JPS6324892A - Liberation of taurine - Google Patents
Liberation of taurineInfo
- Publication number
- JPS6324892A JPS6324892A JP16755486A JP16755486A JPS6324892A JP S6324892 A JPS6324892 A JP S6324892A JP 16755486 A JP16755486 A JP 16755486A JP 16755486 A JP16755486 A JP 16755486A JP S6324892 A JPS6324892 A JP S6324892A
- Authority
- JP
- Japan
- Prior art keywords
- taurine
- enterococci
- extracted
- bile
- added
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 title claims abstract description 88
- 229960003080 taurine Drugs 0.000 title claims abstract description 44
- 210000000232 gallbladder Anatomy 0.000 claims abstract description 18
- 108090000790 Enzymes Proteins 0.000 claims abstract description 16
- 102000004190 Enzymes Human genes 0.000 claims abstract description 16
- 241001465754 Metazoa Species 0.000 claims abstract description 14
- 239000003858 bile acid conjugate Substances 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 21
- 210000000941 bile Anatomy 0.000 abstract description 17
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 11
- 239000008103 glucose Substances 0.000 abstract description 11
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 abstract description 6
- 108010093096 Immobilized Enzymes Proteins 0.000 abstract description 4
- 235000015278 beef Nutrition 0.000 abstract description 4
- 230000006378 damage Effects 0.000 abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 4
- 241000283690 Bos taurus Species 0.000 abstract description 3
- 241000287828 Gallus gallus Species 0.000 abstract description 3
- 235000013330 chicken meat Nutrition 0.000 abstract description 3
- 229910052697 platinum Inorganic materials 0.000 abstract description 3
- 235000015277 pork Nutrition 0.000 abstract description 3
- 241000194032 Enterococcus faecalis Species 0.000 abstract description 2
- 241000194031 Enterococcus faecium Species 0.000 abstract description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract description 2
- 229930006000 Sucrose Natural products 0.000 abstract description 2
- 239000005720 sucrose Substances 0.000 abstract description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract 1
- 239000003613 bile acid Substances 0.000 abstract 1
- 238000012136 culture method Methods 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 230000000593 degrading effect Effects 0.000 description 4
- 241000194033 Enterococcus Species 0.000 description 3
- 238000012790 confirmation Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- JAJWGJBVLPIOOH-IZYKLYLVSA-M sodium taurocholate Chemical compound [Na+].C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(=O)NCCS([O-])(=O)=O)C)[C@@]2(C)[C@@H](O)C1 JAJWGJBVLPIOOH-IZYKLYLVSA-M 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 235000010419 agar Nutrition 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 229920001661 Chitosan Polymers 0.000 description 1
- 108010000231 Choloylglycine hydrolase Proteins 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- WBWWGRHZICKQGZ-UHFFFAOYSA-N Taurocholic acid Natural products OC1CC2CC(O)CCC2(C)C2C1C1CCC(C(CCC(=O)NCCS(O)(=O)=O)C)C1(C)C(O)C2 WBWWGRHZICKQGZ-UHFFFAOYSA-N 0.000 description 1
- 229940023476 agar Drugs 0.000 description 1
- 239000000783 alginic acid Substances 0.000 description 1
- 235000010443 alginic acid Nutrition 0.000 description 1
- 229920000615 alginic acid Polymers 0.000 description 1
- 229960001126 alginic acid Drugs 0.000 description 1
- 150000004781 alginic acids Chemical class 0.000 description 1
- 239000000679 carrageenan Substances 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 229940113118 carrageenan Drugs 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000000909 electrodialysis Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000013350 formula milk Nutrition 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- WBWWGRHZICKQGZ-GIHLXUJPSA-N taurocholic acid Chemical compound C([C@@H]1C[C@H]2O)[C@@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@@H]([C@@H](CCC(=O)NCCS(O)(=O)=O)C)[C@@]2(C)[C@H](O)C1 WBWWGRHZICKQGZ-GIHLXUJPSA-N 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、動物の胆嚢中からタウリンを遊離させる方法
、詳しくは、動物の胆嚢中にタウリン−胆汁酸抱合体と
して含有されているタウリンの遊離方法に関するもので
、本発明によれば、動物の胆嚢中から抽出した胆汁エキ
ス中から、遊離のタウリンと共にタウリン−胆汁酸抱合
体として含有されているタウリンも遊離させることがで
きる。Detailed Description of the Invention [Field of Industrial Application] The present invention relates to a method for releasing taurine from the gallbladder of an animal, and more specifically, a method for releasing taurine contained in the gallbladder of an animal as a taurine-bile acid conjugate. This invention relates to a method for releasing taurine, and according to the present invention, not only free taurine but also taurine contained as a taurine-bile acid conjugate can be released from bile extract extracted from the gallbladder of an animal.
乳児用調整乳の栄養強化等に利用される、動物の胆嚢中
に含まれているタウリンの遊離法とじては、動物の胆嚢
を水と共に加熱して該胆嚢中から胆汁エキスを抽出する
方法があり、この方法によれば、タウリンは遊離状態で
胆汁エキス中に抽出されるため、適宜な手段で胆汁エキ
ス中から容易にｆｆｉ離でき、それを精製することによ
り絹製タウリンを得ることができる。A method for releasing taurine contained in animal gallbladders, which is used for nutritional enrichment of infant formula, is to heat the animal's gallbladder with water and extract bile extract from the gallbladder. According to this method, taurine is extracted into bile extract in a free state, so ffi can be easily separated from bile extract by appropriate means, and silk taurine can be obtained by purifying it. .
しかし、上記の方法による場合、動物の胆嚢中にタウリ
ン−胆汁酸抱合体として含有されているタウリンは胆汁
エキス中に遊離状態で抽出されない。However, in the above method, taurine, which is contained as a taurine-bile acid conjugate in the gallbladder of an animal, is not extracted in a free state into the bile extract.
また、タウリン−胆汁酸抱合体として存するタウリンを
、タウリン−胆汁酸抱合体を加水分解して遊離させるに
は、化学的に、強アルカリ条件下、１−トクレーブ中に
て３時間以上加熱する必要があるが、このような激しい
条件下でタウリンを遊離させると、折角遊離したタウリ
ンが破壊されでしまう惧れがある。In addition, in order to hydrolyze and liberate taurine, which exists as a taurine-bile acid conjugate, it is chemically necessary to heat it in a 1-toclave under strong alkaline conditions for 3 hours or more. However, if taurine is released under such severe conditions, there is a risk that the released taurine will be destroyed.
また、上記のタウリン−胆汁酸抱合体からタウリンを遊
離させる方法としては、コリルグリシンヒドロラーゼ等
の酵素を用いる方法があり、この方法によれば極めて穏
和な条件下に、破壊の惧れなしにタウリンを遊離させる
ことができる。しかし、このような方法は、上記のよう
な酵素が極めて高価であるため、工業的価値に乏しい。In addition, as a method for releasing taurine from the above-mentioned taurine-bile acid conjugate, there is a method using an enzyme such as cholylglycine hydrolase. According to this method, taurine can be released under extremely mild conditions without fear of destruction. can be liberated. However, such a method has little industrial value because the enzymes mentioned above are extremely expensive.
従って、本発明の目的は、動物の胆嚢中にタウリン−胆
汁酸抱合体として含有されているタウリンを、その破壊
の惧れなしに安価に遊離できる方法を提供することにあ
る。Therefore, an object of the present invention is to provide a method that can inexpensively release taurine contained in the gallbladder of animals as a taurine-bile acid conjugate without fear of its destruction.
本発明者は、上記目的を達成するため種々検討した結果
、豚肉、牛肉及び鶏肉等から分離した腸球菌がタウリン
−胆汁酸抱合体に対する加水分解活性を有しており、こ
の腸球菌によれば、動物の胆嚢中にタウリン−胆汁酸抱
合体として含有されるタウリンを温和な条件下で容易に
遊離させ得ることを知見した。As a result of various studies to achieve the above object, the present inventor found that enterococci isolated from pork, beef, chicken, etc. have hydrolytic activity against taurine-bile acid conjugates. It was discovered that taurine, which is contained in the gallbladder of animals as a taurine-bile acid conjugate, can be easily released under mild conditions.
本発明は、上記知見に基材なされたもので、動物の胆嚢
中にタウリン−胆汁酸抱合体として含有されているタウ
リンを、腸球菌又は咳腸球菌から抽出した酵素を用いて
ｔ！離させることを特徴とするタウリンの遊離方法を提
供するものである。The present invention is based on the above findings, and uses an enzyme extracted from enterococci or cough enterococci to extract taurine, which is contained in the gallbladder of animals as a taurine-bile acid conjugate. The present invention provides a method for releasing taurine, which is characterized by releasing taurine.
以下、本発明のタウリンの遊離方法について詳述する。The method for releasing taurine of the present invention will be described in detail below.
本発明で用いる腸球菌は、ストレプトコッカスフェカリ
ス（Ｓｔｒｅ ｔｏＣｏｃｃｕｓ Ｆａｅｃａｌｉｓ）
及びストレプトコッカスフェシウム（Ｓｔｒｅ ｔｏｃ
ｏｃｃｕｓ Ｆａｅｃｉｕｍ ）が代表的なもので、こ
れらの腸球菌は、例えば、豚肉、牛肉及び鶏肉から次の
ような工程で分離できる。The enterococcus used in the present invention is Streptococcus faecalis.
and Streptococcus faecium
occus Faecium) is a typical example, and these enterococci can be isolated from, for example, pork, beef, and chicken by the following process.
コロニー
工
Ｇｒｏｗｔｈ （＋）
↓
６．５％食塩ブドウ糖 ブドウ糖寒天斜面面して、
本発明を実施するには、先ず動物の胆嚢から胆汁エキス
を常法、例えば次の方法により抽出する。Colony Growth (+) ↓ 6.5% salt glucose Glucose agar slanted side,
To carry out the present invention, first, bile extract is extracted from the gallbladder of an animal by a conventional method, for example, the following method.
牛の胆嚢１００重量部に水１００〜２００重量部を加え
、これらを９０〜１００ ’Ｃ下に１．５〜２゜０時間
加熱することにより胆汁エキスを抽出する。Bile extract is extracted by adding 100 to 200 parts by weight of water to 100 parts by weight of bovine gallbladder and heating the mixture at 90 to 100°C for 1.5 to 2°C.
次いで、上記胆汁エキス１００重量部に、１〜２［を部
のグルコースを加え、これと腸球菌の分離菌株約１白金
耳を接種し、３０〜４０°Ｃ下に２〜３日間培養する。Next, 1 to 2 parts of glucose is added to 100 parts by weight of the above bile extract, and about 1 platinum loop of an isolated strain of enterococcus is inoculated with this and cultured at 30 to 40°C for 2 to 3 days.
上記グルコースは、腸球菌の発育を良くするため加える
もので、グルコースに代えてシュクロース（砂糖）等を
加えても良い。The above glucose is added to improve the growth of enterococci, and sucrose (sugar) or the like may be added instead of glucose.
然る後、上記の培養液から限外Ｉ過、精密Ｉ過等で菌体
を除去後、電気透析、イオン変換等の通常の精製手段で
タウリンを精製することにより、精製タウリンが得られ
る。また、この際、菌体は、限外Ｉ過等により分別して
再利用できる。Thereafter, purified taurine is obtained by removing bacterial cells from the above-mentioned culture solution by ultra-I filtration, precision I-filtration, etc., and then purifying taurine by conventional purification means such as electrodialysis and ion conversion. In addition, at this time, the bacterial cells can be separated and reused by ultra-I filtration or the like.
このような本発明の方法によれば、タウリン−胆汁酸抱
合体として含有されているタウリンも容易に遊離させる
ことができるため、腸球菌を用いずに、胆汁エキスから
遊離タウリンのみを分離する方法に比して、タウリンの
収量を約１．５〜２倍以上増加させることができる。According to the method of the present invention, taurine contained as a taurine-bile acid conjugate can also be easily released, so it is a method for separating only free taurine from bile extract without using enterococci. The yield of taurine can be increased by about 1.5 to 2 times or more compared to the previous method.
また、本発明は、腸球菌を上述の如く直接用いる代わり
に、腸球菌から抽出した酵素を用いることもでき、また
この酵素又は菌体を不溶性担体等に固定化した固定化酵
素を用いることもできる。Furthermore, in the present invention, instead of directly using enterococci as described above, enzymes extracted from enterococci can be used, and an immobilized enzyme obtained by immobilizing this enzyme or bacterial cells on an insoluble carrier etc. can also be used. can.
腸球菌から酵素を抽出するには、一般的な抽出法を用い
ることができるが、例えば、ブドウ糖ブイヨン培地にて
培養後、遠心分離を行って菌体を回収し、その湿ＭＮに
対して、適当な緩衝を夜（ｐＨ７前後のリン酸カンショ
ウ液等）を１０〜２０倍量加え、菌体を懸濁する。この
懸濁液を超音波破砕機等を用いて破砕後、遠心分離し、
その上澄を透析、濃縮すると、酵素を抽出することがで
きる。To extract enzymes from enterococci, general extraction methods can be used. For example, after culturing in a glucose broth medium, centrifugation is performed to collect bacterial cells, and the wet MN is Add 10 to 20 times the volume of a suitable buffer (such as a phosphate solution with a pH of around 7) to suspend the bacterial cells. This suspension is crushed using an ultrasonic crusher, etc., and then centrifuged.
The enzyme can be extracted by dialyzing and concentrating the supernatant.
また、腸球菌から抽出した酵素を固定化するには、酵素
をグルタルアルデヒド等を用いて架橋し、セラミック膜
等に吸着固定することにより行うことができる。他に、
不溶性多ｔｌ！ｉ（に−カラギーナン、アルギン酸、寒
天、キチン、キトサン等）に、グルタルアルデヒド等を
用いて適当な条件下で固定できる。又、菌体を同様な方
法で固定化することもできる。Furthermore, the enzyme extracted from enterococci can be immobilized by crosslinking the enzyme with glutaraldehyde or the like and adsorbing and fixing it onto a ceramic membrane or the like. other,
Insoluble polytl! It can be fixed to i (carrageenan, alginic acid, agar, chitin, chitosan, etc.) using glutaraldehyde or the like under appropriate conditions. In addition, bacterial cells can also be immobilized by a similar method.
本発明において、上記の酵素又は固定化酵素を使用する
場合には、前述の如くして動物の胆嚢から抽出した胆汁
エキスに、上記酵素又は固定化酵素を胆汁エキス１００
重量部に対して１０〜２０重量部添加し、３０〜４０℃
下に４〜５時間処理すれば良く、その結果、腸球菌を用
いた場合と同様にタウリン−胆汁酸抱合体として含有さ
れるタウリンをＭＵさせることができる。In the present invention, when using the above-mentioned enzyme or immobilized enzyme, the above-mentioned enzyme or immobilized enzyme is added to the bile extract extracted from the gallbladder of the animal as described above.
Add 10 to 20 parts by weight to 30 to 40°C
As a result, taurine contained as a taurine-bile acid conjugate can be converted to MU in the same manner as when enterococci are used.
以下に本発明の効果を確認するための実験例、及び本発
明の実施例を挙げる。尚、下記実験例及び実施例におい
て用いた分離菌株は、前述した手段で牛肉から分離した
腸球菌である。Experimental examples for confirming the effects of the present invention and Examples of the present invention are listed below. The isolated bacterial strain used in the following experimental examples and examples is enterococcus isolated from beef using the method described above.
実験例１ （分離菌株がタウリン−胆汁酸抱合体分解活
性を有しているか否かの確認）
分離菌株ｌ白金耳を約０．２％タウロコール酸ナトリウ
ムを含むブドウ糖ブイヨンに接種し、３７”Ｃで２４時
間培＃、ｉ＆、遊離したタウリンの量を測定した。Experimental Example 1 (Confirmation of whether the isolated bacterial strain has taurine-bile acid conjugate degrading activity) A platinum loop of isolated bacterial strain 1 was inoculated into glucose broth containing approximately 0.2% sodium taurocholate, and 37"C The amount of taurine released in the 24-hour culture #, i & was measured.
その結果、分離した２３菌株中の１６菌株が、理論量の
６０〜８６％のタウロコール酸を分解し、タウリンと遊
離させることを確認した。As a result, it was confirmed that 16 of the 23 isolated bacterial strains decomposed 60 to 86% of the theoretical amount of taurocholic acid and released it as taurine.
実験例２ （分離菌株のタウリン−胆汁酸抱合体分解活
性が菌体外酵素によるものか否かの確認）分離菌株をブ
ドウ糖ブイヨン培地にて３７°Ｃ２４時間培養した培養
液（遠心分離、滅閉濾過を旅し菌体フリーとした）に、
０．２％の濃度になるようにタウロコール酸ナトリウム
を添加し、３７°Ｃで２４時間培養した。Experimental Example 2 (Confirmation of whether the taurine-bile acid conjugate degrading activity of the isolated strain is due to extracellular enzymes) The isolated strain was cultured in a glucose broth medium at 37°C for 24 hours. (filtered to make it free of bacteria),
Sodium taurocholate was added to a concentration of 0.2% and cultured at 37°C for 24 hours.
その結果、直接菌株を接種した時に比較すると活性は１
／３程度に減少するものの菌体外酵素の活性が認められ
た。As a result, the activity was 1 when compared to when the strain was directly inoculated.
The activity of extracellular enzymes was observed, although the activity decreased to about /3.
実験例３ （分離菌株のタウリン−胆汁酸抱合体分解活
性に対するタウリン−胆汁酸抱合体の濃度の影響につい
ての確認）
分離菌株ｌ白菌耳を、約０．２．０．４．０．８．１゜
６％タウロコール酸ナトリウムをそれぞれ含むブドウ糖
ブイヨン培地にそれぞれ接種し、それぞれ３７℃で２４
．４８．７２時間培養後の遊離したタウリンの量を測定
した。Experimental Example 3 (Confirmation of the influence of the concentration of taurine-bile acid conjugate on the taurine-bile acid conjugate degrading activity of the isolated strain) .1° Each was inoculated into a glucose broth medium containing 6% sodium taurocholate, and incubated at 37°C for 24 hours.
．． The amount of taurine released after 48.72 hours of culture was measured.
その結果、分離菌株のタウリン−胆汁酸抱合体分解活性
は、約２４時間培養で飽和に達することが判った。また
、タウリン−胆汁酸抱合体の濃度が高い程分解率は低下
するが、濃度が高いとタウリンの遊離量も増加するから
、クラリン−胆汁酸抱合体の濃度が約４〜５％の時タウ
リン−胆汁酸抱合体から分離されるタウリンの遊離量が
最大になるものと認められる。As a result, it was found that the taurine-bile acid conjugate degrading activity of the isolated strain reached saturation after about 24 hours of culture. In addition, the higher the concentration of taurine-bile acid conjugate, the lower the degradation rate, but the higher the concentration, the more taurine released, so when the concentration of clarine-bile acid conjugate is about 4 to 5%, taurine - It is observed that the amount of free taurine separated from the bile acid conjugate is maximized.
実施例１
牛の胆嚢を約ＩＫｇに水１１？Ｈ合して約２時間、９０
〜１００°Ｃ下に加熱し、胆汁エキス１．５にｇを得た
。この胆汁エキスにグルコースを１５ｇ加えて、培地と
し、これに分離菌株１白耳を接種し、３７℃２日間培養
した。Example 1 Bovine gallbladder in about Ikg of water 11? H together for about 2 hours, 90
Heated to ~100°C to obtain 1.5 g of bile extract. 15 g of glucose was added to this bile extract to prepare a medium, which was inoculated with isolated strain 1 white ear and cultured at 37° C. for 2 days.
然る後、培養液から遊離タウリンを富岳により分離し、
これを電気透析して精製タウリン４５ｇを得た。After that, free taurine was separated from the culture solution by Fugaku,
This was electrodialyzed to obtain 45 g of purified taurine.
比較例１
実施例と同様にして、胆汁エキス１．５にｇを得、この
胆汁エキスから実施例と同様にしてａ離タウリンを分離
し精製タウリン３０ｇを得た。Comparative Example 1 1.5 g of bile extract was obtained in the same manner as in the example, and a-isolated taurine was separated from this bile extract in the same manner as in the example to obtain 30 g of purified taurine.
上記実施例及び比較例における結果から、本発明による
場合、収量が約５０％増加することが！Ｊする。From the results in the above Examples and Comparative Examples, the yield can be increased by about 50% according to the present invention! Do J.
本発明によれば、動物の胆嚢中にタウリン−胆汁酸抱合
体として含有されているタウリンを、その破壊の惧れな
しにｔ！離でき従来法に比してタウリンの収量を約５０
％以上増加させ得る。According to the present invention, taurine contained in the gallbladder of animals as a taurine-bile acid conjugate can be removed without fear of its destruction. The yield of taurine has been reduced by approximately 50% compared to the conventional method.
% or more.
Claims (1)
有されているタウリンを、腸球菌又は該腸球菌から抽出
した酵素を用いて遊離させることを特徴とするタウリン
の遊離方法。(1) A method for releasing taurine, which comprises releasing taurine contained in the gallbladder of an animal as a taurine-bile acid conjugate using enterococci or an enzyme extracted from the enterococci.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16755486A JPS6324892A (en) | 1986-07-16 | 1986-07-16 | Liberation of taurine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP16755486A JPS6324892A (en) | 1986-07-16 | 1986-07-16 | Liberation of taurine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS6324892A true JPS6324892A (en) | 1988-02-02 |
Family
ID=15851874
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP16755486A Pending JPS6324892A (en) | 1986-07-16 | 1986-07-16 | Liberation of taurine |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6324892A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106986795A (en) * | 2017-05-22 | 2017-07-28 | 南宁学院 | A kind of method that taurine is extracted from cattle liver |
| CN107325027A (en) * | 2017-06-19 | 2017-11-07 | 南宁学院 | A kind of method that taurine is extracted from OX-heart |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS619036A (en) * | 1984-06-23 | 1986-01-16 | Trio Kenwood Corp | Amateur radio communication equipment |
-
1986
- 1986-07-16 JP JP16755486A patent/JPS6324892A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS619036A (en) * | 1984-06-23 | 1986-01-16 | Trio Kenwood Corp | Amateur radio communication equipment |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106986795A (en) * | 2017-05-22 | 2017-07-28 | 南宁学院 | A kind of method that taurine is extracted from cattle liver |
| CN106986795B (en) * | 2017-05-22 | 2018-08-24 | 南宁学院 | A method of extracting taurine from cattle liver |
| CN107325027A (en) * | 2017-06-19 | 2017-11-07 | 南宁学院 | A kind of method that taurine is extracted from OX-heart |
| CN107325027B (en) * | 2017-06-19 | 2019-05-14 | 南宁学院 | A method of extracting taurine from cattle heart |
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