CN106834398A - A kind of preparation technology of silkworm chrysalis active protein peptide - Google Patents
A kind of preparation technology of silkworm chrysalis active protein peptide Download PDFInfo
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- CN106834398A CN106834398A CN201510881131.XA CN201510881131A CN106834398A CN 106834398 A CN106834398 A CN 106834398A CN 201510881131 A CN201510881131 A CN 201510881131A CN 106834398 A CN106834398 A CN 106834398A
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- silkworm chrysalis
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Abstract
The invention discloses a kind of preparation technology of silkworm chrysalis active protein peptide, 1 is comprised the concrete steps that）Pretreatment, isolates silkworm chrysalis oil and protein feed；2）Enzymolysis：Enzymolysis filtering, collects filtrate, and concentration is dried, and obtains final product pupa albumen peptide.Compared with prior art, the enzymatic compositions that the present invention is constituted in specific proportions with alkali protease, neutral proteinase and flavor protease are digested to the feed liquid of fresh silkworm chrysalis under given conditions, various enzyme synergies in enzymolysis process, while degree of hydrolysis is effectively improved, obtained pupa albumen peptide is without bitter taste, comfortable taste.
Description
Technical field
The invention belongs to technical field of natural product extraction, and in particular to be a kind of preparation technology of silkworm chrysalis active protein peptide.
Background technology
Silkworm chrysalis also known as chalcid fly, butterfly unit, silkworm-raising woman, are the pupa of Bombycidae silkworm, and China's silkworm chrysalis aboundresources produces silkworm chrysalis per year more than 300,000 tons, accounts for the 80% of whole world total output.Modern study shows that silkworm chrysalis not only with nutritive value very high, also with promotion fat metabolism, protects the multiple pharmacological effects such as liver, liver cell regeneration, growth intelligence, protection eyesight, anti-aging, raising immunity function, strong, hypoglycemic and anticancer.Modern age《Dictionary of medicinal plant》、《Chinese medicinal herbal》、《Chinese medicine sea》、《Chinese medicine Ci hai》Deng being included silkworm chrysalis as Chinese medicine, it is used to treat the malnutrition illness such as infantile malnutrition due to digestive disturbances or intestinalparasites fatigue as help medicine.Therefore, silkworm chrysalis is a kind of precious resources of medicine-food two-purpose.
Fat content quite enriches in silkworm chrysalis, accounts for dry silkworm chrysalis 28%~30%, contains a large amount of Unsaturatcd fatty acid glycerides.Silkworm chrysalis oil can be hydrolyzed into various aliphatic acid, wherein unrighted acid accounts for 75%, mainly oleic acid, linoleic acid and leukotrienes etc., saturated fatty acid is mainly palmitic acid and stearic acid, due to having three α-leukotrienes of unsaturated double-bond containing a large amount of in pupal fat, therefore the degree of unsaturation of pupal fat is at a relatively high.Thick pupal fat can produce surfactant sulfonation silkworm chrysalis oil and modifying plastics additive epoxy pupal fat as the raw material of industry, also can be mixed and made into high-quality soap with other greases；Epoxy pupal fat ester is used for the processing such as plastics, film, cable, leather and nylon synthesis；Pupal fat can also make defoamer, senior lubricant, shoe polish, footwear ink additive, also can cosmetic material processed, have resisting ultraviolet radiation, make skin metabolism vigorous and soft effect.Content highest oleic acid in pupal fat, has good facilitation to growth in humans's development；And linoleic acid is the essential aliphatic acid of human body, human body will lean on linoleic acid to synthesize arachidonic acid, and then generate prostaglandin, especially obvious to reduce serum cholesterol effect to reconcile the various functions of human body.Linolenic content is very high in pupal fat, up to more than 30%.Leukotrienes is a kind of Multifunctional health product composition; it is synthesis eicosapentaenoic acid (EPA), the raw material of DHA (DHA) in human body; to increasing intelligence and memory; protection eyesight has obvious effect; also having simultaneously persistently reduces the effect of blood ester, cholesterol and anti-platelet aggregation; can also hypotensive, preventing and treating thrombosis, prevention of cardiovascular disease etc. function.Therefore, it can be said that silkworm chrysalis oil is a kind of at a relatively high special animalses oil of healthy nutritive value, and it is used as health food and the raw material of cosmetics, and prospect is also expected.
Silkworm chrysalis is rich in protein simultaneously, accounts for the 50%~60% of dry silkworm chrysalis, and predominantly high-quality globulin, is the animal protein resource and nutraceutical of ideals of human being.Pupa albumen is a kind of adequate proteins, containing 18~20 kinds of amino acid, wherein for human body essential amino acid has 8 kinds, such as tryptophan, lysine, phenylalanine, leucine, isoleucine, methionine, threonine and valine.Their content accounts for more than the 40% of amino acid, meets the amino acid pattern that FAO WHO specify.
According to analysis, calculated with protein content：100kg
Fresh pupa is equivalent to 85kg thin porks, 96kg eggs or l00kg crucians.Pupa albumen also rich in nutrients such as riboflavin, niacin, zinc, iron, copper, with the nutrition of supplement body, the effect of enhancing development, can be widely used for the nutraceutical additive and nourishing agent of infant, pregnant woman, old man, eipathia asthenia person and sportsman.Silkworm is the economic insects for easily breeding, and silkworm chrysalis is again the native protein resource of great potential.In recent years, China's pupa albumen develops research and achieves rapid progress, and the application in fields such as nutritional health food, medication chemistry, animal and fowl fodders shows good development momentum.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of degree of hydrolysis technique for preparing pupa albumen peptide with enzyme hydrolysis silkworm chrysalis higher.Silkworm chrysalis is digested using the method, degree of hydrolysis is up to more than 55%, and the pupa albumen peptide that enzymolysis is obtained is without bitter taste, comfortable taste.
For solve problem, concrete technical scheme of the present invention is as follows：
1）Pretreatment：The silkworm chrysalis of fresh survival is placed in freezer and is freezed, silkworm chrysalis and water after freezing are stirred chopping, obtain mixture in being added to blade impellers together；Gained mixture respectively obtains oil reservoir, water layer and solid layer by after sieving, filtrate uses three-phase centrifuge centrifugation, and oil reservoir is that silkworm chrysalis bio oil is used to make silkworm chrysalis oil；Solid layer and water layer are pretreatment material, the raw material for preparing pupa albumen peptide；
2）Enzymolysis：It is 7.0~8.0 that pretreatment material is adjusted into its pH, is subsequently adding enzymatic compositions, is warming up to 45~55 DEG C of insulation 4~8h of enzymolysis, and gone out enzyme, filtering, collects filtrate, and concentration is dried, and obtains final product pupa albumen peptide.
The step 2）Middle enzymatic compositions are that alkali protease, neutral proteinase and flavor protease press 1：1.5 ：Than the composition of composition, the addition of enzymatic compositions is the 3~5% of fresh silkworm chrysalis weight to 0.8 weight.
Described flavor protease is Novi trade wind taste Protease F lavourzyme, and its enzyme activity is 500LAPU/
Gram；The enzyme activity of the alkali protease is 200000U/g, and the enzyme activity of the neutral proteinase is 200000U/g.
Compared with prior art, the enzymatic compositions that the present invention is constituted in specific proportions with alkali protease, neutral proteinase and flavor protease are digested to the slurries of fresh silkworm chrysalis under given conditions, various enzyme synergies in enzymolysis process, while degree of hydrolysis is effectively improved, obtained pupa albumen peptide is without bitter taste, comfortable taste.
Specific embodiment
With specific embodiment, the invention will be further described below, but the invention is not limited in these embodiments.
Embodiment 1
1）Pretreatment：Fresh survival silkworm chrysalis is immediately placed in -5 DEG C of freezers of temperature and freezes 2h；The silkworm chrysalis that will be freezed is added in 2 times of amount pure water, and 1h is stirred with 100rpm blade impellers；The silkworm chrysalis that will be smashed is centrifuged with 40 mesh number screen filtrations, filtrate with 10000rpm three-phase centrifuges, separates to obtain light yellow oil reservoir, as silkworm chrysalis oil；Collect milky water layer and solid layer is pretreatment material, for the raw material for preparing pupa albumen peptide；
2）Enzymolysis：It is 7.0 that pretreatment material is adjusted into its pH, is subsequently adding the enzymatic compositions equivalent to fresh silkworm chrysalis weight 3%（The weight ratio of alkali protease, neutral proteinase and flavor protease is 1：1.5 ：0.8, wherein flavor protease is Novi trade wind taste Protease F lavourzyme, and its enzyme activity is 500LAPU/
Gram；The enzyme activity of alkali protease is 200000U/g, and the enzyme activity of neutral proteinase is 200000U/g.）, 45 DEG C of insulation enzymolysis 8h are warming up to, go out enzyme, filtering, collects filtrate (degree of hydrolysis is 61%), is concentrated under reduced pressure, and spray drying obtains final product pupa albumen peptide；
Gained pupa albumen peptide adds water the solution for being made into that mass concentration is 5%, without bitter taste, comfortable taste.
The detection method of sample degree of hydrolysis is in the present embodiment：Take the filtrate 5mL being collected by filtration after the enzyme that goes out and be settled to 100mL, its amino acid content is determined using the method for ninhydrin, degree of hydrolysis is calculated by following equation：
Embodiment 2
1）Pretreatment：With embodiment 1；
2）Enzymolysis：It is 8.0 that pretreatment material is adjusted into its pH, is subsequently adding the enzymatic compositions equivalent to fresh silkworm chrysalis weight 5%（The weight ratio of alkali protease, neutral proteinase and flavor protease is 1：1.5 ：0.8, wherein flavor protease is Novi trade wind taste Protease F lavourzyme, and its enzyme activity is 500LAPU/
Gram；The enzyme activity of alkali protease is 200000U/g, and the enzyme activity of neutral proteinase is 200000U/g.）, 55 DEG C of insulation enzymolysis 4h are warming up to, go out enzyme, filtering, collects filtrate (degree of hydrolysis is 60%, and detection method is with embodiment 1), is concentrated under reduced pressure, and spray drying obtains final product pupa albumen peptide；
Gained pupa albumen peptide adds water the solution for being made into that mass concentration is 5%, without bitter taste, comfortable taste.
Embodiment 3
1）Pretreatment：With embodiment 1；
2）Enzymolysis：It is 7.5 that pretreatment material is adjusted into its pH, is subsequently adding the enzymatic compositions equivalent to fresh silkworm chrysalis weight 4%（The weight ratio of alkali protease, neutral proteinase and flavor protease is 1：1.5 ：0.8, wherein flavor protease is Novi trade wind taste Protease F lavourzyme, and its enzyme activity is 500LAPU/
Gram；The enzyme activity of alkali protease is 200000U/g, and the enzyme activity of neutral proteinase is 200000U/g.）, 50 DEG C of insulation enzymolysis 6h are warming up to, go out enzyme, filtering, collects filtrate (degree of hydrolysis is 62%, and detection method is with embodiment 1), is concentrated under reduced pressure, and spray drying obtains final product pupa albumen peptide；
Gained pupa albumen peptide adds water the solution for being made into that mass concentration is 5%, without bitter taste, comfortable taste.
Claims (2)
1. a kind of preparation technology of silkworm chrysalis active protein peptide, it is characterised in that comprise the following steps：
1）Pretreatment：The silkworm chrysalis of fresh survival is placed in freezer and is freezed, silkworm chrysalis and water after freezing are stirred chopping, obtain mixture in being added to blade impellers together；Gained mixture respectively obtains oil reservoir, water layer and solid layer by after sieving, filtrate uses three-phase centrifuge centrifugation, and oil reservoir is that silkworm chrysalis bio oil is used to make silkworm chrysalis oil；Solid layer and water layer are pretreatment material, the raw material for preparing pupa albumen peptide；
2）Enzymolysis：It is 7.0~8.0 that pretreatment material is adjusted into its pH, is subsequently adding enzymatic compositions, is warming up to 45~55 DEG C of insulation 4~8h of enzymolysis, and gone out enzyme, filtering, collects filtrate, and concentration is dried, and obtains final product pupa albumen peptide.
2. the technique according to claim 1, it is characterised in that：The step 2）Middle enzymatic compositions are that alkali protease, neutral proteinase and flavor protease press 1：1.5 ：Than the composition of composition, the addition of enzymatic compositions is the 3~5% of fresh silkworm chrysalis weight to 0.8 weight；Wherein described flavor protease is Novi trade wind taste Protease F lavourzyme, and its enzyme activity is 500LAPU/
Gram；The enzyme activity of the alkali protease is 200000U/g, and the enzyme activity of the neutral proteinase is 200000U/g.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109401898A (en) * | 2018-11-01 | 2019-03-01 | 广西壮族自治区蚕业技术推广总站 | A kind of preparation method rich in silkworm chrysalis peptide and its microelement sorosis alcoholic drink mixed with fruit juice |
| CN112841405A (en) * | 2021-02-22 | 2021-05-28 | 苏州市格立尔环保技术有限公司 | Method for preparing degreased insect powder and insect oil by utilizing hermetia illucens |
-
2015
- 2015-12-07 CN CN201510881131.XA patent/CN106834398A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109401898A (en) * | 2018-11-01 | 2019-03-01 | 广西壮族自治区蚕业技术推广总站 | A kind of preparation method rich in silkworm chrysalis peptide and its microelement sorosis alcoholic drink mixed with fruit juice |
| CN112841405A (en) * | 2021-02-22 | 2021-05-28 | 苏州市格立尔环保技术有限公司 | Method for preparing degreased insect powder and insect oil by utilizing hermetia illucens |
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Application publication date: 20170613 |