JPS6058074A - バチルス・ブレビスを形質転換する方法 - Google Patents
バチルス・ブレビスを形質転換する方法Info
- Publication number
- JPS6058074A JPS6058074A JP58165066A JP16506683A JPS6058074A JP S6058074 A JPS6058074 A JP S6058074A JP 58165066 A JP58165066 A JP 58165066A JP 16506683 A JP16506683 A JP 16506683A JP S6058074 A JPS6058074 A JP S6058074A
- Authority
- JP
- Japan
- Prior art keywords
- plasmid
- bacillus
- bacillus brevis
- plevis
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000013612 plasmid Substances 0.000 title claims abstract description 64
- 241000193764 Brevibacillus brevis Species 0.000 title abstract description 5
- 230000009466 transformation Effects 0.000 title description 2
- 230000001131 transforming effect Effects 0.000 claims abstract description 6
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 30
- 238000000034 method Methods 0.000 claims description 9
- 108090000623 proteins and genes Proteins 0.000 abstract description 18
- 102000004169 proteins and genes Human genes 0.000 abstract description 8
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 4
- 229960003276 erythromycin Drugs 0.000 abstract description 3
- 108091005804 Peptidases Proteins 0.000 abstract description 2
- 239000004365 Protease Substances 0.000 abstract description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 abstract description 2
- 239000012634 fragment Substances 0.000 abstract description 2
- 239000013605 shuttle vector Substances 0.000 abstract description 2
- 101000702488 Rattus norvegicus High affinity cationic amino acid transporter 1 Proteins 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 17
- 108090000637 alpha-Amylases Proteins 0.000 description 16
- 108020004414 DNA Proteins 0.000 description 12
- 108091008146 restriction endonucleases Proteins 0.000 description 10
- 102000004139 alpha-Amylases Human genes 0.000 description 8
- 238000003776 cleavage reaction Methods 0.000 description 8
- 230000007017 scission Effects 0.000 description 8
- 229940024171 alpha-amylase Drugs 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 7
- 241000894006 Bacteria Species 0.000 description 6
- 229930193140 Neomycin Natural products 0.000 description 6
- 229960004927 neomycin Drugs 0.000 description 6
- 244000063299 Bacillus subtilis Species 0.000 description 5
- 235000014469 Bacillus subtilis Nutrition 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
- 238000005119 centrifugation Methods 0.000 description 4
- 102000018697 Membrane Proteins Human genes 0.000 description 3
- 108010052285 Membrane Proteins Proteins 0.000 description 3
- 239000002202 Polyethylene glycol Substances 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 229930027917 kanamycin Natural products 0.000 description 3
- 229960000318 kanamycin Drugs 0.000 description 3
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 3
- 229930182823 kanamycin A Natural products 0.000 description 3
- 239000013600 plasmid vector Substances 0.000 description 3
- 229920001223 polyethylene glycol Polymers 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 2
- 210000002421 cell wall Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 210000001938 protoplast Anatomy 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- DNRJTBAOUJJKDY-UHFFFAOYSA-N 2-Acetyl-3,5,5,6,8,8-hexamethyl-5,6,7,8- tetrahydronaphthalene Chemical compound CC(=O)C1=C(C)C=C2C(C)(C)C(C)CC(C)(C)C2=C1 DNRJTBAOUJJKDY-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 101100433727 Caenorhabditis elegans got-1.2 gene Proteins 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000193385 Geobacillus stearothermophilus Species 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108010014251 Muramidase Proteins 0.000 description 1
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 102100037681 Protein FEV Human genes 0.000 description 1
- 101710198166 Protein FEV Proteins 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 229910052792 caesium Inorganic materials 0.000 description 1
- TVFDJXOCXUVLDH-UHFFFAOYSA-N caesium atom Chemical compound [Cs] TVFDJXOCXUVLDH-UHFFFAOYSA-N 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 239000013599 cloning vector Substances 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000000819 hypertonic solution Substances 0.000 description 1
- 229940021223 hypertonic solution Drugs 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 230000003362 replicative effect Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 229940035893 uracil Drugs 0.000 description 1
- 230000002747 voluntary effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58165066A JPS6058074A (ja) | 1983-09-09 | 1983-09-09 | バチルス・ブレビスを形質転換する方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58165066A JPS6058074A (ja) | 1983-09-09 | 1983-09-09 | バチルス・ブレビスを形質転換する方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6058074A true JPS6058074A (ja) | 1985-04-04 |
| JPH0156756B2 JPH0156756B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1989-12-01 |
Family
ID=15805214
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58165066A Granted JPS6058074A (ja) | 1983-09-09 | 1983-09-09 | バチルス・ブレビスを形質転換する方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6058074A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2589880A1 (fr) * | 1985-11-07 | 1987-05-15 | Shigezo Udaka | Procede pour l'expression de genes par bacillus brevis |
| EP0916733A1 (de) * | 1997-11-17 | 1999-05-19 | Roche Diagnostics GmbH | Verfahren und Kit zur Klonierung von Fragmenten |
| EP0919623A3 (en) * | 1997-11-17 | 1999-07-14 | Roche Diagnostics GmbH | Procedure and kit for cloning of DNA fragments |
| WO2005045005A1 (ja) | 2003-11-11 | 2005-05-19 | Higeta Shoyu Co., Ltd. | 新規ブレビバチルス・チョウシネンシス及び該微生物を宿主とするタンパク質の製造方法 |
| WO2006004067A1 (ja) * | 2004-07-06 | 2006-01-12 | Kaneka Corporation | ブレビバチルス属細菌を用いたプロテインａ様蛋白質の生産方法 |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5615300A (en) * | 1979-05-11 | 1981-02-14 | Gist Brocades Nv | Plasmid and its use |
| JPS57132895A (en) * | 1980-12-31 | 1982-08-17 | Parubua Irutsuka | Production of rearranged dna molecule and protein |
-
1983
- 1983-09-09 JP JP58165066A patent/JPS6058074A/ja active Granted
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5615300A (en) * | 1979-05-11 | 1981-02-14 | Gist Brocades Nv | Plasmid and its use |
| JPS57132895A (en) * | 1980-12-31 | 1982-08-17 | Parubua Irutsuka | Production of rearranged dna molecule and protein |
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2589880A1 (fr) * | 1985-11-07 | 1987-05-15 | Shigezo Udaka | Procede pour l'expression de genes par bacillus brevis |
| US4994380A (en) * | 1985-11-07 | 1991-02-19 | Shigezo Udaka | Process for expressing genes by Bacillus brevis |
| EP0916733A1 (de) * | 1997-11-17 | 1999-05-19 | Roche Diagnostics GmbH | Verfahren und Kit zur Klonierung von Fragmenten |
| EP0919623A3 (en) * | 1997-11-17 | 1999-07-14 | Roche Diagnostics GmbH | Procedure and kit for cloning of DNA fragments |
| WO2005045005A1 (ja) | 2003-11-11 | 2005-05-19 | Higeta Shoyu Co., Ltd. | 新規ブレビバチルス・チョウシネンシス及び該微生物を宿主とするタンパク質の製造方法 |
| US7655452B1 (en) | 2003-11-11 | 2010-02-02 | Higeta Shoyu Co., Ltd. | Brevibacillus choshinensis and process for prodcuing protein wtih use of the microbe as host |
| JPWO2006004067A1 (ja) * | 2004-07-06 | 2008-04-24 | 株式会社カネカ | ブレビバチルス属細菌を用いたプロテインａ様蛋白質の生産方法 |
| WO2006004067A1 (ja) * | 2004-07-06 | 2006-01-12 | Kaneka Corporation | ブレビバチルス属細菌を用いたプロテインａ様蛋白質の生産方法 |
| JP2010246569A (ja) * | 2004-07-06 | 2010-11-04 | Kaneka Corp | ブレビバチルス属細菌を用いたプロテインａ様蛋白質の生産方法 |
| EP2251425A1 (en) | 2004-07-06 | 2010-11-17 | Kaneka Corporation | Process for producing protein A-like protein with use of Brevibacillus genus bacterium |
| US8597908B2 (en) | 2004-07-06 | 2013-12-03 | Kaneka Corporation | Process for producing protein A-like protein with use of Brevibacillus genus bacterium |
| JP2014064589A (ja) * | 2004-07-06 | 2014-04-17 | Kaneka Corp | ブレビバチルス属細菌を用いたプロテインａ様蛋白質の生産方法 |
| JP2016063844A (ja) * | 2004-07-06 | 2016-04-28 | 株式会社カネカ | ブレビバチルス属細菌を用いたプロテインａ様蛋白質の生産方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0156756B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) | 1989-12-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| AU570709B2 (en) | Cloning and expression in industrial species | |
| AU620026B2 (en) | Molecular cloning and expression of genes encoding proteolytic enzymes | |
| AU620326B2 (en) | Stable gene amplification in chromosomal dna of prokaryotic microorganisms | |
| US5017477A (en) | Enhancing DNA sequencies derived from the sacQ gene | |
| JPS6058074A (ja) | バチルス・ブレビスを形質転換する方法 | |
| US6083718A (en) | Transformed industrial bacillus strains and methods for making and using them | |
| JPH04507045A (ja) | 誘導性発現ベクター | |
| Zaghloul et al. | High level of expression and stability of the cloned alkaline protease (aprA) gene in Bacillus subtilis | |
| EP0228726B1 (en) | Method for preparing proteins using transformed lactic acid bacteria | |
| JPS62122589A (ja) | Ｄｎａ塩基配列およびベクタ− | |
| JP2620852B2 (ja) | 新規プラスミド及びそれにより形質転換された新規微生物 | |
| JPH05284973A (ja) | 組換プラスミドおよびこれをベクターとして用いる異種蛋白質の分泌生産方法 | |
| JP2500312B2 (ja) | ヒト成長ホルモンの生産法 | |
| EP0186631B1 (en) | Psm112 plasmid vector for expression in bacillus subtilis | |
| JP2560214B2 (ja) | 分泌シグナルペプチドをコードするｄｎａ配列 | |
| JPS61166400A (ja) | 蛋白生産の方法 | |
| JPS60145090A (ja) | Ｄνａ | |
| JPH0817702B2 (ja) | 新規プラスミド及びその調製方法並びにそのプラスミドを含有する新規微生物 | |
| JPS61212288A (ja) | 蛋白製造法 | |
| JPS61282084A (ja) | 発現用ベクタ− | |
| JPH05252964A (ja) | 高発現ベクター、バチルス属細菌、およびペプチド又は蛋白質の製造方法 | |
| JPS63133986A (ja) | 分泌シグナルペプチドをコ−ドするｄｎａ配列 | |
| JPS60203194A (ja) | Ｄνａ | |
| JPH02227083A (ja) | 新規プラスミド | |
| JPS6349082A (ja) | マルトオリゴ糖生産に関与する遺伝子、対応する組み換えプラスミド及び組み換え微生物 |