallop splanchna, it is characterized in that:
A) take scallop is raw material, with high speed disintegrator, pulverizes after oven dry, and after pulverizing, powder is in distilled water 55 ℃-80 ℃, and water bath with thermostatic control insulation 1-3h, filter and collect clear liquid, stand-by;
B) supernatant liquor of above-mentioned collection is added to the gac processing of decolouring, filter and collect clear liquid, stand-by;
C) regulate the pH value of above-mentioned collection clear liquid, then remove acidic precipitation protein and alkaline sedimentation protein in clear liquid, filter and collect clear liquid, stand-by;
D) by above-mentioned clear liquid concentrating under reduced pressure, the purified processing of gained concentrated solution is natural taurine.
By claimed in claim 1 from scallop splanchna, extracting the method for natural taurine, it is characterized in that: above-mentioned concentrated solution is usingd to distilled water and as elutriant, carry out the purification process of ion exchange resin absorption, obtain taurine solution; Taurine solution concentrated by rotary evaporation by gained, add dehydrated alcohol, obtains the taurine throw out, and throw out carries out crystallization and drying treatment, obtains the white crystals body of high-purity natural taurine.
3. by the method from extraction natural taurine scallop splanchna claimed in claim 1, it is characterized in that: will process the tankage internal organ that produce in the scallop process and remove pollutent as raw material, clean up, dry, the scallop splanchna of oven dry is pulverized with high speed disintegrator; Then in 1:20-1:100(w/v) ratio join in distilled water after pulverizing, in 55 ℃ of-80 ℃ of waters bath with thermostatic control insulation 1-3h, filter and collect clear liquid, stand-by.
4. described from scallop splanchna, extracting the method for natural taurine by claim 1 or 3, it is characterized in that: the scallop splanchna of described oven dry is pulverized with high speed disintegrator; Then in 1:60-1:100(w/v) ratio join in distilled water after pulverizing, in 65 ℃ of-75 ℃ of waters bath with thermostatic control insulation 1.5-2.5h, filter and collect clear liquid, stand-by.
5. by the method from extraction natural taurine scallop splanchna claimed in claim 4, it is characterized in that: the scallop splanchna of described oven dry is pulverized with high speed disintegrator; Then in 1:75-1:85(w/v) ratio join in distilled water after pulverizing, in 65 ℃ of-75 ℃ of waters bath with thermostatic control insulation 2h, filter and collect clear liquid, stand-by.
6. by the method from extraction natural taurine scallop splanchna claimed in claim 1, it is characterized in that: described step c) clear liquid of above-mentioned collection is transferred to 2-4 with the precipitation acidic protein with HCl solution by pH, the centrifugal 15-25min of 4000-6000r/min, get supernatant liquor; Again to add in supernatant liquor NaOH solution regulate supernatant liquor pH be 9-11 with the precipitation basic protein, the centrifugal 15-25min of 4000-6000r/min, remove acidic precipitation protein and alkaline sedimentation protein in clear liquid, filters and collect clear liquid, stand-by.
7. described from scallop splanchna, extracting the method for natural taurine by claim 1 or 2, it is characterized in that: above-mentioned concentrated solution is usingd to distilled water and as elutriant, carry out polystyrene sodium type 732 Zeo-karbs and carry out adsorption and purification, obtain taurine solution; By the taurine solution concentrated by rotary evaporation of gained, after concentrating, add the dehydrated alcohol of concentrated solution 2-5 times volume, obtain the taurine throw out, throw out carries out crystallization and drying treatment, obtains the white crystals body of high-purity natural taurine; Wherein, elution flow rate is 2-5ml/min.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104788348A (en) * | 2015-04-30 | 2015-07-22 | 邓鑫 | Method for extracting natural taurine from pearl shells |
| CN105481734A (en) * | 2014-10-14 | 2016-04-13 | 蒲长龙 | Method for extracting taurine by using seafood leftover |
| CN105523966A (en) * | 2015-12-29 | 2016-04-27 | 广西钦州市绿源天然食品加工有限公司 | A method of extracting taurine from scallop skirt |
| CN105949088A (en) * | 2016-07-14 | 2016-09-21 | 大连民族大学 | {0><}0{>Method for extracting natural taurine from shrimps |
| CN105949089A (en) * | 2016-07-14 | 2016-09-21 | 大连民族大学 | Method for extracting natural taurine from mussels |
| CN106543043A (en) * | 2015-09-17 | 2017-03-29 | 广西正五海洋产业股份有限公司 | A kind of method that taurine is extracted in the processing byproduct from shellfish |
| CN111675637A (en) * | 2020-06-22 | 2020-09-18 | 吉林大学 | Method for preparing taurine by enzymolysis of freshwater mussel meat under assistance of high-voltage pulse electric field |
| CN113200889A (en) * | 2020-12-18 | 2021-08-03 | 浙江经贸职业技术学院 | Method for preparing taurine from mussels |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6061559A (en) * | 1983-09-14 | 1985-04-09 | Nippon Suisan Kaisha Ltd | Concentration of natural taurine |
| JPS6061558A (en) * | 1983-09-14 | 1985-04-09 | Nippon Suisan Kaisha Ltd | Production of natural taurine |
| CN1807407A (en) * | 2006-01-18 | 2006-07-26 | 厦门东海洋水产品进出口有限公司 | Method for extracting natural taurine from octopus residue using membrane separation technology |
| CN101811996A (en) * | 2010-03-01 | 2010-08-25 | 山东大学威海分校 | Preparation of taurine from squid liver |
| CN101914043A (en) * | 2010-07-23 | 2010-12-15 | 广西科学院 | Method for synchronously extracting and preparing taurine, biological zinc and polypeptide from oysters |
| CN102001974A (en) * | 2010-09-20 | 2011-04-06 | 连云港金海地食品有限公司 | Process for extracting and preparing taurine from laver |
-
2013
- 2013-07-22 CN CN201310309620.9A patent/CN103408473B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6061559A (en) * | 1983-09-14 | 1985-04-09 | Nippon Suisan Kaisha Ltd | Concentration of natural taurine |
| JPS6061558A (en) * | 1983-09-14 | 1985-04-09 | Nippon Suisan Kaisha Ltd | Production of natural taurine |
| CN1807407A (en) * | 2006-01-18 | 2006-07-26 | 厦门东海洋水产品进出口有限公司 | Method for extracting natural taurine from octopus residue using membrane separation technology |
| CN101811996A (en) * | 2010-03-01 | 2010-08-25 | 山东大学威海分校 | Preparation of taurine from squid liver |
| CN101914043A (en) * | 2010-07-23 | 2010-12-15 | 广西科学院 | Method for synchronously extracting and preparing taurine, biological zinc and polypeptide from oysters |
| CN102001974A (en) * | 2010-09-20 | 2011-04-06 | 连云港金海地食品有限公司 | Process for extracting and preparing taurine from laver |
Non-Patent Citations (4)
| Title |
|---|
| 姜新发: "翡翠贻贝中牛磺酸提取的工艺研究", 《食品科技》 * |
| 孙利芹等: "从扇贝边中提取牛磺酸工艺的研究", 《食品工业科技》 * |
| 庄淑娟，王平: "从海洋贝类中提取和分离牛磺酸的方法", 《山东理工大学学报（自然科学版）》 * |
| 杨广会等: "从鱿鱼肝脏中提取牛磺酸的工艺条件研究", 《食品科技》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105481734A (en) * | 2014-10-14 | 2016-04-13 | 蒲长龙 | Method for extracting taurine by using seafood leftover |
| CN104788348A (en) * | 2015-04-30 | 2015-07-22 | 邓鑫 | Method for extracting natural taurine from pearl shells |
| CN106543043A (en) * | 2015-09-17 | 2017-03-29 | 广西正五海洋产业股份有限公司 | A kind of method that taurine is extracted in the processing byproduct from shellfish |
| CN105523966A (en) * | 2015-12-29 | 2016-04-27 | 广西钦州市绿源天然食品加工有限公司 | A method of extracting taurine from scallop skirt |
| CN105949088A (en) * | 2016-07-14 | 2016-09-21 | 大连民族大学 | {0><}0{>Method for extracting natural taurine from shrimps |
| CN105949089A (en) * | 2016-07-14 | 2016-09-21 | 大连民族大学 | Method for extracting natural taurine from mussels |
| CN111675637A (en) * | 2020-06-22 | 2020-09-18 | 吉林大学 | Method for preparing taurine by enzymolysis of freshwater mussel meat under assistance of high-voltage pulse electric field |
| CN113200889A (en) * | 2020-12-18 | 2021-08-03 | 浙江经贸职业技术学院 | Method for preparing taurine from mussels |
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