CN106986795B - A method of extracting taurine from cattle liver - Google Patents
A method of extracting taurine from cattle liver Download PDFInfo
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- CN106986795B CN106986795B CN201710365716.5A CN201710365716A CN106986795B CN 106986795 B CN106986795 B CN 106986795B CN 201710365716 A CN201710365716 A CN 201710365716A CN 106986795 B CN106986795 B CN 106986795B
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C303/00—Preparation of esters or amides of sulfuric acids; Preparation of sulfonic acids or of their esters, halides, anhydrides or amides
- C07C303/42—Separation; Purification; Stabilisation; Use of additives
- C07C303/44—Separation; Purification
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Abstract
The present invention relates to biomedicine technical field, more particularly to a kind of method for extracting taurine from cattle liver；Include the following steps：Beef liver pretreatment, refined, removal of impurities, extraction；Extracting method using the present invention; the effective recovery rate for improving taurine in cattle liver; the extraction rate reached of taurine is to 93.6%; purity is up to 98.5% or more; significantly improve taurine quality; effectively meet the needs of existing market is to natural taurine, effectively promotes the medical value of taurine.
Description
Technical field
The present invention relates to biomedicine technical field, more particularly to a kind of method for extracting taurine from cattle liver.
Background technology
China is an Animal husbandry production big country, and position is at the forefront in the world always for raw cattle breeding amount and production, according to correlation
Data shows that livestock on hand ox head number is 10626.4 ten thousand at the end of China in 2008, and beef production is about 613.2 ten thousand tons, edible internal organs
Yield accounts for about the 10% of beef production, and wherein liver yield is about 49.1 ten thousand tons.2009, China's beef production was about 8,900,000
Ton, wherein liver yield is about 71.2 ten thousand tons, has increased by 45% on a year-on-year basis.
With the fast development of beef farming and the change of people's dietary structure, beef demand obviously increases, by-product
Produce amount also increases significantly therewith, because being influenced by traditional diet, " the improper meat of by-product " is the traditional consumption of people with
And Diet concept, so far also without much changes, the liver for the byproduct-ox butchered as raw ox is considered as low-grades, can not enter bamboo mat spread on the floor for people to sit in ancient China
Seat, few people make inquiries, and added value is very low, and a large amount of taurine is rich in cattle liver, and such as irrational utilization both wasted
Valuable resource, and environment is polluted, taurine is a kind of beta-amino acids of sulfur-bearing, molecular weight 125.15,328 DEG C of fusing point
(317 DEG C of decomposition), the rodlike white crystal of monocline prismatic, taurine is distributed widely in algae and animal body, and taurine is in animal
It is distributed mainly in vivo in some excitable tissures, such as liver, central nervous system, retina, skeletal muscle and heart tissue,
Taurine can influence digesting and assimilating, influencing protein and amino acid metabolism, influence glycometabolism, influence mineral element for body lipid
Metabolism, taurine can also promote macrophage to generate interleukins-iv, reinforce the Phagocytosis function of neutrophil leucocyte,
The non-specificity and specific immune function for improving humans and animals, are the growth-promoting factors of bifid bacterium in body enteron aisle, optimize enteron aisle
Interior bacterium group structure maintains the normal reproductive function of body, also has anti-oxidant, delaying senility function, can promote oxyhepatitis
Restore normal, can also calm, analgesia and anti-inflammatory, also have preventive and therapeutic effect to frostbite, KCN poisonings and migraines, food can be applied to
The industries such as additive, medicine, cosmetics, skin care item have higher Development volue.
At present from the point of view of China's liver processing status, liver that beef cattle generates after butchering mostly as raw material it is direct on
City, small part primary are processed into food addition auxiliary material, feed etc., not high with raw material or primary processed goods sale, price so that
Enterprise makes a profit seldom, and in China, a large amount of livestock and poultry by-products are not fully utilized and livestock and poultry by-product extraction purification technical merit falls behind
Under the premise of, exploitation internal organs biochemical product has become the new industry and main trend of animal derived product, and biochemical product
Price is relatively high, and a new synergy approach is opened for enterprise.
Invention content
The present invention is in order to solve the above technical problems, provide a kind of method for extracting taurine from cattle liver.
It is realized particular by following technical scheme：
A method of it extracting taurine from cattle liver, includes the following steps：
(1) beef liver pre-processes：The connective tissue on beef liver is removed, is minced after cleaning, the 3~6% of beef liver quality are added
After mixing 120~180min of placement thoroughly, 8~10 times of distilled water of beef liver quality is added, in 1 time heating water bath of temperature in complex enzyme 1
10~15h obtains material 1；
(2) it refines：Material 1 and protein deposition agent are mixed by 1: 0.08~0.12 mass ratio, adjust the pH value of mixture
It is 4~4.5, in 2 times 2~4h of heating water bath of temperature, obtains material 2；
(3) it cleans：Material 2 is taken out, filtrate is mixed with complex enzyme 2 by 1: 1.2~1.5 mass ratio, mixed thoroughly quiet by filtering
6~8h is set, centrifuging and taking supernatant obtains material 3 by supernatant by cation exchange resin；
(4) it extracts：Material 3 is used after being ultrasonically treated 8~12min, 3~4 times of ethyl alcohol of 3 mass of material, analysis to be crystallized is added
It after going out, then is recrystallized with absolute ethyl alcohol, drying.
The complex enzyme 1 is trypsase, cathepsin and subtilopeptidase A by 0.5~1.2: 1~1.8:
0.2~0.6 mass ratio mixing.
The temperature 1 is 85~88 DEG C.
The protein deposition agent, be by content be 20~30% sulfosalisylic acid solution and it is a concentration of 0.18~
0.22% saline solution is mixed to prepare by 0.8~1.2: 1.2~1.8 mass ratio.
2 temperature of temperature is 65~75 DEG C.
The complex enzyme 2, be by papain and aspartic protease by 0.2~0.4: 0.8~1.2 quality
Than mixing.
The centrifugation, rotating speed are 3300~3800r/min.
The ultrasound, frequency are 45~55kHz.
The drying, drying temperature are 120~140 DEG C.
The ethyl alcohol, concentration >=75%.
In conclusion the beneficial effects of the present invention are：Extracting method using the present invention, it is effective to improve in cattle liver
The recovery rate of taurine, for the extraction rate reached of taurine to 93.6%, purity significantly improves taurine product up to 98.5% or more
Matter effectively meets the needs of existing market is to natural taurine, effectively promotes the medical value of taurine.
Wherein, by the pre-treatment step to cattle liver, it is added to complex enzyme, the protein in effective hydrolyzed liver,
Effectively guarantee is provided for the separation of the taurine and protein in later stage；Using the characteristic that protein is amphiprotic substance, in acidity
It is positively charged in environment, and sulfosalisylic acid group is negatively charged, positive and negative combination, effectively so that protein obtains coagulation, other egg
White matter surface has water membrane to be separated from each other protein molecule, destroys this layer of moisture film by the salt ion of suitable concentration, makes
It obtains protein molecule and coagulative precipitation occurs, to achieve the purpose that reduce protein content；By the effect of complex enzyme 2, into one
Protein is hydrolyzed step, reduces its content, further increases the purity of taurine.
Specific implementation mode
Specific embodiments of the present invention will be described in further detail below, but the invention is not limited in these realities
Mode is applied, it is claimed to still fall within the claims in the present invention for any improvement or replacement on the present embodiment essence spirit
Range.
Embodiment 1
A method of it extracting taurine from cattle liver, includes the following steps：
(1) beef liver pre-processes：The connective tissue on beef liver is removed, is minced after cleaning, the compound of the 3% of beef liver quality is added
Enzyme 1 is mixed thoroughly after placing 120min, and 8 times of distilled water of beef liver quality is added, and the heating water bath 10h at 85 DEG C obtains material 1；
The complex enzyme 1 is trypsase, cathepsin and subtilopeptidase A by 0.5: 1: 0.2 quality
Than mixing；
(2) it refines：Will material 1 and protein deposition agent by 1: 0.08~mass ratio mix, the pH value for adjusting mixture is 4~
4.5,2~4h of heating water bath at 65 DEG C obtain material 2；
It is that 20% sulfosalisylic acid solution and a concentration of 0.18% salt are water-soluble that the protein deposition agent, which is by content,
Liquid is mixed to prepare by 0.8: 1.2 mass ratio；
(3) it cleans：Will material 2 take out, filtering, filtrate is mixed with complex enzyme 2 by 1: 1.2 mass ratio, mix thoroughly standing 6~
8h centrifuges under the rotating speed of 3300r/min, takes supernatant, by supernatant by cation exchange resin, obtains material 3；
The complex enzyme 2 is to mix papain and aspartic protease by 0.2: 0.8 mass ratio；
(4) it extracts：Material 3 is used after being ultrasonically treated 8min, 3 times of ethyl alcohol of 3 mass of material is added, after precipitation to be crystallized, then
It is recrystallized with absolute ethyl alcohol, drying.
Embodiment 2
A method of it extracting taurine from cattle liver, includes the following steps：
(1) beef liver pre-processes：The connective tissue on beef liver is removed, is minced after cleaning, the compound of the 6% of beef liver quality is added
Enzyme 1 is mixed thoroughly after placing 180min, and 10 times of distilled water of beef liver quality is added, and the heating water bath 15h at 88 DEG C obtains material 1；
The complex enzyme 1 is trypsase, cathepsin and subtilopeptidase A by 1.2: 1.8: 0.6 matter
Amount is than mixing；
(2) it refines：Will material 1 and protein deposition agent by 1: 0.12 mass ratio mix, adjust mixture pH value be 4~
4.5,2~4h of heating water bath at 75 DEG C obtain material 2；
It is that 30% sulfosalisylic acid solution and a concentration of 0.22% salt are water-soluble that the protein deposition agent, which is by content,
Liquid is mixed to prepare by 1.2: 1.8 mass ratio；
(3) it cleans：Will material 2 take out, filtering, filtrate is mixed with complex enzyme 2 by 1: 1.5 mass ratio, mix thoroughly standing 6~
8h centrifuges under the rotating speed of 3800r/min, takes supernatant, by supernatant by cation exchange resin, obtains material 3；
The complex enzyme 2 is to mix papain and aspartic protease by 0.4: 1.2 mass ratio；
(4) it extracts：Material 3 is used after being ultrasonically treated 12min, 4 times of ethyl alcohol of 3 mass of material is added, after precipitation to be crystallized, then
It is recrystallized with absolute ethyl alcohol, drying.
Embodiment 3
A method of it extracting taurine from cattle liver, includes the following steps：
(1) beef liver pre-processes：The connective tissue on beef liver is removed, is minced after cleaning, the compound of the 5% of beef liver quality is added
Enzyme 1 is mixed thoroughly after placing 150min, and 9 times of distilled water of beef liver quality is added, and the heating water bath 13h at 87 DEG C obtains material 1；
The complex enzyme 1 is trypsase, cathepsin and subtilopeptidase A by 0.8: 1.5: 0.4 matter
Amount is than mixing；
(2) it refines：Will material 1 and protein deposition agent by 1: 0.1 mass ratio mix, adjust mixture pH value be 4~
4.5,2~4h of heating water bath at 70 DEG C obtain material 2；
It is that 25% sulfosalisylic acid solution and a concentration of 0.2% salt are water-soluble that the protein deposition agent, which is by content,
Liquid is mixed to prepare by 1: 1.5 mass ratio；
(3) it cleans：Will material 2 take out, filtering, filtrate is mixed with complex enzyme 2 by 1: 1.3 mass ratio, mix thoroughly standing 6~
8h centrifuges under the rotating speed of 3500r/min, takes supernatant, by supernatant by cation exchange resin, obtains material 3；
The complex enzyme 2 is to mix papain and aspartic protease by 0.3: 1 mass ratio；
(4) it extracts：Material 3 is used after being ultrasonically treated 10min, 3.5 times of ethyl alcohol of 3 mass of material is added, after precipitation to be crystallized,
It is recrystallized again with absolute ethyl alcohol, drying.
Embodiment 4
A method of it extracting taurine from cattle liver, includes the following steps：
(1) beef liver pre-processes：The connective tissue on beef liver is removed, is minced after cleaning, the compound of the 3% of beef liver quality is added
Enzyme 1 is mixed thoroughly after placing 180min, and 8 times of distilled water of beef liver quality is added, and the heating water bath 10h at 88 DEG C obtains material 1；
The complex enzyme 1 is trypsase, cathepsin and subtilopeptidase A by 1.2: 1: 0.6 quality
Than mixing；
(2) it refines：Material 1 and protein deposition agent are mixed by 1: 0.08 mass ratio, the pH value for adjusting mixture is 4.5,
The heating water bath 2h at 75 DEG C, obtains material 2；
It is that 20% sulfosalisylic acid solution and a concentration of 0.22% salt are water-soluble that the protein deposition agent, which is by content,
Liquid is mixed to prepare by 1.2: 1.8 mass ratio；
(3) it cleans：Material 2 to be taken out, filtering is mixed filtrate with complex enzyme 2 by 1: 1.5 mass ratio, is mixed thoroughly and is stood 6h,
It is centrifuged under the rotating speed of 3300r/min, takes supernatant, by supernatant by cation exchange resin, obtain material 3；
The complex enzyme 2 is to mix papain and aspartic protease by 0.2: 1.2 mass ratio；
(4) it extracts：Material 3 is used after being ultrasonically treated 8min, 4 times of ethyl alcohol of 3 mass of material is added, after precipitation to be crystallized, then
It is recrystallized with absolute ethyl alcohol, drying.
Embodiment 5
A method of it extracting taurine from cattle liver, includes the following steps：
(1) beef liver pre-processes：The connective tissue on beef liver is removed, is minced after cleaning, the compound of the 5% of beef liver quality is added
Enzyme 1 is mixed thoroughly after placing 120min, and 10 times of distilled water of beef liver quality is added, and the heating water bath 15h at 85 DEG C obtains material 1；
The complex enzyme 1 is trypsase, cathepsin and subtilopeptidase A by 0.8: 1: 0.6 quality
Than mixing；
(2) it refines：Material 1 and protein deposition agent are mixed by 1: 0.08 mass ratio, the pH value for adjusting mixture is 4.5,
2~4h of heating water bath at 75 DEG C, obtains material 2；
It is that 20% sulfosalisylic acid solution and a concentration of 0.18% salt are water-soluble that the protein deposition agent, which is by content,
Liquid is mixed to prepare by 1.2: 1.5 mass ratio；
(3) it cleans：Material 2 to be taken out, filtering is mixed filtrate with complex enzyme 2 by 1: 1.5 mass ratio, is mixed thoroughly and is stood 8h,
It is centrifuged under the rotating speed of 3800r/min, takes supernatant, by supernatant by cation exchange resin, obtain material 3；
The complex enzyme 2 is to mix papain and aspartic protease by 0.4: 0.8 mass ratio；
(4) it extracts：Material 3 is used after being ultrasonically treated 12min, 3 times of ethyl alcohol of 3 mass of material is added, after precipitation to be crystallized, then
It is recrystallized with absolute ethyl alcohol, drying.
Experimental example
The present invention counts the recovery rate and purity of taurine respectively, with tradition to the extracting method of taurine
As a contrast, the results are shown in Table 1：
Table 1
Claims (3)
1. a kind of method for extracting taurine from cattle liver, which is characterized in that include the following steps：
(1) beef liver pre-processes：The connective tissue on beef liver is removed, is minced after cleaning, the compound of the 3~6% of beef liver quality is added
Enzyme 1, after mixing 120~180min of placement thoroughly, be added beef liver quality 8~10 times of distilled water, 1 time heating water bath of temperature 10~
15h obtains material 1；
(2) it refines：Material 1 and protein deposition agent are pressed 1:0.08~0.12 mass ratio mixing, adjust mixture pH value be 4~
4.5, in 2 times 2~4h of heating water bath of temperature, obtain material 2；
(3) it cleans：Material 2 is taken out, filtering, filtrate and complex enzyme 2 are pressed 1:1.2~1.5 mass ratio mixing, mixes standing 6 thoroughly
~8h, centrifuging and taking supernatant obtain material 3 by supernatant by cation exchange resin；
(4) it extracts：Material 3 is used after being ultrasonically treated 8~12min, 3~4 times of ethyl alcohol of 3 mass of material is added, after precipitation to be crystallized,
It is recrystallized again with absolute ethyl alcohol, drying；
The complex enzyme 1 is trypsase, cathepsin and subtilopeptidase A by 0.5~1.2:1~1.8:0.2
~0.6 mass ratio mixing；
The complex enzyme 2 is by papain and aspartic protease by 0.2~0.4:0.8~1.2 mass ratio is mixed
It closes；
The ultrasound, frequency are 45~55kHz；
The temperature 1 is 85~88 DEG C；
The protein deposition agent, be by content be 20~30% sulfosalisylic acid solution and a concentration of 0.18~0.22%
Saline solution presses 0.8~1.2:1.2~1.8 mass ratio is mixed to prepare；
The temperature 2 is 65~75 DEG C；
2. the method for extracting taurine from cattle liver as described in claim 1, which is characterized in that the centrifugation, turn
Speed is 3300~3800r/min.
3. the method for extracting taurine from cattle liver as described in claim 1, which is characterized in that the drying is dried
Dry temperature is 120~140 DEG C.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6324892A (en) * | 1986-07-16 | 1988-02-02 | Taiyo Fishery Co Ltd | Liberation of taurine |
| CN102477004A (en) * | 2010-11-22 | 2012-05-30 | 河北农业大学 | Method for extracting natural taurine from oyster by ultrafiltration technology |
| CN102807511A (en) * | 2012-06-18 | 2012-12-05 | 浙江省海洋开发研究院 | Method for extracting taurine from mussel |
| CN103601655A (en) * | 2013-10-23 | 2014-02-26 | 威海博宇食品有限公司 | Method for extracting natural taurine from squid viscera |
| CN104404094A (en) * | 2014-11-03 | 2015-03-11 | 大连工业大学 | Method for extracting taurine by use of enzymatic conversion method on the basis of clams |
-
2017
- 2017-05-22 CN CN201710365716.5A patent/CN106986795B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6324892A (en) * | 1986-07-16 | 1988-02-02 | Taiyo Fishery Co Ltd | Liberation of taurine |
| CN102477004A (en) * | 2010-11-22 | 2012-05-30 | 河北农业大学 | Method for extracting natural taurine from oyster by ultrafiltration technology |
| CN102807511A (en) * | 2012-06-18 | 2012-12-05 | 浙江省海洋开发研究院 | Method for extracting taurine from mussel |
| CN103601655A (en) * | 2013-10-23 | 2014-02-26 | 威海博宇食品有限公司 | Method for extracting natural taurine from squid viscera |
| CN104404094A (en) * | 2014-11-03 | 2015-03-11 | 大连工业大学 | Method for extracting taurine by use of enzymatic conversion method on the basis of clams |
Non-Patent Citations (3)
| Title |
|---|
| "从海水珍珠提取天然牛磺酸技术研究";石珠成等;《企业科技与发展》;20140620;第71-72页 * |
| "天然牛磺酸的提取与应用";陈秋虹等;《氨基酸和生物资源》;20110615;第33卷(第2期);第43-45页 * |
| "牛肝中牛磺酸提取纯化工艺研究";孙娜;《中国优秀硕士学位论文全文数据库 工程科技I辑》;20140415;B016-321 * |
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