CN109574887A - A method of extracting natural taurine from abalone internal organ - Google Patents
A method of extracting natural taurine from abalone internal organ Download PDFInfo
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- CN109574887A CN109574887A CN201811630414.7A CN201811630414A CN109574887A CN 109574887 A CN109574887 A CN 109574887A CN 201811630414 A CN201811630414 A CN 201811630414A CN 109574887 A CN109574887 A CN 109574887A
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- internal organ
- taurine
- abalone internal
- abalone
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C303/00—Preparation of esters or amides of sulfuric acids; Preparation of sulfonic acids or of their esters, halides, anhydrides or amides
- C07C303/42—Separation; Purification; Stabilisation; Use of additives
- C07C303/44—Separation; Purification
Abstract
The method that the invention discloses a kind of to extract natural taurine from abalone internal organ; it is related to marine product taurine extractive technique field; pass through the abalone internal organ natural thaw under freezing; then cleaned with clear water; and it is cut into narrow strip; and it places and is homogenized in tissue homogenate bruisher; the surface area that internal organ are contacted with water can be increased; to which the concentration of soup can be improved; increase the final recovery rate of taurine; purified water is added by degreasing container and carries out degreasing, can effective and quick degreasing, increase the purity of taurine after extracting.The prominent feature of the present invention is to carry out the extraction of taurine for raw material by cheap abalone internal organ; simple process, efficiently; equipment cost is low; the pollution of environment on the one hand can be reduced simultaneously for the recycling of abalone internal organ; on the other hand it is conducive to realize the comprehensive utilization of marine product resource, the added value of marine product also can be improved.
Description
Technical field
The present invention relates to marine product taurine extractive technique fields, more particularly to one kind, and natural ox is extracted from abalone internal organ
The method of sulfonic acid.
Background technique
Taurine is a kind of nonprotein sulfur amino acid, and the entitled 2-aminoethanesulfonic acid of chemistry, is necessary to human body
Nutrient.Research shows that taurine has the ability for increasing cellular anti-oxidant, free radical resisting damage and antiviral infringement.It is good
Good liver-protecting preparation has the function of enhancing eyesight, promotes brain development, relieving fatigue, while also having certain antitumor work
Property.Taurine is made an addition in baby milk powder and functional food extensively.With continuous improvement of people's living standards and right
The market potential of the gradually understanding of taurine function, the more secure natural taurine of safety is huge, and development prospect is good.With
Being constantly progressive for aquaculture technology, abalone annual output reached tens of thousands of tons, as the increase year by year of abalone yield and abalone add
The higher value application of industrial expansion, processing byproduct is particularly important.
In abalone process, since internal organ contain toxin, abalone toxin is that separation and Extraction has from abalone internal organ
The compound of skin light toxic action is the decomposition product of seaweed phyllins.This toxin is generally easy to assemble in spring
In the internal organ of abalone, there is photochemical activity.When human body has eaten the abalone containing this compound, and it is exposed to sunlight
In, which can promote the generation of the amine compounds such as the intracorporal histidine of people, tyrosine and serine, so as to cause the inflammation of skin
Disease and toxic reaction.Some researches show that, under the conditions of solar irradiation, the lethal dose of Mouse oral abalone toxin is 50 μ g/kg,
Humans and animals eat a certain amount of abalone internal organ and can also cause a disease, and need to be protected from light for a long time can mitigate the state of an illness.Thus account for its weight 20% ~
30% internal organ are often dropped, the internal organ of abalone, generally referred to as portion of the abalone body in addition to shell, head, foot, outer embrane
Point, account for about the 20%-30% of abalone weight, this not only results in waste of resources, and also brings serious problem of environmental pollution.Study table
It is bright, taurine is rich in abalone internal organ, and the method for extracting taurine in abalone internal organ domestic at present is very few.
The country mostly uses ion-exchange to prepare natural taurine greatly.This technique substantially process are as follows: pretreatment → water extraction
Take → removing protein (adjusting the measures such as soda acid, addition sedimentation agent, UF membrane) → ionic energy transfer → concentration → crystallization → recrystallization.
As granted patent (patent No.: ZL201010174544.1) disclose it is a kind of by aquatic product leftovers by ultrafiltration removal of impurities, strong acid
Property ion exchange, reverse osmosis concentration, decoloration, crystallization and etc. obtain high-purity natural taurine preparation method；Granted patent
(patent No.: ZL201210199181.6) discloses a kind of by homogenate, ultrasonic disruption, extraction, sulfosalicylic acid and ultrafiltration
Film secondary separation, strong acidic ion exchange purifying, concentration, crystallizing and drying and etc. from mussel extract taurine method.This
Class technique is cumbersome and needs large-scale equipment, and higher cost is cleaned using ion exchange, complicated for operation, and ion exchange
Resin regeneration needs a large amount of bronsted lowry acids and bases bronsted lowry, big to the pollution of environment.
Summary of the invention
The purpose of the present invention is to solve above-mentioned problems, and the one kind proposed extracts natural ox sulphur from abalone internal organ
The method of acid.To achieve the goals above, present invention employs following technical solutions:
A method of extracting natural taurine from abalone internal organ, this method comprises the following steps:
S1, by the abalone internal organ natural thaw under -17 DEG C of freezings, then cleaned with clear water, and be cut into the fillet of 1-3cm
Shape, and place and be homogenized in tissue homogenate bruisher；
S2, by the S1 it is obtained homogenate be added certain mass ratio water, and 100 DEG C at a temperature of boil 50-70min,
And slag is filtered, collect soup；
S3, the ethyl alcohol that certain volume score is added into soup, are placed in hot bath and extract certain number at a certain temperature,
It is cooling after extracting 60-150min minutes, obtain coolant liquid；
S4, the coolant liquid prepared in the S3 is moved into degreasing container, and the purified water that 5-10 times of coolant liquid weight is measured is added,
Agitating device stirring is opened, mixing speed 60-120r/min persistently stirs 20-60 minutes after being warming up to 75-90 DEG C, then
Heat preservation, which stands 2-4 hours, is layered grease；
S5, the S4 is prepared into resulting lower aqueous layer releasing, and is filtered through filter paper, wherein filtrate moves into inspissator, in temperature
It 65-85 DEG C, is concentrated under reduced pressure under the conditions of vacuum degree -0.04 to -0.08MPa, until when its specific gravity is 1.15~1.45, after drying both
Obtain taurine extract.
It is of the invention it is preferred in, the ratio of the homogenate and water that prepare in the S1 is 1:1,1:5,1:10,1:15,1:20
One of.
It is of the invention it is preferred in, the volume fraction of ethyl alcohol is one of 55%, 65%, 75%, 85%, 95% in the S3.
It is of the invention it is preferred in, the number extracted in the S3 is one of 1 time, 2 times, 3 times, 4 times, 5 times.
It is of the invention it is preferred in, the temperature extracted in the S3 is one in 50 DEG C, 60 DEG C, 70 DEG C, 80 DEG C, 90 DEG C
Kind.
The beneficial effects of the present invention are: by the abalone internal organ natural thaw under freezing, then cleaned with clear water, and
It is cut into narrow strip, and places and is homogenized in tissue homogenate bruisher, it is possible to increase the surface area that internal organ are contacted with water, to can be improved
The concentration of soup increases the final recovery rate of taurine.Purified water is added by degreasing container and carries out degreasing, it can be effectively and quick
Degreasing, increase extract after taurine purity.It is raw material progress that the prominent feature of the present invention, which is by cheap abalone internal organ,
The extraction of taurine, simple process, efficiently, equipment cost is low, simultaneously for abalone internal organ recycling on the one hand can subtract
On the other hand the pollution of few environment is conducive to realize the comprehensive utilization of marine product resource, the added value of marine product also can be improved.
Specific embodiment
The technical scheme in the embodiments of the invention will be clearly and completely described below, it is clear that described implementation
Example is only a part of the embodiment of the present invention, instead of all the embodiments.
Embodiment one
A method of extracting natural taurine from abalone internal organ, this method comprises the following steps:
S1, by the abalone internal organ natural thaw under -17 DEG C of freezings, then cleaned with clear water, and be cut into the narrow strip of 1cm,
And it places and is homogenized in tissue homogenate bruisher；
S2, the water that homogenate obtained in S1 is added to certain mass ratio, and 100 DEG C at a temperature of boil 50min, and filter
Slag collects soup；
S3, the ethyl alcohol that certain volume score is added into soup, are placed in hot bath and extract certain number at a certain temperature,
It is cooling after extracting 60min minutes, obtain coolant liquid；
S4, the coolant liquid prepared in S3 is moved into degreasing container, and the purified water of 5 times of coolant liquid weight amounts is added, unlatching is stirred
Device stirring is mixed, mixing speed 60-120r/min is warming up to after 75 DEG C and persistently stirs 20 minutes, and then heat preservation stands 2 hours
It is layered grease；
S5, the lower aqueous layer for being prepared into S4 are released, and are filtered through filter paper, and wherein filtrate moves into inspissator, at 65 DEG C of temperature, very
It is concentrated under reduced pressure under the conditions of reciprocal of duty cycle -0.04MPa, until both obtaining taurine extract after drying when its specific gravity is 1.15-1.45.
Embodiment two
A method of extracting natural taurine from abalone internal organ, this method comprises the following steps:
S1, by the abalone internal organ natural thaw under -17 DEG C of freezings, then cleaned with clear water, and be cut into the narrow strip of 2cm,
And it places and is homogenized in tissue homogenate bruisher；
S2, the water that homogenate obtained in S1 is added to certain mass ratio, and 100 DEG C at a temperature of boil 60min, and filter
Slag collects soup；
S3, the ethyl alcohol that certain volume score is added into soup, are placed in hot bath and extract certain number at a certain temperature,
It is cooling after extracting 100min minutes, obtain coolant liquid；
S4, the coolant liquid prepared in S3 is moved into degreasing container, and the purified water of 8 times of coolant liquid weight amounts is added, unlatching is stirred
Device stirring is mixed, mixing speed 60-120r/min is warming up to after 85 DEG C and persistently stirs 40 minutes, and then heat preservation stands 3 hours
It is layered grease；
S5, the lower aqueous layer for being prepared into S4 are released, and are filtered through filter paper, and wherein filtrate moves into inspissator, at 75 DEG C of temperature, very
It is concentrated under reduced pressure under the conditions of reciprocal of duty cycle -0.06MPa, until both obtaining taurine extract after drying when its specific gravity is 1.15-1.45.
Embodiment three
A method of extracting natural taurine from abalone internal organ, which is characterized in that this method comprises the following steps:
S1, by the abalone internal organ natural thaw under -17 DEG C of freezings, then cleaned with clear water, and be cut into the narrow strip of 3cm,
And it places and is homogenized in tissue homogenate bruisher；
S2, the water that homogenate obtained in S1 is added to certain mass ratio, and 100 DEG C at a temperature of boil 70min, and filter
Slag collects soup；
S3, the ethyl alcohol that certain volume score is added into soup, are placed in hot bath and extract certain number at a certain temperature,
It is cooling after extracting 150min minutes, obtain coolant liquid；
S4, the coolant liquid prepared in S3 is moved into degreasing container, and the purified water of 10 times of coolant liquid weight amounts is added, unlatching is stirred
Device stirring is mixed, mixing speed 60-120r/min is warming up to after 90 DEG C and persistently stirs 60 minutes, and then heat preservation stands 4 hours
It is layered grease；
S5, the lower aqueous layer for being prepared into S4 are released, and are filtered through filter paper, and wherein filtrate moves into inspissator, at 85 DEG C of temperature, very
It is concentrated under reduced pressure under the conditions of reciprocal of duty cycle -0.08MPa, until both obtaining taurine extract after drying when its specific gravity is 1.15~1.45.
The taurine recovery rate of 1. different feed liquid ratio of table
In a preferred embodiment of the invention, by by with the liquid ratio of water according to 1:1,1:5,1:10,1:15,1:20 successively
Tetra- groups of check experiments of A1, A2, A3, A4, A5 are set, as a result as table 1 shows.
The taurine recovery rate of the volume fraction of the different ethyl alcohol of table 2.
In a preferred embodiment of the invention, it is successively set by the volume fraction of different ethyl alcohol 55%, 65%, 75%, 85%, 95%
Five groups of check experiments of B1, B2, B3, B4, B5 are set, as a result as table 2 shows.
The taurine recovery rate of the different extracting times of table 3.
In a preferred embodiment of the invention, set gradually by different extracting times 1 time, 2 times, 3 times, 4 times, 5 times C1,
Five groups of check experiments of C2, C3, C4, C5, as a result as table 3 shows.
The taurine recovery rate of the different extraction temperatures of table 4.
In a preferred embodiment of the invention, it is set gradually by 50 DEG C of different extraction temperatures, 60 DEG C, 70 DEG C, 80 DEG C, 90 DEG C
Five groups of check experiments of D1, D2, D3, D4, D5, as a result as table 4 shows.
More than, it is merely preferred embodiments of the present invention, but scope of protection of the present invention is not limited thereto, it is any
Those familiar with the art in the technical scope disclosed by the present invention, according to the technique and scheme of the present invention and its invents
Design is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (5)
1. a kind of method for extracting natural taurine from abalone internal organ, which is characterized in that this method comprises the following steps:
S1, by the abalone internal organ natural thaw under -17 DEG C of freezings, then cleaned with clear water, and be cut into the fillet of 1-3cm
Shape, and place and be homogenized in tissue homogenate bruisher；
S2, by the S1 it is obtained homogenate be added certain mass ratio water, and 100 DEG C at a temperature of boil 50-70min,
And slag is filtered, collect soup；
S3, the ethyl alcohol that certain volume score is added into soup, are placed in hot bath and extract certain number at a certain temperature,
It is cooling after extracting 60-150min minutes, obtain coolant liquid；
S4, the coolant liquid prepared in the S3 is moved into degreasing container, and the purified water that 5-10 times of coolant liquid weight is measured is added,
Agitating device stirring is opened, mixing speed 60-120r/min persistently stirs 20-60 minutes after being warming up to 75-90 DEG C, then
Heat preservation, which stands 2-4 hours, is layered grease；
S5, the S4 is prepared into resulting lower aqueous layer releasing, and is filtered through filter paper, wherein filtrate moves into inspissator, in temperature
It 65-85 DEG C, is concentrated under reduced pressure under the conditions of vacuum degree -0.04 to -0.08MPa, until when its specific gravity is 1.15~1.45, after drying both
Obtain taurine extract.
2. a kind of method for extracting natural taurine from abalone internal organ according to claim 1, which is characterized in that described
The ratio of the homogenate and water that prepare in S1 is one of 1:1,1:5,1:10,1:15,1:20.
3. a kind of method for extracting natural taurine from abalone internal organ according to claim 1, which is characterized in that described
The volume fraction of ethyl alcohol is one of 55%, 65%, 75%, 85%, 95% in S3.
4. a kind of method for extracting natural taurine from abalone internal organ according to claim 1, which is characterized in that described
The number extracted in S3 is one of 1 time, 2 times, 3 times, 4 times, 5 times.
5. a kind of method for extracting natural taurine from abalone internal organ according to claim 1, which is characterized in that described
The temperature extracted in S3 is one of 50 DEG C, 60 DEG C, 70 DEG C, 80 DEG C, 90 DEG C.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110881563A (en) * | 2019-12-17 | 2020-03-17 | 大连工业大学 | Method for ultrasonic-assisted extraction of abalone visceral protein |
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