CN105341315A - Protein hydrolyzate preparation technology - Google Patents
Protein hydrolyzate preparation technology Download PDFInfo
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- CN105341315A CN105341315A CN201510720776.5A CN201510720776A CN105341315A CN 105341315 A CN105341315 A CN 105341315A CN 201510720776 A CN201510720776 A CN 201510720776A CN 105341315 A CN105341315 A CN 105341315A
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- protein
- hydrolyzate
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/14—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from leguminous or other vegetable seeds; from press-cake or oil-bearing seeds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/02—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from meat
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- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
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- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention discloses a protein hydrolyzate preparation technology which includes the following steps: pre-treatment, soybean protein preparation,activation treatment of enzymes in porcine pancreas, hydrolysis reaction, hydrolyzate separation, high temperature inactivation, sedimentation treatment, concentration, secondary inactivation, secondary sedimentation, secondary separation, etc. Compared to the prior art, the porcine pancreas are directly used and subjected to lime milk treatment at high static pressure conditions, the activity of pancreatic enzymes are higher, the hydrolytic effect is good, and the protein hydrolyzate does not contain heavy metals. Besides, compound flavored protease and alkaline protease are used in the hydrolysis, the hydrolytic efficiency is high and the product is good in taste.The secondary sedimentation and separation are used to finally obtain a product with fewer impurities,protein oral liquid in a liquid state contains no suspended substances and the product quality is improved. The protein is completely hydrolyzed and the protein hydrolyzate is rich in nutrition and easy to absorb.
Description
Technical field
The present invention relates to protein hydrolysate preparing technical field, be specifically related to a kind of preparation technology of protein hydrolysate.
Background technology
Beef contains rich in protein, can improve body resistance against diseases, to grow and Post operation, aftercare people supplement lose blood, suitable especially in repair tissue etc.The traditional Chinese medical science is thought, beef has tonifying middle-Jiao and Qi, nourishes taste, strong muscles and bones, the breath wind that reduces phlegm, the raw saliva of cough-relieving effect, be applicable to the sinking of qi of middle-jiao, body void of breathing hard, soreness of bones and muscles, anaemia prolonged illness and the yellow dizzy people in face eat.Can not synthesize but essential amino acid containing abundant vitamin A, B, D, E and multiple human body in soybean, normal food soybean, skin can be made delicate, pale, moist, effectively prevent the appearance of freckle and wrinkle, high content of protein matter in soybean, can nutrition skin hair, makes human body plentiful solid, hair is pitch-black glossy, agerasia.Nutriment contained in egg is quite abundant, containing protein, phosphatide, vitamin A, vitamin B1, vitamin B2, calcium, iron, vitamin D etc., containing a certain amount of phosphatide in egg yolk, enter the choline that the phosphatide in human body is separated, have and prevent skin senescence, make skin smooth pretty and dazzling, also containing abundant iron in egg.Not yet occur both containing animal protein, vegetable protein at present, again containing egg albumen, nutritious protein hydrolysate product.
Enzymatic isolation method prepares protein hydrolysate, and because its reaction condition is gentle, amino acid destructive rate is low, can not produce racemization, thus be widelyd popularize.But the protein hydrolysate prepared through enzyme process is often with bitter taste, and its main cause is the effect of some hydrophobic groupings of protein itself.Find after deliberation, the principal element affecting bitter taste in hydrolytic process has the position of hydrophobic grouping, degree of hydrolysis and pH acid etc.At present, mainly contain adsorption filtration, shelter, the method such as selectivity enzyme and selective precipitation with removing or bitter taste in lessening hydrolytic degradation albumen, wherein adsorption filtration can only remove part bitter taste, and easily loses protein; Sheltering investment, to be only suitable for bitter peptide molecule cavity volume in those hydrolyzates little, large cannot effectively being buried of cavity volume, and bitter taste removal effect is not good; Selectivity enzyme process debitterize mainly utilizes excision enzyme to make it discharge the excision of the hydrophobic amino acid of bitter peptide, thus reduces bitter taste, and the shortcoming of this method is that the protolysate obtained is soluble, thus limits its application.
In addition, in prior art be the pancreatin that extract of direct employing to carry out the hydrolysis of protein, and pancreatin is during preservation, and activity is difficult to keep, and directly affects the degree of hydrolysis of albumen, and then affects mouthfeel and the quality of protein hydrolysate.
Summary of the invention
For the deficiency that above-mentioned prior art exists, the invention provides a kind of nutritious, without bitter off-flavors and the preparation technology of the good protein hydrolysate of protein quality.
Technical scheme of the present invention: a kind of preparation technology of protein hydrolysate, comprises following concrete steps:
(1) pre-treatment: 160 kilograms, the beef of frozen for storage under being-18 DEG C of conditions in temperature, pig pancreas 800-1400 kilogram are rubbed into sauce shape respectively, smashes 16 kilograms, egg and abandons shell and get liquid and stir, for subsequent use;
(2) the producing of soybean protein: dregs of beans first uses microwave treatment 5-8min, microwave treatment temperature is 40-55 DEG C, and microwave power is 600-800W, then adopts alkali extraction-acid precipitation to extract soybean protein from dregs of beans, during extraction, pH value is 6-6.5, and wherein the consumption of dregs of beans is 400 kilograms;
(3) enzyme activition process in pig pancreas: the milk of lime adding pig pancreas gross mass 0.1%-0.2% in the pig pancreas rubbing into sauce shape, and process 10-20min under the high hydrostatic pressure condition being 50-100Mpa in pressure size;
(4) hydrolysis: add pig pancreas, egg liquid and the soybean protein after the beef of rubbing, activation in hydrolytic decomposition pot, and add compound fertilizer production, alkali protease, enzymolysis is carried out under the adjustment of stirring, wherein the concentration of compound fertilizer production is 1000U/g, concentration of substrate is 3%, hydrolysis temperature is 55 ± 2 DEG C, hydrolysis time 9-10h;
(5) hydrolyzate is separated: the hydrolyzate after hydrolysis is sent to disk centrifuge and carries out separation and remove slag;
(6) high-temperature inactivation: the hydrolyzate after being separated is carried out inactivation treatment under 100 DEG C of conditions;
(7) settlement treatment: the hydrolyzate after deactivation is sent in settling tank, more than sedimentation 72h;
(8) concentrated: concentrate the hydrolyzate after sedimentation according to a conventional method, concentrated proportion is 1.05-1.10;
(9) secondary deactivation: the temperature of deactivation is also 100 DEG C;
(10) secondary settlement: adopt settling tank to carry out secondary settlement, sedimentation 24h;
(11) secondary separation: adopt high speed channel separator to carry out secondary separation, rotating speed is that 15000-16300 turns/min, obtains protein hydrolysate concentrate;
(12) post processing: by directly canned for the protein hydrolysate concentrate obtained after secondary separation, obtain protein hydrolysate oral liquid, or spray-dried, obtain protein hydrolysate powder.
Advantage of the present invention: compared to existing technologies, the present invention directly adopts pig pancreas, and processes under milk of lime and high hydrostatic pressure condition, and enzymatic activity is higher, and hydrolysis effect is good, and can not contain heavy metal; And adopting compound fertilizer production, alkali protease during hydrolysis, hydrolysis efficiency is high, and products taste is good; Adopt secondary settlement, secondary separation, make the final product impurity obtained few, the protein oral liquor of liquid condition, not containing suspended material, improves the quality of product.The invention enables proteolysis complete, nutritious, and easily absorb.
Detailed description of the invention
Now come in conjunction with specific embodiments to set forth the present invention further.
Embodiment 1
(1) pre-treatment: 160 kilograms, the beef of frozen for storage under being-18 DEG C of conditions in temperature, pig pancreas 800 kilograms are rubbed into sauce shape respectively, smashes 16 kilograms, egg and abandons shell and get liquid and stir, for subsequent use;
(2) the producing of soybean protein: the dregs of beans of 400 kilograms first uses microwave treatment 8min, and microwave treatment temperature is 40 DEG C, and microwave power is 600W, then adopt alkali extraction-acid precipitation to extract soybean protein from dregs of beans, during extraction, pH value is 6;
(3) enzyme activition process in pig pancreas: the milk of lime adding pig pancreas gross mass 0.1% in the pig pancreas rubbing into sauce shape, and process 20min under the high hydrostatic pressure condition being 80Mpa in pressure size;
(4) hydrolysis: add pig pancreas, egg liquid and the soybean protein after the beef of rubbing, activation in hydrolytic decomposition pot, and add compound fertilizer production, alkali protease, enzymolysis is carried out under the adjustment of stirring, wherein the concentration of compound fertilizer production is 1000U/g, concentration of substrate is 3%, hydrolysis temperature is 55 DEG C, hydrolysis time 9h;
(5) hydrolyzate is separated: the hydrolyzate after hydrolysis is sent to disk centrifuge and carries out separation and remove slag, the power of disk centrifuge is 5 tons/hour;
(6) high-temperature inactivation: the hydrolyzate after being separated is carried out inactivation treatment under 100 DEG C of conditions;
(7) settlement treatment: the hydrolyzate after deactivation is sent in settling tank, more than sedimentation 72h;
(8) concentrated: concentrate the hydrolyzate after sedimentation according to a conventional method, concentrated proportion is 1.05;
(9) secondary deactivation: the temperature of deactivation is also 100 DEG C;
(10) secondary settlement: adopt settling tank to carry out secondary settlement, sedimentation 24h;
(11) secondary separation: adopt high speed channel separator to carry out secondary separation, rotating speed is 15000 turns/min, obtains protein hydrolysate concentrate;
(12) post processing: by directly canned for the protein hydrolysate concentrate obtained after secondary separation, obtain protein hydrolysate oral liquid, or spray-dried, obtain protein hydrolysate powder.
Embodiment 2
Pre-treatment: 160 kilograms, the beef of frozen for storage under being-18 DEG C of conditions in temperature, pig pancreas 1400 kilograms are rubbed into sauce shape respectively, smashes 16 kilograms, egg and abandons shell and get liquid and stir, for subsequent use;
(2) the producing of soybean protein: 400 kilograms of dregs of beans first use microwave treatment 5-8min, and microwave treatment temperature is 40-55 DEG C, and microwave power is 600W, then adopt alkali extraction-acid precipitation to extract soybean protein from dregs of beans, during extraction, pH value is 6.5;
(3) enzyme activition process in pig pancreas: the milk of lime adding pig pancreas gross mass 0.2% in the pig pancreas rubbing into sauce shape, and process 10min under the high hydrostatic pressure condition being 100Mpa in pressure size;
(4) hydrolysis: add pig pancreas, egg liquid and the soybean protein after the beef of rubbing, activation in hydrolytic decomposition pot, and add compound fertilizer production, alkali protease, enzymolysis is carried out under the adjustment of stirring, wherein the concentration of compound fertilizer production is 1000U/g, concentration of substrate is 3%, hydrolysis temperature is 52 DEG C, hydrolysis time 10h;
(5) hydrolyzate is separated: the hydrolyzate after hydrolysis is sent to disk centrifuge and carries out separation and remove slag;
(6) high-temperature inactivation: the hydrolyzate after being separated is carried out inactivation treatment under 100 DEG C of conditions;
(7) settlement treatment: the hydrolyzate after deactivation is sent in settling tank, more than sedimentation 72h;
(8) concentrated: concentrate the hydrolyzate after sedimentation according to a conventional method, concentrated proportion is 1.05-1.10;
(9) secondary deactivation: the temperature of deactivation is also 100 DEG C;
(10) secondary settlement: adopt settling tank to carry out secondary settlement, sedimentation 24h;
(11) secondary separation: adopt high speed channel separator to carry out secondary separation, rotating speed is 16300 turns/min, obtains protein hydrolysate concentrate;
(12) post processing: by directly canned for the protein hydrolysate concentrate obtained after secondary separation, obtain protein hydrolysate oral liquid, or spray-dried, obtain protein hydrolysate powder.
Claims (1)
1. a preparation technology for protein hydrolysate, comprises following concrete steps:
(1) pre-treatment: 160 kilograms, the beef of frozen for storage under being-18 DEG C of conditions in temperature, pig pancreas 800-1400 kilogram are rubbed into sauce shape respectively, smashes 16 kilograms, egg and abandons shell and get liquid and stir, for subsequent use;
(2) the producing of soybean protein: dregs of beans first uses microwave treatment 5-8min, microwave treatment temperature is 40-55 DEG C, and microwave power is 600-800W, then adopts alkali extraction-acid precipitation to extract soybean protein from dregs of beans, during extraction, pH value is 6-6.5, and wherein the consumption of dregs of beans is 400 kilograms;
(3) enzyme activition process in pig pancreas: the milk of lime adding pig pancreas gross mass 0.1%-0.2% in the pig pancreas rubbing into sauce shape, and process 10-20min under the high hydrostatic pressure condition being 50-100Mpa in pressure size;
(4) hydrolysis: add pig pancreas, egg liquid and the soybean protein after the beef of rubbing, activation in hydrolytic decomposition pot, and add compound fertilizer production, alkali protease, enzymolysis is carried out under the adjustment of stirring, wherein the concentration of compound fertilizer production is 1000U/g, concentration of substrate is 3%, hydrolysis temperature is 55 ± 2 DEG C, hydrolysis time 9-10h;
(5) hydrolyzate is separated: the hydrolyzate after hydrolysis is sent to disk centrifuge and carries out separation and remove slag;
(6) high-temperature inactivation: the hydrolyzate after being separated is carried out inactivation treatment under 100 DEG C of conditions;
(7) settlement treatment: the hydrolyzate after deactivation is sent in settling tank, more than sedimentation 72h;
(8) concentrated: concentrate the hydrolyzate after sedimentation according to a conventional method, concentrated proportion is 1.05-1.10;
(9) secondary deactivation: the temperature of deactivation is also 100 DEG C;
(10) secondary settlement: adopt settling tank to carry out secondary settlement, sedimentation 24h;
(11) secondary separation: adopt high speed channel separator to carry out secondary separation, rotating speed is that 15000-16300 turns/min, obtains protein hydrolysate concentrate;
(12) post processing: by directly canned for the protein hydrolysate concentrate obtained after secondary separation, obtain protein hydrolysate oral liquid, or spray-dried, obtain protein hydrolysate powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510720776.5A CN105341315A (en) | 2015-10-30 | 2015-10-30 | Protein hydrolyzate preparation technology |
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| CN201510720776.5A CN105341315A (en) | 2015-10-30 | 2015-10-30 | Protein hydrolyzate preparation technology |
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| CN105341315A true CN105341315A (en) | 2016-02-24 |
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| CN201510720776.5A Pending CN105341315A (en) | 2015-10-30 | 2015-10-30 | Protein hydrolyzate preparation technology |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107721729A (en) * | 2017-10-09 | 2018-02-23 | 湖南康哲农牧业发展有限公司 | A kind of organic fertilizer manufacture craft |
| CN109734773A (en) * | 2019-03-06 | 2019-05-10 | 康哲(湖南)制药有限公司 | A kind of preparation method of protein hydrolysate concentrate |
| CN109734774A (en) * | 2019-03-06 | 2019-05-10 | 康哲(湖南)制药有限公司 | A kind of preparation method of protein hydrolysate oral solution |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1757301A (en) * | 2005-09-19 | 2006-04-12 | 江南大学 | Method for preparing soybean protein by using heat treatment of soybean dregs |
| CN101264316A (en) * | 2008-05-08 | 2008-09-17 | 广西广明药业有限公司 | Preparation of medicinal protolysate |
| CN101766254A (en) * | 2009-12-31 | 2010-07-07 | 山东万得福实业集团有限公司 | Method for preparing low-sodium healthcare isolated soy protein |
| CN102763761A (en) * | 2012-06-21 | 2012-11-07 | 谷神生物科技集团有限公司 | Method for preparing enzymolysis low-sodium soybean isolated protein |
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2015
- 2015-10-30 CN CN201510720776.5A patent/CN105341315A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1757301A (en) * | 2005-09-19 | 2006-04-12 | 江南大学 | Method for preparing soybean protein by using heat treatment of soybean dregs |
| CN101264316A (en) * | 2008-05-08 | 2008-09-17 | 广西广明药业有限公司 | Preparation of medicinal protolysate |
| CN101766254A (en) * | 2009-12-31 | 2010-07-07 | 山东万得福实业集团有限公司 | Method for preparing low-sodium healthcare isolated soy protein |
| CN102763761A (en) * | 2012-06-21 | 2012-11-07 | 谷神生物科技集团有限公司 | Method for preparing enzymolysis low-sodium soybean isolated protein |
Non-Patent Citations (3)
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| 唐有根: "《农副化工产品生产技术》", 31 July 1998, 中国工业大学出版社 * |
| 张慜 等: "《水产类调理食品加工过程品质调控理论与实践》", 31 January 2013, 中国医药科技出版社 * |
| 顾学裘: "《药物制剂注解》", 31 May 1965, 人民卫生出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107721729A (en) * | 2017-10-09 | 2018-02-23 | 湖南康哲农牧业发展有限公司 | A kind of organic fertilizer manufacture craft |
| CN109734773A (en) * | 2019-03-06 | 2019-05-10 | 康哲(湖南)制药有限公司 | A kind of preparation method of protein hydrolysate concentrate |
| CN109734774A (en) * | 2019-03-06 | 2019-05-10 | 康哲(湖南)制药有限公司 | A kind of preparation method of protein hydrolysate oral solution |
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Application publication date: 20160224 |
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