CN103408473B - A kind of method extracting natural taurine from scallop splanchna - Google Patents
A kind of method extracting natural taurine from scallop splanchna Download PDFInfo
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- CN103408473B CN103408473B CN201310309620.9A CN201310309620A CN103408473B CN 103408473 B CN103408473 B CN 103408473B CN 201310309620 A CN201310309620 A CN 201310309620A CN 103408473 B CN103408473 B CN 103408473B
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Abstract
The present invention relates to natural taurine extracting method, especially relate to a kind of method extracting natural taurine from scallop splanchna.Particularly, be raw material with scallop, pulverize with high speed disintegrator after oven dry; powder in distilled water 55 DEG C-80 DEG C after pulverizing; water bath with thermostatic control insulation 1-3h, collecting by filtration clear liquid, adds gac by the supernatant liquor of above-mentioned collection and carries out desolventing technology; collecting by filtration clear liquid; regulate the pH value of above-mentioned collection clear liquid, then remove acidic precipitation protein and alkaline sedimentation protein in clear liquid, collecting by filtration clear liquid; by above-mentioned clear liquid concentrating under reduced pressure, the purified process of gained concentrated solution is natural taurine.Extracting method mild condition of the present invention, simple to operate, speed is fast, it is high to be worth, do not produce pollution, is one method safely and effectively.
Description
Technical field
The present invention relates to natural taurine extracting method, especially relate to a kind of method extracting natural taurine from scallop splanchna.
Background technology
Taurine (H
2n-CH
2-CH
2sO
3h) being Taurine again, is a kind of β-sulfur-containing amino acid, because 1872 separate first and gain the name from oxgall.Taurine can participate in maintaining organismic internal environment stable state, plays important regulating effect, have unique physiological property in the normal performance of the system physiological functions such as maincenter digestion reproduction immunity and internal secretion.Some developed countries have established and have been added in milk by taurine, strengthen its nutritive value.In addition, taurine also has anti-arrhythmia, hypotensive, antithrombotic and antiatherogenic effect.
Producing of taurine generally comprises chemical synthesis and Nature inorganic bone method.Adopt chemical synthesis both at home and abroad at present, comprise the kind more than 10 such as girbotol process, dichloroethane law, wherein girbotol process accounting example is maximum more.Taurine chemical synthesis output is high, but there is the problems such as material toxicity is large, complex operation, investment of production are high, recovery is difficult, environmental pollution.Therefore, from natural product, extract taurine is one method safely and effectively.At present, world market with crude substance is the taurine price in source far above synthetics, this is also for the development and application of natural source taurine provides favourable condition.But, owing to extracting the high cost of taurine from natural product, find the high natural product of content of taurine and seek the focus that optimum extraction process is current research.
It is reported, in the multiple organism in ocean, comparatively terrestrial life is abundanter for the content of taurine, especially shellfish.Scallop can produce a large amount of tankage in the course of processing, and wherein internal organ part accounts for 17% of whole scallop weight.In the existing course of processing, scallop splanchna abandons as waste material, causes environmental pollution and the wasting of resources.At present, from scallop splanchna, extracting the research of taurine, there is not been reported at home.
Summary of the invention
The object of the invention is to provide a kind of method extracting natural taurine from scallop splanchna.
For achieving the above object, the technical solution used in the present invention is:
A method for natural taurine is extracted from scallop splanchna,
A) take scallop as raw material, pulverize with high speed disintegrator after oven dry, powder in distilled water 55 DEG C-80 DEG C after pulverizing, water bath with thermostatic control insulation 1-3h, collecting by filtration clear liquid, stand-by;
B) supernatant liquor of above-mentioned collection is added gac and carry out desolventing technology, collecting by filtration clear liquid, stand-by;
C) regulate the pH value of above-mentioned collection clear liquid, then remove acidic precipitation protein and alkaline sedimentation protein in clear liquid, collecting by filtration clear liquid, stand-by;
D) by above-mentioned clear liquid concentrating under reduced pressure, the purified process of gained concentrated solution is natural taurine.
Above-mentioned concentrated solution is carried out the purification process of ion exchange resin absorption using distilled water as elutriant, obtain taurine solution; By the taurine solution concentrated by rotary evaporation of gained, add dehydrated alcohol, obtain taurine throw out, throw out carries out crystallization and drying treatment, obtains the white crystals body of high-purity natural taurine.
Further, the tankage internal organ produced in processing scallop process are removed pollutent as raw material, and clean up, dry, the scallop splanchna high speed disintegrator of oven dry is pulverized; Then in 1:20-1:100(w/v) ratio will join in distilled water after pulverizing, in 55 DEG C of-80 DEG C of waters bath with thermostatic control insulation 1-3h, collecting by filtration clear liquid, stand-by.
Further, the scallop splanchna high speed disintegrator of described oven dry is pulverized; Then in 1:60-1:100(w/v) ratio will join in distilled water after pulverizing, in 65 DEG C of-75 DEG C of waters bath with thermostatic control insulation 1.5-2.5h, collecting by filtration clear liquid, stand-by.
Further, the scallop splanchna high speed disintegrator of described oven dry is pulverized; Then in 1:75-1:85(w/v) ratio will join in distilled water after pulverizing, in 65 DEG C of-75 DEG C of waters bath with thermostatic control insulation 2h, collecting by filtration clear liquid, stand-by.
Described step c) pH is transferred to 2-4 to precipitate acidic protein by the clear liquid HCl solution of above-mentioned collection, the centrifugal 15-25min of 4000-6000r/min, gets supernatant liquor; Add in supernatant liquor again NaOH solution regulate supernatant liquor pH be 9-11 with precipitating basic protein, the centrifugal 15-25min of 4000-6000r/min, removes acidic precipitation protein and alkaline sedimentation protein in clear liquid, collecting by filtration clear liquid, stand-by.
Above-mentioned concentrated solution is carried out polystyrene sodium form 732 Zeo-karb using distilled water as elutriant and carry out adsorption and purification, obtain taurine solution; By the taurine solution concentrated by rotary evaporation of gained, add the dehydrated alcohol of concentrated solution 2-5 times volume after concentrated, obtain taurine throw out, throw out carries out crystallization and drying treatment, obtains the white crystals body of high-purity natural taurine; Wherein, elution flow rate is 2-5ml/min.
The advantage that the present invention has: the present invention is that raw material is pulverized by high speed disintegrator with Scallop Viscus; make the cytolemma of scallop splanchna just can obtain abundant fragmentation at short notice; that taurine fully discharges in cell when water extraction; the extraction yield of taurine is improved; and then visible extracting method reaction conditions of the present invention is gentle, equipment is simple and safe, and technological operation speed is fast; can continuous seepage, the waste material that the enterprise of process in time produces in processing scallop process.Both avoid environmental pollution, and can economize on resources again, turn waste into wealth, obtain the natural taurine that added value is very high.Therefore the benefit that taurine is extracted in industrialization is enhanced.
Accompanying drawing explanation
The Technology Roadmap extracting taurine from scallop splanchna that Fig. 1 provides for example of the present invention.
Absorbancy-concentration standard curve the figure of the taurine that the employing spectrophotometer method that Fig. 2 provides for example of the present invention records.
The specific conductivity elution curve figure recorded during the column chromatography that Fig. 3 provides for example of the present invention.
Fig. 4 is for different feed liquid when employing water extraction that example of the present invention provides extracts taurine from scallop splanchna is than the disparity map of the extraction yield of gained taurine.
Fig. 5 is the disparity map of the extraction yield of different Extracting temperature gained taurine when employing water extraction that example of the present invention provides extracts taurine from scallop splanchna.
Fig. 6 for when employing water extraction that example of the present invention provides extracts taurine from scallop splanchna different extraction time gained taurine the disparity map of extraction yield.
Specific embodiment
Below in conjunction with Figure of description, the invention will be further described, and protection scope of the present invention is not only confined to following examples.
Embodiment 1
From scallop splanchna, extract the method for taurine, comprise the steps (see Fig. 1):
The tankage internal organ that will produce in processing scallop process remove pollutent, clean up, and dry to twice weight difference of weighing and are no more than 0.002g; The scallop splanchna high speed disintegrator of oven dry is pulverized; The powder got described in 1.0829g adds 80ml distilled water and is placed in water bath with thermostatic control 75 DEG C insulation 2h, filters, gets supernatant liquor; Carrying out decolouring 4h by adding 0.5g gac in supernatant liquor, filtering, getting supernatant liquor; PH is transferred to 3 to precipitate acidic protein by the supernatant liquor 6mol/LHCl solution collected, and then with the centrifugal 20min of 5000r/min, gets supernatant liquor.Supernatant liquor regulates supernatant liquor pH to be 10 with precipitating basic protein with 5mol/LNaOH solution again, then again with the centrifugal 20min of 5000r/min, is 4.5 with 6mol/LHCl solution adjustment pH after centrifugal treating; Remove acidic precipitation protein and alkaline sedimentation protein in clear liquid, collecting by filtration clear liquid, is placed in the supernatant liquor of collection on Rotary Evaporators and is evaporated to 5ml; With ion exchange adsorption absorption and purifying through the above-mentioned concentrated solution of concentrating under reduced pressure, specifically adopt strongly acidic styrene system sodium form 732 Zeo-karb, applied sample amount is 2ml, flow velocity is 3ml/min, use distilled water wash-out, access specific conductivity from drop to elutriant (as Fig. 3) between stationary phase, obtain taurine solution; Gained taurine solution is used spectrophotometry absorbance under 400nm condition, and its result and taurine standard substance are consistent by spectrophotometry result under 400nm condition.After the typical curve (as Fig. 2) of spectrophotometry taurine standard substance, measuring content of taurine in scallop splanchna is 7.5804mg/g, and extraction yield is 0.7636%.The cut of collection is concentrated, during gained concentrates, adds the dehydrated alcohol of 3 times of volumes, be placed in 4 DEG C of refrigerator 12h crystallization, lyophilize, obtain the white crystals body of high-purity natural taurine.
Embodiment 2
Identical with above-described embodiment operation steps; be respectively 1:20,1:40 at solid-liquid ratio:, under 1:60,1:80,1:100 condition; obtain different taurine extraction yields; the results are shown in Figure 4. as shown in Figure 4; along with the increase of solid-liquid ratio; the extraction yield of taurine increases, and decreases when when solid-liquid ratio is 1:80, the extraction yield of taurine can reach 6.018%, 1:100.Therefore, the solid-liquid ratio that this test is comparatively suitable is 1:80.
Embodiment 3
Identical with above-described embodiment operation steps; under Extracting temperature is respectively 40 DEG C, 50 DEG C, 60 DEG C, 70 DEG C, 80 DEG C conditions; obtain different taurine extraction yields; the results are shown in Figure 5. as shown in Figure 5; along with the rising of Extracting temperature; the extraction yield of taurine is in rising trend in certain temperature, and this character soluble in water with taurine is relevant.Extraction yield 40 DEG C time is very high, may be because the uneven of sampling is caused.70 DEG C time, the extraction yield of taurine can reach 5.049%, decreases to when 80 DEG C.The Extracting temperature that this test is comparatively suitable for is 70 DEG C.
Embodiment 4
Identical with above-described embodiment operation steps; under extraction time is respectively 1.0h, 1.5h, 2.0h, 2.5h, 3.0h, condition; obtain different taurine extraction yields; the results are shown in Figure in 6. scallop splanchnas and there is some enzyme; under proper condition, the proteolysis of self can be made, cause taurine to decompose; thus affect the stripping of taurine, and then have influence on the extraction yield of taurine.As shown in Figure 6, along with the prolongation of water extraction time, the extraction yield of taurine is in rising trend in 2h, and 2h reaches maximum, is that after 6.499%, 2h, extracted amount starts to decline.The extraction time that this test is comparatively suitable for is 2h.
Embodiment 5-embodiment 13
Identical with above-described embodiment operation steps, solid-liquid ratio is 1:60, Extracting temperature is 55 DEG C, extraction time is 1h, and the extraction yield of taurine is 0.5569%; Solid-liquid ratio is 1:60, Extracting temperature is 65 DEG C, extraction time is 2h, and the extraction yield of taurine is 0.6437%; Solid-liquid ratio is 1:60, Extracting temperature is 75 DEG C, extraction time is 3h, and the extraction yield of taurine is 0., 3171%; Solid-liquid ratio is 1:80, Extracting temperature is 55 DEG C, extraction time is 2h, and the extraction yield of taurine is 0.4699%; Solid-liquid ratio is 1:80, Extracting temperature is 65 DEG C, extraction time is 3h, and the extraction yield of taurine is 0.5746%; Solid-liquid ratio is 1:80, Extracting temperature is 75 DEG C, extraction time is 1h, and the extraction yield of taurine is 0.6206%; Solid-liquid ratio is 1:100, Extracting temperature is 55 DEG C, extraction time is 3h, and the extraction yield of taurine is 0.4516%; Solid-liquid ratio is 1:100, Extracting temperature is 65 DEG C, extraction time is 1h, and the extraction yield of taurine is 0.2895%; Solid-liquid ratio is 1:100, Extracting temperature is 75 DEG C, extraction time is 2h, and the extraction yield of taurine is 0.6441%;
Following watch test result.
Claims (5)
1. from scallop splanchna, extract a method for natural taurine, it is characterized in that:
A) take scallop as raw material, pulverize with high speed disintegrator after oven dry, powder in distilled water 55 DEG C-80 DEG C after pulverizing, water bath with thermostatic control insulation 1-3h, collecting by filtration clear liquid, stand-by;
B) supernatant liquor of above-mentioned collection is added gac and carry out desolventing technology, collecting by filtration clear liquid, stand-by;
C) regulate the pH value of above-mentioned collection clear liquid, then remove acidic precipitation protein and alkaline sedimentation protein in clear liquid, collecting by filtration clear liquid, stand-by;
D) by above-mentioned clear liquid concentrating under reduced pressure, the purified process of gained concentrated solution is natural taurine;
Specific experiment is operating as: the tankage internal organ produced in processing scallop process are removed pollutent as raw material, and clean up, dry, the scallop splanchna high speed disintegrator of oven dry is pulverized; Then will join in distilled water after pulverizing in the ratio of 1:20-1:100 (w/v), in 55 DEG C of-80 DEG C of waters bath with thermostatic control insulation 1-3h, collecting by filtration clear liquid, stand-by;
Described step c) pH is transferred to 2-4 to precipitate acidic protein by the clear liquid HCl solution of above-mentioned collection, the centrifugal 15-25min of 4000-6000r/min, gets supernatant liquor; Add in supernatant liquor again NaOH solution regulate supernatant liquor pH be 9-11 with precipitating basic protein, the centrifugal 15-25min of 4000-6000r/min, removes acidic precipitation protein and alkaline sedimentation protein in clear liquid, collecting by filtration clear liquid, stand-by.
2. by the method extracting natural taurine from scallop splanchna according to claim 1, it is characterized in that: the purification process of above-mentioned concentrated solution being carried out ion exchange resin absorption using distilled water as elutriant, obtains taurine solution; By the taurine solution concentrated by rotary evaporation of gained, add dehydrated alcohol, obtain taurine throw out, throw out carries out crystallization and drying treatment, obtains the white crystals body of high-purity natural taurine.
3. by the method extracting natural taurine from scallop splanchna according to claim 1, it is characterized in that: the scallop splanchna high speed disintegrator of described oven dry is pulverized; Then will join in distilled water after pulverizing in the ratio of 1:60-1:100 (w/v), in 65 DEG C of-75 DEG C of waters bath with thermostatic control insulation 1.5-2.5h, collecting by filtration clear liquid, stand-by.
4. by the method extracting natural taurine from scallop splanchna according to claim 3, it is characterized in that: the scallop splanchna high speed disintegrator of described oven dry is pulverized; Then will join in distilled water after pulverizing in the ratio of 1:75-1:85 (w/v), in 65 DEG C of-75 DEG C of waters bath with thermostatic control insulation 2h, collecting by filtration clear liquid, stand-by.
5. by the method extracting natural taurine from scallop splanchna described in claim 1 or 2, it is characterized in that: above-mentioned concentrated solution is carried out polystyrene sodium form 732 Zeo-karb using distilled water as elutriant and carry out adsorption and purification, obtain taurine solution; By the taurine solution concentrated by rotary evaporation of gained, add the dehydrated alcohol of concentrated solution 2-5 times volume after concentrated, obtain taurine throw out, throw out carries out crystallization and drying treatment, obtains the white crystals body of high-purity natural taurine; Wherein, elution flow rate is 2-5ml/min.
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| CN105481734A (en) * | 2014-10-14 | 2016-04-13 | 蒲长龙 | Method for extracting taurine by using seafood leftover |
| CN104788348A (en) * | 2015-04-30 | 2015-07-22 | 邓鑫 | Method for extracting natural taurine from pearl shells |
| CN106543043A (en) * | 2015-09-17 | 2017-03-29 | 广西正五海洋产业股份有限公司 | A kind of method that taurine is extracted in the processing byproduct from shellfish |
| CN105523966A (en) * | 2015-12-29 | 2016-04-27 | 广西钦州市绿源天然食品加工有限公司 | A method of extracting taurine from scallop skirt |
| CN105949089B (en) * | 2016-07-14 | 2018-09-14 | 大连民族大学 | The method that natural taurine is extracted from mussel |
| CN105949088B (en) * | 2016-07-14 | 2018-09-14 | 大连民族大学 | The method that natural taurine is extracted from shrimp |
| CN111675637B (en) * | 2020-06-22 | 2022-02-08 | 吉林大学 | Method for preparing taurine by enzymolysis of freshwater mussel meat under assistance of high-voltage pulse electric field |
| CN113200889A (en) * | 2020-12-18 | 2021-08-03 | 浙江经贸职业技术学院 | Method for preparing taurine from mussels |
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