JPS60260489A - Fertilization of fishery processed exhaust scum - Google Patents

Abstract

(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed Description of the Invention] <Industrial Application Field> This invention utilizes specific microorganisms to aerobically ferment scum from seafood processing effluent, which has conventionally been dumped without treatment technology. The present invention relates to a method for producing high quality organic fertilizer by.

In this specification, the above-mentioned "scum from fish processing wastewater" refers to primary dehydrated wastewater from the process of manufacturing processed fish products, processed fish, or residues containing fish internal organs, blood, etc. Fish scum refers to a sludge-like product obtained by partially separating water using a screen or flocculant, and then mechanically dewatering it using a dewatering machine such as a screw press or belt press, if necessary.

<Prior art> The scum from such seafood processing wastewater, that is, the fish scum, generally contains about 40 to 60% water (wt%, the same hereinafter) and further contains about 5 to 40% oil and fat. Because it also contains ingredients, drying is insufficient even if it is dried, and furthermore, if it is used as a fertilizer like fish meal, which is a high-quality organic fertilizer, it may cause damage to crops. It has become organic waste with no way of putting it to good use. Furthermore, when disposed of, problems arise such as high-protein and high-fat waste, which causes rapid deterioration and decomposition, and the generation of foul odor and pollution.

<Problems to be Solved by the Invention> Therefore, the present inventors aerobically fermented the above-mentioned fish scum, for which no processing technology had been available, to produce a fertilizer comparable to fish processing by-product fertilizers under the Fertilizer Control Law. With the aim of providing a method for producing high-quality organic fertilizer, we screened microorganisms suitable for such aerobic fermentation, and as a result, we discovered Mucor circinelloides, a type of fungus.
circinelloides) is nitrogen in the scum.

This invention was completed based on the discovery that it has the ability to effectively decompose fats and oils without reducing fertilizer components such as phosphoric acid.

<Means for solving and overcoming the problem> In other words, the method of converting scum from seafood processing wastewater into fertilizer according to this invention is to generate scum from fishery processing wastewater containing oil and fat using Mucor circinelloides! It is characterized by the following:

The present inventors separated the fat and oil content in the similar scum. A strain with excellent ability to - was isolated from fish scum of fish processing wastewater from the fish processing industry cooperative in Shiogama City, Yingcheng Prefecture, using potato dextrose agar (PDA) medium. This isolate was identified by the British identification agency ComIlonwealt.
h Mycol-logical ln5titut
Mucor circinelloides variety diegem, a Mucor genus variant known by e)
circinelloidesvar, Tieghc
v). According to the mycological properties of this strain, B1111. Tol 1ros11i+aaJoga
kuin Co11. , 16.109 (1966). In this specification, this strain is referred to as Muconob ricinelloides No. 3 strain.

Is the high resolution of oils and fats in the above-mentioned scums a property possessed only by the above-mentioned one mutant scale isolated by the present inventors, a property possessed by Mucor licinelloides, or a property common to all members of the genus Mucor? In order to investigate whether this is a characteristic, we investigated the fish skulls of various preserved strains of the genus Mucor shown in Table 1, as well as strains of fungi other than the genus Mucor. The oil and fat decomposition ability of was tested using the following method.

That is, slant 18 nutrients of various bacterial strains shown in Table 1 were each suspended in 5 cc of sterilized water to prepare a spore suspension. Next, each of the above spore suspensions was added to a petri dish containing about 20 (liters) of fish scum containing oil and fat (55% water content, 30 (including 1) oil and fat per 100 g of dried fish scum), and heated at 30°C. The amount of oil and fat decomposition was determined by measuring the amount of fat and oil decomposed in the chili scum that had been allowed to stand for three days.The results are shown in Table 1.

Table 1 Kazuyoshi 0. hffietomium ””IIlOmy
CeS)l Same as above (IFO3318): 12.
1 Same as above (No, 3 > 113,6 ml) Fragilis: □ As seen from Table 1, the ability to decompose fats and oils in scum varies greatly depending on the bacterial strain, and the same horsemeat can be decomposed in seconds or between different strains. There is a large difference in resolution depending on the species. In particular, the resolution differs depending on the species even for those belonging to the genus Mucor, and some of them do not grow in scum. However, those belonging to the genus 1 of Mucor narsinelloides are strains. It is noteworthy that all of them grow well in similar scum, and their ability to decompose fats and oils is significantly lower than that of other species in the genus Mucor or the genus Mucor. Mucor has a high fat and oil decomposition ability in similar scum.
This can be said to be a characteristic property common to the extreme strains of Sarcinelloides.

The general mycological properties of Mucor circinelloides used in this invention are as follows.

■ Morphology (PDA medium, 30℃, 7 days) Sporangium:
Smooth and ahophyse.

yellow Spore: open type Spore 9 stalk: long and has positive reachability.

■ Growth status (PDA medium, 30℃) At the beginning of growth, it is white. The color gradually changes from yellow to tan.

Aerial mycelium, two white to gray Substrate mycelium, two white to gray ■ Physiological properties Optimal growth ρF1 5-6 Optimal growth temperature: 30℃ Growth humidity 10-35℃ Moisture rate for growth: 20-100%.

When carrying out this invention, a spore suspension is prepared from Mucor circinelloides cultured in PDA medium with sterilized water, and this is sprinkled on fish scum and cultured at 30°C for 3 to 5 days to grow the bacteria. (Normally 1010 spores
fish scum (generally 40 to 60% water content and approximately 5 to 40% oil and fat content) is prepared in advance and used as the raw material to be processed.
The inoculum of the enemy is mixed in a range of about 5.about.10% with respect to the amount (containing), and the mixture is fermented under aerobic conditions at a temperature of about 20.degree. C. to 40.degree.

In aerobic fermentation, a mixture of the above-mentioned inoculum and raw fish scum is placed in a fermenter, and fermentation is carried out by stirring or by venting from the bottom of the fermenter. □Can be adopted. A particularly preferred fermentation treatment is to granulate the above mixture to form particles with a diameter of 3 to 7 m.
This is a method in which the pellets are formed into pellets of about 1.0 m in size, then charged into a fermentation tank and ventilated from the bottom of the tank. Since it is preformed into pellets, voids are formed between the pellets in the fermenter, providing effective aerobic fermentation conditions.

<Examples> The present invention will be further described below with reference to Examples and Test Examples.

Example 1 A spore suspension was prepared using sterilized water from Mucor zarcineroides (No. 3) that had been cultured on a PDΔ slant medium at 30° C. for 7 days. Autoclave sterilization <120℃, 10
The spore suspension was inoculated into the fish scum (55% moisture), which had been prepared (10 minutes), and cultured at 30°C for 5 days.
And so.

This inoculum 1 garlic fresh fish scum (moisture 57%) 9ko
Mix well and pour the mixture into a granulator directly. ! 5mm
After making pellets, they were placed in a cylindrical fermenter with ventilation from the bottom for aerated culture. The culture process at this time is not shown in the graph of FIG.

After 3 days of culture, the moisture content changed from 54% to 15.5%, the oil content changed from 31.0% to 18.5%, and IIH changed from 5.6 to 5.7. On the other hand, the obtained fermented fish soup h
The total nitrogen content of the fertilizer increased from 7.46% to 7.98%, and the product was comparable to the fertilizer produced in Fisheries Processing A1 under the Fertilizer Control Law.

Further, during the above culture period, the fermenter was kept open, but no bad odor was generated.

Example 2 Mucor sarcine L. ides (IFO67) was used as a bacterial strain.
Fish scum was fermented in the same manner as in Example 1 except that 46) was used. Table 2 shows the changes in water content, oil content, and total nitrogen over time.

Note to Table 2) The oil content and total nitrogen are % dry matter.As seen from Table 2, the moisture content increased from 55.2% to 24% after 3 days of incubation, and the oil content increased from 28% to 18.5%.
%, the total nitrogen content changed from 1% to 10.57% by 9°6. In addition, the fermented product was comparable to the fertilizer by-product of seafood processing under the Fertilizer Control Law.

Test Example 1゜Nikko Drunken Itsui (+Mechanical 11.
For the 2-day fermented fish scum and the 3-day fermented light-scum, changes in microbial activity in the soil when these were added to the soil were measured using a microbial system as described below.

First, add 20g of dry soil to 10ml of water, 50% of the maximum drainage capacity.
(equivalent to 105°C for 2 hours) and pretreated at 25°C for 2411i to prepare multiple soil samples, and add 100 + 110 of the above three types of fermented fish scum dried products (dried at 105°C for 2 hours) to each soil sample. and put it in a microbial calorimeter (Nippon Tuka Kikai Seisakusho 1' MC-6P).
The cells were cultured at 25°C for a predetermined period of time. This microbial calorimeter outputs the temperature rise due to microbial activity in the soil sample (μV
).

For comparison, tests were conducted in G) when dried fish scum that was not subjected to fermentation treatment was added to the soil sample, and when nothing was added to the soil sample.

The results are shown in the graph of FIG. Each curve in the graph represents the following:

A: When nothing is added to the soil sample B: When unfermented fish scum is added to the soil sample C: When fish scum that has been fermented for 1 day is added to the soil sample D: When fish scum that has been fermented for 2 days is added to the soil sample E: When adding 3-day fermented fish ska 11 to the soil sample As can be seen from this graph, the fermentation process] 111 L/
In contrast, the fish scum that had been fermented for 1 to 3 days according to the present invention was not easily decomposed in the soil and did not significantly activate microbial activity113). It shows that microbial degradability is good (curves C, D, and E).

Test Example 2 A Komatsuna germination test was conducted on fish scum fermented by this sealing and untreated fish scum.

Air-dried soil 3009 was mixed with 3 g of calcium carbonate and a specified amount of fish snoum (total nitrogen content of 150 mg/100 g of air-dried soil) and packed in a 1:20,000 Earl Neubeyer pot, which was 60% of the maximum water volume. The water content was adjusted so that The fish cams used were one that had been fermented for four days in the same manner as in the previous example, and one that had not been fermented.

Table 3 shows the results of sowing 20 late-season Komatsuna seeds per pot in the above pots and measuring the germination i of each pot.

For comparison, the results of similarly measuring the germination rate when commercially available fish meal was used instead of similar scum are also shown.

As can be seen from Table 3, the germination failure observed when using untreated fish scum is effectively alleviated with the fermented scum of the present invention, resulting in a germination rate equal to or higher than that of fish meal. Indicated.

Table 3 <Effects of the Invention> As explained above, according to the present invention, by fermenting scum using Mucor sarcinelloides, which has an extremely excellent ability to decompose oil and fat in scum, The oil and fat content can be effectively reduced in a relatively short period of about 2 to 3 days without reducing the fertilizer components, and it is possible to produce a fertilizer comparable to fertilizers produced by fish processing by-products under the Fertilizer Control Law.

The fertilizer obtained in this way is decomposed in the soil, activates the activity of microorganisms in the soil, and also shows the same effect on improving germination rate as when fertilizing with fish meal, compared to other fertilizers that are not fermented. No germination problems such as scum were observed.

Therefore, the present invention is a useful method for making effective use of scum from fish processing wastewater, which has hitherto been unusable due to its poultry fat content, and turning it into high-quality organic fertilizer.

[Brief explanation of drawings]

FIG. 1 is a graph showing the progress of culture when the present invention is carried out, and shows changes in oil and fat content, total nitrogen content, and water content in the scum and changes in fermentation temperature over time. FIG. 2 is a graph showing the results of measuring changes in microbial activity in soil using a microbial calorimeter when scum fermented according to the present invention was added to soil.

Claims (1)

[Claims] 1. A method for converting fishery processing wastewater scum into fertilizer, which comprises subjecting the scum from fishery processing wastewater containing oil and fat to fermentation using Mucor circinelloides.