CN101811996A - Preparation of taurine from squid liver - Google Patents
Preparation of taurine from squid liver Download PDFInfo
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- CN101811996A CN101811996A CN 201010134703 CN201010134703A CN101811996A CN 101811996 A CN101811996 A CN 101811996A CN 201010134703 CN201010134703 CN 201010134703 CN 201010134703 A CN201010134703 A CN 201010134703A CN 101811996 A CN101811996 A CN 101811996A
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- taurine
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Abstract
The invention relates to preparation of taurine from a squid liver, and belongs to the technical field of research and development of medicinal products. The preparation comprises the following steps of: putting homogenate liquid of the fresh squid liver in a water bath kettle with the temperature of 55 DEG C for 20 to 24 hours; putting the homogenate liquid in a water bath kettle with the temperature of 80 DEG C to perform extraction for 50 to 60 minutes and then performing ultrasonic processing for 1 to 1.5 hours, wherein the temperature is 30 DEG C and the power is 80 W; adjusting the pH value of the filtrate and then performing centrifugation to remove protein; adding active carbon to perform adsorption for 6 hours and then filtering; adjusting the pH value of the filtrate to be 4.5 by using hydrochloric acid; allowing the filtrate to pass through 732-strong acid type cation exchange resin; according to the comparison of a Rf value of the taurine in a reference substance with that in a thin-layer chromatograph, combining the consistent part of the Rf value in the reference substance and the thin-layer chromatograph; adding absolute ethanol of which the amount is 3 times that of the concentrated solution, and putting the mixture in a refrigerator with the temperature of 4 DEG C for 12 hours; separating taurine crystal out; and performing recrystallization on the absolute ethanol to obtain the high-purity taurine crystal. The products have the functions of enhancing cell anti-oxidation, radical damage resistance, antiviral attack and the like and play an important role in vision, brain and height of a human body.
Description
Technical field
Pharmaceutical prod research and development technical field under the present invention.The present invention relates to the preparation of taurine in the squid liver.Functions such as product of the present invention has increases that cell is anti-oxidant, Green Tea Extract damage and antiviral infringement are also playing an important role aspect human body eyesight, brain and the height.
Background technology
In the last few years, along with developing rapidly of ocean-going fishing industry, squid had become one of main processing of aquatic products raw material of China.Chinese Marine Biology Resources is abundant, processing of aquatic products already has a long history, but people only pay attention to the processing to its edible part for a long time, most fishery products waste is dropped, not only caused the waste of resource, and bring comparatively serious environmental pollution, therefore solve the blue wasting of resources and blue ambient and pollute extremely urgent.
Taurine is to be separated from the bile of ox by Tidman and Gmelin in 1827, so have another name called taurocholic acid, Taurine, chemistry is by name: 2-aminoethyl sulfonic acid (2-aminoethylsulfonic acid), molecular formula: H
2NCH
2CH
2SO
3H.Taurine extensively is present in the animal tissues with unbound state; wherein content is very abundant in fish, shellfish; mollusk more so; can participate in keeping the organismic internal environment stable state; in the normal performance of system's physiological functions such as Zhong Qu, digestion, reproduction, immunity and internal secretion, play important regulatory role; in addition, taurine also has anti-arrhythmia, hypotensive, antithrombotic and antiatherogenic effect.The additional deficiency of taurine will make growing of child delay.
In Japan, squid liver, scallop enteraden the like waste almost account for half of whole Hokkaido fishery products waste, the tankage that the squid liver produces when being the processing squid, account for about 15% of squid weight in wet base, because the effect of visceral enzym in the squid viscera raw material, protein can be hydrolyzed gradually in put procedure; The meat of comparing, taurine in the squid liver, L-glutamic acid, succinate, L-Ala equal size are high.
At present, extracting the rarely seen report of research of taurine from the squid liver, is raw material with the squid liver, optimizes the technology of therefrom extracting taurine, effectively the squid waste is carried out high-valued comprehensive utilization, has practical value.
Product of the present invention is one of indispensable amino acid of wanting of body weight for humans.Functions such as it has increases that cell is anti-oxidant, Green Tea Extract damage and antiviral infringement also have crucial effect in human body eyesight, brain and height etc.Utilize cheap squid raw material to extract the relatively costly taurine of price and have higher economic value.
Summary of the invention
1, a kind of is taurine in the feedstock production squid liver with the squid liver, it is characterized in that may further comprise the steps:
Step (one): new Fresh squid liver is cleaned, and homogenate is got squid liver homogenate liquid and is placed 20-24h in 55 ℃ of water-baths;
Step (two): the homogenate in the step () is added water-bath heating that 6 times of water place 80 ℃ extract ultrasonic 1-1.5h behind the 50-60min, temperature is 30 ℃, and power is 80W, and with 4 layers of filtered through gauze, filter residue repeats to extract once, filtration;
Step (three): the filtrate in the step (two) is merged, concentrate, to 3-4, the centrifugal 20min of 4500r/min removes acidic protein, regulates pH value to 10 with sodium hydroxide again with the salt acid for adjusting pH value, the centrifugal basic protein that removes;
Step (four): the filtrate in the step (three) is added proper amount of active carbon absorption 6h after-filtration, regulate filtrate pH value to 4.5 with hydrochloric acid;
Step (five): the filtrate in the step (four) is crossed the 732-strongly acidic cation-exchange, according to the R that receives liquid thin-layer chromatography and reference substance taurine
fValue is compared, and merges R
fBe worth the part consistent, concentrate with reference substance;
Step (six): the concentrated solution in the step (five) is added the dehydrated alcohol of 3 letter amounts, place 12h in 4 ℃ of refrigerators, can separate out the taurine crystal;
Step (seven): the taurine crystal in the step (six) is added the suitable quantity of water dissolving, add dehydrated alcohol again, repeated multiple times can obtain the higher needle-like taurine crystal of purity, and its extraction yield can reach 0.67%-0.70%.
2, preparation technology according to claim 1, the raw material of taurine is the liver of Chinese Calamary Loligochinensis in the preparation squid liver.
According to the present invention, " % " among the present invention is weight percent.
Embodiment
The preparation of taurine comprises following examples in the squid liver of the present invention, and the following examples can further specify the present invention, but do not limit the present invention in any way.
Embodiment 1:
Step (one): fresh 100g squid liver is cleaned homogenate.Get squid liver homogenate liquid and place 20h in 55 ℃ of water-baths;
Step (two): the homogenate in the step () is added water-bath heating that 6 times of water place 80 ℃ extract ultrasonic 1h behind the 50min, temperature is 30 ℃, and power is 80W, and with 4 layers of filtered through gauze, filter residue repeats to extract once, filtration;
Step (three): the filtrate in the step (two) is merged, concentrate, with salt acid for adjusting pH value to 3, the centrifugal 20min of 4500r/min removes acidic protein, regulates pH value to 10 with sodium hydroxide again, the centrifugal basic protein that removes;
Step (four): the filtrate in the step (three) is added proper amount of active carbon absorption 6h after-filtration, regulate filtrate pH value to 4.5 with hydrochloric acid;
Step (five): the filtrate in the step (four) is crossed the 732-strongly acidic cation-exchange, according to the R that receives liquid thin-layer chromatography and reference substance taurine
fValue is compared, and merges R
fBe worth the part consistent, concentrate with reference substance;
Step (six): the dehydrated alcohol with 3 times of amounts of the adding of the concentrated solution in the step (five), place 12h in 4 ℃ of refrigerators, can separate out the taurine crystal;
Step (seven): the taurine crystal in the step (six) is added the suitable quantity of water dissolving, add dehydrated alcohol again, repeated multiple times can obtain the higher needle-like taurine crystal of purity, and its extraction yield can reach 0.67%.
Embodiment 2:
Step (one): fresh 100g squid liver is cleaned, and homogenate is got squid liver homogenate liquid and is placed 24h in 55 ℃ of water-baths;
Step (two): the homogenate in the step () is added water-bath heating that 6 times of water place 80 ℃ extract ultrasonic 1.5h behind the 60min, temperature is 30 ℃, and power is 80W, and with 4 layers of filtered through gauze, filter residue repeats to extract once, filtration;
Step (three): the filtrate in the step (two) is merged, concentrate, with salt acid for adjusting pH value to 4, the centrifugal 20min of 4500r/min removes acidic protein, regulates pH value to 10 with sodium hydroxide again, the centrifugal basic protein that removes;
Step (four): the filtrate in the step (three) is added proper amount of active carbon absorption 6h after-filtration, regulate filtrate pH value to 4.5 with hydrochloric acid;
Step (five): the filtrate in the step (four) is crossed the 732-strongly acidic cation-exchange, according to the R that receives liquid thin-layer chromatography and reference substance taurine
fValue is compared, and merges R
fBe worth the part consistent, concentrate with reference substance;
Step (six): the dehydrated alcohol with 3 times of amounts of the adding of the concentrated solution in the step (five), place 12h in 4 ℃ of refrigerators, can separate out the taurine crystal;
Step (seven): the taurine crystal in the step (six) is added the suitable quantity of water dissolving, add dehydrated alcohol again, repeated multiple times can obtain the higher needle-like taurine crystal of purity, and its extraction yield can reach 0.68%.
Infrared spectra is identified the taurine structure
By the infrared spectrogram of taurine reference substance as can be known: the N-H stretching vibration goes out the peak position at 3047cm-1 and 3213cm-1 place, and the C-H stretching vibration goes out the peak at the 2969cm-1 place, 1181cm
-1, 1213cm
-1, 1511cm
-1, 1585cm
-1And 1616cm
-1Be the characteristic peak of C-S and S=O, the taurine sample that the experiment separation obtains goes out the peak position and all matches with the taurine reference substance.
Description of drawings
Fig. 1 is taurine reference substance Fourier infrared spectrum figure;
Fig. 2 is that experiment separates the taurine sample Fourier infrared spectrum figure that obtains.
Claims (2)
1. one kind is taurine in the feedstock production squid liver with the squid liver, it is characterized in that may further comprise the steps:
Step (one): new Fresh squid liver is cleaned, and homogenate is got squid liver homogenate liquid and is placed 20-24h in 55 ℃ of water-baths;
Step (two): the homogenate in the step () is added water-bath heating that 6 times of water place 80 ℃ extract ultrasonic 1-1.5h behind the 50-60min, temperature is 30 ℃, and power is 80W, and with 4 layers of filtered through gauze, filter residue repeats to extract once, filtration;
Step (three): the filtrate in the step (two) is merged, concentrate, to 3-4, the centrifugal 20min of 4500r/min removes acidic protein, regulates pH value to 10 with sodium hydroxide again with the salt acid for adjusting pH value, the centrifugal basic protein that removes;
Step (four): the filtrate in the step (three) is added proper amount of active carbon absorption 6h after-filtration, regulate filtrate pH value to 4.5 with hydrochloric acid;
Step (five): the filtrate in the step (four) is crossed the 732-strongly acidic cation-exchange, according to the R that receives liquid thin-layer chromatography and reference substance taurine
fValue is compared, and merges R
fBe worth the part consistent, concentrate with reference substance;
Step (six): the dehydrated alcohol with 3 times of amounts of the adding of the concentrated solution in the step (five), place 12h in 4 ℃ of refrigerators, can separate out the taurine crystal;
Step (seven): the taurine crystal in the step (six) is added the suitable quantity of water dissolving, add dehydrated alcohol again, repeated multiple times can obtain the higher needle-like taurine crystal of purity, and its extraction yield can reach 0.67%-0.70%.
2. preparation technology according to claim 1, the raw material of taurine is the liver of Chinese Calamary Loligochinensis in the preparation squid liver.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010134703 CN101811996A (en) | 2010-03-01 | 2010-03-01 | Preparation of taurine from squid liver |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010134703 CN101811996A (en) | 2010-03-01 | 2010-03-01 | Preparation of taurine from squid liver |
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| CN101811996A true CN101811996A (en) | 2010-08-25 |
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| CN 201010134703 Pending CN101811996A (en) | 2010-03-01 | 2010-03-01 | Preparation of taurine from squid liver |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103408473A (en) * | 2013-07-22 | 2013-11-27 | 中国科学院海洋研究所 | A method of extracting natural taurine from scallop viscera |
| CN109761861A (en) * | 2018-12-29 | 2019-05-17 | 河北农业大学 | A method of extracting taurine from octopus liver |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5973561A (en) * | 1982-10-20 | 1984-04-25 | Snow Brand Milk Prod Co Ltd | Preparation of natural taurine |
| JPS6452750A (en) * | 1987-08-24 | 1989-02-28 | Yamaki Kk | Separation and purification of taurine from extract of fishes and shellfishes |
-
2010
- 2010-03-01 CN CN 201010134703 patent/CN101811996A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5973561A (en) * | 1982-10-20 | 1984-04-25 | Snow Brand Milk Prod Co Ltd | Preparation of natural taurine |
| JPS6452750A (en) * | 1987-08-24 | 1989-02-28 | Yamaki Kk | Separation and purification of taurine from extract of fishes and shellfishes |
Non-Patent Citations (1)
| Title |
|---|
| 《食品科学》 19991231 李珊 等 牛磺酸的提制 第50页第1段至51页第1.2.5 1-2 , 第10期 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103408473A (en) * | 2013-07-22 | 2013-11-27 | 中国科学院海洋研究所 | A method of extracting natural taurine from scallop viscera |
| CN103408473B (en) * | 2013-07-22 | 2016-01-06 | 中国科学院海洋研究所 | A kind of method extracting natural taurine from scallop splanchna |
| CN109761861A (en) * | 2018-12-29 | 2019-05-17 | 河北农业大学 | A method of extracting taurine from octopus liver |
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Application publication date: 20100825 |