JPS5961751A - Scanning apparatus of densitometer - Google Patents

Abstract

PURPOSE:To simplify the constitution of the scanning apparatus of a densitometer, by using a light blocking rotary disc provided with a pervious aperture and an optical fiber bundle of which both end parts respectively have predetermined arrangements. CONSTITUTION:Light passing through the pervious aperture 15 of a light blocking disc 14 from a light source is successively incident to optical fibers 2, 3... forming an optical fiber bundle of which end parts 2b, 3b... are arranged on a circumference and successively emitted from the other ends 2a, 2b where the fibers 2, 3... are linearily arranged to successively and linearly scan and irradiate a thin chromatography plate P. Similar operation is performed in a light receiving side. By this mechanism, the constitution of the scanning apparatus of a densitometer becomes simple.

Description

DETAILED DESCRIPTION OF THE INVENTION This invention applies to the densitometer's scanning device Vt. The present invention relates to a scanning device for moving the minute portion in a densitometer that performs optical measurement of the minute portion and moves the minute portion so as to cover a desired area on a chromatographic support or the like.

A densitometer that measures a non-uniform concentration distribution such as a spot on a chromatographic support, etc. irradiates a minute light beam onto the chromatographic support, performs optical measurement of a minute portion, and measures a desired area on the chromatographic support, etc. A small beam of light is scanned in a zigzag or rectangular pattern to cover the area. For example, in the densitometer shown in Utility Model Application Publication No. 55-147655, the minute light flux is kept stationary, a chromatography support, etc. is placed on a movable stage, and the movable stage is moved in the X and Y directions. Rectangular scanning is performed.

However, the conventional scanning device as described above has a problem in that it has a complicated structure and the time required for scanning is relatively long.

This invention was made in view of this situation.
To provide a scanning device that has a simple configuration and can relatively shorten the time required for scanning.

Hereinafter, the present invention will be explained in detail with reference to the drawings.

However, this invention is not limited thereby.

(1) shown in the FI diagram is an embodiment of the scanning device of the densitometer of the present invention, in which a large number of optical fibers (2) to
It basically consists of a light-shielding disc (14) having a transparent hole θ pupil.

A large number of optical fibers (2) to one end of the θ cable (2a)
- (13a) are arranged in a straight line and face the W'JF4 chromato plate (P). Also, on the other hand, IT
T! ! The end portions (2b) to (13b) are arranged in a circular shape d and are opposed to the light shielding disc 0 so as to correspond to the locus of the through hole OQ formed when the light shielding disc 0→ is rotated. The optical fibers (2a) to (13a) each have a 111 diameter of 0.2 to 0.5 fl, and are arranged in a number that is longer than the width of the object to be measured.

The light-shielding disc (1→) is accurately rotated at a constant speed using a pulse motor or the like.The diameter of the through hole 0Q is approximately equal to the diameter of the optical fibers (2) to (13).

In this device α, a light source unit (not shown) is installed on the opposite side of the optical fiber (2) to α1.
11, the light aη becomes a minute flux through one optical fiber [(2) in Fig. 1] that corresponds to the transparent hole (II) when the light is transmitted through a, and the thin layer corresponding to the first part of the optical fiber Microscopic area CEi on chromato plate 1- (1”)
In Figure S1, it is irradiated to (2z).So, for example, for a thin J171 plate (P), an optical fiber (2) is irradiated.
If a light receiving section (not shown) is provided on the opposite side from the ~θ dye, optical measurement of the minute portion (w2 in FIG. 1) can be performed. When the light-shielding disc (14) is rotated, the optical fiber corresponding to the through hole U-uniform changes sequentially from (2) to (1-niy), so
Along with this, the minute part also moves from (2δ to (33)).In this way, '+'li I? J chromato plate (P) is scanned.

In the above explanation, if the light source part and the light receiving part are replaced, the light of a minute part will be detected by the light receiving part via any of the optical fibers (2) to (li) and the through hole (14G). Thin layer chromatograph (P) is the one in which optical fibers (2) to (11) are lined up according to the rotation of the light-shielding disc CIA.

Thin layer chromatography plate (P) is connected to optical fibers (2) ~ (
1:) at right angles to the direction in which they are lined up, the thin layer chromatography plate (P) becomes 2 as shown in Figure 2 (711).
Scanned dimensionally. The mechanism for moving the thin layer chromatography plate (P) only in one direction is significantly simpler than the conventional '+ shallow ('M) for moving it in the X and Y directions.

Not shown in the third factor (5→ is the scanning value Mffi 1 of this invention
41) is an example of a densitometer. Similar to the scanning device (1) shown in FIG. After reflecting at the In Ir'l chromato plate (P), it is irradiated onto the In Ir'l chromato plate (P).t-1rj (is a through hole).The light source section consists of a light source lamp (51λ light source mirror (52)), a slit ( 53), interference filter (5, Q
L/7 X' (55) and beam mixer (56)
It consists of The light becomes monochromatic light by an interference filter (54), and its intensity is made uniform by a beam mixer (56).

The thin layer chromatography plate (P) is equipped with a moving stage (57
), 1tti:1.13. (58) is a light receiver, which uses the light from the thin counterfeit V print plate 1- (P) and the (hyper-active stage (57)) as a core. (59) is a microcomputer, ), performs light measurement in synchronization with the rotation of the light-shielding disc (44), and controls (151) the child j+II of the moving stage (57), 1 and reconnaissance of the measurement data of each minute part, etc. The determination will be carried out in an analysis.

As a modified example, 115 layer chromatography plate (P)
There are those that measure weak light from the outside, those that rotate a light-shielding disc in steps, and those that perform two-wavelength densitometry by setting interference filters with different wavelengths on the rotor and rotating the rotor. is excited.

Furthermore, as shown in FIG. 4, an optical fiber (60) may be used instead of the beam mixer (56). In this case, the -blade end of the optical fiber (60) is opposed to the light-shielding disk (4) in the same way as the other end of the optical fiber (60), and the other end is closely bundled with the lens ( 55
) is faced. Energy can be used more effectively than the beam mixer (56).

Furthermore, as shown in FIG.
) and the light-receiving side optical fiber 11\(62), and the light-shielding disks on the irradiation side and the light-receiving side are rotated synchronously so that the corresponding optical fibers are selected at the same time.

Further, as shown in FIG. 6, a group of optical fibers (63) may be arranged in two rows to fill the same gap between the optical fibers as in one row.

As can be understood from the above description, the present invention has one end of optical fibers having a large number of small diameters arranged in a straight line to face a chromatography support, etc., and the other end of the optical fiber arranged in a circumferential manner. are lined up to face a light-shielding disc having a through hole,
By rotating the light-shielding disk with a motor, the through holes 11iI are sequentially aligned with the other end of the optical fiber, and a light source section or a light-receiving section is installed on the opposite side of the light-shielding disk from the optical fiber. This makes it possible to irradiate or receive light from a minute portion on a chromatography support or the like through the through hole and the optical fiber, and to sequentially move the position of the minute portion. The present invention provides a scanning device for a densitometer in which the configuration of the scanning device is simplified,
Moreover, it becomes possible to reduce the time required for scanning compared to the conventional method.

[Brief explanation of the drawing]

FIG. 1 is an explanatory view of one embodiment of the device of the present invention, FIG. 2 is a diagram showing a scanning pattern in the device shown in FIG. 1, and FIG. FIG. 4 is a perspective view showing an example of the structure of the light source section, FIG. 5 is a diagram showing the main parts of another embodiment of the device of the present invention, and FIG. 6 is one end of the optical fiber. (1) - Scanning device of densitometer, (2) - 0.1 - Optical fiber, (2a) - (13a) - One side end of optical fiber, ( 2b) to (13b)...Other end of optical fiber, 0→...shading disk, 00...through hole, (1(9
...Pulse motor, (P)...4B chromatography plate, Vuno (G)...Minute part. 1. ・1 Agent Patent Attorney Nogawa Nogawa] Figure 3 Figure 4 Figure 5 Figure 6 3

Claims (1)

[Claims] 1. One end of an optical fiber having a large number of minute diameters is arranged in a straight line to face a chromatographic support, etc., and the other end is arranged in a circumferential manner and has a through hole. The light shielding disc is placed opposite to the light shielding disc, and the light shielding disc is rotated by a motor so that the through holes correspond to the respective other end portions of the optical fibers in sequence, and a light source section is provided on the opposite side of the optical fiber with respect to the light shielding disc. Alternatively, a light-receiving section is provided, which makes it possible to irradiate light to a minute portion on a chromatography support or the like through the through hole and optical fiber, or to receive light from a minute portion, and to sequentially move the position of the minute portion. A densitometer scanning device characterized by: 2. The device flc O 8 according to item 1, in which the chromatographic support and the like are mounted on a moving stage that is movable in a direction perpendicular to the direction in which one end of the optical fibers are lined up; 4. The apparatus according to claim 1, wherein the chromatographic support is a thin chromatographic plate; 4. The apparatus according to claim 1, wherein the chromatographic support is a paper chromatographic filter.
5. The apparatus according to claim 1, wherein the chromatographic support is agar for agar electrophoresis.