JP2003095773A - Fertilizer utilizing waste molasses and method of producing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a fertilizer which effectively utilizes waste molasses as a fertilizer by fermenting the waste molasses and utilizes the waste molasses to be fermented without causing offensive odors by soil fungi, and to provide a method of producing the fertilizer. SOLUTION: An inoculum medium is produced by diluting the waste molasses with water, mixing a leaf mold collected from the roots of Acacia confusa Merr therewith and fermenting the mixture. This inoculum medium is then mixed with the waste molasses and the mixture is fermented. Putrefactive bacteria do not proliferate with predominance characteristic and lactic acid bacteria and yeast bacteria proliferate and therefore the good-quality fertilizer containing much organic matter is obtained without producing the offensive odors.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to a fertilizer using molasses and a method for producing the fertilizer.

[0002]

2. Description of the Related Art In the sugar industry, syrup-like molasses is by-produced as a mother liquor remaining after crystallization of sugar from a concentrated juice of sugar beet or sugar beet. The molasses, which is a by-product of the sugar industry, has a high sugar content of 70 to 80% and is partially used as livestock feed. Further, it is used as a raw material for the production of butanol, acetone, citric acid and ethyl alcohol, and is added to the fermentation residual liquid after extraction of citric acid and ethyl alcohol with other fertilizer elements before being sold as fermented liquid fertilizer. Further, the molasses obtained after the above molasses is further recovered by applying a chromatograph to the molasses has a low sugar content of 16 to 18%, and most of the molasses is currently discarded into the ocean without being reused.

[0003]

In recent years, this ocean dumping has been prohibited, and it has become difficult to treat the molasses that has been chromatographed. In addition, some of the molasses that have been chromatographed are processed at compost factories, but this molasses is difficult to process due to its high water content, and the amount of processing is limited. ing. The present invention was made in order to solve such conventional problems, and the purpose thereof is to effectively utilize as a fertilizer by fermenting molasses that has been conventionally discarded, and to produce offensive odor by soil bacteria. It is intended to provide a fertilizer using waste molasses that is fermented without being generated and a method for producing the fertilizer.

[0004]

[Means for Solving the Problems] As a means for achieving the above object, in the method for producing a fertilizer according to claim 1 of the present invention, waste molasses is mixed with humus and fermented by the action of soil fungus contained in the humus. To obtain a fermentation product liquid.

In the method for producing a fertilizer according to claim 2, the molasses is mixed with the molasses and fermented to produce an inoculum culture solution,
Then, the inoculum culture solution was mixed with the molasses and fermented to obtain a fermentation product solution.

In the method for producing a fertilizer according to claim 3, at least a part of the fermentation product liquid obtained by the method according to claim 1 or 2 is used as a seed bacterium, and the seed bacterium is mixed with the molasses and fermented. The fermentation product solution was obtained by sequentially using at least a part of the previous fermentation product solution as the inoculum for the next fermentation.

In the method for producing a fertilizer according to claim 4, the method for producing a fertilizer according to claim 1, 2 or 3 is a method in which diluted molasses diluted with water is used as molasses.

In the method for producing a fertilizer according to claim 5, in the method for producing a fertilizer according to claim 1, 2, 3 or 4, the mulch is mulch collected from the root of a fantasy tree.

The fertilizer according to claim 6 is a fertilizer using the molasses obtained by the method according to claims 1 to 5.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION As the molasses used in the present invention, there can be used ordinary molasses having a high sugar content and molasses having a low sugar content after being subjected to a chromatographic apparatus. Ordinary molasses is the remaining syrup-like mother liquor obtained by recovering sugar from sugar cane or sugar beet sugar solutions in the sugar industry and has a sugar content of 70% to 80%. Table 1 shows an example of the analysis results of this molasses.

[Table 1] The components of the fertilizer obtained by fermenting the stock solution shown in Table 1 with soil fungi are shown in Tables 2 and 3.

[Table 2]

[Table 3]

Further, the sugar content of the molasses after the usual molasses has been subjected to the chromatograph is reduced to 16 to 18%. Table 4 shows an example of the analysis results of this low-sugar waste molasses.

[Table 4] Note that these molasses are examples, and it is also possible to use molasses having a lower concentration. The mulch used in the present invention is a mixture of organic substances in the decomposition stage where plants are decomposed in the natural world, and forms a bacterial bed where various degrading bacteria live. The mulch soil formed by decomposing fallen leaves and the like forms a mixture of various organic substances and is a soil-like material containing a large amount of various decomposing bacterial groups and their beds. In the present invention, as long as it is about mulch, it can be used regardless of the place of production and the plant species that has formed mulch.

As described above, various kinds of mulch can be used in the present invention. Among various kinds of mulch, from the periphery of Soshiki (scientific name: Acacia confusa Merr) called "shousugi" in Okinawa prefecture. The collected mulch is suitable. This "Shousugi" is a leguminous plant native to Hishima, Taiwan, which grows naturally in the northern part of Okinawa Prefecture. At the base of this legume, root nodule bacteria inhabit and form a fertile soil.

In fermenting molasses in the present invention, mulch can be directly added to molasses. However, molasses, especially high molasses molasses obtained by crystallizing sugar, has a high sugar content and is not suitable for survival of microorganisms. Therefore, it is advantageous to dilute the molasses with water to reduce the concentration of sugar and perform fermentation. If the sugar concentration is too high, the environment becomes unsuitable for the survival of microorganisms and the fermentation is inhibited as described above, and the treatment period becomes long, and in extreme cases, the treatment becomes impossible. On the other hand, if the sugar concentration is too low, nutrients will be insufficient and useful microorganisms will not function effectively, resulting in a longer treatment period, and the quality of the fertilizer obtained will deteriorate due to the effects of contaminants, especially spoilage bacteria. There is a risk of In the case of high molasses molasses, it is appropriate to dilute the sugar so that the sugar concentration is about 10 to 20% (dilution degree about 1: 3.5 to 8).

In the case of the low-sugar molasses subjected to chromatographic treatment, the sugar concentration is 16 to 18%, which is within the range of the sugar concentration when the above-mentioned high-sugar molasses is diluted. Fermentation could and should be possible. However, when the low-sugar molasses subjected to chromatographic treatment is used, it is diluted so that the sugar concentration is lower than that of the high-sugar molasses, which is about 3 to 7% (dilution degree of about 1 to 2 to 5). Is appropriate. If the sugar concentration is higher than the above range, the treatment period will be prolonged, and if the sugar concentration is lower than the above range, the treatment period will be prolonged, and the fertilizer quality may be deteriorated due to contaminants.

Either cold water or hot water can be used as the diluting water. However, if the water temperature is too low, the solubility of the molasses is reduced and the dissolution rate is reduced, so that the time required for the dilution is prolonged and the resulting dilution is obtained. The liquid temperature of the liquid becomes low, and the period of fermentation treatment is prolonged. Further, even if the temperature of the diluting water is too high, the temperature of the diluting liquid becomes a high temperature unsuitable for the survival of useful microorganisms, so that the treatment period is prolonged. Therefore, it is preferable to use warm water that is slightly heated so that the liquid temperature after dilution is 20 to 30 ° C at which useful microorganisms can effectively function. Completion of fermentation can be detected by forming a layer of yeast on the surface of the fermentation broth.

[0016] In the present invention, as described above, it is possible to directly add mulch to the molasses or its diluted solution to ferment it. However, an inoculum culture solution is prepared in advance, and this inoculum culture solution is used. When fermentation is carried out, stable fermentation can be carried out without requiring a long period of time for fermentation. Similarly to the fermentation for the fertilizer production, the molasses culture uses the molasses or its diluted solution as a medium, and mulch is added thereto to grow useful microorganisms. Further, the liquid fertilizer itself obtained by the method of the present invention can be used as the seed bacterium, and it is more preferable. That is, the use of at least a part of the fermentation product liquid of the previous fermentation as an inoculum for the next fermentation is repeated. In this case, it is desirable to use fresh one just after production. The culture conditions of the inoculum culture are the same as the above fermentation method in which mulch is added to molasses to directly produce a fertilizer. That is, the sugar concentration is about 10 to 20% in the case of normal high-sugar molasses,
For low-sugar waste molasses, it is about 3-7%. The present inventor has found that the growth of harmful microorganisms such as spoilage bacteria is suppressed and useful microorganisms can selectively grow within this range. The culture temperature is the same as the fermentation process for fertilizer production 20
~ 30 ° C. Completion of the culture can be detected by forming a yeast layer on the surface of the culture solution as in the fermentation process.

The conditions of the fermentation process in the fermentation process for producing a fertilizer using the inoculum culture solution are the same as the conditions of the fermentation process of directly adding the mulch. As a result of research on the above-mentioned inoculum culture solution and useful microorganisms in fermentation for fertilizer production, the present inventors have found that it is a complex microorganism group in which yeast and lactic acid bacteria coexist as described later.

[0018]

[Examples] Hereinafter, a method for producing a fertilizer using molasses will be described. Example 1 In this example, a fertilizer using normal molasses with a high sugar content is manufactured by the following procedure. (1) First, 20 liters of normal molasses having the composition of the above Table 1 is placed in a tank, and 20 liters of water are mixed and stirred to dissolve the molasses in water. Here, cold water at room temperature or warm water having a temperature after mixing which is 20 to 30 ° C. of the enzyme activation temperature is mixed. (2) In a tank in which this molasses and water were dissolved, add 3 leaves of mulch.
Add another 0 liter and mix. As this mulch soil, the soil collected from the area around Sosogi (Scientific name: Acacia confusa Merr) "Shousugi" is used. (3) After adding mulch, add 30 liters of water,
Stir to a total volume of 100 liters. As a whole, the mixing ratio of molasses, water and mulch is volume ratio (2: 5: 3)
However, it can be implemented even if there is some increase or decrease. (4) After that, the tank is capped and fermented for about 2 weeks by the action of soil bacteria (microorganisms contained in mulch). 10 to
Bubbles are generated in a lumpy state around the 14th day, and after a while, a layer of yeast is generated, and in this state, it can be sprayed as a liquid fertilizer to the field.

[Example 2] In this example, a fertilizer is produced by using the culture solution obtained in the above-mentioned Example 1 as an inoculum. (1) First, 14 liters of normal molasses having the composition shown in Table 1 was put in a tank as shown in FIG.
Mix and dilute 1 liter. Here, cold water at room temperature or warm water having a temperature after mixing which is 20 to 30 ° C. of the enzyme activation temperature is mixed. At this time, the mixing ratio of the molasses and water is the volume ratio (1: 5.92). If the mixing ratio of molasses and water is in the range of about (1: 3.5) to (1: 8), it can be applied efficiently, and even if it is outside the range, it can be carried out. (2) Next, 3 liters of the supernatant of the culture solution obtained in Example 1 is added, the tank is capped, and the fermentation is continued for about 2 weeks. (6) As the fermentation progresses, the surface of the yeast becomes 3 to 7 thick.
It is expressed in mm. In this state, it can be applied to the field as liquid fertilizer.

Example 3 (1) As shown in FIG. 1, 32 liters of chromatographically treated molasses having the composition shown in Table 4 above is placed in a tank and mixed with 65 liters of water for dilution. Here too, the cold water at room temperature or the temperature after mixing is 20 to 30 ° C. of the enzyme activation temperature.
Mix warm water to the extent that At this time, the mixing ratio of the molasses and water is about (1: 2) by volume. If the mixing ratio of molasses and water is in the range of about (1: 2) to (1: 5), it can be applied efficiently, and even if it is outside the range, it can be carried out. (2) Next, 3 liters of the supernatant of the culture broth obtained in Example 2 is added, the tank is capped and fermented for about 2 weeks. (3) As fermentation progresses, yeast appears on the surface with a thickness of 3 to 7 mm. In this state, it can be applied to the field as liquid fertilizer.

Table 5 shows the results of identification of bacteria predominantly separated from this sample using the liquid fertilizer produced by the above method as a sample.

[Table 5] -Test method After measuring the number of general bacteria (viable count) of the sample (standard agar medium, 35 ° C, culturing for 2 days), the colonies predominantly grown on the culture plate were picked and isolated. The isolated bacteria were identified by morphological observation and physiological property tests.・ Test result The isolated strain was identified as Lactobacillus Plantarum. The property test results are shown in Table 5. The general bacterium count (viable count) per 1 g of the sample was 1.5 × 10 ⁷ . Lactobac
illus (lactobacillus) is a gram-positive bacillus that ferment sugar to mainly produce lactic acid, and is isolated from foods such as lactic acid bacteria and grains, silage, and intestinal tract of animals.

Next, Table 6 shows the results of identification of yeasts that are predominantly separated from the specimen.

[Table 6] -Test method After measuring the number of yeasts of a sample (potato dextrose agar medium, culture | cultivating at 25 degreeC, 7 days), the colony which predominantly grew on the culture | cultivation plate was used as the isolated yeast by fishing. The isolated yeast was morphologically observed and tested for characteristics and identified with reference to literatures.・ Test results The isolated yeast was identified as Candida valida. The property test results are shown in Table 6. The number of yeasts per 1 g of the sample was 8.
It was 0 × 10 ³ . Candida is an ascomycetous incomplete yeast. Vegetative cells are mainly spherical, elliptical, cylindrical,
It becomes elongated, grows in multipolar sprouting, and does not form spores and spores. It is widely distributed in nature and is known to be isolated from general environments such as soil, air and water, plants, foods and animals including humans.・ Reference Kurtman.CPand Fell.JW: "The Yeasts, A Taxo
nomic Study "4th edition (1998) Elscvier Science B.
V. Barnett.JA, Payne, RWand Yarrow, D .: "Yeasts:
Characteristics and identification "3rd edition (2
000) Cambridge University Press.

As shown in these test results, it is shown that lactic acid bacteria and yeast are propagated in the fertilizer produced by the present invention. Further, since the lactic acid bacterium is fermentatively fermented, no offensive odor is generated, and long-term storage is possible. That is, since only microorganisms that can grow in a liquid having a high sugar content, such as molasses, can survive, the spoilage bacteria do not work with superiority, and the lactic acid bacteria and yeast bacteria propagate as described above.

Although the embodiment of the present invention has been described above, the specific configuration of the present invention is not limited to this embodiment, and there are design changes and the like within the scope not departing from the gist of the invention. However, it is included in the present invention. For example, Saccharomyces, Candida, Pichia, Zygo as an alternative to the leaf soil.
saccharomyces, Schizosaccharomyces, Hanseniaspor
It is also possible to produce a similar fertilizer by using a yeast of any one of a, Dekkera and Kluyveromyces and adding lactic acid bacteria to the yeast to ferment. Further, the mixing ratio of water and the mixing ratio of mulch soil is not limited to the range of the examples, and can be carried out within a certain range. Furthermore, although the soil collected from the root of the thought tree was used in the above-mentioned Examples, the present invention includes the case where other soil is used.

[0025]

EFFECTS OF THE INVENTION In the method for producing a fertilizer using molasses according to claims 1 to 5, fermentation is performed by the action of soil bacteria contained in mulch so that no special equipment is required and the cost is relatively low. Moreover, fertilizer can be easily manufactured. Further, if the inoculum culture solution is produced in advance, the fermentation can be efficiently performed with the culture solution having a high concentration, and a large amount of molasses can be treated in a relatively short period of time. Since spoilage bacteria do not proliferate with advantage and lactic acid bacteria and yeasts propagate,
A good quality fertilizer containing no organic odor and containing a large amount of organic matter can be obtained. Then, if the soil collected from the root of the thought tree containing a lot of effective microorganisms is used, the fermentation can be efficiently carried out.

Further, in the fertilizer according to claim 6, since the fertilizer is fermented by the action of soil fungus contained in mulch,
The molasses can be effectively used as a fertilizer. Also,
Since it decomposes sugar contained in molasses, good quality fertilizer containing a large amount of organic matter can be obtained.

[Brief description of drawings]

FIG. 1 is an explanatory view showing a method for producing fertilizer.

Claims (6)

[Claims] 1. A method for producing a fertilizer, which comprises mixing a molasses with molasses and fermenting it by the action of soil fungi contained in the molasses to obtain a fermentation product liquid. 2. A fertilizer characterized in that a molasses is mixed with molasses and fermented to produce an inoculum culture solution, and then the inoculum culture liquid is mixed with waste molasses and fermented to obtain a fermentation product liquid. Production method. 3. Use at least a part of the fermentation product liquid obtained by the method according to claim 1 or 2 as a seed bacterium, and mix the seed bacterium with molasses to ferment to obtain a fermentation product liquid. A method for producing a fertilizer, which comprises using at least a part of the fermentation product liquid of 1. 4. The method for producing a fertilizer according to claim 1, wherein diluted molasses diluted with water is used as molasses. 5. The leaf mold of claim 1, 2, 3 or 4 wherein the leaf mold is leaf mold collected from the root of a fantasy tree.
The method for producing the fertilizer described. 6. A fertilizer using molasses obtained by the method according to claim 1.