JPS60126087A - プロモ−タ・クロ−ニング用プラスミドｐＴＰ１８−２，ｐＴＰ１８−４及びｐＴＰ１８−６ - Google Patents
プロモ−タ・クロ−ニング用プラスミドｐＴＰ１８−２，ｐＴＰ１８−４及びｐＴＰ１８−６Info
- Publication number
- JPS60126087A JPS60126087A JP58234051A JP23405183A JPS60126087A JP S60126087 A JPS60126087 A JP S60126087A JP 58234051 A JP58234051 A JP 58234051A JP 23405183 A JP23405183 A JP 23405183A JP S60126087 A JPS60126087 A JP S60126087A
- Authority
- JP
- Japan
- Prior art keywords
- ptp18
- restriction enzyme
- plasmid
- site
- cloning
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000013612 plasmid Substances 0.000 title claims abstract description 28
- 238000010367 cloning Methods 0.000 title claims abstract description 19
- 108091008146 restriction endonucleases Proteins 0.000 claims abstract description 43
- 230000000694 effects Effects 0.000 claims abstract description 8
- 108090000204 Dipeptidase 1 Proteins 0.000 claims abstract description 5
- 238000013518 transcription Methods 0.000 claims abstract 2
- 230000035897 transcription Effects 0.000 claims abstract 2
- 238000003776 cleavage reaction Methods 0.000 claims description 30
- 230000007017 scission Effects 0.000 claims description 30
- 108010022394 Threonine synthase Proteins 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 6
- 230000002103 transcriptional effect Effects 0.000 claims description 6
- 241001646716 Escherichia coli K-12 Species 0.000 claims description 4
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 101150074155 DHFR gene Proteins 0.000 abstract description 2
- 238000006243 chemical reaction Methods 0.000 description 19
- 108020004414 DNA Proteins 0.000 description 15
- 239000000243 solution Substances 0.000 description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 239000012634 fragment Substances 0.000 description 5
- 102000012410 DNA Ligases Human genes 0.000 description 3
- 108010061982 DNA Ligases Proteins 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 102000004419 dihydrofolate reductase Human genes 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 229960000723 ampicillin Drugs 0.000 description 2
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 2
- 235000011089 carbon dioxide Nutrition 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000006916 nutrient agar Substances 0.000 description 2
- 150000002989 phenols Chemical class 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 101100148606 Caenorhabditis elegans pst-1 gene Proteins 0.000 description 1
- 101100257127 Caenorhabditis elegans sma-2 gene Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- HEAFLBOWLRRIHV-UHFFFAOYSA-N [Na].[P] Chemical compound [Na].[P] HEAFLBOWLRRIHV-UHFFFAOYSA-N 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 229960001931 ampicillin sodium Drugs 0.000 description 1
- KLOHDWPABZXLGI-YWUHCJSESA-M ampicillin sodium Chemical compound [Na+].C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C([O-])=O)(C)C)=CC=CC=C1 KLOHDWPABZXLGI-YWUHCJSESA-M 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- IEDVJHCEMCRBQM-UHFFFAOYSA-N trimethoprim Chemical compound COC1=C(OC)C(OC)=CC(CC=2C(=NC(N)=NC=2)N)=C1 IEDVJHCEMCRBQM-UHFFFAOYSA-N 0.000 description 1
- 229960001082 trimethoprim Drugs 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58234051A JPS60126087A (ja) | 1983-12-12 | 1983-12-12 | プロモ−タ・クロ−ニング用プラスミドｐＴＰ１８−２，ｐＴＰ１８−４及びｐＴＰ１８−６ |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP58234051A JPS60126087A (ja) | 1983-12-12 | 1983-12-12 | プロモ−タ・クロ−ニング用プラスミドｐＴＰ１８−２，ｐＴＰ１８−４及びｐＴＰ１８−６ |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS60126087A true JPS60126087A (ja) | 1985-07-05 |
| JPS6335236B2 JPS6335236B2 (enrdf_load_html_response) | 1988-07-14 |
Family
ID=16964798
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP58234051A Granted JPS60126087A (ja) | 1983-12-12 | 1983-12-12 | プロモ−タ・クロ−ニング用プラスミドｐＴＰ１８−２，ｐＴＰ１８−４及びｐＴＰ１８−６ |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS60126087A (enrdf_load_html_response) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6339586A (ja) * | 1986-05-02 | 1988-02-20 | ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ | バチルスの細胞外蛋白合成のための分泌シグナル選択ベクタ− |
-
1983
- 1983-12-12 JP JP58234051A patent/JPS60126087A/ja active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6339586A (ja) * | 1986-05-02 | 1988-02-20 | ギスト ブロカデス ナ−ムロ−ゼ フエンノ−トチヤツプ | バチルスの細胞外蛋白合成のための分泌シグナル選択ベクタ− |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6335236B2 (enrdf_load_html_response) | 1988-07-14 |
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