CN103601655A - Method for extracting natural taurine from squid viscera - Google Patents

Abstract

A disclosed method for extracting natural taurine from squid viscera comprises the following steps: performing homogenate on cleaned squid viscera, performing enzymatic hydrolysis on the homonogenate, filtering and collecting the supernatant, concentrating the supernatant to precipitate protein, utilizing cation exchange resin to isolate and purify taurine, performing crystallization on the eluate to obtain taurine crystal. The method is simple and applicable to large-scale production, and extracted taurine has high purity.

Description

From squid viscera, extract the method for natural taurine

Technical field

The present invention relates to the extracting method of natural taurine, be specifically related to extract the method for natural taurine from scallop and tankage thereof.

Background technology

Taurine is a kind of sulfur-containing amino acid, chemistry 2-aminoethyl sulfonic acid by name.Taurine can extract from natural goods, also can chemosynthesis, but exist synthesis material toxicity large, and complex operation, investment of production equipment is high, reclaims difficulty, the problems such as environmental pollution.Therefore, from natural goods, extract for a long time taurine is valued by the people always.From oxgall, extracting taurine, although content is high, is not to exist with free form; In fishery products, taurine exists with free form, can extract needle crystal thing, in world market, gains great popularity.Squid is in the process of processing, and the ratio that the internal organ of squid account for squid is approximately 10%.China's squid process for processing pattern, approximately forms the waste of up to ten thousand tons, serious environment pollution every year at present.From squid viscera, extract taurine, not only there is huge economic benefit, and can improve aquatic product manufactory and utilize insufficient caused environmental pollution because of a large amount of squid visceras.

Summary of the invention

Technical problem to be solved by this invention is to propose a kind of method of extracting natural taurine from squid viscera.

Technical scheme of the present invention is: from squid viscera, extract the method for natural taurine, comprise the following steps:

(1) raw material homogenate, puts into tissue refiner's homogenate by the squid viscera cleaning and becomes pasty state, obtains homogenate;

(2) enzymolysis, puts into constant temperature pot by homogenate, adds the water of 5-8 times of homogenate weight, and temperature is controlled at 40 ℃, adds neutral protein, reaction 10-12 hour;

(3) collect supernatant liquor, product in filtration step (2), collects supernatant liquor, and by supernatant concentration to 1/4 of original volume, obtain extracting solution;

(4) protein precipitation, with hydrochloric acid, regulate the pH value to 4.0 of extracting solution, the standing acidic protein that makes fully precipitates, then centrifugal rear removal acidic protein, then extracting solution is regulated to pH value to 10 with basic solution, the standing basic protein that makes fully precipitates, and centrifugal rear removal basic protein is separated, obtains taurine extracting solution;

(5) cross Zeo-karb separation and purification taurine, regulate taurine extracting liquid pH value to 5, adopt strong cation-exchanging resin, collect taurine elutriant;

(6) crystallization and purifying, taurine elutriant adds 3-5 times of dehydrated alcohol of concentrated solution volume after concentrated, obtains taurine dirty solution, distills, and after spraying is dry, obtains taurine xln.

The invention has the beneficial effects as follows, its extraction process of method of extracting natural taurine from squid viscera is simple, is applicable to large-scale production, extracts taurine purity high.

Embodiment

Embodiment 1

The method of extracting natural taurine from squid viscera, comprises the following steps:

(1) raw material homogenate, puts into tissue refiner's homogenate by the squid viscera cleaning and becomes pasty state, obtains homogenate;

(2) enzymolysis, puts into constant temperature pot by homogenate, adds the water of 5-8 times of homogenate weight, and temperature is controlled at 40 ℃, adds neutral protein, reaction 10-12 hour;

(3) collect supernatant liquor, product in filtration step (2), collects supernatant liquor, and by supernatant concentration to 1/4 of original volume, obtain extracting solution;

(4) protein precipitation, with hydrochloric acid, regulate the pH value to 4.0 of extracting solution, the standing acidic protein that makes fully precipitates, then centrifugal rear removal acidic protein, then extracting solution is regulated to pH value to 10 with basic solution, the standing basic protein that makes fully precipitates, and centrifugal rear removal basic protein is separated, obtains taurine extracting solution;

(5) cross Zeo-karb separation and purification taurine, regulate taurine extracting liquid pH value to 5, adopt strong cation-exchanging resin, collect taurine elutriant;

(6) crystallization and purifying, taurine elutriant adds 3-5 times of dehydrated alcohol of concentrated solution volume after concentrated, obtains taurine dirty solution, distills, and after spraying is dry, obtains taurine xln.

Claims (2)

1. from squid viscera, extract the method for natural taurine, it is characterized in that, comprise the following steps:

(1) raw material homogenate, puts into tissue refiner's homogenate by the squid viscera cleaning and becomes pasty state, obtains homogenate;

(2) enzymolysis, puts into constant temperature pot by homogenate, adds neutral protein enzymolysis;

(3) collect supernatant liquor, product in filtration step (2), collects supernatant liquor, and by supernatant concentration to 1/4 of original volume, obtain extracting solution;

(4) protein precipitation, with hydrochloric acid, regulate the pH value to 4.0 of extracting solution, the standing acidic protein that makes fully precipitates, then centrifugal rear removal acidic protein, then extracting solution is regulated to pH value to 10 with basic solution, the standing basic protein that makes fully precipitates, and centrifugal rear removal basic protein is separated, obtains taurine extracting solution;

(5) cross Zeo-karb separation and purification taurine, regulate taurine extracting liquid pH value to 5, adopt strong cation-exchanging resin, collect taurine elutriant;

(6) crystallization and purifying, taurine elutriant adds 3-5 times of dehydrated alcohol of concentrated solution volume after concentrated, obtains taurine dirty solution, distills, and after spraying is dry, obtains taurine xln.

2. the method for extracting natural taurine from squid viscera according to claim 1, is characterized in that, described step (2) is specially the water that adds 5-8 times of homogenate weight, and temperature is controlled at 40 ℃, adds neutral protein, reaction 10-12 hour.